(Circular) (Six-base) MAPSORT of: pFN21AB6261.seq Check: 7704 from: 1 to: 6763 pFN21AB6261 6763 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4573 278 Size: 53 83 186 936 3037 2468 Fragments arranged by size: 3037 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4106 1937 Size: 2169 4594 Acc65I G'GTAC_C Cuts at: 3938 4084 3938 Size: 146 6617 AclI AA'CG_TT Cuts at: 1563 3206 4890 5263 1563 Size: 1643 1684 373 3063 Fragments arranged by size: 3063 1684 1643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2748 3111 3134 4826 6210 6478 1632 Size: 1116 363 23 1692 1384 268 1917 Fragments arranged by size: 1917 1692 1384 1116 363 268 23 AflII C'TTAA_G Cuts at: 820 1017 3928 820 Size: 197 2911 3655 Fragments arranged by size: 3655 2911 197 AflIII A'CryG_T Cuts at: 1203 5662 6639 1203 Size: 4459 977 1327 Fragments arranged by size: 4459 1327 977 AgeI A'CCGG_T Cuts at: 5594 5594 Size: 6763 AhdI GACnn_n'nnGTC Cuts at: 2458 3673 5492 2458 Size: 1215 1819 3729 Fragments arranged by size: 3729 1819 1215 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6763 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2085 1184 Size: 901 5862 AlwNI CAG_nnn'CTG Cuts at: 2128 2978 4506 6078 2128 Size: 850 1528 1572 2813 Fragments arranged by size: 2813 1572 1528 850 ApaLI G'TGCA_C Cuts at: 4820 5976 4820 Size: 1156 5607 ApoI r'AATT_y Cuts at: 1493 2690 3782 3862 4072 4298 4450 6559 1493 Size: 1197 1092 80 210 226 152 2109 1697 Fragments arranged by size: 2109 1697 1197 1092 226 210 152 80 AseI AT'TA_AT Cuts at: 160 1032 5317 160 Size: 872 4285 1606 Fragments arranged by size: 4285 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6763 AvaI C'yCGr_G Cuts at: 1870 1943 4088 1870 Size: 73 2145 4545 Fragments arranged by size: 4545 2145 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2557 2590 2557 Size: 33 6730 BamHI G'GATC_C Cuts at: 4093 4460 4093 Size: 367 6396 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 3938 4084 5539 618 Size: 325 217 627 222 1929 146 1455 1842 Fragments arranged by size: 1929 1842 1455 627 325 222 217 146 BanII G_rGCy'C Cuts at: 1391 2253 2312 3298 3387 4082 1391 Size: 862 59 986 89 695 4072 Fragments arranged by size: 4072 986 862 695 89 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2868 2883 928 Size: 971 969 15 4808 Fragments arranged by size: 4808 971 969 15 BbvCI CC'TCA_GC Cuts at: 2057 2738 2057 Size: 681 6082 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2188 2222 4954 4988 1903 Size: 34 251 34 2732 34 3678 Fragments arranged by size: 3678 2732 251 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4655 5865 4655 Size: 1210 5553 BclI T'GATC_A Cuts at: 1548 1548 Size: 6763 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5374 136 Size: 107 122 71 631 605 3702 1525 Fragments arranged by size: 3702 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6758 6758 Size: 6763 BlpI GC'TnA_GC Cuts at: 4180 4180 Size: 6763 Bme1580I G_kGCm'C Cuts at: 1790 2012 4824 5980 1790 Size: 222 2812 1156 2573 Fragments arranged by size: 2812 2573 1156 222 BmgBI CAC'GTC Cuts at: 1318 2455 1318 Size: 1137 5626 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2876 3689 3928 Size: 416 53 83 399 24 1459 813 239 Cuts at: 3928 4494 5452 442 Size: 566 958 1753 Fragments arranged by size: 1753 1459 958 813 566 416 399 239 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6763 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2021 2108 2114 3597 5423 1239 Size: 782 87 6 1483 1826 2579 Fragments arranged by size: 2579 1826 1483 782 87 6 Bpu10I CC'TnA_GC Cuts at: 2057 2738 2057 Size: 681 6082 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3209 3488 4888 5753 6051 3209 Size: 279 1400 865 298 3921 Fragments arranged by size: 3921 1400 865 298 279 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2759 5426 882 Size: 617 1260 2667 2219 Fragments arranged by size: 2667 2219 1260 617 BsaAI yAC'GTr Cuts at: 493 1204 4554 493 Size: 711 3350 2702 Fragments arranged by size: 3350 2702 711 BsaBI GATnn'nnATC Cuts at: 6757 6757 Size: 6763 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4570 4952 5587 275 Size: 53 83 186 936 3037 382 635 1451 Fragments arranged by size: 3037 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5196 5594 5868 6015 6426 5196 Size: 398 274 147 411 5533 Fragments arranged by size: 5533 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 1178 Size: 30 165 30 678 30 5830 Fragments arranged by size: 5830 678 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 5883 BseYI C'CCAG_C Cuts at: 2175 5966 6462 2175 Size: 3791 496 2476 Fragments arranged by size: 3791 2476 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6190 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2589 4213 4974 6002 1540 Size: 275 186 588 1624 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 588 275 186 BsiHKAI G_wGCw'C Cuts at: 2204 3264 3387 4824 4909 5980 2204 Size: 1060 123 1437 85 1071 2987 Fragments arranged by size: 2987 1437 1071 1060 123 85 BsmI GAATG_Cn' Cuts at: 3475 4274 4367 3475 Size: 799 93 5871 Fragments arranged by size: 5871 799 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2445 2445 Size: 6763 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2204 2253 2312 3264 3298 3387 Size: 399 222 192 49 59 952 34 89 Cuts at: 3387 4082 4824 4909 5980 1391 Size: 695 742 85 1071 2174 Fragments arranged by size: 2174 1071 952 742 695 399 222 192 89 85 59 49 34 BspEI T'CCGG_A Cuts at: 6426 6426 Size: 6763 BspHI T'CATG_A Cuts at: 4652 6382 4652 Size: 1730 5033 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2993 4118 844 Size: 2149 1125 3489 Fragments arranged by size: 3489 2149 1125 BsrBI CCG'CTC Cuts at: 1394 1823 2154 3270 4650 1394 Size: 429 331 1116 1380 3507 Fragments arranged by size: 3507 1380 1116 429 331 BsrDI GCAATG_nn' Cuts at: 65 1248 3219 5258 5432 65 Size: 1183 1971 2039 174 1396 Fragments arranged by size: 2039 1971 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5407 5594 1700 Size: 110 3597 187 2869 Fragments arranged by size: 3597 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3321 96 Size: 3225 3538 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6763 BssSI C'ACGA_G Cuts at: 2856 4823 5835 2856 Size: 1967 1012 3784 Fragments arranged by size: 3784 1967 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3286 794 Size: 2492 4271 BstBI TT'CG_AA Cuts at: 5567 5567 Size: 6763 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6763 BstYI r'GATC_y Cuts at: 1972 2272 2323 2382 2733 3733 4093 4460 4845 Size: 300 51 59 351 1000 360 367 385 Cuts at: 4845 4862 6314 6400 6412 6758 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1000 385 367 360 351 346 300 86 59 51 17 12 Bsu36I CC'TnA_GG Cuts at: 4008 4008 Size: 6763 BtgI C'CryG_G Cuts at: 513 1065 2004 2526 513 Size: 552 939 522 4750 Fragments arranged by size: 4750 939 552 522 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6024 Fragments arranged by size: 6024 683 56 BtsI GCAGTG_nn' Cuts at: 4279 5073 5093 4279 Size: 794 20 5949 Fragments arranged by size: 5949 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6763 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6728 DraI TTT'AAA Cuts at: 2697 3013 4067 2697 Size: 316 1054 5393 Fragments arranged by size: 5393 1054 316 DraIII CAC_nnn'GTG Cuts at: 2873 3241 2873 Size: 368 6395 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5770 809 Size: 507 4454 1802 Fragments arranged by size: 4454 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4210 5099 6728 Size: 54 1425 50 275 18 2380 889 1629 Cuts at: 6728 8 Size: 43 Fragments arranged by size: 2380 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4210 1537 Size: 275 2398 4090 Fragments arranged by size: 4090 2398 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2724 2757 4693 1353 Size: 1371 33 1936 3423 Fragments arranged by size: 3423 1936 1371 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2233 5350 5724 5870 387 Size: 1064 138 644 3117 374 146 1280 Fragments arranged by size: 3117 1280 1064 644 374 146 138 EcoICRI GAG'CTC Cuts at: 3385 3385 Size: 6763 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2021 2108 2114 2748 3111 3134 3597 Size: 393 389 87 6 634 363 23 463 Cuts at: 3597 4826 5423 6210 6478 1239 Size: 1229 597 787 268 1524 Fragments arranged by size: 1524 1229 787 634 597 463 393 389 363 268 87 23 6 EcoNI CCTnn'n_nnAGG Cuts at: 3723 3723 Size: 6763 EcoO109I rG'GnC_Cy Cuts at: 3499 3499 Size: 6763 EcoRI G'AATT_C Cuts at: 4072 4450 4072 Size: 378 6385 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2360 2360 Size: 6763 FspI TGC'GCA Cuts at: 5269 5269 Size: 6763 HaeII r_GCGC'y Cuts at: 1678 5910 1678 Size: 4232 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 2990 3022 3659 Size: 32 135 32 872 32 514 32 637 Cuts at: 3659 3691 3830 3862 4025 4057 5404 5436 5478 Size: 32 139 32 163 32 1347 32 42 Cuts at: 5478 5510 1373 Size: 32 2626 Fragments arranged by size: 2626 1347 872 637 514 163 139 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4107 4353 669 Size: 988 281 2169 246 3079 Fragments arranged by size: 3079 2169 988 281 246 HindIII A'AGCT_T Cuts at: 748 2744 3366 3459 748 Size: 1996 622 93 4052 Fragments arranged by size: 4052 1996 622 93 HpaI GTT'AAC Cuts at: 4353 4353 Size: 6763 KpnI G_GTAC'C Cuts at: 3942 4088 3942 Size: 146 6617 MfeI C'AATT_G Cuts at: 4362 4362 Size: 6763 MluI A'CGCG_T Cuts at: 6639 6639 Size: 6763 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2079 2188 2732 3133 5877 6061 1305 Size: 774 109 544 401 2744 184 2007 Fragments arranged by size: 2744 2007 774 544 401 184 109 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4941 Fragments arranged by size: 4941 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4721 5080 5239 518 Size: 648 3555 359 159 2042 Fragments arranged by size: 3555 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2065 4179 4856 6004 6249 1606 Size: 459 2114 677 1148 245 2120 Fragments arranged by size: 2120 2114 1148 677 459 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2526 513 Size: 552 939 522 4750 Fragments arranged by size: 4750 939 552 522 NdeI CA'TA_TG Cuts at: 387 3055 387 Size: 2668 4095 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6763 NotI GC'GGCC_GC Cuts at: 4210 4210 Size: 6763 NspI r_CATG'y Cuts at: 1132 1229 3450 4121 5666 1132 Size: 97 2221 671 1545 2229 Fragments arranged by size: 2229 2221 1545 671 97 PacI TTA_AT'TAA Cuts at: 3822 3822 Size: 6763 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6763 PciI A'CATG_T Cuts at: 5662 5662 Size: 6763 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6763 PfoI T'CCnGG_A Cuts at: 2037 2268 2385 2533 2037 Size: 231 117 148 6267 Fragments arranged by size: 6267 231 148 117 PmeI GTTT'AAAC Cuts at: 4067 4067 Size: 6763 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4832 4864 6345 6377 4832 Size: 32 1481 32 5218 Fragments arranged by size: 5218 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3499 3499 Size: 6763 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6763 PsiI TTA'TAA Cuts at: 2330 4333 2330 Size: 2003 4760 PstI C_TGCA'G Cuts at: 830 2133 2974 4115 830 Size: 1303 841 1141 3478 Fragments arranged by size: 3478 1303 1141 841 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6763 SacI G_AGCT'C Cuts at: 3387 3387 Size: 6763 SalI G'TCGA_C Cuts at: 1936 4105 1936 Size: 2169 4594 SbfI CC_TGCA'GG Cuts at: 4115 4115 Size: 6763 ScaI AGT'ACT Cuts at: 5011 6723 5011 Size: 1712 5051 SfcI C'TryA_G Cuts at: 826 1045 2129 2970 4111 5246 5927 6118 826 Size: 219 1084 841 1141 1135 681 191 1471 Fragments arranged by size: 1471 1141 1135 1084 841 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6763 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6763 SmaI CCC'GGG Cuts at: 1872 4090 1872 Size: 2218 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3224 3503 3928 4867 5768 6030 Size: 197 926 1281 279 425 939 901 262 Cuts at: 6030 820 Size: 1553 Fragments arranged by size: 1553 1281 939 926 901 425 279 262 197 SnaBI TAC'GTA Cuts at: 493 4554 493 Size: 4061 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6763 SphI G_CATG'C Cuts at: 1132 4121 1132 Size: 2989 3774 SspI AAT'ATT Cuts at: 5 52 3347 4687 5 Size: 47 3295 1340 2081 Fragments arranged by size: 3295 2081 1340 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6763 StyI C'CwwG_G Cuts at: 513 1065 2004 2319 2526 3145 4202 513 Size: 552 939 315 207 619 1057 3074 Fragments arranged by size: 3074 1057 939 619 552 315 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 3991 4790 4807 4960 5145 5590 Size: 1255 609 1802 799 17 153 185 445 Cuts at: 5590 325 Size: 1498 Fragments arranged by size: 1802 1498 1255 799 609 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3321 5009 6721 Size: 274 80 33 51 1443 1344 1688 1712 Cuts at: 6721 96 Size: 138 Fragments arranged by size: 1712 1688 1443 1344 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3589 3621 3669 3701 6723 6755 3589 Size: 32 48 32 3022 32 3597 Fragments arranged by size: 3597 3022 48 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2882 1316 Size: 1566 5197 XbaI T'CTAG_A Cuts at: 4099 4099 Size: 6763 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2777 2777 Size: 6763 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6763 XmaI C'CCGG_G Cuts at: 1870 4088 1870 Size: 2218 4545 XmnI GAAnn'nnTTC Cuts at: 4892 4892 Size: 6763 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4571 276 Size: 53 83 186 936 3037 2468 Fragments arranged by size: 3037 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI