################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AviII AvrII BbeI BbrPI BbvCI BcgI BlnI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspEI BsrFI BssHII Bst1107I BstUI BstZ17I CfoI Cfr10I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### ApaI 2518 GGGCCC GGGCC^C AseI 1378 ATTAAT AT^TAAT AsnI 1378 ATTAAT AT^TAAT Asp700I 727 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 2383 GAGCTC G[AT]GC[AT]^C AspI 2404 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 620 CCCGAG C^[CT]CG[GA]G BalI 2111 TGGCCA TGG^CCA BamHI 773 GGATCC G^GATCC BanI 2525 GGCACC G^G[CT][GA]CC BciVI 1170 GGATAC GTATCC(6/5) BclI 2129 TGATCA T^GATCA BglI 379 GCCTTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1835 AGATCT A^GATCT BsaBI 1804 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 2383 GAGCTC G[AT]GC[AT]^C BsoBI 620 CCCGAG C^[CT]CG[GA]G Bsp1407I 1442 TGTACA T^GTACA BspDI 31 ATCGAT AT^CGAT BspMI 48 GCAGGT ACCTGC(4/8) BsrBI 976 GAGCGG CCGCTC(-3/-3) BsrGI 1442 TGTACA T^GTACA BssSI 1927 CACGAG CACGAG(-5/-1) BstBI 153 TTCGAA TT^CGAA BstEII 99 GGTTACC G^GT[ACGT]ACC BstPI 99 GGTTACC G^GT[ACGT]ACC ClaI 31 ATCGAT AT^CGAT DraI 246 TTTAAA TTT^AAA DraII 2518 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoNI 1914 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 2518 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 99 GGTTACC G^GT[ACGT]ACC FauI 1609 GCGGG CCCGC(4/6) FbaI 2129 TGATCA T^GATCA HindIII 526 AAGCTT A^AGCTT HpaI 1711 GTTAAC GTT^AAC MamI 1804 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2111 TGGCCA TGG^CCA MscI 2111 TGGCCA TGG^CCA NcoI 1433 CCATGG C^CATGG NspV 153 TTCGAA TT^CGAA PflFI 2404 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1378 ATTAAT AT^TAAT SacI 2383 GAGCTC GAGCT^C SapI 1012 GCTCTTC GCTCTTC(1/4) ScaI 1726 AGTACT AGT^ACT SfuI 153 TTCGAA TT^CGAA SspBI 1442 TGTACA T^GTACA SspI 1078 AATATT AAT^ATT Tth111I 2404 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 727 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflII 1572 CTTAAG C^TTAAG 1776 CTTAAG BbsI 1104 GAAGAC GAAGAC(2/6) 2672 GAAGAC BfrI 1572 CTTAAG C^TTAAG 1776 CTTAAG BlpI 2020 GCTGAGC GC^T[ACGT]AGC 2621 GCTCAGC Bpu1102I 2020 GCTGAGC GC^T[ACGT]AGC 2621 GCTCAGC BpuAI 1104 GAAGAC GAAGAC(2/6) 2672 GAAGAC BsaI 1112 GAGACC GGTCTC(1/5) 1577 GAGACC BseRI 877 GAGGAG GAGGAG(10/8) 2378 GAGGAG BsgI 1385 CTGCAC GTGCAG(16/14) 2603 GTGCAG BsrDI 2478 CATTGC GCAATG(2/0) 2645 CATTGC BstAPI 1285 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1633 GCAAGAGTTGC BstXI 522 CCAGAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1346 CCAATCATTTGG CelII 2020 GCTGAGC GC^T[ACGT]AGC 2621 GCTCAGC DrdI 468 GACATTTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1406 GACCCAAAAGTC DsaI 65 CCGTGG C^C[GA][CT]GG 1433 CCATGG EaeI 1215 TGGCCG [CT]^GGCC[GA] 2111 TGGCCA EarI 1013 CTCTTC CTCTTC(1/4) 1918 GAAGAG EcoRV 1855 GATATC GAT^ATC 2211 GATATC EcoT14I 1433 CCATGG C^C[AT][AT]GG 1766 CCTTGG Ksp632I 1013 CTCTTC CTCTTC(1/4) 1918 GAAGAG PflMI 1536 CCATATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2367 CCAGAACCTGG PstI 46 CTGCAG CTGCA^G 2343 CTGCAG SfaNI 1585 GATGC GCATC(5/9) 2062 GATGC StyI 1433 CCATGG C^C[AT][AT]GG 1766 CCTTGG TaqI 32 TCGA T^CGA 154 TCGA TthHB8I 32 TCGA T^CGA 154 TCGA Van91I 1536 CCATATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2367 CCAGAACCTGG XcmI 2505 CCAGAGTCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2530 CCAGTTGTGATGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspLU11I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII Eco81I EcoRII Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfcI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII