(Linear) (Six-base) MAPSORT of: KIBB0486.seq Check: 6322 from: 1 to: 3090 KIBB0486.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1126 3090 Size: 1126 1964 AccI GT'mk_AC Cuts at: 0 452 3090 Size: 452 2638 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 316 901 1360 1504 1545 2594 2794 3090 Size: 316 585 459 144 41 1049 200 296 Fragments arranged by size: 1049 585 459 316 296 200 144 41 AflIII A'CryG_T Cuts at: 0 2063 3090 Size: 2063 1027 AhdI GACnn_n'nnGTC Cuts at: 0 448 3090 Size: 448 2642 AleI CACnn'nnGTG Cuts at: 0 1079 2548 2925 2959 3090 Size: 1079 1469 377 34 131 Fragments arranged by size: 1469 1079 377 131 34 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1148 1195 2566 2943 3090 Size: 1148 47 1371 377 147 Fragments arranged by size: 1371 1148 377 147 47 AlwNI CAG_nnn'CTG Cuts at: 0 426 843 2319 2453 2512 3090 Size: 426 417 1476 134 59 578 Fragments arranged by size: 1476 578 426 417 134 59 ApaI G_GGCC'C Cuts at: 0 91 2378 3090 Size: 91 2287 712 Fragments arranged by size: 2287 712 91 ApaLI G'TGCA_C Cuts at: 0 58 3090 Size: 58 3032 ApoI r'AATT_y Cuts at: 0 1614 2326 3090 Size: 1614 712 764 Fragments arranged by size: 1614 764 712 AvaI C'yCGr_G Cuts at: 0 191 482 1148 2091 3090 Size: 191 291 666 943 999 Fragments arranged by size: 999 943 666 291 191 AvrII C'CTAG_G Cuts at: 0 504 3090 Size: 504 2586 BamHI G'GATC_C Cuts at: 0 555 3090 Size: 555 2535 BanI G'GyrC_C Cuts at: 0 30 40 1070 1411 3090 Size: 30 10 1030 341 1679 Fragments arranged by size: 1679 1030 341 30 10 BanII G_rGCy'C Cuts at: 0 91 1637 1922 2008 2371 2378 3082 3090 Size: 91 1546 285 86 363 7 704 8 Fragments arranged by size: 1546 704 363 285 91 86 8 7 BbeI G_GCGC'C Cuts at: 0 34 3090 Size: 34 3056 BbsI GAAGACnn'nnnn_ Cuts at: 0 722 724 1295 2839 3090 Size: 722 2 571 1544 251 Fragments arranged by size: 1544 722 571 251 2 BbvCI CC'TCA_GC Cuts at: 0 1009 1022 1589 2195 2800 2884 3090 Size: 1009 13 567 606 605 84 206 Fragments arranged by size: 1009 606 605 567 206 84 13 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1554 1588 3090 Size: 1554 34 1502 Fragments arranged by size: 1554 1502 34 BciVI GTATCCnnnnn_n' Cuts at: 0 715 3090 Size: 715 2375 BfrBI ATG'CAT Cuts at: 0 2934 3090 Size: 2934 156 BglI GCCn_nnn'nGGC Cuts at: 0 372 3090 Size: 372 2718 BglII A'GATC_T Cuts at: 0 1422 3090 Size: 1422 1668 Bme1580I G_kGCm'C Cuts at: 0 45 62 91 198 1416 2045 2378 3090 Size: 45 17 29 107 1218 629 333 712 Fragments arranged by size: 1218 712 629 333 107 45 29 17 BmtI G_CTAG'C Cuts at: 0 2341 3090 Size: 2341 749 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 736 3090 Size: 736 2354 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 588 2776 3090 Size: 271 317 2188 314 Fragments arranged by size: 2188 317 314 271 Bpu10I CC'TnA_GC Cuts at: 0 1009 1022 1045 1589 2002 2195 2800 2884 Size: 1009 13 23 544 413 193 605 84 Cuts at: 2884 2997 3090 Size: 113 93 Fragments arranged by size: 1009 605 544 413 193 113 93 84 23 13 BsaAI yAC'GTr Cuts at: 0 2853 3090 Size: 2853 237 BsaHI Gr'CG_yC Cuts at: 0 31 3090 Size: 31 3059 BsaWI w'CCGG_w Cuts at: 0 1086 2909 3090 Size: 1086 1823 181 Fragments arranged by size: 1823 1086 181 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 586 616 625 655 2749 2779 3090 Size: 586 30 9 30 2094 30 311 Fragments arranged by size: 2094 586 311 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 649 1606 2112 2281 3090 Size: 649 957 506 169 809 Fragments arranged by size: 957 809 649 506 169 BseYI C'CCAG_C Cuts at: 0 895 1363 1370 1415 1696 3090 Size: 895 468 7 45 281 1394 Fragments arranged by size: 1394 895 468 281 45 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1915 2526 2806 3090 Size: 1915 611 280 284 Fragments arranged by size: 1915 611 284 280 BsiHKAI G_wGCw'C Cuts at: 0 62 1336 2432 3090 Size: 62 1274 1096 658 Fragments arranged by size: 1274 1096 658 62 BsmI GAATG_Cn' Cuts at: 0 852 3090 Size: 852 2238 Bsp1286I G_dGCh'C Cuts at: 0 45 62 91 198 1336 1416 1637 1922 Size: 45 17 29 107 1138 80 221 285 Cuts at: 1922 2008 2045 2371 2378 2432 3082 3090 Size: 86 37 326 7 54 650 8 Fragments arranged by size: 1138 650 326 285 221 107 86 80 54 45 37 29 17 8 7 BspEI T'CCGG_A Cuts at: 0 2909 3090 Size: 2909 181 BspHI T'CATG_A Cuts at: 0 2840 3090 Size: 2840 250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1126 3090 Size: 1126 1964 BsrBI CCG'CTC Cuts at: 0 945 3090 Size: 945 2145 BsrDI GCAATG_nn' Cuts at: 0 1179 3090 Size: 1179 1911 BsrFI r'CCGG_y Cuts at: 0 290 3090 Size: 290 2800 BsrGI T'GTAC_A Cuts at: 0 417 1466 3090 Size: 417 1049 1624 Fragments arranged by size: 1624 1049 417 BssSI C'ACGA_G Cuts at: 0 55 197 2558 3090 Size: 55 142 2361 532 Fragments arranged by size: 2361 532 142 55 BstAPI GCAn_nnn'nTGC Cuts at: 0 2931 3090 Size: 2931 159 BstEII G'GTnAC_C Cuts at: 0 1459 3090 Size: 1459 1631 BstXI CCAn_nnnn'nTGG Cuts at: 0 3009 3090 Size: 3009 81 BstYI r'GATC_y Cuts at: 0 555 1422 3090 Size: 555 867 1668 Fragments arranged by size: 1668 867 555 Bsu36I CC'TnA_GG Cuts at: 0 2124 3090 Size: 2124 966 BtgI C'CryG_G Cuts at: 0 11 699 1967 2900 3090 Size: 11 688 1268 933 190 Fragments arranged by size: 1268 933 688 190 11 BtsI GCAGTG_nn' Cuts at: 0 1460 1714 1985 2146 2920 2932 3050 3090 Size: 1460 254 271 161 774 12 118 40 Fragments arranged by size: 1460 774 271 254 161 118 40 12 ClaI AT'CG_AT Cuts at: 0 671 3090 Size: 671 2419 DraIII CAC_nnn'GTG Cuts at: 0 2040 3090 Size: 2040 1050 DrdI GACnn_nn'nnGTC Cuts at: 0 2863 3090 Size: 2863 227 EaeI y'GGCC_r Cuts at: 0 2 2565 3090 Size: 2 2563 525 Fragments arranged by size: 2563 525 2 EarI CTCTTCn'nnn_ Cuts at: 0 485 2053 2405 2822 3016 3090 Size: 485 1568 352 417 194 74 Fragments arranged by size: 1568 485 417 352 194 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1245 2581 3090 Size: 1245 1336 509 Fragments arranged by size: 1336 1245 509 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 316 588 901 1360 1504 1545 2594 Size: 271 45 272 313 459 144 41 1049 Cuts at: 2594 2776 2794 3090 Size: 182 18 296 Fragments arranged by size: 1049 459 313 296 272 271 182 144 45 41 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 253 430 1486 3090 Size: 253 177 1056 1604 Fragments arranged by size: 1604 1056 253 177 EcoO109I rG'GnC_Cy Cuts at: 0 51 87 88 161 622 960 1026 1833 Size: 51 36 1 73 461 338 66 807 Cuts at: 1833 2375 2421 2760 3090 Size: 542 46 339 330 Fragments arranged by size: 807 542 461 339 338 330 73 66 51 46 36 1 EcoRI G'AATT_C Cuts at: 0 2326 3090 Size: 2326 764 FspI TGC'GCA Cuts at: 0 3034 3090 Size: 3034 56 HaeII r_GCGC'y Cuts at: 0 34 3090 Size: 34 3056 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 103 135 434 466 586 618 1414 1446 Size: 103 32 299 32 120 32 796 32 Cuts at: 1446 1704 1736 2107 2139 2506 2538 2749 2781 Size: 258 32 371 32 367 32 211 32 Cuts at: 2781 3090 Size: 309 Fragments arranged by size: 796 371 367 309 299 258 211 120 103 32 32 32 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 30 3090 Size: 30 3060 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 806 3090 Size: 806 2284 MscI TGG'CCA Cuts at: 0 2567 3090 Size: 2567 523 MslI CAynn'nnrTG Cuts at: 0 535 1079 2548 2925 2959 3090 Size: 535 544 1469 377 34 131 Fragments arranged by size: 1469 544 535 377 131 34 MspA1I CmG'CkG Cuts at: 0 7 13 94 1132 1227 1329 1681 2199 Size: 7 6 81 1038 95 102 352 518 Cuts at: 2199 2246 2266 2319 2512 2598 2804 3090 Size: 47 20 53 193 86 206 286 Fragments arranged by size: 1038 518 352 286 206 193 102 95 86 81 53 47 20 7 6 NarI GG'CG_CC Cuts at: 0 31 3090 Size: 31 3059 NcoI C'CATG_G Cuts at: 0 699 1967 2900 3090 Size: 699 1268 933 190 Fragments arranged by size: 1268 933 699 190 NheI G'CTAG_C Cuts at: 0 2337 3090 Size: 2337 753 NsiI A_TGCA'T Cuts at: 0 2936 3090 Size: 2936 154 NspI r_CATG'y Cuts at: 0 418 2934 2938 3090 Size: 418 2516 4 152 Fragments arranged by size: 2516 418 152 4 PasI CC'CwG_GG Cuts at: 0 370 3090 Size: 370 2720 PflMI CCAn_nnn'nTGG Cuts at: 0 540 902 3090 Size: 540 362 2188 Fragments arranged by size: 2188 540 362 PfoI T'CCnGG_A Cuts at: 0 248 404 2111 3090 Size: 248 156 1707 979 Fragments arranged by size: 1707 979 248 156 PmlI CAC'GTG Cuts at: 0 2853 3090 Size: 2853 237 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2872 2904 3090 Size: 2872 32 186 Fragments arranged by size: 2872 186 32 PpuMI rG'GwC_Cy Cuts at: 0 161 622 1026 1833 3090 Size: 161 461 404 807 1257 Fragments arranged by size: 1257 807 461 404 161 PspOMI G'GGCC_C Cuts at: 0 87 2374 3090 Size: 87 2287 716 Fragments arranged by size: 2287 716 87 PstI C_TGCA'G Cuts at: 0 111 332 1069 1137 1271 1733 2310 2927 Size: 111 221 737 68 134 462 577 617 Cuts at: 2927 3090 Size: 163 Fragments arranged by size: 737 617 577 462 221 163 134 111 68 PvuII CAG'CTG Cuts at: 0 1132 1329 1681 2199 2246 2319 2512 2804 Size: 1132 197 352 518 47 73 193 292 Cuts at: 2804 3090 Size: 286 Fragments arranged by size: 1132 518 352 292 286 197 193 73 47 SacII CC_GC'GG Cuts at: 0 14 3090 Size: 14 3076 SanDI GG'GwC_CC Cuts at: 0 622 3090 Size: 622 2468 SapI GCTCTTCn'nnn_ Cuts at: 0 485 3090 Size: 485 2605 SbfI CC_TGCA'GG Cuts at: 0 332 2310 3090 Size: 332 1978 780 Fragments arranged by size: 1978 780 332 ScaI AGT'ACT Cuts at: 0 1940 3090 Size: 1940 1150 SexAI A'CCwGG_T Cuts at: 0 98 3090 Size: 98 2992 SfcI C'TryA_G Cuts at: 0 107 328 612 835 1057 1065 1133 1267 Size: 107 221 284 223 222 8 68 134 Cuts at: 1267 1729 2306 2332 2923 3090 Size: 462 577 26 591 167 Fragments arranged by size: 591 577 462 284 223 222 221 167 134 107 68 26 8 SfoI GGC'GCC Cuts at: 0 32 3090 Size: 32 3058 SmaI CCC'GGG Cuts at: 0 484 1150 3090 Size: 484 666 1940 Fragments arranged by size: 1940 666 484 SphI G_CATG'C Cuts at: 0 2934 2938 3090 Size: 2934 4 152 Fragments arranged by size: 2934 152 4 SrfI GCCC'GGGC Cuts at: 0 1150 3090 Size: 1150 1940 StuI AGG'CCT Cuts at: 0 1305 1900 2392 3090 Size: 1305 595 492 698 Fragments arranged by size: 1305 698 595 492 StyI C'CwwG_G Cuts at: 0 156 504 699 1886 1967 2900 3090 Size: 156 348 195 1187 81 933 190 Fragments arranged by size: 1187 933 348 195 190 156 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 28 455 1384 1399 1654 3090 Size: 28 427 929 15 255 1436 Fragments arranged by size: 1436 929 427 255 28 15 TatI w'GTAC_w Cuts at: 0 417 1176 1466 1938 2691 2715 3090 Size: 417 759 290 472 753 24 375 Fragments arranged by size: 759 753 472 417 375 290 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 188 220 614 646 1315 1347 3090 Size: 188 32 394 32 669 32 1743 Fragments arranged by size: 1743 669 394 188 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 67 1162 3090 Size: 67 1095 1928 Fragments arranged by size: 1928 1095 67 XbaI T'CTAG_A Cuts at: 0 168 3090 Size: 168 2922 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 541 3090 Size: 541 2549 XmaI C'CCGG_G Cuts at: 0 482 1148 3090 Size: 482 666 1942 Fragments arranged by size: 1942 666 482 XmnI GAAnn'nnTTC Cuts at: 0 886 1315 3090 Size: 886 429 1775 Fragments arranged by size: 1775 886 429 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmtI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI NheI NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacII SanDI SapI SbfI ScaI SexAI SfcI SfoI SmaI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AloI AscI AseI AsiSI BaeI BclI BlpI BmgBI BmrI BpuEI BsaI BsaBI BsiEI BsiWI BsmBI BssHII BstBI BstZ17I BtgZI CspCI DraI EagI EcoICRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NotI NruI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SfiI SgfI SgrAI SmlI SnaBI SpeI SspI SwaI XhoI ZraI