(Circular) (Six-base) MAPSORT of: pFN21AE2062.seq Check: 6057 from: 1 to: 7168 pFN21AE2062 7168 bp With 182 enzymes: SgfI * November 16, 2010 14:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4978 278 Size: 53 83 186 936 3442 2468 Fragments arranged by size: 3442 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2343 4041 4511 1937 Size: 406 1698 470 4594 Fragments arranged by size: 4594 1698 470 406 Acc65I G'GTAC_C Cuts at: 4489 4489 Size: 7168 AclI AA'CG_TT Cuts at: 1563 4250 5295 5668 1563 Size: 2687 1045 373 3063 Fragments arranged by size: 3063 2687 1045 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2062 3214 4172 4362 5231 6615 6883 1632 Size: 430 1152 958 190 869 1384 268 1917 Fragments arranged by size: 1917 1384 1152 958 869 430 268 190 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6971 AflIII A'CryG_T Cuts at: 1203 2730 6067 7044 1203 Size: 1527 3337 977 1327 Fragments arranged by size: 3337 1527 1327 977 AgeI A'CCGG_T Cuts at: 5999 5999 Size: 7168 AhdI GACnn_n'nnGTC Cuts at: 3814 5897 3814 Size: 2083 5085 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7168 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7168 AlwNI CAG_nnn'CTG Cuts at: 3092 4911 6483 3092 Size: 1819 1572 3777 Fragments arranged by size: 3777 1819 1572 ApaI G_GGCC'C Cuts at: 2247 2913 2247 Size: 666 6502 ApaLI G'TGCA_C Cuts at: 2234 3248 5225 6381 2234 Size: 1014 1977 1156 3021 Fragments arranged by size: 3021 1977 1156 1014 ApoI r'AATT_y Cuts at: 1493 4281 4477 4703 4855 6964 1493 Size: 2788 196 226 152 2109 1697 Fragments arranged by size: 2788 2109 1697 226 196 152 AseI AT'TA_AT Cuts at: 160 1032 5722 160 Size: 872 4690 1606 Fragments arranged by size: 4690 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7168 AvaI C'yCGr_G Cuts at: 1870 1943 3749 4493 1870 Size: 73 1806 744 4545 Fragments arranged by size: 4545 1806 744 73 BamHI G'GATC_C Cuts at: 4498 4865 4498 Size: 367 6801 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2291 4489 5944 618 Size: 325 217 627 504 2198 1455 1842 Fragments arranged by size: 2198 1842 1455 627 504 325 217 BanII G_rGCy'C Cuts at: 1391 2247 2913 3739 4109 4487 1391 Size: 856 666 826 370 378 4072 Fragments arranged by size: 4072 856 826 666 378 370 BbeI G_GCGC'C Cuts at: 2295 2295 Size: 7168 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3151 4275 928 Size: 971 1252 1124 3821 Fragments arranged by size: 3821 1252 1124 971 BbvCI CC'TCA_GC Cuts at: 4319 4319 Size: 7168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2591 2625 5359 5393 1903 Size: 34 654 34 2734 34 3678 Fragments arranged by size: 3678 2734 654 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2086 3278 4449 5060 6270 2086 Size: 1192 1171 611 1210 2984 Fragments arranged by size: 2984 1210 1192 1171 611 BclI T'GATC_A Cuts at: 1548 2211 1548 Size: 663 6505 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2767 2917 5779 Size: 107 122 71 631 605 1095 150 2862 Cuts at: 5779 136 Size: 1525 Fragments arranged by size: 2862 1525 1095 631 605 150 122 107 71 BglII A'GATC_T Cuts at: 4089 7163 4089 Size: 3074 4094 BlpI GC'TnA_GC Cuts at: 4585 4585 Size: 7168 Bme1580I G_kGCm'C Cuts at: 1790 2238 2247 2913 3252 4236 5229 6385 1790 Size: 448 9 666 339 984 993 1156 2573 Fragments arranged by size: 2573 1156 993 984 666 448 339 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3461 4899 5857 Size: 416 53 83 399 24 2044 1438 958 Cuts at: 5857 442 Size: 1753 Fragments arranged by size: 2044 1753 1438 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7168 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3325 3721 3746 3325 Size: 396 25 6747 Fragments arranged by size: 6747 396 25 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2665 3152 4002 4386 5828 1239 Size: 1426 487 850 384 1442 2579 Fragments arranged by size: 2579 1442 1426 850 487 384 Bpu10I CC'TnA_GC Cuts at: 4319 4319 Size: 7168 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2915 2924 5293 6158 6456 2915 Size: 9 2369 865 298 3627 Fragments arranged by size: 3627 2369 865 298 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3397 4237 5831 882 Size: 617 1898 840 1594 2219 Fragments arranged by size: 2219 1898 1594 840 617 BsaAI yAC'GTr Cuts at: 493 1204 2155 2733 2884 3027 4959 493 Size: 711 951 578 151 143 1932 2702 Fragments arranged by size: 2702 1932 951 711 578 151 143 BsaBI GATnn'nnATC Cuts at: 7162 7162 Size: 7168 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2292 4975 5357 5992 Size: 53 83 186 936 759 2683 382 635 Cuts at: 5992 275 Size: 1451 Fragments arranged by size: 2683 1451 936 759 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2393 5601 5999 6273 6420 6831 2393 Size: 3208 398 274 147 411 2730 Fragments arranged by size: 3208 2730 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2808 2838 1178 Size: 30 165 30 1405 30 5508 Fragments arranged by size: 5508 1405 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2832 3301 3803 1186 Size: 1646 469 502 4551 Fragments arranged by size: 4551 1646 502 469 BseYI C'CCAG_C Cuts at: 2013 2542 2856 3014 6371 6867 2013 Size: 529 314 158 3357 496 2314 Fragments arranged by size: 3357 2314 529 496 314 158 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3649 4171 1163 Size: 573 1913 522 4160 Fragments arranged by size: 4160 1913 573 522 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2759 4618 5379 6407 1540 Size: 275 186 758 1859 761 1028 2301 Fragments arranged by size: 2301 1859 1028 761 758 275 186 BsiHKAI G_wGCw'C Cuts at: 2238 2464 3252 5229 5314 6385 2238 Size: 226 788 1977 85 1071 3021 Fragments arranged by size: 3021 1977 1071 788 226 85 BsmI GAATG_Cn' Cuts at: 4679 4772 4679 Size: 93 7075 BsmBI CGTCTCn'nnnn_ Cuts at: 2814 2814 Size: 7168 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2238 2247 2464 2913 3252 3739 4109 Size: 399 448 9 217 449 339 487 370 Cuts at: 4109 4236 4487 5229 5314 6385 1391 Size: 127 251 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 449 448 399 370 339 251 217 127 85 9 BspEI T'CCGG_A Cuts at: 2393 6831 2393 Size: 4438 2730 BspHI T'CATG_A Cuts at: 5057 6787 5057 Size: 1730 5438 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4523 844 Size: 3679 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5055 1394 Size: 429 3232 3507 Fragments arranged by size: 3507 3232 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5663 5837 65 Size: 1183 4415 174 1396 Fragments arranged by size: 4415 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2766 2791 2948 3241 5812 5999 1700 Size: 110 956 25 157 293 2571 187 2869 Fragments arranged by size: 2869 2571 956 293 187 157 110 25 BsrGI T'GTAC_A Cuts at: 96 4200 96 Size: 4104 3064 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7168 BssSI C'ACGA_G Cuts at: 5228 6240 5228 Size: 1012 6156 BstAPI GCAn_nnn'nTGC Cuts at: 794 4231 794 Size: 3437 3731 BstBI TT'CG_AA Cuts at: 5972 5972 Size: 7168 BstEII G'GTnAC_C Cuts at: 1184 2041 3538 1184 Size: 857 1497 4814 Fragments arranged by size: 4814 1497 857 BstXI CCAn_nnnn'nTGG Cuts at: 3844 4008 3844 Size: 164 7004 BstYI r'GATC_y Cuts at: 1972 4089 4498 4865 5250 5267 6719 6805 6817 Size: 2117 409 367 385 17 1452 86 12 Cuts at: 6817 7163 1972 Size: 346 1977 Fragments arranged by size: 2117 1977 1452 409 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4042 4042 Size: 7168 Bsu36I CC'TnA_GG Cuts at: 3786 3786 Size: 7168 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 2961 3543 4128 513 Size: 552 939 42 915 582 585 3553 Fragments arranged by size: 3553 939 915 585 582 552 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2594 487 Size: 683 56 1368 5061 Fragments arranged by size: 5061 1368 683 56 BtsI GCAGTG_nn' Cuts at: 3510 4236 4462 4684 5478 5498 3510 Size: 726 226 222 794 20 5180 Fragments arranged by size: 5180 794 726 226 222 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7168 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7133 DraI TTT'AAA Cuts at: 4472 4472 Size: 7168 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6175 809 Size: 507 4859 1802 Fragments arranged by size: 4859 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2517 3546 3834 Size: 54 1425 50 275 18 687 1029 288 Cuts at: 3834 4615 5504 7133 8 Size: 781 889 1629 43 Fragments arranged by size: 1629 1425 1029 889 781 687 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4615 1537 Size: 275 2803 4090 Fragments arranged by size: 4090 2803 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 3091 3364 5098 1353 Size: 753 985 273 1734 3423 Fragments arranged by size: 3423 1734 985 753 273 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3512 5755 6129 6275 387 Size: 1064 138 1923 2243 374 146 1280 Fragments arranged by size: 2243 1923 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2665 3152 3214 4002 4172 4362 Size: 393 430 603 487 62 788 170 190 Cuts at: 4362 4386 5231 5828 6615 6883 1239 Size: 24 845 597 787 268 1524 Fragments arranged by size: 1524 845 788 787 603 597 487 430 393 268 190 170 62 24 EcoO109I rG'GnC_Cy Cuts at: 2243 3415 2243 Size: 1172 5996 EcoRI G'AATT_C Cuts at: 4281 4477 4855 4281 Size: 196 378 6594 Fragments arranged by size: 6594 378 196 EcoRV GAT'ATC Cuts at: 3054 3955 3054 Size: 901 6267 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3244 4386 3244 Size: 1142 6026 FspI TGC'GCA Cuts at: 5674 5674 Size: 7168 HaeII r_GCGC'y Cuts at: 1678 2295 3092 6315 1678 Size: 617 797 3223 2531 Fragments arranged by size: 3223 2531 797 617 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2372 2404 3584 3616 3800 Size: 32 135 32 800 32 1180 32 184 Cuts at: 3800 3832 4049 4081 5809 5841 5883 5915 1373 Size: 32 217 32 1728 32 42 32 2626 Fragments arranged by size: 2626 1728 1180 800 217 184 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2956 3075 4512 4758 669 Size: 988 281 1018 119 1437 246 3079 Fragments arranged by size: 3079 1437 1018 988 281 246 119 HindIII A'AGCT_T Cuts at: 748 748 Size: 7168 HpaI GTT'AAC Cuts at: 2956 4758 2956 Size: 1802 5366 KasI G'GCGC_C Cuts at: 2291 2291 Size: 7168 KpnI G_GTAC'C Cuts at: 4493 4493 Size: 7168 MfeI C'AATT_G Cuts at: 4767 4767 Size: 7168 MluI A'CGCG_T Cuts at: 7044 7044 Size: 7168 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2198 2794 3348 6282 6466 1305 Size: 893 596 554 2934 184 2007 Fragments arranged by size: 2934 2007 893 596 554 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3836 10 Size: 54 1425 343 2004 3342 Fragments arranged by size: 3342 2004 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2742 3377 4053 4208 5126 5485 5644 Size: 648 1576 635 676 155 918 359 159 Cuts at: 5644 518 Size: 2042 Fragments arranged by size: 2042 1576 918 676 648 635 359 159 155 MspA1I CmG'CkG Cuts at: 1606 2013 3334 3832 4584 5261 6409 6654 1606 Size: 407 1321 498 752 677 1148 245 2120 Fragments arranged by size: 2120 1321 1148 752 677 498 407 245 NaeI GCC'GGC Cuts at: 2793 3243 2793 Size: 450 6718 NarI GG'CG_CC Cuts at: 2292 2292 Size: 7168 NcoI C'CATG_G Cuts at: 513 1065 2004 2046 513 Size: 552 939 42 5635 Fragments arranged by size: 5635 939 552 42 NdeI CA'TA_TG Cuts at: 387 387 Size: 7168 NgoMIV G'CCGG_C Cuts at: 2791 3241 2791 Size: 450 6718 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7168 NotI GC'GGCC_GC Cuts at: 4615 4615 Size: 7168 NspI r_CATG'y Cuts at: 1132 1229 4229 4526 6071 1132 Size: 97 3000 297 1545 2229 Fragments arranged by size: 3000 2229 1545 297 97 PasI CC'CwG_GG Cuts at: 1348 3463 4118 1348 Size: 2115 655 4398 Fragments arranged by size: 4398 2115 655 PciI A'CATG_T Cuts at: 6067 6067 Size: 7168 PflMI CCAn_nnn'nTGG Cuts at: 1108 2574 3092 1108 Size: 1466 518 5184 Fragments arranged by size: 5184 1466 518 PmeI GTTT'AAAC Cuts at: 4472 4472 Size: 7168 PmlI CAC'GTG Cuts at: 2884 2884 Size: 7168 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5237 5269 6750 6782 5237 Size: 32 1481 32 5623 Fragments arranged by size: 5623 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3415 3415 Size: 7168 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7168 PsiI TTA'TAA Cuts at: 4738 4738 Size: 7168 PspOMI G'GGCC_C Cuts at: 2243 2909 2243 Size: 666 6502 PstI C_TGCA'G Cuts at: 830 4520 830 Size: 3690 3478 PvuI CG_AT'CG Cuts at: 2001 2759 2001 Size: 758 6410 PvuII CAG'CTG Cuts at: 2013 3832 2013 Size: 1819 5349 SalI G'TCGA_C Cuts at: 1936 4510 1936 Size: 2574 4594 SapI GCTCTTCn'nnn_ Cuts at: 2106 2106 Size: 7168 SbfI CC_TGCA'GG Cuts at: 4520 4520 Size: 7168 ScaI AGT'ACT Cuts at: 2276 3761 3851 5416 7128 2276 Size: 1485 90 1565 1712 2316 Fragments arranged by size: 2316 1712 1565 1485 90 SfcI C'TryA_G Cuts at: 826 1045 2340 4516 5651 6332 6523 826 Size: 219 1295 2176 1135 681 191 1471 Fragments arranged by size: 2176 1471 1295 1135 681 219 191 SfoI GGC'GCC Cuts at: 2293 2293 Size: 7168 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7168 SgrAI Cr'CCGG_yG Cuts at: 1700 2948 1700 Size: 1248 5920 SmaI CCC'GGG Cuts at: 1872 3751 4495 1872 Size: 1879 744 4545 Fragments arranged by size: 4545 1879 744 SmlI C'TyrA_G Cuts at: 820 1017 1943 2930 2939 5272 6173 6435 820 Size: 197 926 987 9 2333 901 262 1553 Fragments arranged by size: 2333 1553 987 926 901 262 197 9 SnaBI TAC'GTA Cuts at: 493 4959 493 Size: 4466 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7168 SphI G_CATG'C Cuts at: 1132 4229 4526 1132 Size: 3097 297 3774 Fragments arranged by size: 3774 3097 297 SspI AAT'ATT Cuts at: 5 52 5092 5 Size: 47 5040 2081 Fragments arranged by size: 5040 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7168 StyI C'CwwG_G Cuts at: 513 1065 2004 2046 3528 4607 513 Size: 552 939 42 1482 1079 3074 Fragments arranged by size: 3074 1482 1079 939 552 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5195 5212 5365 5550 5995 325 Size: 1255 3615 17 153 185 445 1498 Fragments arranged by size: 3615 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2274 2324 3759 Size: 274 80 33 51 1443 297 50 1435 Cuts at: 3759 3849 4200 5414 7126 96 Size: 90 351 1214 1712 138 Fragments arranged by size: 1712 1443 1435 1214 351 297 274 138 90 80 51 50 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3011 3043 7128 7160 3011 Size: 32 4085 32 3019 Fragments arranged by size: 4085 3019 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7168 XbaI T'CTAG_A Cuts at: 4504 4504 Size: 7168 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2571 3331 3898 2571 Size: 760 567 5841 Fragments arranged by size: 5841 760 567 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7168 XmaI C'CCGG_G Cuts at: 1870 3749 4493 1870 Size: 1879 744 4545 Fragments arranged by size: 4545 1879 744 XmnI GAAnn'nnTTC Cuts at: 5297 5297 Size: 7168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4976 276 Size: 53 83 186 936 3442 2468 Fragments arranged by size: 3442 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI DraIII EcoICRI EcoNI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI