(Circular) (Six-base) MAPSORT of: pFN21AE1982.seq Check: 5404 from: 1 to: 6379 pFN21AE1982 6379 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4189 278 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3722 1937 Size: 1785 4594 Acc65I G'GTAC_C Cuts at: 3700 3700 Size: 6379 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3231 3353 4442 5826 6094 1632 Size: 1599 122 1089 1384 268 1917 Fragments arranged by size: 1917 1599 1384 1089 268 122 AfeI AGC'GCT Cuts at: 2028 2028 Size: 6379 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6182 AflIII A'CryG_T Cuts at: 1203 5278 6255 1203 Size: 4075 977 1327 Fragments arranged by size: 4075 1327 977 AgeI A'CCGG_T Cuts at: 5210 5210 Size: 6379 AhdI GACnn_n'nnGTC Cuts at: 5108 5108 Size: 6379 AleI CACnn'nnGTG Cuts at: 1166 2468 2763 1166 Size: 1302 295 4782 Fragments arranged by size: 4782 1302 295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2363 2681 1184 Size: 1179 318 4882 Fragments arranged by size: 4882 1179 318 AlwNI CAG_nnn'CTG Cuts at: 3242 4122 5694 3242 Size: 880 1572 3927 Fragments arranged by size: 3927 1572 880 ApaI G_GGCC'C Cuts at: 2052 2104 2416 2052 Size: 52 312 6015 Fragments arranged by size: 6015 312 52 ApaLI G'TGCA_C Cuts at: 3521 4436 5592 3521 Size: 915 1156 4308 Fragments arranged by size: 4308 1156 915 ApoI r'AATT_y Cuts at: 1493 2848 3434 3688 3914 4066 6175 1493 Size: 1355 586 254 226 152 2109 1697 Fragments arranged by size: 2109 1697 1355 586 254 226 152 AseI AT'TA_AT Cuts at: 160 1032 2751 4933 160 Size: 872 1719 2182 1606 Fragments arranged by size: 2182 1719 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 2040 2103 2277 3704 1870 Size: 73 97 63 174 1427 4545 Fragments arranged by size: 4545 1427 174 97 73 63 BamHI G'GATC_C Cuts at: 3709 4076 3709 Size: 367 6012 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2154 2234 3215 3700 5155 Size: 325 217 627 367 80 981 485 1455 Cuts at: 5155 618 Size: 1842 Fragments arranged by size: 1842 1455 981 627 485 367 325 217 80 BanII G_rGCy'C Cuts at: 1391 2052 2066 2104 2416 3698 1391 Size: 661 14 38 312 1282 4072 Fragments arranged by size: 4072 1282 661 312 38 14 BbeI G_GCGC'C Cuts at: 2158 2238 2158 Size: 80 6299 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2824 3057 3634 928 Size: 971 925 233 577 3673 Fragments arranged by size: 3673 971 925 577 233 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4570 4604 1903 Size: 34 2633 34 3678 Fragments arranged by size: 3678 2633 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2462 4271 5481 2462 Size: 1809 1210 3360 Fragments arranged by size: 3360 1809 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6379 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2081 4990 136 Size: 107 122 71 631 605 409 2909 1525 Fragments arranged by size: 2909 1525 631 605 409 122 107 71 BglII A'GATC_T Cuts at: 3249 3283 6374 3249 Size: 34 3091 3254 Fragments arranged by size: 3254 3091 34 BlpI GC'TnA_GC Cuts at: 3796 3796 Size: 6379 Bme1580I G_kGCm'C Cuts at: 1790 2052 2104 2416 2742 3525 4440 5596 1790 Size: 262 52 312 326 783 915 1156 2573 Fragments arranged by size: 2573 1156 915 783 326 312 262 52 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4110 5068 442 Size: 416 53 83 399 24 2693 958 1753 Fragments arranged by size: 2693 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2633 1056 Size: 1577 4802 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2658 3219 3278 3321 5039 1239 Size: 1419 561 59 43 1718 2579 Fragments arranged by size: 2579 1718 1419 561 59 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4504 5369 5667 4504 Size: 865 298 5216 Fragments arranged by size: 5216 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5042 882 Size: 617 3543 2219 Fragments arranged by size: 3543 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4170 493 Size: 711 2966 2702 Fragments arranged by size: 2966 2702 711 BsaBI GATnn'nnATC Cuts at: 6373 6373 Size: 6379 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 2155 2235 4186 Size: 53 83 186 936 536 86 80 1951 Cuts at: 4186 4568 5203 275 Size: 382 635 1451 Fragments arranged by size: 1951 1451 936 635 536 382 186 86 83 80 53 BsaWI w'CCGG_w Cuts at: 4812 5210 5484 5631 6042 4812 Size: 398 274 147 411 5149 Fragments arranged by size: 5149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2369 2399 3297 3327 1178 Size: 30 165 30 966 30 898 30 4230 Fragments arranged by size: 4230 966 898 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 3318 1186 Size: 1117 1015 4247 Fragments arranged by size: 4247 1117 1015 BseYI C'CCAG_C Cuts at: 2092 2097 2415 3145 5582 6078 2092 Size: 5 318 730 2437 496 2393 Fragments arranged by size: 2437 2393 730 496 318 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2541 1163 Size: 573 805 5001 Fragments arranged by size: 5001 805 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2085 2182 2230 3829 4590 5618 Size: 275 186 84 97 48 1599 761 1028 Cuts at: 5618 1540 Size: 2301 Fragments arranged by size: 2301 1599 1028 761 275 186 97 84 48 BsiHKAI G_wGCw'C Cuts at: 2687 3525 4440 4525 5596 2687 Size: 838 915 85 1071 3470 Fragments arranged by size: 3470 1071 915 838 85 BsmI GAATG_Cn' Cuts at: 3301 3890 3983 3301 Size: 589 93 5697 Fragments arranged by size: 5697 589 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2066 2104 2416 2687 2742 3525 Size: 399 262 14 38 312 271 55 783 Cuts at: 3525 3698 4440 4525 5596 1391 Size: 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 399 312 271 262 173 85 55 38 14 BspEI T'CCGG_A Cuts at: 6042 6042 Size: 6379 BspHI T'CATG_A Cuts at: 4268 5998 4268 Size: 1730 4649 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3734 844 Size: 2890 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2695 4266 1394 Size: 429 872 1571 3507 Fragments arranged by size: 3507 1571 872 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3204 4874 5048 65 Size: 1183 1956 1670 174 1396 Fragments arranged by size: 1956 1670 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5023 5210 1700 Size: 110 3213 187 2869 Fragments arranged by size: 3213 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3651 96 Size: 3555 2824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 4439 5451 4439 Size: 1012 5367 BstAPI GCAn_nnn'nTGC Cuts at: 794 2439 3568 794 Size: 1645 1129 3605 Fragments arranged by size: 3605 1645 1129 BstBI TT'CG_AA Cuts at: 5183 5183 Size: 6379 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6379 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2245 Size: 6379 BstYI r'GATC_y Cuts at: 1972 3249 3283 3709 4076 4461 4478 5930 6016 Size: 1277 34 426 367 385 17 1452 86 Cuts at: 6016 6028 6374 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1277 426 385 367 346 86 34 17 12 Bsu36I CC'TnA_GG Cuts at: 2270 2270 Size: 6379 BtgI C'CryG_G Cuts at: 513 1065 2622 2800 2824 2836 3471 513 Size: 552 1557 178 24 12 635 3421 Fragments arranged by size: 3421 1557 635 552 178 24 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5640 Fragments arranged by size: 5640 683 56 BtsI GCAGTG_nn' Cuts at: 2529 3895 4689 4709 2529 Size: 1366 794 20 4199 Fragments arranged by size: 4199 1366 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6344 DraI TTT'AAA Cuts at: 3683 3683 Size: 6379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5386 809 Size: 507 4070 1802 Fragments arranged by size: 4070 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 2145 2175 Size: 54 1425 50 275 18 252 63 30 Cuts at: 2175 2821 3358 3826 4715 6344 8 Size: 646 537 468 889 1629 43 Fragments arranged by size: 1629 1425 889 646 537 468 275 252 63 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2082 3826 1537 Size: 275 270 1744 4090 Fragments arranged by size: 4090 1744 275 270 EarI CTCTTCn'nnn_ Cuts at: 1353 4309 1353 Size: 2956 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2062 4966 5340 5486 387 Size: 1064 138 473 2904 374 146 1280 Fragments arranged by size: 2904 1280 1064 473 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2658 3219 3231 3278 3321 3353 4442 Size: 393 1026 561 12 47 43 32 1089 Cuts at: 4442 5039 5826 6094 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1089 1026 787 597 561 393 268 47 43 32 12 EcoO109I rG'GnC_Cy Cuts at: 2266 2274 2266 Size: 8 6371 EcoRI G'AATT_C Cuts at: 3688 4066 3688 Size: 378 6001 EcoRV GAT'ATC Cuts at: 3495 3495 Size: 6379 FspI TGC'GCA Cuts at: 3572 4885 3572 Size: 1313 5066 FspAI rTGC'GCAy Cuts at: 3572 3572 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 2030 2158 2238 5526 1678 Size: 352 128 80 3288 2531 Fragments arranged by size: 3288 2531 352 128 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2369 2401 3479 3511 5020 Size: 32 135 32 797 32 1078 32 1509 Cuts at: 5020 5052 5094 5126 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1509 1078 797 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3723 3969 669 Size: 988 281 1230 555 246 3079 Fragments arranged by size: 3079 1230 988 555 281 246 HindIII A'AGCT_T Cuts at: 748 2490 2862 748 Size: 1742 372 4265 Fragments arranged by size: 4265 1742 372 HpaI GTT'AAC Cuts at: 3969 3969 Size: 6379 KasI G'GCGC_C Cuts at: 2154 2234 2154 Size: 80 6299 KpnI G_GTAC'C Cuts at: 3704 3704 Size: 6379 MfeI C'AATT_G Cuts at: 3978 3978 Size: 6379 MluI A'CGCG_T Cuts at: 6255 6255 Size: 6379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3201 5493 5677 1305 Size: 1896 2292 184 2007 Fragments arranged by size: 2292 2007 1896 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4557 Fragments arranged by size: 4557 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2468 2515 2663 2763 3578 4337 4696 Size: 648 1302 47 148 100 815 759 359 Cuts at: 4696 4855 518 Size: 159 2042 Fragments arranged by size: 2042 1302 815 759 648 359 159 148 100 47 MspA1I CmG'CkG Cuts at: 1606 2054 3149 3614 3795 4472 5620 5865 1606 Size: 448 1095 465 181 677 1148 245 2120 Fragments arranged by size: 2120 1148 1095 677 465 448 245 181 NarI GG'CG_CC Cuts at: 2155 2235 2155 Size: 80 6299 NcoI C'CATG_G Cuts at: 513 1065 2622 2800 2836 513 Size: 552 1557 178 36 4056 Fragments arranged by size: 4056 1557 552 178 36 NdeI CA'TA_TG Cuts at: 387 387 Size: 6379 NheI G'CTAG_C Cuts at: 1052 2629 1052 Size: 1577 4802 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NspI r_CATG'y Cuts at: 1132 1229 3737 5282 1132 Size: 97 2508 1545 2229 Fragments arranged by size: 2508 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2059 1348 Size: 711 5668 PciI A'CATG_T Cuts at: 5278 5278 Size: 6379 PflMI CCAn_nnn'nTGG Cuts at: 1108 2900 1108 Size: 1792 4587 PfoI T'CCnGG_A Cuts at: 2705 3255 2705 Size: 550 5829 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4448 4480 5961 5993 4448 Size: 32 1481 32 4834 Fragments arranged by size: 4834 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2266 2274 2266 Size: 8 6371 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6379 PsiI TTA'TAA Cuts at: 3949 3949 Size: 6379 PspOMI G'GGCC_C Cuts at: 2048 2100 2412 2048 Size: 52 312 6015 Fragments arranged by size: 6015 312 52 PstI C_TGCA'G Cuts at: 830 3731 830 Size: 2901 3478 PvuI CG_AT'CG Cuts at: 2001 2230 2001 Size: 229 6150 PvuII CAG'CTG Cuts at: 3149 3614 3149 Size: 465 5914 SalI G'TCGA_C Cuts at: 1936 3721 1936 Size: 1785 4594 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 4627 6339 4627 Size: 1712 4667 SfcI C'TryA_G Cuts at: 826 1045 2161 3727 4862 5543 5734 826 Size: 219 1116 1566 1135 681 191 1471 Fragments arranged by size: 1566 1471 1135 1116 681 219 191 SfoI GGC'GCC Cuts at: 2156 2236 2156 Size: 80 6299 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 2042 2105 2279 3706 1872 Size: 170 63 174 1427 4545 Fragments arranged by size: 4545 1427 174 170 63 SmlI C'TyrA_G Cuts at: 820 1017 1943 4483 5384 5646 820 Size: 197 926 2540 901 262 1553 Fragments arranged by size: 2540 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4170 493 Size: 3677 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6379 SphI G_CATG'C Cuts at: 1132 3737 1132 Size: 2605 3774 SspI AAT'ATT Cuts at: 5 52 4303 5 Size: 47 4251 2081 Fragments arranged by size: 4251 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6379 StyI C'CwwG_G Cuts at: 513 1065 2622 2788 2800 2836 3090 3818 513 Size: 552 1557 166 12 36 254 728 3074 Fragments arranged by size: 3074 1557 728 552 254 166 36 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3199 4406 4423 4576 4761 5206 325 Size: 1255 1619 1207 17 153 185 445 1498 Fragments arranged by size: 1619 1498 1255 1207 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3651 4625 6337 Size: 274 80 33 51 1443 1674 974 1712 Cuts at: 6337 96 Size: 138 Fragments arranged by size: 1712 1674 1443 974 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3484 3516 6339 6371 3484 Size: 32 2823 32 3492 Fragments arranged by size: 3492 2823 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6379 XbaI T'CTAG_A Cuts at: 3715 3715 Size: 6379 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6379 XmaI C'CCGG_G Cuts at: 1870 2040 2103 2277 3704 1870 Size: 170 63 174 1427 4545 Fragments arranged by size: 4545 1427 174 170 63 XmnI GAAnn'nnTTC Cuts at: 3641 4508 3641 Size: 867 5512 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4187 276 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI