################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AscI Asp718I AspEI AspI AvrII BalI BamHI BbrPI BbvCI BcgI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BspMI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I EarI EcoNI EcoO65I EcoRI EcoT22I FbaI FseI HpaI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Alw44I 1516 GTGCAC G^TGCAC AlwNI 1232 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 264 CCTGAGG CC^T[ACGT]AGG Aor51HI 21 AGCGCT AGC^GCT ApaLI 1516 GTGCAC G^TGCAC AseI 745 ATTAAT AT^TAAT AsnI 745 ATTAAT AT^TAAT Asp700I 1632 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1565 TGCGCA TGC^GCA BciVI 446 GTATCC GTATCC(6/5) BfaI 625 CTAG C^TAG BglI 70 GCCGACTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 515 GTGCAG GTGCAG(16/14) BsmAI 350 GTCTC GTCTC(1/5) BsmI 1290 GAATGC GAATGC(1/-1) Bsp1407I 1646 TGTACA T^GTACA BsrBI 688 GAGCGG CCGCTC(-3/-3) BsrDI 1200 CATTGC GCAATG(2/0) BsrGI 1646 TGTACA T^GTACA BstXI 233 CCAGCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 264 CCTGAGG CC^T[ACGT]AGG EagI 77 CGGCCG C^GGCCG EclXI 77 CGGCCG C^GGCCG Eco47III 21 AGCGCT AGC^GCT Eco52I 77 CGGCCG C^GGCCG Eco81I 264 CCTGAGG CC^T[ACGT]AGG EcoRV 1488 GATATC GAT^ATC FspI 1565 TGCGCA TGC^GCA HincII 1161 GTTGAC GT[CT]^[GA]AC HindII 1161 GTTGAC GT[CT]^[GA]AC MaeI 625 CTAG C^TAG NheI 624 GCTAGC G^CTAGC PflMI 889 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 745 ATTAAT AT^TAAT PvuI 222 CGATCG CGAT^CG SfcI 156 CTACAG C^T[GA][CT]AG SspBI 1646 TGTACA T^GTACA Van91I 889 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1632 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 843 AAATTT [GA]^AATT[CT] 1429 AAATTT ApoI 843 AAATTT [GA]^AATT[CT] 1429 AAATTT AspHI 678 GAGCAC G[AT]GC[AT]^C 1516 GTGCAC BbeI 149 GGCGCC GGCGC^C 229 GGCGCC BglII 1244 AGATCT A^GATCT 1278 AGATCT BseRI 283 GAGGAG GAGGAG(10/8) 1298 GAGGAG BsiHKAI 678 GAGCAC G[AT]GC[AT]^C 1516 GTGCAC BsmFI 102 GGGAC GGGAC(10/14) 33 GTCCC BstAPI 428 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1557 GCAAATTATGC BstF5I 312 CATCC GGATG(2/0) 363 CATCC BstYI 1244 AGATCT [GA]^GATC[CT] 1278 AGATCT DraII 260 AGGACCT [GA]G^G[ACGT]CC[CT] 268 AGGACCC EcoO109I 260 AGGACCT [GA]G^G[ACGT]CC[CT] 268 AGGACCC FokI 312 CATCC GGATG(9/13) 363 CATCC HgaI 800 GACGC GACGC(5/10) 64 GCGTC HindIII 485 AAGCTT A^AGCTT 857 AAGCTT KasI 149 GGCGCC G^GCGCC 229 GGCGCC MflI 1244 AGATCT [GA]^GATC[CT] 1278 AGATCT NarI 149 GGCGCC GG^CGCC 229 GGCGCC PpuMI 260 AGGACCT [GA]G^G[AT]CC[CT] 268 AGGACCC PvuII 1142 CAGCTG CAG^CTG 1607 CAGCTG SfoI 149 GGCGCC GGC^GCC 229 GGCGCC TaqI 221 TCGA T^CGA 395 TCGA TthHB8I 221 TCGA T^CGA 395 TCGA XhoII 1244 AGATCT [GA]^GATC[CT] 1278 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AvaI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BsiEI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmaCI XmaI