Restriction map for pFN21AB8468
 (Circular) (Six-base) MAPSORT of: pFN21AB8468.seq  Check: 8717  from: 1  to: 5596



pFN21AB8468  5596 bp

 With 182 enzymes: SgfI * 

                        January 31, 2011 17:47  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3406     278 
   Size:         53      83     186     936    1870    2468

  Fragments arranged by size: 

                2468    1870     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2068    2939    1937 
   Size:        131     871    4594

  Fragments arranged by size: 

                4594     871     131
 
Acc65I G'GTAC_C

Cuts at:   2042    2917    2042 
   Size:        875    4721
 
AclI AA'CG_TT

Cuts at:   1563    3723    4096    1563 
   Size:       2160     373    3063

  Fragments arranged by size: 

                3063    2160     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2186    2285    2629    3659    5043    5311    1632 
   Size:        554      99     344    1030    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1030     554     344     268      99
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5399
 
AflIII A'CryG_T

Cuts at:   1203    4495    5472    1203 
   Size:       3292     977    1327

  Fragments arranged by size: 

                3292    1327     977
 
AgeI A'CCGG_T

Cuts at:   4427    4427 
   Size:       5596
 
AhdI GACnn_n'nnGTC

Cuts at:   4325    4325 
   Size:       5596
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5596
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5596
 
AlwNI CAG_nnn'CTG

Cuts at:   3339    4911    3339 
   Size:       1572    4024
 
ApaLI G'TGCA_C

Cuts at:   3653    4809    3653 
   Size:       1156    4440
 
ApoI r'AATT_y

Cuts at:   1493    2157    2829    2905    3131    3283    5392    1493 
   Size:        664     672      76     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     672     664     226     152      76
 
AseI AT'TA_AT

Cuts at:    160    1032    4150     160 
   Size:        872    3118    1606

  Fragments arranged by size: 

                3118    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5596
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2921    1870 
   Size:         73     978    4545

  Fragments arranged by size: 

                4545     978      73
 
BamHI G'GATC_C

Cuts at:   2926    3293    2926 
   Size:        367    5229
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2042    2576    2917    4372     618 
   Size:        325     217     627     255     534     341    1455    1842

  Fragments arranged by size: 

                1842    1455     627     534     341     325     255     217
            
 
BanII G_rGCy'C

Cuts at:   1391    2915    1391 
   Size:       1524    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2192     928 
   Size:        971     293    4332

  Fragments arranged by size: 

                4332     971     293
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3787    3821    1903 
   Size:         34    1850      34    3678

  Fragments arranged by size: 

                3678    1850      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3488    4698    3488 
   Size:       1210    4386
 
BclI T'GATC_A

Cuts at:   1548    2635    1548 
   Size:       1087    4509
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4207     136 
   Size:        107     122      71     631     605    2535    1525

  Fragments arranged by size: 

                2535    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2513    5591    2513 
   Size:       3078    2518
 
BlpI GC'TnA_GC

Cuts at:   3013    3013 
   Size:       5596
 
Bme1580I G_kGCm'C

Cuts at:   1790    2669    3657    4813    1790 
   Size:        879     988    1156    2573

  Fragments arranged by size: 

                2573    1156     988     879
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5596
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3327    4285     442 
   Size:        416      53      83     399      24    1910     958    1753

  Fragments arranged by size: 

                1910    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5596
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2502    2538    4256    1239 
   Size:       1263      36    1718    2579

  Fragments arranged by size: 

                2579    1718    1263      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3721    4586    4884    3721 
   Size:        865     298    4433

  Fragments arranged by size: 

                4433     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4259     882 
   Size:        617    2760    2219

  Fragments arranged by size: 

                2760    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3387     493 
   Size:        711    2183    2702

  Fragments arranged by size: 

                2702    2183     711
 
BsaBI GATnn'nnATC

Cuts at:   5590    5590 
   Size:       5596
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3403    3785    4420     275 
   Size:         53      83     186     936    1870     382     635    1451

  Fragments arranged by size: 

                1870    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4029    4427    4701    4848    5259    4029 
   Size:        398     274     147     411    4366

  Fragments arranged by size: 

                4366     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5371

  Fragments arranged by size: 

                5371     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5596
 
BseYI C'CCAG_C

Cuts at:   2530    4799    5295    2530 
   Size:       2269     496    2831

  Fragments arranged by size: 

                2831    2269     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5023
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3046    3807    4835    1540 
   Size:        275     186    1045     761    1028    2301

  Fragments arranged by size: 

                2301    1045    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3657    3742    4813    3657 
   Size:         85    1071    4440

  Fragments arranged by size: 

                4440    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3107    3200    3107 
   Size:         93    5503
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2669    2915    3657    3742    4813    1391 
   Size:        399     879     246     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     879     742     399     246      85
 
BspEI T'CCGG_A

Cuts at:   5259    5259 
   Size:       5596
 
BspHI T'CATG_A

Cuts at:   3485    5215    3485 
   Size:       1730    3866
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2951     844 
   Size:       2107    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3483    1394 
   Size:        429    1660    3507

  Fragments arranged by size: 

                3507    1660     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4091    4265      65 
   Size:       1183    2843     174    1396

  Fragments arranged by size: 

                2843    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4240    4427    1700 
   Size:        110    2430     187    2869

  Fragments arranged by size: 

                2869    2430     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5596
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5596
 
BssSI C'ACGA_G

Cuts at:   3656    4668    3656 
   Size:       1012    4584
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5596
 
BstBI TT'CG_AA

Cuts at:   4400    4400 
   Size:       5596
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5596
 
BstYI r'GATC_y

Cuts at:   1972    2260    2513    2926    3293    3678    3695    5147    5233 
   Size:        288     253     413     367     385      17    1452      86

Cuts at:   5233    5245    5591    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     413     385     367     346     288     253
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2256    2617     513 
   Size:        552     939     252     361    3492

  Fragments arranged by size: 

                3492     939     552     361     252
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4857

  Fragments arranged by size: 

                4857     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3112    3906    3926    3112 
   Size:        794      20    4782

  Fragments arranged by size: 

                4782     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5596
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5561
 
DraI TTT'AAA

Cuts at:   2900    2900 
   Size:       5596
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4603     809 
   Size:        507    3287    1802

  Fragments arranged by size: 

                3287    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2687    3043    3932 
   Size:         54    1425      50     275      18     857     356     889

Cuts at:   3932    5561       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     857     356     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3043    1537 
   Size:        275    1231    4090

  Fragments arranged by size: 

                4090    1231     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2118    2365    2648    3526    1353 
   Size:        765     247     283     878    3423

  Fragments arranged by size: 

                3423     878     765     283     247
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4183    4557    4703     387 
   Size:       1064     138    2594     374     146    1280

  Fragments arranged by size: 

                2594    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2186    2285    2502    2538    2629    3659    4256 
   Size:        393     554      99     217      36      91    1030     597

Cuts at:   4256    5043    5311    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524    1030     787     597     554     393     268     217
                  99      91      36
 
EcoRI G'AATT_C

Cuts at:   2905    3283    2905 
   Size:        378    5218
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2290    2290 
   Size:       5596
 
FspI TGC'GCA

Cuts at:   4102    4102 
   Size:       5596
 
HaeII r_GCGC'y

Cuts at:   1678    4743    1678 
   Size:       3065    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2780    2812    4237    4269    4311 
   Size:         32     135      32    1208      32    1425      32      42

Cuts at:   4311    4343    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1425    1208     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2560    2808    2940    3186     669 
   Size:        988     281     622     248     132     246    3079

  Fragments arranged by size: 

                3079     988     622     281     248     246     132
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5596
 
HpaI GTT'AAC

Cuts at:   2808    3186    2808 
   Size:        378    5218
 
KpnI G_GTAC'C

Cuts at:   2046    2921    2046 
   Size:        875    4721
 
MfeI C'AATT_G

Cuts at:   3195    3195 
   Size:       5596
 
MluI A'CGCG_T

Cuts at:   5472    5472 
   Size:       5596
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4710    4894    1305 
   Size:       3405     184    2007

  Fragments arranged by size: 

                3405    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2689      10 
   Size:         54    1425     343     857    2917

  Fragments arranged by size: 

                2917    1425     857     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3554    3913    4072     518 
   Size:        648    2388     359     159    2042

  Fragments arranged by size: 

                2388    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3012    3689    4837    5082    1606 
   Size:       1406     677    1148     245    2120

  Fragments arranged by size: 

                2120    1406    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2256    2617     513 
   Size:        552     939     252     361    3492

  Fragments arranged by size: 

                3492     939     552     361     252
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5596
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5596
 
NotI GC'GGCC_GC

Cuts at:   3043    3043 
   Size:       5596
 
NspI r_CATG'y

Cuts at:   1132    1229    2954    4499    1132 
   Size:         97    1725    1545    2229

  Fragments arranged by size: 

                2229    1725    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5596
 
PciI A'CATG_T

Cuts at:   4495    4495 
   Size:       5596
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5596
 
PmeI GTTT'AAAC

Cuts at:   2900    2900 
   Size:       5596
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3665    3697    5178    5210    3665 
   Size:         32    1481      32    4051

  Fragments arranged by size: 

                4051    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5596
 
PsiI TTA'TAA

Cuts at:   3166    3166 
   Size:       5596
 
PstI C_TGCA'G

Cuts at:    830    2948     830 
   Size:       2118    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5596
 
SalI G'TCGA_C

Cuts at:   1936    2938    1936 
   Size:       1002    4594
 
SbfI CC_TGCA'GG

Cuts at:   2948    2948 
   Size:       5596
 
ScaI AGT'ACT

Cuts at:   2143    3844    5556    2143 
   Size:       1701    1712    2183

  Fragments arranged by size: 

                2183    1712    1701
 
SfcI C'TryA_G

Cuts at:    826    1045    2079    2342    2944    4079    4760    4951     826 
   Size:        219    1034     263     602    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1034     681     602     263     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5596
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5596
 
SmaI CCC'GGG

Cuts at:   1872    2923    1872 
   Size:       1051    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3700    4601    4863     820 
   Size:        197     926    1757     901     262    1553

  Fragments arranged by size: 

                1757    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3387     493 
   Size:       2894    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5596
 
SphI G_CATG'C

Cuts at:   1132    2954    1132 
   Size:       1822    3774
 
SspI AAT'ATT

Cuts at:      5      52    3520       5 
   Size:         47    3468    2081

  Fragments arranged by size: 

                3468    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5596
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2256    2617    3035     513 
   Size:        552     939     252     361     418    3074

  Fragments arranged by size: 

                3074     939     552     418     361     252
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3623    3640    3793    3978    4423     325 
   Size:       1255    2043      17     153     185     445    1498

  Fragments arranged by size: 

                2043    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2141    2298    3842 
   Size:        274      80      33      51    1443     164     157    1544

Cuts at:   3842    5554      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1544    1443     274     164     157     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5556    5588    5556 
   Size:         32    5564
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5596
 
XbaI T'CTAG_A

Cuts at:   2366    2932    2366 
   Size:        566    5030
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2562    2562 
   Size:       5596
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5596
 
XmaI C'CCGG_G

Cuts at:   1870    2921    1870 
   Size:       1051    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2133    3725    2133 
   Size:       1592    4004
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3404     276 
   Size:         53      83     186     936    1870    2468

  Fragments arranged by size: 

                2468    1870     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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