################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AclI AflII AgeI Aor51HI AscI AseI AsnI Asp718I BclI BfrI BlpI Bpu1102I BsaBI BsaI BsiEI BsiWI BsmBI BspLU11I BssHII Bst1107I BstBI BstZ17I CelII CpoI DraI EagI EclXI Eco47III Eco52I EcoRV FbaI FseI HgaI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SfiI SfuI SgrAI SmlI SnaBI SpeI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AccI 451 GTCTAC GT^[AC][GT]AC AccIII 2909 TCCGGA T^CCGGA AcyI 30 GGCGCC G[GA]^CG[CT]C AflIII 2063 ACGTGT A^C[GA][CT]GT AhdI 443 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 58 GTGCAC G^TGCAC AocI 2123 CCTCAGG CC^T[ACGT]AGG ApaLI 58 GTGCAC G^TGCAC AspEI 443 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3032 TGCGCA TGC^GCA AvrII 504 CCTAGG C^CTAGG BalI 2565 TGGCCA TGG^CCA BamHI 555 GGATCC G^GATCC BbeI 30 GGCGCC GGCGC^C BbrPI 2851 CACGTG CAC^GTG BcgI 1565 GCAGCCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 704 GTATCC GTATCC(6/5) BglI 366 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1422 AGATCT A^GATCT BlnI 504 CCTAGG C^CTAGG BsaAI 2851 CACGTG [CT]AC^GT[GA] BsaHI 30 GGCGCC G[GA]^CG[CT]C BseAI 2909 TCCGGA T^CCGGA BsmI 846 GAATGC GAATGC(1/-1) BspDI 670 ATCGAT AT^CGAT BspEI 2909 TCCGGA T^CCGGA BspHI 2840 TCATGA T^CATGA BspMI 1135 GCAGGT ACCTGC(4/8) BsrBI 943 GAGCGG CCGCTC(-3/-3) BsrDI 1172 GCAATG GCAATG(2/0) BsrFI 290 ACCGGC [GA]^CCGG[CT] BstAPI 2925 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1459 GGTCACC G^GT[ACGT]ACC BstPI 1459 GGTCACC G^GT[ACGT]ACC BstXI 3002 CCAAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2123 CCTCAGG CC^T[ACGT]AGG Cfr10I 290 ACCGGC [GA]^CCGG[CT] ClaI 670 ATCGAT AT^CGAT DraIII 2035 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2857 GACACCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 443 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2123 CCTCAGG CC^T[ACGT]AGG EcoO65I 1459 GGTCACC G^GT[ACGT]ACC EcoRI 2326 GAATTC G^AATTC EcoT22I 2932 ATGCAT ATGCA^T FspI 3032 TGCGCA TGC^GCA HaeII 30 GGCGCC [GA]GCGC^[CT] Hin1I 30 GGCGCC G[GA]^CG[CT]C KasI 30 GGCGCC G^GCGCC KspI 11 CCGCGG CCGC^GG MluNI 2565 TGGCCA TGG^CCA MroI 2909 TCCGGA T^CCGGA MscI 2565 TGGCCA TGG^CCA NarI 30 GGCGCC GG^CGCC NheI 2337 GCTAGC G^CTAGC NsiI 2932 ATGCAT ATGCA^T PmaCI 2851 CACGTG CAC^GTG PmlI 2851 CACGTG CAC^GTG RcaI 2840 TCATGA T^CATGA SacII 11 CCGCGG CCGC^GG SapI 490 GAAGAGC GCTCTTC(1/4) ScaI 1938 AGTACT AGT^ACT SexAI 98 ACCAGGT A^CC[AT]GGT SfoI 30 GGCGCC GGC^GCC XbaI 168 TCTAGA T^CTAGA XcmI 534 CCAGGTGATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 12 CGCG CG^CG 736 CGCG AcsI 1614 AAATTT [GA]^AATT[CT] 2326 GAATTC ApaI 87 GGGCCC GGGCC^C 2374 GGGCCC ApoI 1614 AAATTT [GA]^AATT[CT] 2326 GAATTC Asp700I 882 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1311 GAATGGCTTC AspI 64 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1159 GACTCTGTC BsaWI 1086 ACCGGA [AT]^CCGG[AT] 2909 TCCGGA Bsp1407I 417 TGTACA T^GTACA 1466 TGTACA BsrGI 417 TGTACA T^GTACA 1466 TGTACA BstUI 12 CGCG CG^CG 736 CGCG BstYI 555 GGATCC [GA]^GATC[CT] 1422 AGATCT EaeI 2 TGGCCG [CT]^GGCC[GA] 2565 TGGCCA MflI 555 GGATCC [GA]^GATC[CT] 1422 AGATCT MseI 392 TTAA T^TAA 2696 TTAA MvnI 12 CGCG CG^CG 736 CGCG PflFI 64 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1159 GACTCTGTC PflMI 534 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 896 CCAGCCTTTGG SbfI 327 CCTGCAGG CCTGCA^GG 2305 CCTGCAGG SmaI 482 CCCGGG CCC^GGG 1148 CCCGGG SphI 2930 GCATGC GCATG^C 2934 GCATGC Sse8387I 327 CCTGCAGG CCTGCA^GG 2305 CCTGCAGG SspBI 417 TGTACA T^GTACA 1466 TGTACA TaqI 671 TCGA T^CGA 2178 TCGA Tru9I 392 TTAA T^TAA 2696 TTAA Tth111I 64 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1159 GACTCTGTC TthHB8I 671 TCGA T^CGA 2178 TCGA Van91I 534 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 896 CCAGCCTTTGG XhoII 555 GGATCC [GA]^GATC[CT] 1422 AGATCT XmaCI 482 CCCGGG C^CCGGG 1148 CCCGGG XmaI 482 CCCGGG C^CCGGG 1148 CCCGGG XmnI 882 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1311 GAATGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BssSI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TfiI TseI Tsp45I Tsp509I TspRI