################################## # NO CUT ENZYMES ( 207 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BfrI BglI BglII BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI Fnu4HI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaI HphI ItaI KasI KpnI KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 250 GAATTT [GA]^AATT[CT] AlwI 157 GATCC GGATC(4/5) ApoI 250 GAATTT [GA]^AATT[CT] AvaII 72 GGACC G^G[AT]CC BclI 179 TGATCA T^GATCA BcnI 168 CCGGG CC^[GC]GG BfaI 25 CTAG C^TAG BlpI 147 GCTTAGC GC^T[ACGT]AGC BpmI 76 CTGGAG CTGGAG(16/14) Bpu1102I 147 GCTTAGC GC^T[ACGT]AGC BsaI 79 GAGACC GGTCTC(1/5) BsiYI 161 CCAAGAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 161 CCAAGAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 6 GAGACG CGTCTC(1/5) BstBI 65 TTCGAA TT^CGAA BstNI 75 CCTGG CC^[AT]GG CelII 147 GCTTAGC GC^T[ACGT]AGC Cfr13I 72 GGACC G^G[ACGT]CC DraII 71 AGGACCT [GA]G^G[ACGT]CC[CT] EarI 49 GAAGAG CTCTTC(1/4) EcoO109I 71 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 75 CCTGG ^CC[AT]GG FbaI 179 TGATCA T^GATCA HapII 168 CCGG C^CGG HindIII 34 AAGCTT A^AGCTT HpaII 168 CCGG C^CGG Ksp632I 49 GAAGAG CTCTTC(1/4) MaeI 25 CTAG C^TAG MnlI 119 GAGG CCTC(7/6) MspI 168 CCGG C^CGG MvaI 75 CCTGG CC^[AT]GG NciI 168 CCGGG CC^[GC]GG NlaIV 136 GGAGCC GG[ACGT]^[ACGT]CC NspV 65 TTCGAA TT^CGAA PpuMI 71 AGGACCT [GA]G^G[AT]CC[CT] Sau96I 72 GGACC G^G[ACGT]CC SfuI 65 TTCGAA TT^CGAA TaqI 66 TCGA T^CGA TthHB8I 66 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AluI 29 AGCT AG^CT 35 AGCT BsmAI 6 GAGAC GTCTC(1/5) 79 GAGAC BssKI 75 CCTGG ^CC[ACGT]GG 168 CCGGG DdeI 4 CTGAG C^T[ACGT]AG 148 CTTAG MboII 49 GAAGA GAAGA(8/7) 100 GAAGA ScrFI 75 CCTGG CC^[ACGT]GG 168 CCGGG Tsp509I 236 AATT ^AATT 251 AATT TspRI 123 CAGTG CA[GC]TG[ACGT][ACGT]^ 153 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### DpnII MboI NdeII Sau3AI