Restriction map for pFN21AE1629
 (Circular) (Six-base) MAPSORT of: pFN21AE1629.seq  Check: 2229  from: 1  to: 5143



pFN21AE1629  5143 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:53  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2953     278 
   Size:         53      83     186     936    1417    2468

  Fragments arranged by size: 

                2468    1417     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2486    1937 
   Size:        549    4594
 
Acc65I G'GTAC_C

Cuts at:   2464    2464 
   Size:       5143
 
AclI AA'CG_TT

Cuts at:   1563    3270    3643    1563 
   Size:       1707     373    3063

  Fragments arranged by size: 

                3063    1707     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2135    3206    4590    4858    1632 
   Size:        503    1071    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1071     503     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4946
 
AflIII A'CryG_T

Cuts at:   1203    4042    5019    1203 
   Size:       2839     977    1327

  Fragments arranged by size: 

                2839    1327     977
 
AgeI A'CCGG_T

Cuts at:   3974    3974 
   Size:       5143
 
AhdI GACnn_n'nnGTC

Cuts at:   3872    3872 
   Size:       5143
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5143
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5143
 
AlwNI CAG_nnn'CTG

Cuts at:   2101    2344    2886    4458    2101 
   Size:        243     542    1572    2786

  Fragments arranged by size: 

                2786    1572     542     243
 
ApaLI G'TGCA_C

Cuts at:   3200    4356    3200 
   Size:       1156    3987
 
ApoI r'AATT_y

Cuts at:   1493    2452    2678    2830    4939    1493 
   Size:        959     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     959     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3697     160 
   Size:        872    2665    1606

  Fragments arranged by size: 

                2665    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5143
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2468    1870 
   Size:         73     525    4545

  Fragments arranged by size: 

                4545     525      73
 
BamHI G'GATC_C

Cuts at:   2473    2840    2473 
   Size:        367    4776
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2081    2464    3919     618 
   Size:        325     217     627     294     383    1455    1842

  Fragments arranged by size: 

                1842    1455     627     383     325     294     217
 
BanII G_rGCy'C

Cuts at:   1391    2313    2462    1391 
   Size:        922     149    4072

  Fragments arranged by size: 

                4072     922     149
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4172
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3334    3368    1903 
   Size:         34    1397      34    3678

  Fragments arranged by size: 

                3678    1397      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3035    4245    3035 
   Size:       1210    3933
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5143
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3754     136 
   Size:        107     122      71     631     605    2082    1525

  Fragments arranged by size: 

                2082    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5138    5138 
   Size:       5143
 
BlpI GC'TnA_GC

Cuts at:   2560    2560 
   Size:       5143
 
Bme1580I G_kGCm'C

Cuts at:   1790    3204    4360    1790 
   Size:       1414    1156    2573

  Fragments arranged by size: 

                2573    1414    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2386    1318 
   Size:       1068    4075
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2032    2874    3832 
   Size:        416      53      83     399      24     615     842     958

Cuts at:   3832     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     842     615     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5143
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2264    3803    1239 
   Size:       1025    1539    2579

  Fragments arranged by size: 

                2579    1539    1025
 
Bpu10I CC'TnA_GC

Cuts at:   2150    2312    2150 
   Size:        162    4981
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3268    4133    4431    3268 
   Size:        865     298    3980

  Fragments arranged by size: 

                3980     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3806     882 
   Size:        617    2307    2219

  Fragments arranged by size: 

                2307    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2934     493 
   Size:        711    1730    2702

  Fragments arranged by size: 

                2702    1730     711
 
BsaBI GATnn'nnATC

Cuts at:   5137    5137 
   Size:       5143
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2364    2950    3332    3967 
   Size:         53      83     186     936     831     586     382     635

Cuts at:   3967     275 
   Size:       1451

  Fragments arranged by size: 

                1451     936     831     635     586     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3576    3974    4248    4395    4806    3576 
   Size:        398     274     147     411    3913

  Fragments arranged by size: 

                3913     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2024    2054    1178 
   Size:         30     165      30     621      30    4267

  Fragments arranged by size: 

                4267     621     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2042    2045    2369    2429    1186 
   Size:        856       3     324      60    3900

  Fragments arranged by size: 

                3900     856     324      60       3
 
BseYI C'CCAG_C

Cuts at:   2101    2145    2332    4346    4842    2101 
   Size:         44     187    2014     496    2402

  Fragments arranged by size: 

                2402    2014     496     187      44
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4570
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2593    3354    4382    1540 
   Size:        275     186     592     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     592     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2313    3204    3289    4360    2313 
   Size:        891      85    1071    3096

  Fragments arranged by size: 

                3096    1071     891      85
 
BsmI GAATG_Cn'

Cuts at:   2654    2747    2654 
   Size:         93    5050
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2313    2462    3204    3289    4360    1391 
   Size:        399     523     149     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     523     399     149      85
 
BspEI T'CCGG_A

Cuts at:   4806    4806 
   Size:       5143
 
BspHI T'CATG_A

Cuts at:   3032    4762    3032 
   Size:       1730    3413
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2245    2259    2498     844 
   Size:       1401      14     239    3489

  Fragments arranged by size: 

                3489    1401     239      14
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3030    1394 
   Size:        429    1207    3507

  Fragments arranged by size: 

                3507    1207     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3638    3812      65 
   Size:       1183    2390     174    1396

  Fragments arranged by size: 

                2390    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2009    2237    3787    3974    1700 
   Size:        110     199     228    1550     187    2869

  Fragments arranged by size: 

                2869    1550     228     199     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5143
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5143
 
BssSI C'ACGA_G

Cuts at:   3203    4215    3203 
   Size:       1012    4131
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2344     794 
   Size:       1550    3593
 
BstBI TT'CG_AA

Cuts at:   3947    3947 
   Size:       5143
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5143
 
BstYI r'GATC_y

Cuts at:   1972    2473    2840    3225    3242    4694    4780    4792    5138 
   Size:        501     367     385      17    1452      86      12     346

Cuts at:   5138    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     501     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2025    2025 
   Size:       5143
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2058    2121    2301    2322     513 
   Size:        552     939      54      63     180      21    3334

  Fragments arranged by size: 

                3334     939     552     180      63      54      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4404

  Fragments arranged by size: 

                4404     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2659    3453    3473    2659 
   Size:        794      20    4329

  Fragments arranged by size: 

                4329     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5143
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5108
 
DraI TTT'AAA

Cuts at:   2447    2447 
   Size:       5143
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2255    4150     809 
   Size:        507     939    1895    1802

  Fragments arranged by size: 

                1895    1802     939     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2007    2290    2590 
   Size:         54    1425      50     275      18     177     283     300

Cuts at:   2590    3479    5108       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     300     283     275     177      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2590    1537 
   Size:        275     778    4090

  Fragments arranged by size: 

                4090     778     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3073    1353 
   Size:       1720    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3730    4104    4250     387 
   Size:       1064     138    2141     374     146    1280

  Fragments arranged by size: 

                2141    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2311    2311 
   Size:       5143
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2135    2264    3206    3803    4590    4858    1239 
   Size:        393     503     129     942     597     787     268    1524

  Fragments arranged by size: 

                1524     942     787     597     503     393     268     129
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2138    2138 
   Size:       5143
 
EcoO109I rG'GnC_Cy

Cuts at:   2248    2248 
   Size:       5143
 
EcoRI G'AATT_C

Cuts at:   2452    2830    2452 
   Size:        378    4765
 
FseI GG_CCGG'CC

Cuts at:   2241    2241 
   Size:       5143
 
FspI TGC'GCA

Cuts at:   3649    3649 
   Size:       5143
 
HaeII r_GCGC'y

Cuts at:   1678    2114    4290    1678 
   Size:        436    2176    2531

  Fragments arranged by size: 

                2531    2176     436
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3784    3816    3858    3890    1373 
   Size:         32     135      32    2212      32      42      32    2626

  Fragments arranged by size: 

                2626    2212     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2065    2173    2487    2733     669 
   Size:        988     281     127     108     314     246    3079

  Fragments arranged by size: 

                3079     988     314     281     246     127     108
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5143
 
HpaI GTT'AAC

Cuts at:   2733    2733 
   Size:       5143
 
KpnI G_GTAC'C

Cuts at:   2468    2468 
   Size:       5143
 
MfeI C'AATT_G

Cuts at:   2742    2742 
   Size:       5143
 
MluI A'CGCG_T

Cuts at:   5019    5019 
   Size:       5143
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4257    4441    1305 
   Size:       2952     184    2007

  Fragments arranged by size: 

                2952    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3321

  Fragments arranged by size: 

                3321    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2300    3101    3460    3619     518 
   Size:        648    1134     801     359     159    2042

  Fragments arranged by size: 

                2042    1134     801     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2060    2344    2559    3236    4384    4629    1606 
   Size:        454     284     215     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     454     284     245     215
 
NaeI GCC'GGC

Cuts at:   2239    2239 
   Size:       5143
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2322     513 
   Size:        552     939     318    3334

  Fragments arranged by size: 

                3334     939     552     318
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5143
 
NgoMIV G'CCGG_C

Cuts at:   2237    2237 
   Size:       5143
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5143
 
NotI GC'GGCC_GC

Cuts at:   2590    2590 
   Size:       5143
 
NruI TCG'CGA

Cuts at:   2423    2423 
   Size:       5143
 
NspI r_CATG'y

Cuts at:   1132    1229    2501    4046    1132 
   Size:         97    1272    1545    2229

  Fragments arranged by size: 

                2229    1545    1272      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5143
 
PciI A'CATG_T

Cuts at:   4042    4042 
   Size:       5143
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2101    1108 
   Size:        993    4150
 
PfoI T'CCnGG_A

Cuts at:   2202    2202 
   Size:       5143
 
PmeI GTTT'AAAC

Cuts at:   2447    2447 
   Size:       5143
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3212    3244    4725    4757    3212 
   Size:         32    1481      32    3598

  Fragments arranged by size: 

                3598    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2248    2248 
   Size:       5143
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5143
 
PsiI TTA'TAA

Cuts at:   2713    2713 
   Size:       5143
 
PstI C_TGCA'G

Cuts at:    830    2256    2495     830 
   Size:       1426     239    3478

  Fragments arranged by size: 

                3478    1426     239
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5143
 
SacI G_AGCT'C

Cuts at:   2313    2313 
   Size:       5143
 
SacII CC_GC'GG

Cuts at:   2061    2061 
   Size:       5143
 
SalI G'TCGA_C

Cuts at:   1936    2485    1936 
   Size:        549    4594
 
SbfI CC_TGCA'GG

Cuts at:   2256    2495    2256 
   Size:        239    4904
 
ScaI AGT'ACT

Cuts at:   3391    5103    3391 
   Size:       1712    3431
 
SfcI C'TryA_G

Cuts at:    826    1045    2252    2491    3626    4307    4498     826 
   Size:        219    1207     239    1135     681     191    1471

  Fragments arranged by size: 

                1471    1207    1135     681     239     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5143
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5143
 
SmaI CCC'GGG

Cuts at:   1872    2470    1872 
   Size:        598    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3247    4148    4410     820 
   Size:        197     926    1304     901     262    1553

  Fragments arranged by size: 

                1553    1304     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2934     493 
   Size:       2441    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5143
 
SphI G_CATG'C

Cuts at:   1132    2501    1132 
   Size:       1369    3774
 
SspI AAT'ATT

Cuts at:      5      52    3067       5 
   Size:         47    3015    2081

  Fragments arranged by size: 

                3015    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5143
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2322    2582     513 
   Size:        552     939     318     260    3074

  Fragments arranged by size: 

                3074     939     552     318     260
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3170    3187    3340    3525    3970     325 
   Size:       1255    1590      17     153     185     445    1498

  Fragments arranged by size: 

                1590    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2040    3389    5101 
   Size:        274      80      33      51    1443      63    1349    1712

Cuts at:   5101      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1349     274     138      80      63      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5103    5135    5103 
   Size:         32    5111
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5143
 
XbaI T'CTAG_A

Cuts at:   2479    2479 
   Size:       5143
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2239    2329    2239 
   Size:         90    5053
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5143
 
XmaI C'CCGG_G

Cuts at:   1870    2468    1870 
   Size:        598    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3272    3272 
   Size:       5143
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2951     276 
   Size:         53      83     186     936    1417    2468

  Fragments arranged by size: 

                2468    1417     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FseI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI     SacI    SacII
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   DraIII
    EcoRV     FalI    FspAI     KasI     NarI     NsiI     PacI     PmlI
   PspOMI    PspXI     PsrI    PvuII    RsrII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI
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