Restriction map for pFN21AE5279
 (Circular) (Six-base) MAPSORT of: pFN21AE5279.seq  Check: 9012  from: 1  to: 5419



pFN21AE5279  5419 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:16  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3229     278 
   Size:         53      83     186     936    1693    2468

  Fragments arranged by size: 

                2468    1693     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2762    1937 
   Size:        825    4594
 
Acc65I G'GTAC_C

Cuts at:   2740    2740 
   Size:       5419
 
AclI AA'CG_TT

Cuts at:   1563    3546    3919    1563 
   Size:       1983     373    3063

  Fragments arranged by size: 

                3063    1983     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2367    3482    4866    5134    1632 
   Size:        735    1115    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1115     735     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5222
 
AflIII A'CryG_T

Cuts at:   1203    4318    5295    1203 
   Size:       3115     977    1327

  Fragments arranged by size: 

                3115    1327     977
 
AgeI A'CCGG_T

Cuts at:   4250    4250 
   Size:       5419
 
AhdI GACnn_n'nnGTC

Cuts at:   2152    4148    2152 
   Size:       1996    3423
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5419
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5419
 
AlwNI CAG_nnn'CTG

Cuts at:   3162    4734    3162 
   Size:       1572    3847
 
ApaLI G'TGCA_C

Cuts at:   3476    4632    3476 
   Size:       1156    4263
 
ApoI r'AATT_y

Cuts at:   1493    2728    2954    3106    5215    1493 
   Size:       1235     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1235     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3973     160 
   Size:        872    2941    1606

  Fragments arranged by size: 

                2941    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5419
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2744    1870 
   Size:         73     801    4545

  Fragments arranged by size: 

                4545     801      73
 
BamHI G'GATC_C

Cuts at:   2749    3116    2749 
   Size:        367    5052
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2309    2740    4195     618 
   Size:        325     217     627     522     431    1455    1842

  Fragments arranged by size: 

                1842    1455     627     522     431     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2738    1391 
   Size:       1347    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4448
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3610    3644    1903 
   Size:         34    1673      34    3678

  Fragments arranged by size: 

                3678    1673      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3311    4521    3311 
   Size:       1210    4209
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5419
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4030     136 
   Size:        107     122      71     631     605    2358    1525

  Fragments arranged by size: 

                2358    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5414    5414 
   Size:       5419
 
BlpI GC'TnA_GC

Cuts at:   2046    2836    2046 
   Size:        790    4629
 
Bme1580I G_kGCm'C

Cuts at:   1790    3480    4636    1790 
   Size:       1690    1156    2573

  Fragments arranged by size: 

                2573    1690    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5419
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3150    4108     442 
   Size:        416      53      83     399      24    1733     958    1753

  Fragments arranged by size: 

                1753    1733     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5419
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2036    2272    4079    1239 
   Size:        797     236    1807    2579

  Fragments arranged by size: 

                2579    1807     797     236
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2354    2525    2700    3544    4409    4707    2354 
   Size:        171     175     844     865     298    3066

  Fragments arranged by size: 

                3066     865     844     298     175     171
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4082     882 
   Size:        617    2583    2219

  Fragments arranged by size: 

                2583    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3210     493 
   Size:        711    2006    2702

  Fragments arranged by size: 

                2702    2006     711
 
BsaBI GATnn'nnATC

Cuts at:   5413    5413 
   Size:       5419
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3226    3608    4243     275 
   Size:         53      83     186     936    1693     382     635    1451

  Fragments arranged by size: 

                1693    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3852    4250    4524    4671    5082    3852 
   Size:        398     274     147     411    4189

  Fragments arranged by size: 

                4189     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5194

  Fragments arranged by size: 

                5194     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2303    2605    1186 
   Size:       1117     302    4000

  Fragments arranged by size: 

                4000    1117     302
 
BseYI C'CCAG_C

Cuts at:   2445    4622    5118    2445 
   Size:       2177     496    2746

  Fragments arranged by size: 

                2746    2177     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4846
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2869    3630    4658    1540 
   Size:        275     186     868     761    1028    2301

  Fragments arranged by size: 

                2301    1028     868     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3480    3565    4636    3480 
   Size:         85    1071    4263

  Fragments arranged by size: 

                4263    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2930    3023    2930 
   Size:         93    5326
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2165    2165 
   Size:       5419
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2738    3480    3565    4636    1391 
   Size:        399     948     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     948     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5082    5082 
   Size:       5419
 
BspHI T'CATG_A

Cuts at:   3308    5038    3308 
   Size:       1730    3689
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2774     844 
   Size:       1930    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3306    1394 
   Size:        429    1483    3507

  Fragments arranged by size: 

                3507    1483     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2347    3914    4088      65 
   Size:       1183    1099    1567     174    1396

  Fragments arranged by size: 

                1567    1396    1183    1099     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4063    4250    1700 
   Size:        110    2253     187    2869

  Fragments arranged by size: 

                2869    2253     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5419
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5419
 
BssSI C'ACGA_G

Cuts at:   3479    4491    3479 
   Size:       1012    4407
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2710     794 
   Size:       1916    3503
 
BstBI TT'CG_AA

Cuts at:   4223    4223 
   Size:       5419
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5419
 
BstYI r'GATC_y

Cuts at:   1972    2749    3116    3501    3518    4970    5056    5068    5414 
   Size:        777     367     385      17    1452      86      12     346

Cuts at:   5414    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     777     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4867
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4680

  Fragments arranged by size: 

                4680     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2935    3729    3749    2935 
   Size:        794      20    4605

  Fragments arranged by size: 

                4605     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5419
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5384
 
DraI TTT'AAA

Cuts at:   2723    2723 
   Size:       5419
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4426     809 
   Size:        507    3110    1802

  Fragments arranged by size: 

                3110    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2866    3755    5384 
   Size:         54    1425      50     275      18    1036     889    1629

Cuts at:   5384       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1036     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2866    1537 
   Size:        275    1054    4090

  Fragments arranged by size: 

                4090    1054     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3349    1353 
   Size:       1996    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4006    4380    4526     387 
   Size:       1064     138    2417     374     146    1280

  Fragments arranged by size: 

                2417    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2036    2272    2367    3482    4079    4866    5134 
   Size:        393     404     236      95    1115     597     787     268

Cuts at:   5134    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1115     787     597     404     393     268     236
                  95
 
EcoRI G'AATT_C

Cuts at:   2728    3106    2728 
   Size:        378    5041
 
FspI TGC'GCA

Cuts at:   3925    3925 
   Size:       5419
 
HaeII r_GCGC'y

Cuts at:   1678    4566    1678 
   Size:       2888    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2138    2170    2659    2691    4060 
   Size:         32     135      32     566      32     489      32    1369

Cuts at:   4060    4092    4134    4166    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1369     566     489     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2157    2763    3009     669 
   Size:        988     281     219     606     246    3079

  Fragments arranged by size: 

                3079     988     606     281     246     219
 
HindIII A'AGCT_T

Cuts at:    748    2472     748 
   Size:       1724    3695
 
HpaI GTT'AAC

Cuts at:   3009    3009 
   Size:       5419
 
KpnI G_GTAC'C

Cuts at:   2744    2744 
   Size:       5419
 
MfeI C'AATT_G

Cuts at:   3018    3018 
   Size:       5419
 
MluI A'CGCG_T

Cuts at:   5295    5295 
   Size:       5419
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2611    4533    4717    1305 
   Size:       1306    1922     184    2007

  Fragments arranged by size: 

                2007    1922    1306     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3597

  Fragments arranged by size: 

                3597    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3377    3736    3895     518 
   Size:        648    2211     359     159    2042

  Fragments arranged by size: 

                2211    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2029    2315    2835    3512    4660    4905    1606 
   Size:        423     286     520     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     520     423     286     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4867
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5419
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5419
 
NotI GC'GGCC_GC

Cuts at:   2866    2866 
   Size:       5419
 
NspI r_CATG'y

Cuts at:   1132    1229    2777    4322    1132 
   Size:         97    1548    1545    2229

  Fragments arranged by size: 

                2229    1548    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5419
 
PciI A'CATG_T

Cuts at:   4318    4318 
   Size:       5419
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5419
 
PmeI GTTT'AAAC

Cuts at:   2723    2723 
   Size:       5419
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3488    3520    5001    5033    3488 
   Size:         32    1481      32    3874

  Fragments arranged by size: 

                3874    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5419
 
PsiI TTA'TAA

Cuts at:   2989    2989 
   Size:       5419
 
PstI C_TGCA'G

Cuts at:    830    2771     830 
   Size:       1941    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5419
 
PvuII CAG'CTG

Cuts at:   2029    2029 
   Size:       5419
 
SalI G'TCGA_C

Cuts at:   1936    2761    1936 
   Size:        825    4594
 
SbfI CC_TGCA'GG

Cuts at:   2771    2771 
   Size:       5419
 
ScaI AGT'ACT

Cuts at:   3667    5379    3667 
   Size:       1712    3707
 
SfcI C'TryA_G

Cuts at:    826    1045    2376    2767    3902    4583    4774     826 
   Size:        219    1331     391    1135     681     191    1471

  Fragments arranged by size: 

                1471    1331    1135     681     391     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5419
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5419
 
SmaI CCC'GGG

Cuts at:   1872    2746    1872 
   Size:        874    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2369    2504    2715    3523    4424    4686 
   Size:        197     926     426     135     211     808     901     262

Cuts at:   4686     820 
   Size:       1553

  Fragments arranged by size: 

                1553     926     901     808     426     262     211     197
                 135
 
SnaBI TAC'GTA

Cuts at:    493    3210     493 
   Size:       2717    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5419
 
SphI G_CATG'C

Cuts at:   1132    2777    1132 
   Size:       1645    3774
 
SspI AAT'ATT

Cuts at:      5      52    3343       5 
   Size:         47    3291    2081

  Fragments arranged by size: 

                3291    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5419
 
StyI C'CwwG_G

Cuts at:    513    1065    2858     513 
   Size:        552    1793    3074

  Fragments arranged by size: 

                3074    1793     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3446    3463    3616    3801    4246     325 
   Size:       1255    1866      17     153     185     445    1498

  Fragments arranged by size: 

                1866    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3665    5377      96 
   Size:        274      80      33      51    1443    1688    1712     138

  Fragments arranged by size: 

                1712    1688    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5379    5411    5379 
   Size:         32    5387
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5419
 
XbaI T'CTAG_A

Cuts at:   2755    2755 
   Size:       5419
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5419
 
XmaI C'CCGG_G

Cuts at:   1870    2744    1870 
   Size:        874    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3548    3548 
   Size:       5419
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3227     276 
   Size:         53      83     186     936    1693    2468

  Fragments arranged by size: 

                2468    1693     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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