(Linear) (Six-base) MAPSORT of: KIEE1245.seq Check: 7594 from: 1 to: 1431 KIEE1245.orf With 182 enzymes: SgfI * March 22, 2012 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 750 1431 Size: 750 681 AleI CACnn'nnGTG Cuts at: 0 352 1431 Size: 352 1079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 202 1431 Size: 202 1229 AlwNI CAG_nnn'CTG Cuts at: 0 504 1167 1431 Size: 504 663 264 Fragments arranged by size: 663 504 264 ApaLI G'TGCA_C Cuts at: 0 537 1431 Size: 537 894 AscI GG'CGCG_CC Cuts at: 0 616 1431 Size: 616 815 AvaI C'yCGr_G Cuts at: 0 148 1233 1270 1380 1431 Size: 148 1085 37 110 51 Fragments arranged by size: 1085 148 110 51 37 BanI G'GyrC_C Cuts at: 0 25 99 333 1059 1199 1431 Size: 25 74 234 726 140 232 Fragments arranged by size: 726 234 232 140 74 25 BanII G_rGCy'C Cuts at: 0 38 1340 1431 Size: 38 1302 91 Fragments arranged by size: 1302 91 38 BbeI G_GCGC'C Cuts at: 0 29 1203 1431 Size: 29 1174 228 Fragments arranged by size: 1174 228 29 BbsI GAAGACnn'nnnn_ Cuts at: 0 987 1071 1431 Size: 987 84 360 Fragments arranged by size: 987 360 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1154 1188 1431 Size: 1154 34 243 Fragments arranged by size: 1154 243 34 BglI GCCn_nnn'nGGC Cuts at: 0 85 556 901 1108 1406 1431 Size: 85 471 345 207 298 25 Fragments arranged by size: 471 345 298 207 85 25 Bme1580I G_kGCm'C Cuts at: 0 104 541 707 1064 1431 Size: 104 437 166 357 367 Fragments arranged by size: 437 367 357 166 104 BmgBI CAC'GTC Cuts at: 0 414 1431 Size: 414 1017 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 396 1431 Size: 396 1035 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1354 1431 Size: 1354 77 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 952 1431 Size: 952 479 BsaI GGTCTCn'nnnn_ Cuts at: 0 433 1211 1431 Size: 433 778 220 Fragments arranged by size: 778 433 220 BsaAI yAC'GTr Cuts at: 0 702 1431 Size: 702 729 BsaHI Gr'CG_yC Cuts at: 0 26 256 992 1200 1431 Size: 26 230 736 208 231 Fragments arranged by size: 736 231 230 208 26 BseYI C'CCAG_C Cuts at: 0 368 804 975 1092 1431 Size: 368 436 171 117 339 Fragments arranged by size: 436 368 339 171 117 BsiEI CG_ry'CG Cuts at: 0 56 814 1068 1225 1431 Size: 56 758 254 157 206 Fragments arranged by size: 758 254 206 157 56 BsiHKAI G_wGCw'C Cuts at: 0 17 541 1431 Size: 17 524 890 Fragments arranged by size: 890 524 17 Bsp1286I G_dGCh'C Cuts at: 0 17 38 104 541 707 1064 1340 1431 Size: 17 21 66 437 166 357 276 91 Fragments arranged by size: 437 357 276 166 91 66 21 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 740 750 1431 Size: 740 10 681 Fragments arranged by size: 740 681 10 BsrBI CCG'CTC Cuts at: 0 63 1431 Size: 63 1368 BsrDI GCAATG_nn' Cuts at: 0 231 452 1431 Size: 231 221 979 Fragments arranged by size: 979 231 221 BsrFI r'CCGG_y Cuts at: 0 1196 1220 1345 1431 Size: 1196 24 125 86 Fragments arranged by size: 1196 125 86 24 BssHII G'CGCG_C Cuts at: 0 513 515 616 1178 1180 1227 1324 1431 Size: 513 2 101 562 2 47 97 107 Fragments arranged by size: 562 513 107 101 97 47 2 2 BssSI C'ACGA_G Cuts at: 0 532 940 1431 Size: 532 408 491 Fragments arranged by size: 532 491 408 BstEII G'GTnAC_C Cuts at: 0 40 1431 Size: 40 1391 BstXI CCAn_nnnn'nTGG Cuts at: 0 354 1431 Size: 354 1077 BtgI C'CryG_G Cuts at: 0 83 259 1212 1431 Size: 83 176 953 219 Fragments arranged by size: 953 219 176 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 231 660 1250 1431 Size: 231 429 590 181 Fragments arranged by size: 590 429 231 181 BtsI GCAGTG_nn' Cuts at: 0 172 1431 Size: 172 1259 DraIII CAC_nnn'GTG Cuts at: 0 545 1431 Size: 545 886 DrdI GACnn_nn'nnGTC Cuts at: 0 1300 1303 1431 Size: 1300 3 128 Fragments arranged by size: 1300 128 3 EaeI y'GGCC_r Cuts at: 0 53 77 242 299 428 811 902 1025 Size: 53 24 165 57 129 383 91 123 Cuts at: 1025 1222 1431 Size: 197 209 Fragments arranged by size: 383 209 197 165 129 123 91 57 53 24 EagI C'GGCC_G Cuts at: 0 53 811 1222 1431 Size: 53 758 411 209 Fragments arranged by size: 758 411 209 53 EarI CTCTTCn'nnn_ Cuts at: 0 289 1078 1431 Size: 289 789 353 Fragments arranged by size: 789 353 289 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 616 1384 1431 Size: 616 768 47 Fragments arranged by size: 768 616 47 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1354 1431 Size: 1354 77 EcoO109I rG'GnC_Cy Cuts at: 0 528 1431 Size: 528 903 HaeII r_GCGC'y Cuts at: 0 29 199 308 789 967 1203 1431 Size: 29 170 109 481 178 236 228 Fragments arranged by size: 481 236 228 178 170 109 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 142 174 1363 1395 1431 Size: 142 32 1189 32 36 Fragments arranged by size: 1189 142 36 32 32 KasI G'GCGC_C Cuts at: 0 25 1199 1431 Size: 25 1174 232 Fragments arranged by size: 1174 232 25 MscI TGG'CCA Cuts at: 0 244 301 904 1027 1431 Size: 244 57 603 123 404 Fragments arranged by size: 603 404 244 123 57 MslI CAynn'nnrTG Cuts at: 0 352 355 583 985 1431 Size: 352 3 228 402 446 Fragments arranged by size: 446 402 352 228 3 MspA1I CmG'CkG Cuts at: 0 85 156 175 261 368 623 779 900 Size: 85 71 19 86 107 255 156 121 Cuts at: 900 1431 Size: 531 Fragments arranged by size: 531 255 156 121 107 86 85 71 19 NaeI GCC'GGC Cuts at: 0 1198 1347 1431 Size: 1198 149 84 Fragments arranged by size: 1198 149 84 NarI GG'CG_CC Cuts at: 0 26 1200 1431 Size: 26 1174 231 Fragments arranged by size: 1174 231 26 NgoMIV G'CCGG_C Cuts at: 0 1196 1345 1431 Size: 1196 149 86 Fragments arranged by size: 1196 149 86 NotI GC'GGCC_GC Cuts at: 0 811 1431 Size: 811 620 PasI CC'CwG_GG Cuts at: 0 1340 1431 Size: 1340 91 PspXI vC'TCGA_Gb Cuts at: 0 1380 1431 Size: 1380 51 PstI C_TGCA'G Cuts at: 0 269 1431 Size: 269 1162 PvuII CAG'CTG Cuts at: 0 779 1431 Size: 779 652 SacII CC_GC'GG Cuts at: 0 86 262 1431 Size: 86 176 1169 Fragments arranged by size: 1169 176 86 SexAI A'CCwGG_T Cuts at: 0 311 1431 Size: 311 1120 SfcI C'TryA_G Cuts at: 0 265 1431 Size: 265 1166 SfiI GGCCn_nnn'nGGCC Cuts at: 0 556 1431 Size: 556 875 SfoI GGC'GCC Cuts at: 0 27 1201 1431 Size: 27 1174 230 Fragments arranged by size: 1174 230 27 SgrAI Cr'CCGG_yG Cuts at: 0 1196 1431 Size: 1196 235 SmaI CCC'GGG Cuts at: 0 1272 1431 Size: 1272 159 SmlI C'TyrA_G Cuts at: 0 967 1380 1431 Size: 967 413 51 Fragments arranged by size: 967 413 51 StuI AGG'CCT Cuts at: 0 1042 1156 1431 Size: 1042 114 275 Fragments arranged by size: 1042 275 114 StyI C'CwwG_G Cuts at: 0 1424 1431 Size: 1424 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 403 435 1381 1413 1431 Size: 403 32 946 32 18 Fragments arranged by size: 946 403 32 32 18 Tth111I GACn'n_nGTC Cuts at: 0 313 652 1300 1431 Size: 313 339 648 131 Fragments arranged by size: 648 339 313 131 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 309 558 897 1431 Size: 309 249 339 534 Fragments arranged by size: 534 339 309 249 XhoI C'TCGA_G Cuts at: 0 1380 1431 Size: 1380 51 XmaI C'CCGG_G Cuts at: 0 1270 1431 Size: 1270 161 XmnI GAAnn'nnTTC Cuts at: 0 1042 1431 Size: 1042 389 Enzymes that do cut: AarI AleI AlfI AlwNI ApaLI AscI AvaI BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaAI BsaHI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstEII BstXI BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV NotI PasI PspXI PstI PvuII SacII SexAI SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AloI ApaI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmrI BmtI Bpu10I BsaBI BsaWI BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrGI BstAPI BstBI BstYI BstZ17I Bsu36I ClaI CspCI DraI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SgfI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI XbaI ZraI