Restriction map for pFN21AE6379
 (Circular) (Six-base) MAPSORT of: pFN21AE6379.seq  Check: 4049  from: 1  to: 5686



pFN21AE6379  5686 bp

 With 182 enzymes: SgfI * 

                        March 18, 2015 14:51  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3496     278 
   Size:         53      83     186     936    1960    2468

  Fragments arranged by size: 

                2468    1960     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2524    3029    1937 
   Size:        587     505    4594

  Fragments arranged by size: 

                4594     587     505
 
Acc65I G'GTAC_C

Cuts at:   3007    3007 
   Size:       5686
 
AclI AA'CG_TT

Cuts at:   1563    3813    4186    1563 
   Size:       2250     373    3063

  Fragments arranged by size: 

                3063    2250     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2118    3749    5133    5401    1632 
   Size:        486    1631    1384     268    1917

  Fragments arranged by size: 

                1917    1631    1384     486     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5489
 
AflIII A'CryG_T

Cuts at:   1203    4585    5562    1203 
   Size:       3382     977    1327

  Fragments arranged by size: 

                3382    1327     977
 
AgeI A'CCGG_T

Cuts at:   4517    4517 
   Size:       5686
 
AhdI GACnn_n'nnGTC

Cuts at:   4415    4415 
   Size:       5686
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5686
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5686
 
AlwNI CAG_nnn'CTG

Cuts at:   3429    5001    3429 
   Size:       1572    4114
 
ApaLI G'TGCA_C

Cuts at:   2718    3743    4899    2718 
   Size:       1025    1156    3505

  Fragments arranged by size: 

                3505    1156    1025
 
ApoI r'AATT_y

Cuts at:   1493    2216    2264    2333    2534    2586    2804    2827    2995 
   Size:        723      48      69     201      52     218      23     168

Cuts at:   2995    3221    3373    5482    1493 
   Size:        226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     723     226     218     201     168     152
                  69      52      48      23
 
AseI AT'TA_AT

Cuts at:    160    1032    4240     160 
   Size:        872    3208    1606

  Fragments arranged by size: 

                3208    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5686
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3011    1870 
   Size:         73    1068    4545

  Fragments arranged by size: 

                4545    1068      73
 
BamHI G'GATC_C

Cuts at:   3016    3383    3016 
   Size:        367    5319
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2372    3007    4462     618 
   Size:        325     217     627     585     635    1455    1842

  Fragments arranged by size: 

                1842    1455     635     627     585     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3005    1391 
   Size:       1614    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2425    2856     928 
   Size:        971     526     431    3758

  Fragments arranged by size: 

                3758     971     526     431
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3877    3911    1903 
   Size:         34    1940      34    3678

  Fragments arranged by size: 

                3678    1940      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3578    4788    3578 
   Size:       1210    4476
 
BclI T'GATC_A

Cuts at:   1548    2637    1548 
   Size:       1089    4597
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4297     136 
   Size:        107     122      71     631     605    2625    1525

  Fragments arranged by size: 

                2625    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5681    5681 
   Size:       5686
 
BlpI GC'TnA_GC

Cuts at:   3103    3103 
   Size:       5686
 
Bme1580I G_kGCm'C

Cuts at:   1790    2722    3747    4903    1790 
   Size:        932    1025    1156    2573

  Fragments arranged by size: 

                2573    1156    1025     932
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5686
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2661    3417    4375 
   Size:        416      53      83     399      24    1244     756     958

Cuts at:   4375     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1244     958     756     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5686
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2274    2913    2274 
   Size:        639    5047
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2319    2934    4346    1239 
   Size:       1080     615    1412    2579

  Fragments arranged by size: 

                2579    1412    1080     615
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2407    3811    4676    4974    2407 
   Size:       1404     865     298    3119

  Fragments arranged by size: 

                3119    1404     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4349     882 
   Size:        617    2850    2219

  Fragments arranged by size: 

                2850    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3477     493 
   Size:        711    2273    2702

  Fragments arranged by size: 

                2702    2273     711
 
BsaBI GATnn'nnATC

Cuts at:   2944    5680    2944 
   Size:       2736    2950
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3493    3875    4510     275 
   Size:         53      83     186     936    1960     382     635    1451

  Fragments arranged by size: 

                1960    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2853    4119    4517    4791    4938    5349    2853 
   Size:       1266     398     274     147     411    3190

  Fragments arranged by size: 

                3190    1266     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2130    2160    1178 
   Size:         30     165      30     727      30    4704

  Fragments arranged by size: 

                4704     727     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5686
 
BseYI C'CCAG_C

Cuts at:   4889    5385    4889 
   Size:        496    5190
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5113
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3136    3897    4925    1540 
   Size:        275     186    1135     761    1028    2301

  Fragments arranged by size: 

                2301    1135    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2722    3747    3832    4903    2722 
   Size:       1025      85    1071    3505

  Fragments arranged by size: 

                3505    1071    1025      85
 
BsmI GAATG_Cn'

Cuts at:   3197    3290    3197 
   Size:         93    5593
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2722    3005    3747    3832    4903    1391 
   Size:        399     932     283     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     932     742     399     283      85
 
BspEI T'CCGG_A

Cuts at:   5349    5349 
   Size:       5686
 
BspHI T'CATG_A

Cuts at:   2260    3575    5305    2260 
   Size:       1315    1730    2641

  Fragments arranged by size: 

                2641    1730    1315
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3041     844 
   Size:       2197    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3573    1394 
   Size:        429    1750    3507

  Fragments arranged by size: 

                3507    1750     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4181    4355      65 
   Size:       1183    2933     174    1396

  Fragments arranged by size: 

                2933    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4330    4517    1700 
   Size:        110    2520     187    2869

  Fragments arranged by size: 

                2869    2520     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5686
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5686
 
BssSI C'ACGA_G

Cuts at:   3746    4758    3746 
   Size:       1012    4674
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5686
 
BstBI TT'CG_AA

Cuts at:   4490    4490 
   Size:       5686
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5686
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2080    2080 
   Size:       5686
 
BstYI r'GATC_y

Cuts at:   1972    3016    3383    3768    3785    5237    5323    5335    5681 
   Size:       1044     367     385      17    1452      86      12     346

Cuts at:   5681    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1044     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5134
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4947

  Fragments arranged by size: 

                4947     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3202    3996    4016    3202 
   Size:        794      20    4872

  Fragments arranged by size: 

                4872     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2046    1554 
   Size:        492    5194
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5651
 
DraI TTT'AAA

Cuts at:   2565    2990    2565 
   Size:        425    5261
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4693     809 
   Size:        507    3377    1802

  Fragments arranged by size: 

                3377    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3133    4022    5651 
   Size:         54    1425      50     275      18    1303     889    1629

Cuts at:   5651       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1303     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3133    1537 
   Size:        275    1321    4090

  Fragments arranged by size: 

                4090    1321     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2132    3616    1353 
   Size:        779    1484    3423

  Fragments arranged by size: 

                3423    1484     779
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4273    4647    4793     387 
   Size:       1064     138    2684     374     146    1280

  Fragments arranged by size: 

                2684    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2118    2319    2934    3749    4346    5133    5401 
   Size:        393     486     201     615     815     597     787     268

Cuts at:   5401    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     815     787     615     597     486     393     268
                 201
 
EcoRI G'AATT_C

Cuts at:   2995    3373    2995 
   Size:        378    5308
 
FspI TGC'GCA

Cuts at:   4192    4192 
   Size:       5686
 
HaeII r_GCGC'y

Cuts at:   1678    4833    1678 
   Size:       3155    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2126    2158    4327    4359    4401 
   Size:         32     135      32     554      32    2169      32      42

Cuts at:   4401    4433    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2169     554     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2485    3030    3276     669 
   Size:        988     281     547     545     246    3079

  Fragments arranged by size: 

                3079     988     547     545     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5686
 
HpaI GTT'AAC

Cuts at:   2485    3276    2485 
   Size:        791    4895
 
KpnI G_GTAC'C

Cuts at:   3011    3011 
   Size:       5686
 
MfeI C'AATT_G

Cuts at:   3285    3285 
   Size:       5686
 
MluI A'CGCG_T

Cuts at:   5562    5562 
   Size:       5686
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2560    2804    4800    4984    1305 
   Size:       1255     244    1996     184    2007

  Fragments arranged by size: 

                2007    1996    1255     244     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3864

  Fragments arranged by size: 

                3864    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3644    4003    4162     518 
   Size:        648    2478     359     159    2042

  Fragments arranged by size: 

                2478    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2635    3102    3779    4927    5172    1606 
   Size:       1029     467     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1029     677     467     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5134
 
NdeI CA'TA_TG

Cuts at:    387    2356    2622     387 
   Size:       1969     266    3451

  Fragments arranged by size: 

                3451    1969     266
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5686
 
NotI GC'GGCC_GC

Cuts at:   3133    3133 
   Size:       5686
 
NspI r_CATG'y

Cuts at:   1132    1229    3044    4589    1132 
   Size:         97    1815    1545    2229

  Fragments arranged by size: 

                2229    1815    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5686
 
PciI A'CATG_T

Cuts at:   4585    4585 
   Size:       5686
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5686
 
PmeI GTTT'AAAC

Cuts at:   2990    2990 
   Size:       5686
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3755    3787    5268    5300    3755 
   Size:         32    1481      32    4141

  Fragments arranged by size: 

                4141    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5686
 
PsiI TTA'TAA

Cuts at:   3256    3256 
   Size:       5686
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2277    2309    2277 
   Size:         32    5654
 
PstI C_TGCA'G

Cuts at:    830    3038     830 
   Size:       2208    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5686
 
PvuII CAG'CTG

Cuts at:   2635    2635 
   Size:       5686
 
SalI G'TCGA_C

Cuts at:   1936    3028    1936 
   Size:       1092    4594
 
SbfI CC_TGCA'GG

Cuts at:   3038    3038 
   Size:       5686
 
ScaI AGT'ACT

Cuts at:   3934    5646    3934 
   Size:       1712    3974
 
SfcI C'TryA_G

Cuts at:    826    1045    3034    4169    4850    5041     826 
   Size:        219    1989    1135     681     191    1471

  Fragments arranged by size: 

                1989    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5686
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5686
 
SmaI CCC'GGG

Cuts at:   1872    3013    1872 
   Size:       1141    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2386    3790    4691    4953     820 
   Size:        197     926     443    1404     901     262    1553

  Fragments arranged by size: 

                1553    1404     926     901     443     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3477     493 
   Size:       2984    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5686
 
SphI G_CATG'C

Cuts at:   1132    3044    1132 
   Size:       1912    3774
 
SspI AAT'ATT

Cuts at:      5      52    3610       5 
   Size:         47    3558    2081

  Fragments arranged by size: 

                3558    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5686
 
StyI C'CwwG_G

Cuts at:    513    1065    2200    3125     513 
   Size:        552    1135     925    3074

  Fragments arranged by size: 

                3074    1135     925     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3713    3730    3883    4068    4513     325 
   Size:       1255    2133      17     153     185     445    1498

  Fragments arranged by size: 

                2133    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3932    5644      96 
   Size:        274      80      33      51    1443    1955    1712     138

  Fragments arranged by size: 

                1955    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5646    5678    5646 
   Size:         32    5654
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5686
 
XbaI T'CTAG_A

Cuts at:   3022    3022 
   Size:       5686
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5686
 
XmaI C'CCGG_G

Cuts at:   1870    3011    1870 
   Size:       1141    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2739    3815    2739 
   Size:       1076    4610
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3494     276 
   Size:         53      83     186     936    1960    2468

  Fragments arranged by size: 

                2468    1960     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI     PsrI     PstI     PvuI    PvuII     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI
    PpuMI   PspOMI    PspXI    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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