Restriction map for pFN21AE2062
 (Linear) (Six-base) MAPSORT of: KIEE2062.seq  Check: 738  from: 1  to: 2463



KIEE2062.orf

 With 182 enzymes: SgfI * 

                        July 8, 2010 13:19  ..

AccI GT'mk_AC

Cuts at:      0     338    2036    2463 
   Size:        338    1698     427

  Fragments arranged by size: 

                1698     427     338
 
AclI AA'CG_TT

Cuts at:      0    2245    2463 
   Size:       2245     218
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57    1209    2167    2357    2463 
   Size:         57    1152     958     190     106

  Fragments arranged by size: 

                1152     958     190     106      57
 
AflIII A'CryG_T

Cuts at:      0     725    2463 
   Size:        725    1738
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1809    2463 
   Size:       1809     654
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1087    2463 
   Size:       1087    1376
 
ApaI G_GGCC'C

Cuts at:      0     242     908    2463 
   Size:        242     666    1555

  Fragments arranged by size: 

                1555     666     242
 
ApaLI G'TGCA_C

Cuts at:      0     229    1243    2463 
   Size:        229    1014    1220

  Fragments arranged by size: 

                1220    1014     229
 
ApoI r'AATT_y

Cuts at:      0    2276    2463 
   Size:       2276     187
 
AvaI C'yCGr_G

Cuts at:      0    1744    2463 
   Size:       1744     719
 
BanI G'GyrC_C

Cuts at:      0     286    2463 
   Size:        286    2177
 
BanII G_rGCy'C

Cuts at:      0     242     908    1734    2104    2463 
   Size:        242     666     826     370     359

  Fragments arranged by size: 

                 826     666     370     359     242
 
BbeI G_GCGC'C

Cuts at:      0     290    2463 
   Size:        290    2173
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1146    2270    2463 
   Size:       1146    1124     193

  Fragments arranged by size: 

                1146    1124     193
 
BbvCI CC'TCA_GC

Cuts at:      0    2314    2463 
   Size:       2314     149
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     586     620    2463 
   Size:        586      34    1843

  Fragments arranged by size: 

                1843     586      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      81    1273    2444    2463 
   Size:         81    1192    1171      19

  Fragments arranged by size: 

                1192    1171      81      19
 
BclI T'GATC_A

Cuts at:      0     206    2463 
   Size:        206    2257
 
BglI GCCn_nnn'nGGC

Cuts at:      0     762     912    2463 
   Size:        762     150    1551

  Fragments arranged by size: 

                1551     762     150
 
BglII A'GATC_T

Cuts at:      0    2084    2463 
   Size:       2084     379
 
Bme1580I G_kGCm'C

Cuts at:      0     233     242     908    1247    2231    2463 
   Size:        233       9     666     339     984     232

  Fragments arranged by size: 

                 984     666     339     233     232       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1456    2463 
   Size:       1456    1007
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1320    1716    1741    2463 
   Size:       1320     396      25     722

  Fragments arranged by size: 

                1320     722     396      25
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     660    1147    1997    2381    2463 
   Size:        660     487     850     384      82

  Fragments arranged by size: 

                 850     660     487     384      82
 
Bpu10I CC'TnA_GC

Cuts at:      0    2314    2463 
   Size:       2314     149
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     910     919    2463 
   Size:        910       9    1544

  Fragments arranged by size: 

                1544     910       9
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1392    2232    2463 
   Size:       1392     840     231

  Fragments arranged by size: 

                1392     840     231
 
BsaAI yAC'GTr

Cuts at:      0     150     728     879    1022    2463 
   Size:        150     578     151     143    1441

  Fragments arranged by size: 

                1441     578     151     150     143
 
BsaHI Gr'CG_yC

Cuts at:      0     287    2463 
   Size:        287    2176
 
BsaWI w'CCGG_w

Cuts at:      0     388    2463 
   Size:        388    2075
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     803     833    2463 
   Size:        803      30    1630

  Fragments arranged by size: 

                1630     803      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     827    1296    1798    2463 
   Size:        827     469     502     665

  Fragments arranged by size: 

                 827     665     502     469
 
BseYI C'CCAG_C

Cuts at:      0       8     537     851    1009    2463 
   Size:          8     529     314     158    1454

  Fragments arranged by size: 

                1454     529     314     158       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1644    2166    2463 
   Size:       1644     522     297

  Fragments arranged by size: 

                1644     522     297
 
BsiEI CG_ry'CG

Cuts at:      0     754    2463 
   Size:        754    1709
 
BsiHKAI G_wGCw'C

Cuts at:      0     233     459    1247    2463 
   Size:        233     226     788    1216

  Fragments arranged by size: 

                1216     788     233     226
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     809    2463 
   Size:        809    1654
 
Bsp1286I G_dGCh'C

Cuts at:      0     233     242     459     908    1247    1734    2104    2231 
   Size:        233       9     217     449     339     487     370     127

Cuts at:   2231    2463 
   Size:        232

  Fragments arranged by size: 

                 487     449     370     339     233     232     217     127
                   9
 
BspEI T'CCGG_A

Cuts at:      0     388    2463 
   Size:        388    2075
 
BsrFI r'CCGG_y

Cuts at:      0     761     786     943    1236    2463 
   Size:        761      25     157     293    1227

  Fragments arranged by size: 

                1227     761     293     157      25
 
BsrGI T'GTAC_A

Cuts at:      0    2195    2463 
   Size:       2195     268
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2226    2463 
   Size:       2226     237
 
BstEII G'GTnAC_C

Cuts at:      0      36    1533    2463 
   Size:         36    1497     930

  Fragments arranged by size: 

                1497     930      36
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1839    2003    2463 
   Size:       1839     164     460

  Fragments arranged by size: 

                1839     460     164
 
BstYI r'GATC_y

Cuts at:      0    2084    2463 
   Size:       2084     379
 
BstZ17I GTA'TAC

Cuts at:      0    2037    2463 
   Size:       2037     426
 
Bsu36I CC'TnA_GG

Cuts at:      0    1781    2463 
   Size:       1781     682
 
BtgI C'CryG_G

Cuts at:      0      41     956    1538    2123    2463 
   Size:         41     915     582     585     340

  Fragments arranged by size: 

                 915     585     582     340      41
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     589    2463 
   Size:        589    1874
 
BtsI GCAGTG_nn'

Cuts at:      0    1505    2231    2457    2463 
   Size:       1505     726     226       6

  Fragments arranged by size: 

                1505     726     226       6
 
EaeI y'GGCC_r

Cuts at:      0     512    1541    1829    2463 
   Size:        512    1029     288     634

  Fragments arranged by size: 

                1029     634     512     288
 
EarI CTCTTCn'nnn_

Cuts at:      0     101    1086    1359    2463 
   Size:        101     985     273    1104

  Fragments arranged by size: 

                1104     985     273     101
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1507    2463 
   Size:       1507     956
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     660    1147    1209    1997    2167    2357    2381 
   Size:         57     603     487      62     788     170     190      24

Cuts at:   2381    2463 
   Size:         82

  Fragments arranged by size: 

                 788     603     487     190     170      82      62      57
                  24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     238    1410    2463 
   Size:        238    1172    1053

  Fragments arranged by size: 

                1172    1053     238
 
EcoRI G'AATT_C

Cuts at:      0    2276    2463 
   Size:       2276     187
 
EcoRV GAT'ATC

Cuts at:      0    1049    1950    2463 
   Size:       1049     901     513

  Fragments arranged by size: 

                1049     901     513
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1239    2381    2463 
   Size:       1239    1142      82

  Fragments arranged by size: 

                1239    1142      82
 
HaeII r_GCGC'y

Cuts at:      0     290    1087    2463 
   Size:        290     797    1376

  Fragments arranged by size: 

                1376     797     290
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     367     399    1579    1611    1795    1827    2044    2076 
   Size:        367      32    1180      32     184      32     217      32

Cuts at:   2076    2463 
   Size:        387

  Fragments arranged by size: 

                1180     387     367     217     184      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     951    1070    2463 
   Size:        951     119    1393

  Fragments arranged by size: 

                1393     951     119
 
HpaI GTT'AAC

Cuts at:      0     951    2463 
   Size:        951    1512
 
KasI G'GCGC_C

Cuts at:      0     286    2463 
   Size:        286    2177
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     789    1343    2463 
   Size:        193     596     554    1120

  Fragments arranged by size: 

                1120     596     554     193
 
MscI TGG'CCA

Cuts at:      0    1831    2463 
   Size:       1831     632
 
MslI CAynn'nnrTG

Cuts at:      0     737    1372    2048    2203    2463 
   Size:        737     635     676     155     260

  Fragments arranged by size: 

                 737     676     635     260     155
 
MspA1I CmG'CkG

Cuts at:      0       8    1329    1827    2463 
   Size:          8    1321     498     636

  Fragments arranged by size: 

                1321     636     498       8
 
NaeI GCC'GGC

Cuts at:      0     788    1238    2463 
   Size:        788     450    1225

  Fragments arranged by size: 

                1225     788     450
 
NarI GG'CG_CC

Cuts at:      0     287    2463 
   Size:        287    2176
 
NcoI C'CATG_G

Cuts at:      0      41    2463 
   Size:         41    2422
 
NgoMIV G'CCGG_C

Cuts at:      0     786    1236    2463 
   Size:        786     450    1227

  Fragments arranged by size: 

                1227     786     450
 
NspI r_CATG'y

Cuts at:      0    2224    2463 
   Size:       2224     239
 
PasI CC'CwG_GG

Cuts at:      0    1458    2113    2463 
   Size:       1458     655     350

  Fragments arranged by size: 

                1458     655     350
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     569    1087    2463 
   Size:        569     518    1376

  Fragments arranged by size: 

                1376     569     518
 
PmlI CAC'GTG

Cuts at:      0     879    2463 
   Size:        879    1584
 
PpuMI rG'GwC_Cy

Cuts at:      0    1410    2463 
   Size:       1410    1053
 
PspOMI G'GGCC_C

Cuts at:      0     238     904    2463 
   Size:        238     666    1559

  Fragments arranged by size: 

                1559     666     238
 
PvuI CG_AT'CG

Cuts at:      0     754    2463 
   Size:        754    1709
 
PvuII CAG'CTG

Cuts at:      0       8    1827    2463 
   Size:          8    1819     636

  Fragments arranged by size: 

                1819     636       8
 
SapI GCTCTTCn'nnn_

Cuts at:      0     101    2463 
   Size:        101    2362
 
ScaI AGT'ACT

Cuts at:      0     271    1756    1846    2463 
   Size:        271    1485      90     617

  Fragments arranged by size: 

                1485     617     271      90
 
SfcI C'TryA_G

Cuts at:      0     335    2463 
   Size:        335    2128
 
SfoI GGC'GCC

Cuts at:      0     288    2463 
   Size:        288    2175
 
SgrAI Cr'CCGG_yG

Cuts at:      0     943    2463 
   Size:        943    1520
 
SmaI CCC'GGG

Cuts at:      0    1746    2463 
   Size:       1746     717
 
SmlI C'TyrA_G

Cuts at:      0     925     934    2463 
   Size:        925       9    1529

  Fragments arranged by size: 

                1529     925       9
 
SphI G_CATG'C

Cuts at:      0    2224    2463 
   Size:       2224     239
 
StyI C'CwwG_G

Cuts at:      0      41    1523    2463 
   Size:         41    1482     940

  Fragments arranged by size: 

                1482     940      41
 
TatI w'GTAC_w

Cuts at:      0     269     319    1754    1844    2195    2463 
   Size:        269      50    1435      90     351     268

  Fragments arranged by size: 

                1435     351     269     268      90      50
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1006    1038    2463 
   Size:       1006      32    1425

  Fragments arranged by size: 

                1425    1006      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     566    1326    1893    2463 
   Size:        566     760     567     570

  Fragments arranged by size: 

                 760     570     567     566
 
XmaI C'CCGG_G

Cuts at:      0    1744    2463 
   Size:       1744     719
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AhdI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BsrFI
    BsrGI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII     HpaI     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI    PflMI     PmlI    PpuMI   PspOMI     PvuI    PvuII     SapI
     ScaI     SfcI     SfoI    SgrAI     SmaI     SmlI     SphI     StyI
     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AleI     AlfI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BfrBI
     BlpI    BmgBI     BmtI    BsaBI    BsiWI     BsmI    BspHI    BspMI
    BsrBI    BsrDI   BssHII    BssSI    BstBI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoNI     FseI     FspI    FspAI
  HindIII     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PfoI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI     PstI    RsrII     SacI    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SgfI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmnI     ZraI
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