################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 409 AAATTC [GA]^AATT[CT] ApaI 179 GGGCCC GGGCC^C ApoI 409 AAATTC [GA]^AATT[CT] AspHI 58 GAGCAC G[AT]GC[AT]^C AspI 591 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 590 GGACC G^G[AT]CC BcnI 177 CCGGG CC^[GC]GG BfaI 367 CTAG C^TAG BseRI 580 CTCCTC GAGGAG(10/8) BsiHKAI 58 GAGCAC G[AT]GC[AT]^C BstF5I 470 GGATG GGATG(2/0) BstXI 527 CCATCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 9 GGATCT [GA]^GATC[CT] CfoI 95 GCGC GCG^C DraI 328 TTTAAA TTT^AAA DsaI 465 CCGTGG C^C[GA][CT]GG EaeI 2 TGGCCG [CT]^GGCC[GA] FokI 470 GGATG GGATG(9/13) HapII 177 CCGG C^CGG HhaI 95 GCGC GCG^C HinP1I 95 GCGC G^CGC HpaII 177 CCGG C^CGG MaeI 367 CTAG C^TAG MaeII 686 ACGT A^CGT MboII 721 GAAGA GAAGA(8/7) MflI 9 GGATCT [GA]^GATC[CT] MseI 329 TTAA T^TAA MslI 528 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 66 CAGCTG C[AC]G^C[GT]G MspI 177 CCGG C^CGG NciI 177 CCGGG CC^[GC]GG PflFI 591 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 69 CTGCAG CTGCA^G PvuII 66 CAGCTG CAG^CTG SfcI 69 CTGCAG C^T[GA][CT]AG Tru9I 329 TTAA T^TAA Tsp509I 410 AATT ^AATT Tth111I 591 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 343 CCAGCTCTGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 9 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AlwI 420 GATCC GGATC(4/5) 9 GGATC BanII 104 GGGCTC G[GA]GC[CT]^C 179 GGGCCC BsmAI 306 GAGAC GTCTC(1/5) 434 GAGAC BspMI 112 ACCTGC ACCTGC(4/8) 158 ACCTGC DpnII 10 GATC ^GATC 420 GATC MboI 10 GATC ^GATC 420 GATC NdeII 10 GATC ^GATC 420 GATC Sau3AI 10 GATC ^GATC 420 GATC StuI 191 AGGCCT AGG^CCT 369 AGGCCT Tsp45I 457 GTCAC ^GT[GC]AC 692 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AluI BbvI BmyI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI EcoRII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI MaeIII MnlI MvaI MwoI NlaIII NlaIV PleI Sau96I ScrFI StyI TseI TspRI