Restriction map for
pFN21AE1629
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HindIII HpaI KasI KpnI Ksp632I
MaeI MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NarI NciI NdeI NheI
NotI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I TspRI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 358 GACGCC G[GA]^CG[CT]C
AfaI 36 GTAC GT^AC
AlwI 366 GATCC GGATC(4/5)
AocI 19 CCTGAGG CC^T[ACGT]AGG
AspHI 304 GAGCTC G[AT]GC[AT]^C
BanI 76 GGCACC G^G[CT][GA]CC
BanII 304 GAGCTC G[GA]GC[CT]^C
BmyI 304 GAGCTC G[AGT]GC[ACT]^C
BpmI 238 CTGGAG CTGGAG(16/14)
BsaHI 358 GACGCC G[GA]^CG[CT]C
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
Bsp1286I 304 GAGCTC G[AGT]GC[ACT]^C
BsrI 33 CCAGT ACTGG(1/-1)
BstAPI 333 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 19 CCTGAGG CC^T[ACGT]AGG
DpnII 366 GATC ^GATC
DraII 242 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 244 GACCTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 19 CCTGAGG CC^T[ACGT]AGG
EcoNI 129 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 242 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 317 CCATGG C^C[AT][AT]GG
FseI 231 GGCCGGCC GGCCGG^CC
HaeII 105 GGCGCT [GA]GCGC^[CT]
HgaI 358 GACGC GACGC(5/10)
Hin1I 358 GACGCC G[GA]^CG[CT]C
HinfI 15 GACTC G^A[ACGT]TC
KspI 53 CCGCGG CCGC^GG
MaeII 380 ACGT A^CGT
MboI 366 GATC ^GATC
MboII 219 GAAGA GAAGA(8/7)
MslI 291 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 232 GCCGGC GCC^GGC
NcoI 317 CCATGG C^CATGG
NdeII 366 GATC ^GATC
NgoMIV 232 GCCGGC G^CCGGC
NlaIV 76 GGCACC GG[ACGT]^[ACGT]CC
NruI 416 TCGCGA TCG^CGA
PflMI 90 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 15 GACTC GAGTC(4/5)
PpuMI 242 AGGACCT [GA]G^G[AT]CC[CT]
PstI 247 CTGCAG CTGCA^G
RsaI 36 GTAC GT^AC
SacI 304 GAGCTC GAGCT^C
SacII 53 CCGCGG CCGC^GG
Sau3AI 366 GATC ^GATC
SbfI 246 CCTGCAGG CCTGCA^GG
SfaNI 423 GATGC GCATC(5/9)
SfcI 247 CTGCAG C^T[GA][CT]AG
Sse8387I 246 CCTGCAGG CCTGCA^GG
StyI 317 CCATGG C^C[AT][AT]GG
TaqI 269 TCGA T^CGA
TthHB8I 269 TCGA T^CGA
Van91I 90 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 91 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
334 CAGCCGCTG
AvaII 243 GGACC G^G[AT]CC
384 GGACC
BbvI 333 GCAGC GCAGC(8/12)
339 GCTGC
BspMI 245 ACCTGC ACCTGC(4/8)
249 GCAGGT
BsrFI 4 GCCGGT [GA]^CCGG[CT]
232 GCCGGC
Cfr10I 4 GCCGGT [GA]^CCGG[CT]
232 GCCGGC
EaeI 2 TGGCCG [CT]^GGCC[GA]
285 CGGCCA
HapII 5 CCGG C^CGG
233 CCGG
HincII 58 GTTGAC GT[CT]^[GA]AC
166 GTTGAC
HindII 58 GTTGAC GT[CT]^[GA]AC
166 GTTGAC
HpaII 5 CCGG C^CGG
233 CCGG
MspA1I 53 CCGCGG C[AC]G^C[GT]G
337 CCGCTG
MspI 5 CCGG C^CGG
233 CCGG
NlaIII 318 CATG CATG^
363 CATG
TseI 333 GCAGC G^C[AT]GC
339 GCTGC
XcmI 227 CCAGGGCCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
317 CCATGGCGGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AccII AciI AluI BsaJI BseRI
BsiYI BslI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DsaI EcoRII FauI Fnu4HI FokI
HaeIII HhaI HinP1I HphI ItaI
MnlI MvaI MvnI MwoI Sau96I
ScrFI