Restriction map for
pFN21AE3173
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI Asp718I
AspEI AspI AviII BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BpmI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1009 GACGTC GACGT^C
AccII 1146 CGCG CG^CG
AcyI 1009 GACGTC G[GA]^CG[CT]C
Aor51HI 1184 AGCGCT AGC^GCT
AseI 715 ATTAAT AT^TAAT
AsnI 715 ATTAAT AT^TAAT
AspHI 392 GAGCTC G[AT]GC[AT]^C
AvaII 23 GGTCC G^G[AT]CC
BalI 1203 TGGCCA TGG^CCA
BanI 916 GGCACC G^G[CT][GA]CC
BclI 324 TGATCA T^GATCA
BglII 1151 AGATCT A^GATCT
BlpI 111 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 111 GCTCAGC GC^T[ACGT]AGC
BsaHI 1009 GACGTC G[GA]^CG[CT]C
BsiHKAI 392 GAGCTC G[AT]GC[AT]^C
BspHI 840 TCATGA T^CATGA
BspMI 299 ACCTGC ACCTGC(4/8)
BsrFI 545 ACCGGC [GA]^CCGG[CT]
BstUI 1146 CGCG CG^CG
BstYI 1151 AGATCT [GA]^GATC[CT]
CelII 111 GCTCAGC GC^T[ACGT]AGC
Cfr10I 545 ACCGGC [GA]^CCGG[CT]
DraII 22 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 922 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1184 AGCGCT AGC^GCT
EcoO109I 22 GGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 324 TGATCA T^GATCA
HgaI 116 GCGTC GACGC(5/10)
Hin1I 1009 GACGTC G[GA]^CG[CT]C
HinfI 373 GACTC G^A[ACGT]TC
MflI 1151 AGATCT [GA]^GATC[CT]
MluNI 1203 TGGCCA TGG^CCA
MscI 1203 TGGCCA TGG^CCA
MslI 1087 CATTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1146 CGCG CG^CG
PleI 373 GACTC GAGTC(4/5)
PpuMI 22 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 817 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 715 ATTAAT AT^TAAT
PvuII 802 CAGCTG CAG^CTG
RcaI 840 TCATGA T^CATGA
SacI 392 GAGCTC GAGCT^C
Tsp45I 665 GTGAC ^GT[GC]AC
XhoII 1151 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 339 GAATTC [GA]^AATT[CT]
836 GAATTC
AlwI 997 GATCC GGATC(4/5)
512 GGATC
ApoI 339 GAATTC [GA]^AATT[CT]
836 GAATTC
Asp700I 60 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
756 GAAGGTCTTC
AvaI 949 CCCGGG C^[CT]CG[GA]G
985 CCCGGG
AvrII 18 CCTAGG C^CTAGG
1042 CCTAGG
BanII 392 GAGCTC G[GA]GC[CT]^C
639 GGGCTC
BlnI 18 CCTAGG C^CTAGG
1042 CCTAGG
BmyI 392 GAGCTC G[AGT]GC[ACT]^C
639 GGGCTC
BsoBI 949 CCCGGG C^[CT]CG[GA]G
985 CCCGGG
Bsp1286I 392 GAGCTC G[AGT]GC[ACT]^C
639 GGGCTC
DdeI 112 CTCAG C^T[ACGT]AG
154 CTGAG
EaeI 455 TGGCCG [CT]^GGCC[GA]
1203 TGGCCA
EcoRI 339 GAATTC G^AATTC
836 GAATTC
EcoT14I 18 CCTAGG C^C[AT][AT]GG
1042 CCTAGG
HaeII 1138 GGCGCT [GA]GCGC^[CT]
1184 AGCGCT
MaeII 452 ACGT A^CGT
1010 ACGT
MspA1I 106 CCGCTG C[AC]G^C[GT]G
802 CAGCTG
NspI 248 ACATGC [GA]CATG^[CT]
539 GCATGT
PflMI 528 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
921 CCACCCGGTGG
SfaNI 183 GATGC GCATC(5/9)
1093 GATGC
SfcI 644 CTACAG C^T[GA][CT]AG
819 CTACAG
SmaI 949 CCCGGG CCC^GGG
985 CCCGGG
StyI 18 CCTAGG C^C[AT][AT]GG
1042 CCTAGG
Van91I 528 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
921 CCACCCGGTGG
XcmI 378 CCAGGCCCGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
443 CCAGTGAAGACGTGG
XmaCI 949 CCCGGG C^CCGGG
985 CCCGGG
XmaI 949 CCCGGG C^CCGGG
985 CCCGGG
XmnI 60 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
756 GAAGGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI BbsI BbvI
BcnI BfaI BpuAI BsaJI BseRI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I