Restriction map for pFN21AB8536
 (Linear) (Six-base) MAPSORT of: KIBB8536.seq  Check: 9280  from: 1  to: 2685



KIBB8536.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652    1000    1502    2685 
   Size:        652     348     502    1183

  Fragments arranged by size: 

                1183     652     502     348
 
AflII C'TTAA_G

Cuts at:      0    1572    1776    2685 
   Size:       1572     204     909

  Fragments arranged by size: 

                1572     909     204
 
AflIII A'CryG_T

Cuts at:      0     447    1094    1470    2120    2599    2685 
   Size:        447     647     376     650     479      86

  Fragments arranged by size: 

                 650     647     479     447     376      86
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1916    1948    2685 
   Size:       1916      32     737

  Fragments arranged by size: 

                1916     737      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      45     874    1023    2373    2685 
   Size:         45     829     149    1350     312

  Fragments arranged by size: 

                1350     829     312     149      45
 
ApaI G_GGCC'C

Cuts at:      0    2522    2685 
   Size:       2522     163
 
ApoI r'AATT_y

Cuts at:      0     201     546     731     843    1741    2436    2662    2685 
   Size:        201     345     185     112     898     695     226      23

  Fragments arranged by size: 

                 898     695     345     226     201     185     112      23
            
 
AseI AT'TA_AT

Cuts at:      0    1379    2685 
   Size:       1379    1306
 
AvaI C'yCGr_G

Cuts at:      0     620    2685 
   Size:        620    2065
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1163    1196    1545    1578    2685 
   Size:       1163      33     349      33    1107

  Fragments arranged by size: 

                1163    1107     349      33      33
 
BamHI G'GATC_C

Cuts at:      0     773    2685 
   Size:        773    1912
 
BanI G'GyrC_C

Cuts at:      0    2525    2685 
   Size:       2525     160
 
BanII G_rGCy'C

Cuts at:      0     621    2387    2522    2685 
   Size:        621    1766     135     163

  Fragments arranged by size: 

                1766     621     163     135
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1111    2679    2685 
   Size:       1111    1568       6

  Fragments arranged by size: 

                1568    1111       6
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1164    2685 
   Size:       1164    1521
 
BclI T'GATC_A

Cuts at:      0    2129    2685 
   Size:       2129     556
 
BglI GCCn_nnn'nGGC

Cuts at:      0     385    2685 
   Size:        385    2300
 
BglII A'GATC_T

Cuts at:      0    1835    2685 
   Size:       1835     850
 
BlpI GC'TnA_GC

Cuts at:      0    2021    2622    2685 
   Size:       2021     601      63

  Fragments arranged by size: 

                2021     601      63
 
Bme1580I G_kGCm'C

Cuts at:      0     828    2269    2522    2685 
   Size:        828    1441     253     163

  Fragments arranged by size: 

                1441     828     253     163
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2524    2685 
   Size:       2524     161
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2401    2685 
   Size:       2401     284
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     493     859    1918    2685 
   Size:        100     393     366    1059     767

  Fragments arranged by size: 

                1059     767     393     366     100
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292    2685 
   Size:        292    2393
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1106    1571    2685 
   Size:       1106     465    1114

  Fragments arranged by size: 

                1114    1106     465
 
BsaBI GATnn'nnATC

Cuts at:      0    1808    2685 
   Size:       1808     877
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     892    2393    2685 
   Size:        892    1501     292

  Fragments arranged by size: 

                1501     892     292
 
BseYI C'CCAG_C

Cuts at:      0    2515    2685 
   Size:       2515     170
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1370    2624    2685 
   Size:       1370    1254      61

  Fragments arranged by size: 

                1370    1254      61
 
BsiHKAI G_wGCw'C

Cuts at:      0    2387    2685 
   Size:       2387     298
 
BsmI GAATG_Cn'

Cuts at:      0     654     681     744     997    1949    2451    2685 
   Size:        654      27      63     253     952     502     234

  Fragments arranged by size: 

                 952     654     502     253     234      63      27
 
Bsp1286I G_dGCh'C

Cuts at:      0     621     828    2269    2387    2522    2685 
   Size:        621     207    1441     118     135     163

  Fragments arranged by size: 

                1441     621     207     163     135     118
 
BspHI T'CATG_A

Cuts at:      0    1254    1851    1871    2132    2654    2685 
   Size:       1254     597      20     261     522      31

  Fragments arranged by size: 

                1254     597     522     261      31      20
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      39    2685 
   Size:         39    2646
 
BsrBI CCG'CTC

Cuts at:      0     978    2685 
   Size:        978    1707
 
BsrDI GCAATG_nn'

Cuts at:      0    2477    2644    2685 
   Size:       2477     167      41

  Fragments arranged by size: 

                2477     167      41
 
BsrGI T'GTAC_A

Cuts at:      0    1442    2685 
   Size:       1442    1243
 
BssSI C'ACGA_G

Cuts at:      0    1927    2685 
   Size:       1927     758
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1291    1639    2685 
   Size:       1291     348    1046

  Fragments arranged by size: 

                1291    1046     348
 
BstBI TT'CG_AA

Cuts at:      0     154    2685 
   Size:        154    2531
 
BstEII G'GTnAC_C

Cuts at:      0      99    2685 
   Size:         99    2586
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     529    1353    2685 
   Size:        529     824    1332

  Fragments arranged by size: 

                1332     824     529
 
BstYI r'GATC_y

Cuts at:      0      42     773    1020    1835    2271    2363    2685 
   Size:         42     731     247     815     436      92     322

  Fragments arranged by size: 

                 815     731     436     322     247      92      42
 
Bsu36I CC'TnA_GG

Cuts at:      0      38     803    1997    2685 
   Size:         38     765    1194     688

  Fragments arranged by size: 

                1194     765     688      38
 
BtgI C'CryG_G

Cuts at:      0      65    1433    2685 
   Size:         65    1368    1252

  Fragments arranged by size: 

                1368    1252      65
 
ClaI AT'CG_AT

Cuts at:      0      32    2685 
   Size:         32    2653
 
DraI TTT'AAA

Cuts at:      0     248    2685 
   Size:        248    2437
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     474    1412    2685 
   Size:        474     938    1273

  Fragments arranged by size: 

                1273     938     474
 
EaeI y'GGCC_r

Cuts at:      0    1215    2111    2685 
   Size:       1215     896     574

  Fragments arranged by size: 

                1215     896     574
 
EarI CTCTTCn'nnn_

Cuts at:      0    1019    1913    2685 
   Size:       1019     894     772

  Fragments arranged by size: 

                1019     894     772
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     958    2685 
   Size:        958    1727
 
EcoICRI GAG'CTC

Cuts at:      0    2385    2685 
   Size:       2385     300
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     493     652     859    1000    1502    1918    2685 
   Size:        100     393     159     207     141     502     416     767

  Fragments arranged by size: 

                 767     502     416     393     207     159     141     100
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1918    2685 
   Size:       1918     767
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2519    2685 
   Size:       2519     166
 
EcoRV GAT'ATC

Cuts at:      0    1857    2213    2685 
   Size:       1857     356     472

  Fragments arranged by size: 

                1857     472     356
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     152     184     392     424     571     603    1138    1167 
   Size:        152      32     208      32     147      32     535      29

Cuts at:   1167    1170    1199    1853    1885    2244    2276    2372    2404 
   Size:          3      29     654      32     359      32      96      32

Cuts at:   2404    2685 
   Size:        281

  Fragments arranged by size: 

                 654     535     359     281     208     152     147      96
                  32      32      32      32      32      32      29      29
                   3
 
HincII GTy'rAC

Cuts at:      0    1307    1554    1713    2685 
   Size:       1307     247     159     972

  Fragments arranged by size: 

                1307     972     247     159
 
HindIII A'AGCT_T

Cuts at:      0     526    2685 
   Size:        526    2159
 
HpaI GTT'AAC

Cuts at:      0    1713    2685 
   Size:       1713     972
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1781    2685 
   Size:       1781     904
 
MscI TGG'CCA

Cuts at:      0    2113    2685 
   Size:       2113     572
 
MslI CAynn'nnrTG

Cuts at:      0     686    1438    1469    2125    2685 
   Size:        686     752      31     656     560

  Fragments arranged by size: 

                 752     686     656     560      31
 
MspA1I CmG'CkG

Cuts at:      0       7     313    2608    2685 
   Size:          7     306    2295      77

  Fragments arranged by size: 

                2295     306      77       7
 
NcoI C'CATG_G

Cuts at:      0    1433    2685 
   Size:       1433    1252
 
NspI r_CATG'y

Cuts at:      0     451    1098    1474    2124    2685 
   Size:        451     647     376     650     561

  Fragments arranged by size: 

                 650     647     561     451     376
 
PasI CC'CwG_GG

Cuts at:      0     816    2685 
   Size:        816    1869
 
PciI A'CATG_T

Cuts at:      0     447    1094    1470    2120    2685 
   Size:        447     647     376     650     565

  Fragments arranged by size: 

                 650     647     565     447     376
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1542    2373    2685 
   Size:       1542     831     312

  Fragments arranged by size: 

                1542     831     312
 
PfoI T'CCnGG_A

Cuts at:      0    1166    2685 
   Size:       1166    1519
 
PspOMI G'GGCC_C

Cuts at:      0    2518    2685 
   Size:       2518     167
 
PstI C_TGCA'G

Cuts at:      0      50    2347    2685 
   Size:         50    2297     338

  Fragments arranged by size: 

                2297     338      50
 
PvuII CAG'CTG

Cuts at:      0       7     313    2608    2685 
   Size:          7     306    2295      77

  Fragments arranged by size: 

                2295     306      77       7
 
SacI G_AGCT'C

Cuts at:      0    2387    2685 
   Size:       2387     298
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1019    2685 
   Size:       1019    1666
 
ScaI AGT'ACT

Cuts at:      0    1728    2685 
   Size:       1728     957
 
SfcI C'TryA_G

Cuts at:      0      46     239    2343    2450    2685 
   Size:         46     193    2104     107     235

  Fragments arranged by size: 

                2104     235     193     107      46
 
SmlI C'TyrA_G

Cuts at:      0     271    1572    1776    2685 
   Size:        271    1301     204     909

  Fragments arranged by size: 

                1301     909     271     204
 
SspI AAT'ATT

Cuts at:      0    1080    2685 
   Size:       1080    1605
 
StyI C'CwwG_G

Cuts at:      0    1433    1766    2685 
   Size:       1433     333     919

  Fragments arranged by size: 

                1433     919     333
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1972    2543    2685 
   Size:       1972     571     142

  Fragments arranged by size: 

                1972     571     142
 
TatI w'GTAC_w

Cuts at:      0    1034    1442    1726    2685 
   Size:       1034     408     284     959

  Fragments arranged by size: 

                1034     959     408     284
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     758     790    1348    1380    2685 
   Size:        758      32     558      32    1305

  Fragments arranged by size: 

                1305     758     558      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2407    2685 
   Size:       2407     278
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2512    2537    2685 
   Size:       2512      25     148

  Fragments arranged by size: 

                2512     148      25
 
XmnI GAAnn'nnTTC

Cuts at:      0     731    2685 
   Size:        731    1954
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AloI    AlwNI     ApaI     ApoI     AseI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaBI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     ClaI     DraI     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
    Hin4I   HincII  HindIII     HpaI     MmeI     MscI     MslI   MspA1I
     NcoI     NspI     PasI     PciI    PflMI     PfoI   PspOMI     PstI
    PvuII     SacI     SapI     ScaI     SfcI     SmlI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI    ApaLI     AscI    AsiSI    AvrII     BbeI    BbvCI
     BcgI    BfrBI    BmgBI     BmtI   Bpu10I    BsaAI    BsaHI    BsaWI
    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BsrFI   BssHII  BstZ17I
    BtgZI     BtsI    CspCI   DraIII     EagI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     XbaI     XhoI     XmaI     ZraI
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