################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BseRI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MflI MroI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SfcI SfiI SfuI SgrAI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AcyI 96 GGCGCC G[GA]^CG[CT]C Alw44I 361 GTGCAC G^TGCAC AlwI 699 GATCC GGATC(4/5) AlwNI 614 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 782 AGCGCT AGC^GCT ApaLI 361 GTGCAC G^TGCAC AvaII 169 GGACC G^G[AT]CC BalI 191 TGGCCA TGG^CCA BanII 727 GAGCTC G[GA]GC[CT]^C BbeI 96 GGCGCC GGCGC^C BbrPI 664 CACGTG CAC^GTG BcgI 401 GCAGTGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 871 GCCATCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 96 GGCGCC G[GA]^CG[CT]C BsgI 585 CTGCAC GTGCAG(16/14) BsiWI 239 CGTACG C^GTACG BsmAI 308 GTCTC GTCTC(1/5) BsmBI 307 CGTCTC CGTCTC(1/5) BsmI 519 GAATGC GAATGC(1/-1) BspMI 712 GCAGGT ACCTGC(4/8) BsrBI 811 GAGCGG CCGCTC(-3/-3) BsrI 777 ACTGG ACTGG(1/-1) BssHII 272 GCGCGC G^CGCGC BstEII 418 GGTCACC G^GT[ACGT]ACC BstPI 418 GGTCACC G^GT[ACGT]ACC BstXI 770 CCATCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 481 CTGAG C^T[ACGT]AG DpnII 699 GATC ^GATC DraII 168 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 299 GACCCGAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 598 CTCTTC CTCTTC(1/4) Eco47III 782 AGCGCT AGC^GCT EcoO109I 168 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 418 GGTCACC G^GT[ACGT]ACC EcoT14I 461 CCATGG C^C[AT][AT]GG FseI 15 GGCCGGCC GGCCGG^CC Hin1I 96 GGCGCC G[GA]^CG[CT]C HphI 420 TCACC GGTGA(8/7) KasI 96 GGCGCC G^GCGCC Ksp632I 598 CTCTTC CTCTTC(1/4) MaeIII 419 GTCAC ^GT[ACGT]AC MboI 699 GATC ^GATC MluI 365 ACGCGT A^CGCGT MluNI 191 TGGCCA TGG^CCA MscI 191 TGGCCA TGG^CCA MslI 735 CACCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 96 GGCGCC GG^CGCC NcoI 461 CCATGG C^CATGG NdeII 699 GATC ^GATC NlaIII 462 CATG CATG^ PmaCI 664 CACGTG CAC^GTG PmlI 664 CACGTG CAC^GTG PpuMI 168 GGGACCC [GA]G^G[AT]CC[CT] PshAI 412 GACCGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 292 CAGCTG CAG^CTG SacI 727 GAGCTC GAGCT^C Sau3AI 699 GATC ^GATC SexAI 644 ACCAGGT A^CC[AT]GGT SfaNI 744 GATGC GCATC(5/9) SfoI 96 GGCGCC GGC^GCC SnaBI 634 TACGTA TAC^GTA StyI 461 CCATGG C^C[AT][AT]GG Tsp45I 419 GTCAC ^GT[GC]AC Tsp509I 723 AATT ^AATT XcmI 749 CCAACGCACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AflIII 365 ACGCGT A^C[GA][CT]GT 470 ACACGT AspHI 361 GTGCAC G[AT]GC[AT]^C 727 GAGCTC BsiHKAI 361 GTGCAC G[AT]GC[AT]^C 727 GAGCTC BsmFI 102 GGGAC GGGAC(10/14) 168 GGGAC EaeI 191 TGGCCA [CT]^GGCC[GA] 425 TGGCCG HgaI 85 GACGC GACGC(5/10) 306 GCGTC HinfI 235 GAGTC G^A[ACGT]TC 355 GACTC MboII 599 TCTTC GAAGA(8/7) 686 TCTTC NaeI 16 GCCGGC GCC^GGC 113 GCCGGC NgoMIV 16 GCCGGC G^CCGGC 113 GCCGGC PleI 355 GACTC GAGTC(4/5) 235 GAGTC SmaI 163 CCCGGG CCC^GGG 180 CCCGGG SmlI 223 CTCAAG C^T[CT][GA]AG 865 CTCAAG XmaCI 163 CCCGGG C^CCGGG 180 CCCGGG XmaI 163 CCCGGG C^CCGGG 180 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AfaI AluI AvaI BanI BbvI BcnI BmyI BpmI BsaAI BsaJI BsiEI BsiYI BslI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DsaI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI KspI MaeII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV RsaI SacII Sau96I ScrFI TaqI TseI TspRI TthHB8I