Restriction map for pFN21AB0486
 (Linear) (Six-base) MAPSORT of: KIBB0486.seq  Check: 6322  from: 1  to: 3090



KIBB0486.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1126    3090 
   Size:       1126    1964
 
AccI GT'mk_AC

Cuts at:      0     452    3090 
   Size:        452    2638
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     901    1360    1504    1545    2594    2794    3090 
   Size:        316     585     459     144      41    1049     200     296

  Fragments arranged by size: 

                1049     585     459     316     296     200     144      41
            
 
AflIII A'CryG_T

Cuts at:      0    2063    3090 
   Size:       2063    1027
 
AhdI GACnn_n'nnGTC

Cuts at:      0     448    3090 
   Size:        448    2642
 
AleI CACnn'nnGTG

Cuts at:      0    1079    2548    2925    2959    3090 
   Size:       1079    1469     377      34     131

  Fragments arranged by size: 

                1469    1079     377     131      34
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1148    1195    2566    2943    3090 
   Size:       1148      47    1371     377     147

  Fragments arranged by size: 

                1371    1148     377     147      47
 
AlwNI CAG_nnn'CTG

Cuts at:      0     426     843    2319    2453    2512    3090 
   Size:        426     417    1476     134      59     578

  Fragments arranged by size: 

                1476     578     426     417     134      59
 
ApaI G_GGCC'C

Cuts at:      0      91    2378    3090 
   Size:         91    2287     712

  Fragments arranged by size: 

                2287     712      91
 
ApaLI G'TGCA_C

Cuts at:      0      58    3090 
   Size:         58    3032
 
ApoI r'AATT_y

Cuts at:      0    1614    2326    3090 
   Size:       1614     712     764

  Fragments arranged by size: 

                1614     764     712
 
AvaI C'yCGr_G

Cuts at:      0     191     482    1148    2091    3090 
   Size:        191     291     666     943     999

  Fragments arranged by size: 

                 999     943     666     291     191
 
AvrII C'CTAG_G

Cuts at:      0     504    3090 
   Size:        504    2586
 
BamHI G'GATC_C

Cuts at:      0     555    3090 
   Size:        555    2535
 
BanI G'GyrC_C

Cuts at:      0      30      40    1070    1411    3090 
   Size:         30      10    1030     341    1679

  Fragments arranged by size: 

                1679    1030     341      30      10
 
BanII G_rGCy'C

Cuts at:      0      91    1637    1922    2008    2371    2378    3082    3090 
   Size:         91    1546     285      86     363       7     704       8

  Fragments arranged by size: 

                1546     704     363     285      91      86       8       7
            
 
BbeI G_GCGC'C

Cuts at:      0      34    3090 
   Size:         34    3056
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     722     724    1295    2839    3090 
   Size:        722       2     571    1544     251

  Fragments arranged by size: 

                1544     722     571     251       2
 
BbvCI CC'TCA_GC

Cuts at:      0    1009    1022    1589    2195    2800    2884    3090 
   Size:       1009      13     567     606     605      84     206

  Fragments arranged by size: 

                1009     606     605     567     206      84      13
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1554    1588    3090 
   Size:       1554      34    1502

  Fragments arranged by size: 

                1554    1502      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     715    3090 
   Size:        715    2375
 
BfrBI ATG'CAT

Cuts at:      0    2934    3090 
   Size:       2934     156
 
BglI GCCn_nnn'nGGC

Cuts at:      0     372    3090 
   Size:        372    2718
 
BglII A'GATC_T

Cuts at:      0    1422    3090 
   Size:       1422    1668
 
Bme1580I G_kGCm'C

Cuts at:      0      45      62      91     198    1416    2045    2378    3090 
   Size:         45      17      29     107    1218     629     333     712

  Fragments arranged by size: 

                1218     712     629     333     107      45      29      17
            
 
BmtI G_CTAG'C

Cuts at:      0    2341    3090 
   Size:       2341     749
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     736    3090 
   Size:        736    2354
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     588    2776    3090 
   Size:        271     317    2188     314

  Fragments arranged by size: 

                2188     317     314     271
 
Bpu10I CC'TnA_GC

Cuts at:      0    1009    1022    1045    1589    2002    2195    2800    2884 
   Size:       1009      13      23     544     413     193     605      84

Cuts at:   2884    2997    3090 
   Size:        113      93

  Fragments arranged by size: 

                1009     605     544     413     193     113      93      84
                  23      13
 
BsaAI yAC'GTr

Cuts at:      0    2853    3090 
   Size:       2853     237
 
BsaHI Gr'CG_yC

Cuts at:      0      31    3090 
   Size:         31    3059
 
BsaWI w'CCGG_w

Cuts at:      0    1086    2909    3090 
   Size:       1086    1823     181

  Fragments arranged by size: 

                1823    1086     181
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     586     616     625     655    2749    2779    3090 
   Size:        586      30       9      30    2094      30     311

  Fragments arranged by size: 

                2094     586     311      30      30      30       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     649    1606    2112    2281    3090 
   Size:        649     957     506     169     809

  Fragments arranged by size: 

                 957     809     649     506     169
 
BseYI C'CCAG_C

Cuts at:      0     895    1363    1370    1415    1696    3090 
   Size:        895     468       7      45     281    1394

  Fragments arranged by size: 

                1394     895     468     281      45       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1915    2526    2806    3090 
   Size:       1915     611     280     284

  Fragments arranged by size: 

                1915     611     284     280
 
BsiHKAI G_wGCw'C

Cuts at:      0      62    1336    2432    3090 
   Size:         62    1274    1096     658

  Fragments arranged by size: 

                1274    1096     658      62
 
BsmI GAATG_Cn'

Cuts at:      0     852    3090 
   Size:        852    2238
 
Bsp1286I G_dGCh'C

Cuts at:      0      45      62      91     198    1336    1416    1637    1922 
   Size:         45      17      29     107    1138      80     221     285

Cuts at:   1922    2008    2045    2371    2378    2432    3082    3090 
   Size:         86      37     326       7      54     650       8

  Fragments arranged by size: 

                1138     650     326     285     221     107      86      80
                  54      45      37      29      17       8       7
 
BspEI T'CCGG_A

Cuts at:      0    2909    3090 
   Size:       2909     181
 
BspHI T'CATG_A

Cuts at:      0    2840    3090 
   Size:       2840     250
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1126    3090 
   Size:       1126    1964
 
BsrBI CCG'CTC

Cuts at:      0     945    3090 
   Size:        945    2145
 
BsrDI GCAATG_nn'

Cuts at:      0    1179    3090 
   Size:       1179    1911
 
BsrFI r'CCGG_y

Cuts at:      0     290    3090 
   Size:        290    2800
 
BsrGI T'GTAC_A

Cuts at:      0     417    1466    3090 
   Size:        417    1049    1624

  Fragments arranged by size: 

                1624    1049     417
 
BssSI C'ACGA_G

Cuts at:      0      55     197    2558    3090 
   Size:         55     142    2361     532

  Fragments arranged by size: 

                2361     532     142      55
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2931    3090 
   Size:       2931     159
 
BstEII G'GTnAC_C

Cuts at:      0    1459    3090 
   Size:       1459    1631
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3009    3090 
   Size:       3009      81
 
BstYI r'GATC_y

Cuts at:      0     555    1422    3090 
   Size:        555     867    1668

  Fragments arranged by size: 

                1668     867     555
 
Bsu36I CC'TnA_GG

Cuts at:      0    2124    3090 
   Size:       2124     966
 
BtgI C'CryG_G

Cuts at:      0      11     699    1967    2900    3090 
   Size:         11     688    1268     933     190

  Fragments arranged by size: 

                1268     933     688     190      11
 
BtsI GCAGTG_nn'

Cuts at:      0    1460    1714    1985    2146    2920    2932    3050    3090 
   Size:       1460     254     271     161     774      12     118      40

  Fragments arranged by size: 

                1460     774     271     254     161     118      40      12
            
 
ClaI AT'CG_AT

Cuts at:      0     671    3090 
   Size:        671    2419
 
DraIII CAC_nnn'GTG

Cuts at:      0    2040    3090 
   Size:       2040    1050
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2863    3090 
   Size:       2863     227
 
EaeI y'GGCC_r

Cuts at:      0       2    2565    3090 
   Size:          2    2563     525

  Fragments arranged by size: 

                2563     525       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     485    2053    2405    2822    3016    3090 
   Size:        485    1568     352     417     194      74

  Fragments arranged by size: 

                1568     485     417     352     194      74
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1245    2581    3090 
   Size:       1245    1336     509

  Fragments arranged by size: 

                1336    1245     509
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     316     588     901    1360    1504    1545    2594 
   Size:        271      45     272     313     459     144      41    1049

Cuts at:   2594    2776    2794    3090 
   Size:        182      18     296

  Fragments arranged by size: 

                1049     459     313     296     272     271     182     144
                  45      41      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     253     430    1486    3090 
   Size:        253     177    1056    1604

  Fragments arranged by size: 

                1604    1056     253     177
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51      87      88     161     622     960    1026    1833 
   Size:         51      36       1      73     461     338      66     807

Cuts at:   1833    2375    2421    2760    3090 
   Size:        542      46     339     330

  Fragments arranged by size: 

                 807     542     461     339     338     330      73      66
                  51      46      36       1
 
EcoRI G'AATT_C

Cuts at:      0    2326    3090 
   Size:       2326     764
 
FspI TGC'GCA

Cuts at:      0    3034    3090 
   Size:       3034      56
 
HaeII r_GCGC'y

Cuts at:      0      34    3090 
   Size:         34    3056
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     103     135     434     466     586     618    1414    1446 
   Size:        103      32     299      32     120      32     796      32

Cuts at:   1446    1704    1736    2107    2139    2506    2538    2749    2781 
   Size:        258      32     371      32     367      32     211      32

Cuts at:   2781    3090 
   Size:        309

  Fragments arranged by size: 

                 796     371     367     309     299     258     211     120
                 103      32      32      32      32      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0      30    3090 
   Size:         30    3060
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     806    3090 
   Size:        806    2284
 
MscI TGG'CCA

Cuts at:      0    2567    3090 
   Size:       2567     523
 
MslI CAynn'nnrTG

Cuts at:      0     535    1079    2548    2925    2959    3090 
   Size:        535     544    1469     377      34     131

  Fragments arranged by size: 

                1469     544     535     377     131      34
 
MspA1I CmG'CkG

Cuts at:      0       7      13      94    1132    1227    1329    1681    2199 
   Size:          7       6      81    1038      95     102     352     518

Cuts at:   2199    2246    2266    2319    2512    2598    2804    3090 
   Size:         47      20      53     193      86     206     286

  Fragments arranged by size: 

                1038     518     352     286     206     193     102      95
                  86      81      53      47      20       7       6
 
NarI GG'CG_CC

Cuts at:      0      31    3090 
   Size:         31    3059
 
NcoI C'CATG_G

Cuts at:      0     699    1967    2900    3090 
   Size:        699    1268     933     190

  Fragments arranged by size: 

                1268     933     699     190
 
NheI G'CTAG_C

Cuts at:      0    2337    3090 
   Size:       2337     753
 
NsiI A_TGCA'T

Cuts at:      0    2936    3090 
   Size:       2936     154
 
NspI r_CATG'y

Cuts at:      0     418    2934    2938    3090 
   Size:        418    2516       4     152

  Fragments arranged by size: 

                2516     418     152       4
 
PasI CC'CwG_GG

Cuts at:      0     370    3090 
   Size:        370    2720
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     540     902    3090 
   Size:        540     362    2188

  Fragments arranged by size: 

                2188     540     362
 
PfoI T'CCnGG_A

Cuts at:      0     248     404    2111    3090 
   Size:        248     156    1707     979

  Fragments arranged by size: 

                1707     979     248     156
 
PmlI CAC'GTG

Cuts at:      0    2853    3090 
   Size:       2853     237
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2872    2904    3090 
   Size:       2872      32     186

  Fragments arranged by size: 

                2872     186      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     161     622    1026    1833    3090 
   Size:        161     461     404     807    1257

  Fragments arranged by size: 

                1257     807     461     404     161
 
PspOMI G'GGCC_C

Cuts at:      0      87    2374    3090 
   Size:         87    2287     716

  Fragments arranged by size: 

                2287     716      87
 
PstI C_TGCA'G

Cuts at:      0     111     332    1069    1137    1271    1733    2310    2927 
   Size:        111     221     737      68     134     462     577     617

Cuts at:   2927    3090 
   Size:        163

  Fragments arranged by size: 

                 737     617     577     462     221     163     134     111
                  68
 
PvuII CAG'CTG

Cuts at:      0    1132    1329    1681    2199    2246    2319    2512    2804 
   Size:       1132     197     352     518      47      73     193     292

Cuts at:   2804    3090 
   Size:        286

  Fragments arranged by size: 

                1132     518     352     292     286     197     193      73
                  47
 
SacII CC_GC'GG

Cuts at:      0      14    3090 
   Size:         14    3076
 
SanDI GG'GwC_CC

Cuts at:      0     622    3090 
   Size:        622    2468
 
SapI GCTCTTCn'nnn_

Cuts at:      0     485    3090 
   Size:        485    2605
 
SbfI CC_TGCA'GG

Cuts at:      0     332    2310    3090 
   Size:        332    1978     780

  Fragments arranged by size: 

                1978     780     332
 
ScaI AGT'ACT

Cuts at:      0    1940    3090 
   Size:       1940    1150
 
SexAI A'CCwGG_T

Cuts at:      0      98    3090 
   Size:         98    2992
 
SfcI C'TryA_G

Cuts at:      0     107     328     612     835    1057    1065    1133    1267 
   Size:        107     221     284     223     222       8      68     134

Cuts at:   1267    1729    2306    2332    2923    3090 
   Size:        462     577      26     591     167

  Fragments arranged by size: 

                 591     577     462     284     223     222     221     167
                 134     107      68      26       8
 
SfoI GGC'GCC

Cuts at:      0      32    3090 
   Size:         32    3058
 
SmaI CCC'GGG

Cuts at:      0     484    1150    3090 
   Size:        484     666    1940

  Fragments arranged by size: 

                1940     666     484
 
SphI G_CATG'C

Cuts at:      0    2934    2938    3090 
   Size:       2934       4     152

  Fragments arranged by size: 

                2934     152       4
 
SrfI GCCC'GGGC

Cuts at:      0    1150    3090 
   Size:       1150    1940
 
StuI AGG'CCT

Cuts at:      0    1305    1900    2392    3090 
   Size:       1305     595     492     698

  Fragments arranged by size: 

                1305     698     595     492
 
StyI C'CwwG_G

Cuts at:      0     156     504     699    1886    1967    2900    3090 
   Size:        156     348     195    1187      81     933     190

  Fragments arranged by size: 

                1187     933     348     195     190     156      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      28     455    1384    1399    1654    3090 
   Size:         28     427     929      15     255    1436

  Fragments arranged by size: 

                1436     929     427     255      28      15
 
TatI w'GTAC_w

Cuts at:      0     417    1176    1466    1938    2691    2715    3090 
   Size:        417     759     290     472     753      24     375

  Fragments arranged by size: 

                 759     753     472     417     375     290      24
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     188     220     614     646    1315    1347    3090 
   Size:        188      32     394      32     669      32    1743

  Fragments arranged by size: 

                1743     669     394     188      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      67    1162    3090 
   Size:         67    1095    1928

  Fragments arranged by size: 

                1928    1095      67
 
XbaI T'CTAG_A

Cuts at:      0     168    3090 
   Size:        168    2922
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     541    3090 
   Size:        541    2549
 
XmaI C'CCGG_G

Cuts at:      0     482    1148    3090 
   Size:        482     666    1942

  Fragments arranged by size: 

                1942     666     482
 
XmnI GAAnn'nnTTC

Cuts at:      0     886    1315    3090 
   Size:        886     429    1775

  Fragments arranged by size: 

                1775     886     429
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
 Bme1580I     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI   DraIII
     DrdI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
     FspI    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NheI     NsiI     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII    SacII    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SmaI     SphI
     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AloI     AscI
     AseI    AsiSI     BaeI     BclI     BlpI    BmgBI     BmrI    BpuEI
     BsaI    BsaBI    BsiEI    BsiWI    BsmBI   BssHII    BstBI  BstZ17I
    BtgZI    CspCI     DraI     EagI  EcoICRI    EcoRV     FalI     FseI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NaeI
     NdeI   NgoMIV     NotI     NruI     PacI     PciI     PmeI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SspI     SwaI     XhoI
     ZraI
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