(Linear) (Six-base) MAPSORT of: KIEE1982.seq Check: 3744 from: 1 to: 1674 KIEE1982.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1226 1348 1674 Size: 1226 122 326 Fragments arranged by size: 1226 326 122 AfeI AGC'GCT Cuts at: 0 23 1674 Size: 23 1651 AleI CACnn'nnGTG Cuts at: 0 463 758 1674 Size: 463 295 916 Fragments arranged by size: 916 463 295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 358 676 1674 Size: 358 318 998 Fragments arranged by size: 998 358 318 AlwNI CAG_nnn'CTG Cuts at: 0 1237 1674 Size: 1237 437 ApaI G_GGCC'C Cuts at: 0 47 99 411 1674 Size: 47 52 312 1263 Fragments arranged by size: 1263 312 52 47 ApaLI G'TGCA_C Cuts at: 0 1516 1674 Size: 1516 158 ApoI r'AATT_y Cuts at: 0 843 1429 1674 Size: 843 586 245 Fragments arranged by size: 843 586 245 AseI AT'TA_AT Cuts at: 0 746 1674 Size: 746 928 AvaI C'yCGr_G Cuts at: 0 35 98 272 1674 Size: 35 63 174 1402 Fragments arranged by size: 1402 174 63 35 BanI G'GyrC_C Cuts at: 0 149 229 1210 1674 Size: 149 80 981 464 Fragments arranged by size: 981 464 149 80 BanII G_rGCy'C Cuts at: 0 47 61 99 411 1674 Size: 47 14 38 312 1263 Fragments arranged by size: 1263 312 47 38 14 BbeI G_GCGC'C Cuts at: 0 153 233 1674 Size: 153 80 1441 Fragments arranged by size: 1441 153 80 BbsI GAAGACnn'nnnn_ Cuts at: 0 819 1052 1629 1674 Size: 819 233 577 45 Fragments arranged by size: 819 577 233 45 BciVI GTATCCnnnnn_n' Cuts at: 0 457 1674 Size: 457 1217 BglI GCCn_nnn'nGGC Cuts at: 0 76 1674 Size: 76 1598 BglII A'GATC_T Cuts at: 0 1244 1278 1674 Size: 1244 34 396 Fragments arranged by size: 1244 396 34 Bme1580I G_kGCm'C Cuts at: 0 47 99 411 737 1520 1674 Size: 47 52 312 326 783 154 Fragments arranged by size: 783 326 312 154 52 47 BmtI G_CTAG'C Cuts at: 0 628 1674 Size: 628 1046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 653 1214 1273 1316 1674 Size: 653 561 59 43 358 Fragments arranged by size: 653 561 358 59 43 BsaHI Gr'CG_yC Cuts at: 0 64 150 230 1674 Size: 64 86 80 1444 Fragments arranged by size: 1444 86 80 64 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 364 394 1292 1322 1674 Size: 364 30 898 30 352 Fragments arranged by size: 898 364 352 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 298 1313 1674 Size: 298 1015 361 Fragments arranged by size: 1015 361 298 BseYI C'CCAG_C Cuts at: 0 87 92 410 1140 1674 Size: 87 5 318 730 534 Fragments arranged by size: 730 534 318 87 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 536 1674 Size: 536 1138 BsiEI CG_ry'CG Cuts at: 0 80 177 225 1674 Size: 80 97 48 1449 Fragments arranged by size: 1449 97 80 48 BsiHKAI G_wGCw'C Cuts at: 0 682 1520 1674 Size: 682 838 154 Fragments arranged by size: 838 682 154 BsmI GAATG_Cn' Cuts at: 0 1296 1674 Size: 1296 378 Bsp1286I G_dGCh'C Cuts at: 0 47 61 99 411 682 737 1520 1674 Size: 47 14 38 312 271 55 783 154 Fragments arranged by size: 783 312 271 154 55 47 38 14 BsrBI CCG'CTC Cuts at: 0 690 1674 Size: 690 984 BsrDI GCAATG_nn' Cuts at: 0 1199 1674 Size: 1199 475 BsrGI T'GTAC_A Cuts at: 0 1646 1674 Size: 1646 28 BstAPI GCAn_nnn'nTGC Cuts at: 0 434 1563 1674 Size: 434 1129 111 Fragments arranged by size: 1129 434 111 BstXI CCAn_nnnn'nTGG Cuts at: 0 240 1674 Size: 240 1434 BstYI r'GATC_y Cuts at: 0 1244 1278 1674 Size: 1244 34 396 Fragments arranged by size: 1244 396 34 Bsu36I CC'TnA_GG Cuts at: 0 265 1674 Size: 265 1409 BtgI C'CryG_G Cuts at: 0 617 795 819 831 1466 1674 Size: 617 178 24 12 635 208 Fragments arranged by size: 635 617 208 178 24 12 BtsI GCAGTG_nn' Cuts at: 0 524 1674 Size: 524 1150 EaeI y'GGCC_r Cuts at: 0 77 140 170 816 1353 1674 Size: 77 63 30 646 537 321 Fragments arranged by size: 646 537 321 77 63 30 EagI C'GGCC_G Cuts at: 0 77 1674 Size: 77 1597 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 57 1674 Size: 57 1617 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 653 1214 1226 1273 1316 1348 1674 Size: 653 561 12 47 43 32 326 Fragments arranged by size: 653 561 326 47 43 32 12 EcoO109I rG'GnC_Cy Cuts at: 0 261 269 1674 Size: 261 8 1405 Fragments arranged by size: 1405 261 8 EcoRV GAT'ATC Cuts at: 0 1490 1674 Size: 1490 184 FspI TGC'GCA Cuts at: 0 1567 1674 Size: 1567 107 FspAI rTGC'GCAy Cuts at: 0 1567 1674 Size: 1567 107 HaeII r_GCGC'y Cuts at: 0 25 153 233 1674 Size: 25 128 80 1441 Fragments arranged by size: 1441 128 80 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 364 396 1474 1506 1674 Size: 364 32 1078 32 168 Fragments arranged by size: 1078 364 168 32 32 HincII GTy'rAC Cuts at: 0 1163 1674 Size: 1163 511 HindIII A'AGCT_T Cuts at: 0 485 857 1674 Size: 485 372 817 Fragments arranged by size: 817 485 372 KasI G'GCGC_C Cuts at: 0 149 229 1674 Size: 149 80 1445 Fragments arranged by size: 1445 149 80 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1196 1674 Size: 1196 478 MslI CAynn'nnrTG Cuts at: 0 463 510 658 758 1573 1674 Size: 463 47 148 100 815 101 Fragments arranged by size: 815 463 148 101 100 47 MspA1I CmG'CkG Cuts at: 0 49 1144 1609 1674 Size: 49 1095 465 65 Fragments arranged by size: 1095 465 65 49 NarI GG'CG_CC Cuts at: 0 150 230 1674 Size: 150 80 1444 Fragments arranged by size: 1444 150 80 NcoI C'CATG_G Cuts at: 0 617 795 831 1674 Size: 617 178 36 843 Fragments arranged by size: 843 617 178 36 NheI G'CTAG_C Cuts at: 0 624 1674 Size: 624 1050 PasI CC'CwG_GG Cuts at: 0 54 1674 Size: 54 1620 PflMI CCAn_nnn'nTGG Cuts at: 0 895 1674 Size: 895 779 PfoI T'CCnGG_A Cuts at: 0 700 1250 1674 Size: 700 550 424 Fragments arranged by size: 700 550 424 PpuMI rG'GwC_Cy Cuts at: 0 261 269 1674 Size: 261 8 1405 Fragments arranged by size: 1405 261 8 PspOMI G'GGCC_C Cuts at: 0 43 95 407 1674 Size: 43 52 312 1267 Fragments arranged by size: 1267 312 52 43 PvuI CG_AT'CG Cuts at: 0 225 1674 Size: 225 1449 PvuII CAG'CTG Cuts at: 0 1144 1609 1674 Size: 1144 465 65 Fragments arranged by size: 1144 465 65 SfcI C'TryA_G Cuts at: 0 156 1674 Size: 156 1518 SfoI GGC'GCC Cuts at: 0 151 231 1674 Size: 151 80 1443 Fragments arranged by size: 1443 151 80 SmaI CCC'GGG Cuts at: 0 37 100 274 1674 Size: 37 63 174 1400 Fragments arranged by size: 1400 174 63 37 StyI C'CwwG_G Cuts at: 0 617 783 795 831 1085 1674 Size: 617 166 12 36 254 589 Fragments arranged by size: 617 589 254 166 36 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1194 1674 Size: 1194 480 TatI w'GTAC_w Cuts at: 0 1646 1674 Size: 1646 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1479 1511 1674 Size: 1479 32 163 Fragments arranged by size: 1479 163 32 XmaI C'CCGG_G Cuts at: 0 35 98 272 1674 Size: 35 63 174 1402 Fragments arranged by size: 1402 174 63 35 XmnI GAAnn'nnTTC Cuts at: 0 1636 1674 Size: 1636 38 Enzymes that do cut: AcuI AfeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BanI BanII BbeI BbsI BciVI BglI BglII Bme1580I BmtI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EagI EciI Eco57MI EcoO109I EcoRV FspI FspAI HaeII Hin4I HincII HindIII KasI MmeI MslI MspA1I NarI NcoI NheI PasI PflMI PfoI PpuMI PspOMI PvuI PvuII SfcI SfoI SmaI StyI TaqII TatI TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AloI AscI AsiSI AvrII BaeI BamHI BbvCI BcgI BclI BfrBI BlpI BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BspMI BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI FalI FseI HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI ZraI