################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII AccI AccIII AcyI AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AvaI AviII AvrII BalI BamHI BbeI BbrPI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsoBI BspDI BspEI BspHI BspLU11I BsrFI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DrdI EaeI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 1933 GGTACC G^GTACC AclI 1200 AACGTT AA^CGTT AflII 1923 CTTAAG C^TTAAG AocI 2002 CCTGAGG CC^T[ACGT]AGG Asp718I 1933 GGTACC G^GTACC AspI 874 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 194 CGAGCACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1923 CTTAAG C^TTAAG BsaI 760 GAGACC GGTCTC(1/5) BseRI 46 GAGGAG GAGGAG(10/8) BsiEI 581 CGACCG CG[GA][CT]^CG BsmBI 446 GAGACG CGTCTC(1/5) BsmI 1470 GCATTC GAATGC(1/-1) Bsp1407I 1316 TGTACA T^GTACA BspMI 979 ACCTGC ACCTGC(4/8) BsrDI 1207 GCAATG GCAATG(2/0) BsrGI 1316 TGTACA T^GTACA BssSI 851 CTCGTG CACGAG(-5/-1) BstAPI 1275 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2002 CCTGAGG CC^T[ACGT]AGG DraII 1493 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 521 CCATGG C^C[GA][CT]GG Eco81I 2002 CCTGAGG CC^T[ACGT]AGG EcoNI 1714 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1493 GGGACCT [GA]G^G[ACGT]CC[CT] KpnI 1933 GGTACC GGTAC^C MspA1I 58 CAGCGG C[AC]G^C[GT]G NcoI 521 CCATGG C^CATGG NdeI 1049 CATATG CA^TATG NspI 1441 GCATGT [GA]CATG^[CT] PacI 1813 TTAATTAA TTAAT^TAA PflFI 874 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1493 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 1200 AACGTT AA^CGTT SacI 1378 GAGCTC GAGCT^C SspBI 1316 TGTACA T^GTACA SspI 1340 AATATT AAT^ATT Tth111I 874 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 765 CCACTATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AhdI 448 GACGTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1663 GACTACTGGTC AlwNI 118 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 968 CAGAAGCTG AspEI 448 GACGTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1663 GACTACTGGTC BanI 4 GGCACC G^G[CT][GA]CC 1933 GGTACC BbsI 856 GAAGAC GAAGAC(2/6) 871 GAAGAC BbvCI 732 CCTCAGC CCTCAGC(-5/-2) 51 GCTGAGG BcnI 405 CCCGG CC^[GC]GG 572 CCCGG BfaI 1369 CTAG C^TAG 1519 CTAG BpuAI 856 GAAGAC GAAGAC(2/6) 871 GAAGAC BsrBI 147 CCGCTC CCGCTC(-3/-3) 1263 CCGCTC DraI 690 TTTAAA TTT^AAA 1006 TTTAAA DraIII 863 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG 1231 CACATTGTG Eam1105I 448 GACGTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1663 GACTACTGGTC EarI 724 GAAGAG CTCTTC(1/4) 757 GAAGAG FauI 12 GCGGG CCCGC(4/6) 60 GCGGG HapII 406 CCGG C^CGG 573 CCGG HpaII 406 CCGG C^CGG 573 CCGG Ksp632I 724 GAAGAG CTCTTC(1/4) 757 GAAGAG MaeI 1369 CTAG C^TAG 1519 CTAG MspI 406 CCGG C^CGG 573 CCGG NciI 405 CCCGG CC^[GC]GG 572 CCCGG PstI 124 CTGCAG CTGCA^G 965 CTGCAG SfcI 124 CTGCAG C^T[GA][CT]AG 965 CTGCAG TspRI 458 CACTG CA[GC]TG[ACGT][ACGT]^ 1673 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HindIII HinfI HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TthHB8I XhoII