(Linear) (Six-base) MAPSORT of: KIBB8536.seq Check: 9280 from: 1 to: 2685 KIBB8536.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 652 1000 1502 2685 Size: 652 348 502 1183 Fragments arranged by size: 1183 652 502 348 AflII C'TTAA_G Cuts at: 0 1572 1776 2685 Size: 1572 204 909 Fragments arranged by size: 1572 909 204 AflIII A'CryG_T Cuts at: 0 447 1094 1470 2120 2599 2685 Size: 447 647 376 650 479 86 Fragments arranged by size: 650 647 479 447 376 86 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1916 1948 2685 Size: 1916 32 737 Fragments arranged by size: 1916 737 32 AlwNI CAG_nnn'CTG Cuts at: 0 45 874 1023 2373 2685 Size: 45 829 149 1350 312 Fragments arranged by size: 1350 829 312 149 45 ApaI G_GGCC'C Cuts at: 0 2522 2685 Size: 2522 163 ApoI r'AATT_y Cuts at: 0 201 546 731 843 1741 2436 2662 2685 Size: 201 345 185 112 898 695 226 23 Fragments arranged by size: 898 695 345 226 201 185 112 23 AseI AT'TA_AT Cuts at: 0 1379 2685 Size: 1379 1306 AvaI C'yCGr_G Cuts at: 0 620 2685 Size: 620 2065 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1163 1196 1545 1578 2685 Size: 1163 33 349 33 1107 Fragments arranged by size: 1163 1107 349 33 33 BamHI G'GATC_C Cuts at: 0 773 2685 Size: 773 1912 BanI G'GyrC_C Cuts at: 0 2525 2685 Size: 2525 160 BanII G_rGCy'C Cuts at: 0 621 2387 2522 2685 Size: 621 1766 135 163 Fragments arranged by size: 1766 621 163 135 BbsI GAAGACnn'nnnn_ Cuts at: 0 1111 2679 2685 Size: 1111 1568 6 Fragments arranged by size: 1568 1111 6 BciVI GTATCCnnnnn_n' Cuts at: 0 1164 2685 Size: 1164 1521 BclI T'GATC_A Cuts at: 0 2129 2685 Size: 2129 556 BglI GCCn_nnn'nGGC Cuts at: 0 385 2685 Size: 385 2300 BglII A'GATC_T Cuts at: 0 1835 2685 Size: 1835 850 BlpI GC'TnA_GC Cuts at: 0 2021 2622 2685 Size: 2021 601 63 Fragments arranged by size: 2021 601 63 Bme1580I G_kGCm'C Cuts at: 0 828 2269 2522 2685 Size: 828 1441 253 163 Fragments arranged by size: 1441 828 253 163 BmrI ACTGGGnnnn_n' Cuts at: 0 2524 2685 Size: 2524 161 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2401 2685 Size: 2401 284 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 493 859 1918 2685 Size: 100 393 366 1059 767 Fragments arranged by size: 1059 767 393 366 100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 2685 Size: 292 2393 BsaI GGTCTCn'nnnn_ Cuts at: 0 1106 1571 2685 Size: 1106 465 1114 Fragments arranged by size: 1114 1106 465 BsaBI GATnn'nnATC Cuts at: 0 1808 2685 Size: 1808 877 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 892 2393 2685 Size: 892 1501 292 Fragments arranged by size: 1501 892 292 BseYI C'CCAG_C Cuts at: 0 2515 2685 Size: 2515 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1370 2624 2685 Size: 1370 1254 61 Fragments arranged by size: 1370 1254 61 BsiHKAI G_wGCw'C Cuts at: 0 2387 2685 Size: 2387 298 BsmI GAATG_Cn' Cuts at: 0 654 681 744 997 1949 2451 2685 Size: 654 27 63 253 952 502 234 Fragments arranged by size: 952 654 502 253 234 63 27 Bsp1286I G_dGCh'C Cuts at: 0 621 828 2269 2387 2522 2685 Size: 621 207 1441 118 135 163 Fragments arranged by size: 1441 621 207 163 135 118 BspHI T'CATG_A Cuts at: 0 1254 1851 1871 2132 2654 2685 Size: 1254 597 20 261 522 31 Fragments arranged by size: 1254 597 522 261 31 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 39 2685 Size: 39 2646 BsrBI CCG'CTC Cuts at: 0 978 2685 Size: 978 1707 BsrDI GCAATG_nn' Cuts at: 0 2477 2644 2685 Size: 2477 167 41 Fragments arranged by size: 2477 167 41 BsrGI T'GTAC_A Cuts at: 0 1442 2685 Size: 1442 1243 BssSI C'ACGA_G Cuts at: 0 1927 2685 Size: 1927 758 BstAPI GCAn_nnn'nTGC Cuts at: 0 1291 1639 2685 Size: 1291 348 1046 Fragments arranged by size: 1291 1046 348 BstBI TT'CG_AA Cuts at: 0 154 2685 Size: 154 2531 BstEII G'GTnAC_C Cuts at: 0 99 2685 Size: 99 2586 BstXI CCAn_nnnn'nTGG Cuts at: 0 529 1353 2685 Size: 529 824 1332 Fragments arranged by size: 1332 824 529 BstYI r'GATC_y Cuts at: 0 42 773 1020 1835 2271 2363 2685 Size: 42 731 247 815 436 92 322 Fragments arranged by size: 815 731 436 322 247 92 42 Bsu36I CC'TnA_GG Cuts at: 0 38 803 1997 2685 Size: 38 765 1194 688 Fragments arranged by size: 1194 765 688 38 BtgI C'CryG_G Cuts at: 0 65 1433 2685 Size: 65 1368 1252 Fragments arranged by size: 1368 1252 65 ClaI AT'CG_AT Cuts at: 0 32 2685 Size: 32 2653 DraI TTT'AAA Cuts at: 0 248 2685 Size: 248 2437 DrdI GACnn_nn'nnGTC Cuts at: 0 474 1412 2685 Size: 474 938 1273 Fragments arranged by size: 1273 938 474 EaeI y'GGCC_r Cuts at: 0 1215 2111 2685 Size: 1215 896 574 Fragments arranged by size: 1215 896 574 EarI CTCTTCn'nnn_ Cuts at: 0 1019 1913 2685 Size: 1019 894 772 Fragments arranged by size: 1019 894 772 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 958 2685 Size: 958 1727 EcoICRI GAG'CTC Cuts at: 0 2385 2685 Size: 2385 300 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 493 652 859 1000 1502 1918 2685 Size: 100 393 159 207 141 502 416 767 Fragments arranged by size: 767 502 416 393 207 159 141 100 EcoNI CCTnn'n_nnAGG Cuts at: 0 1918 2685 Size: 1918 767 EcoO109I rG'GnC_Cy Cuts at: 0 2519 2685 Size: 2519 166 EcoRV GAT'ATC Cuts at: 0 1857 2213 2685 Size: 1857 356 472 Fragments arranged by size: 1857 472 356 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 152 184 392 424 571 603 1138 1167 Size: 152 32 208 32 147 32 535 29 Cuts at: 1167 1170 1199 1853 1885 2244 2276 2372 2404 Size: 3 29 654 32 359 32 96 32 Cuts at: 2404 2685 Size: 281 Fragments arranged by size: 654 535 359 281 208 152 147 96 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 0 1307 1554 1713 2685 Size: 1307 247 159 972 Fragments arranged by size: 1307 972 247 159 HindIII A'AGCT_T Cuts at: 0 526 2685 Size: 526 2159 HpaI GTT'AAC Cuts at: 0 1713 2685 Size: 1713 972 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1781 2685 Size: 1781 904 MscI TGG'CCA Cuts at: 0 2113 2685 Size: 2113 572 MslI CAynn'nnrTG Cuts at: 0 686 1438 1469 2125 2685 Size: 686 752 31 656 560 Fragments arranged by size: 752 686 656 560 31 MspA1I CmG'CkG Cuts at: 0 7 313 2608 2685 Size: 7 306 2295 77 Fragments arranged by size: 2295 306 77 7 NcoI C'CATG_G Cuts at: 0 1433 2685 Size: 1433 1252 NspI r_CATG'y Cuts at: 0 451 1098 1474 2124 2685 Size: 451 647 376 650 561 Fragments arranged by size: 650 647 561 451 376 PasI CC'CwG_GG Cuts at: 0 816 2685 Size: 816 1869 PciI A'CATG_T Cuts at: 0 447 1094 1470 2120 2685 Size: 447 647 376 650 565 Fragments arranged by size: 650 647 565 447 376 PflMI CCAn_nnn'nTGG Cuts at: 0 1542 2373 2685 Size: 1542 831 312 Fragments arranged by size: 1542 831 312 PfoI T'CCnGG_A Cuts at: 0 1166 2685 Size: 1166 1519 PspOMI G'GGCC_C Cuts at: 0 2518 2685 Size: 2518 167 PstI C_TGCA'G Cuts at: 0 50 2347 2685 Size: 50 2297 338 Fragments arranged by size: 2297 338 50 PvuII CAG'CTG Cuts at: 0 7 313 2608 2685 Size: 7 306 2295 77 Fragments arranged by size: 2295 306 77 7 SacI G_AGCT'C Cuts at: 0 2387 2685 Size: 2387 298 SapI GCTCTTCn'nnn_ Cuts at: 0 1019 2685 Size: 1019 1666 ScaI AGT'ACT Cuts at: 0 1728 2685 Size: 1728 957 SfcI C'TryA_G Cuts at: 0 46 239 2343 2450 2685 Size: 46 193 2104 107 235 Fragments arranged by size: 2104 235 193 107 46 SmlI C'TyrA_G Cuts at: 0 271 1572 1776 2685 Size: 271 1301 204 909 Fragments arranged by size: 1301 909 271 204 SspI AAT'ATT Cuts at: 0 1080 2685 Size: 1080 1605 StyI C'CwwG_G Cuts at: 0 1433 1766 2685 Size: 1433 333 919 Fragments arranged by size: 1433 919 333 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1972 2543 2685 Size: 1972 571 142 Fragments arranged by size: 1972 571 142 TatI w'GTAC_w Cuts at: 0 1034 1442 1726 2685 Size: 1034 408 284 959 Fragments arranged by size: 1034 959 408 284 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 758 790 1348 1380 2685 Size: 758 32 558 32 1305 Fragments arranged by size: 1305 758 558 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2407 2685 Size: 2407 278 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2512 2537 2685 Size: 2512 25 148 Fragments arranged by size: 2512 148 25 XmnI GAAnn'nnTTC Cuts at: 0 731 2685 Size: 731 1954 Enzymes that do cut: AcuI AflII AflIII AloI AlwNI ApaI ApoI AseI AvaI BaeI BamHI BanI BanII BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaBI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI ClaI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PspOMI PstI PvuII SacI SapI ScaI SfcI SmlI SspI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AgeI AhdI AleI AlfI ApaLI AscI AsiSI AvrII BbeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaAI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrFI BssHII BstZ17I BtgZI BtsI CspCI DraIII EagI EcoRI FalI FseI FspI FspAI HaeII KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI XhoI XmaI ZraI