(Circular) (Six-base) MAPSORT of: pFN21AE1943.seq Check: 5644 from: 1 to: 6199 pFN21AE1943 6199 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4009 278 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2072 3542 1937 Size: 135 1470 4594 Fragments arranged by size: 4594 1470 135 Acc65I G'GTAC_C Cuts at: 3520 3520 Size: 6199 AclI AA'CG_TT Cuts at: 1563 4326 4699 1563 Size: 2763 373 3063 Fragments arranged by size: 3063 2763 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4262 5646 5914 1632 Size: 2630 1384 268 1917 Fragments arranged by size: 2630 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6002 AflIII A'CryG_T Cuts at: 1203 2145 5098 6075 1203 Size: 942 2953 977 1327 Fragments arranged by size: 2953 1327 977 942 AgeI A'CCGG_T Cuts at: 5030 5030 Size: 6199 AhdI GACnn_n'nnGTC Cuts at: 4928 4928 Size: 6199 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6199 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2959 1184 Size: 1775 4424 AlwNI CAG_nnn'CTG Cuts at: 3042 3942 5514 3042 Size: 900 1572 3727 Fragments arranged by size: 3727 1572 900 ApaLI G'TGCA_C Cuts at: 4256 5412 4256 Size: 1156 5043 ApoI r'AATT_y Cuts at: 1493 2897 3508 3734 3886 5995 1493 Size: 1404 611 226 152 2109 1697 Fragments arranged by size: 2109 1697 1404 611 226 152 AseI AT'TA_AT Cuts at: 160 1032 2468 4753 160 Size: 872 1436 2285 1606 Fragments arranged by size: 2285 1606 1436 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 AvaI C'yCGr_G Cuts at: 1870 1943 3524 1870 Size: 73 1581 4545 Fragments arranged by size: 4545 1581 73 BamHI G'GATC_C Cuts at: 3529 3896 3529 Size: 367 5832 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2927 3520 4975 618 Size: 325 217 627 1140 593 1455 1842 Fragments arranged by size: 1842 1455 1140 627 593 325 217 BanII G_rGCy'C Cuts at: 1391 3518 1391 Size: 2127 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3322 928 Size: 971 1423 3805 Fragments arranged by size: 3805 1423 971 BbvCI CC'TCA_GC Cuts at: 2922 3069 2922 Size: 147 6052 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3008 3042 4390 4424 1903 Size: 34 1071 34 1348 34 3678 Fragments arranged by size: 3678 1348 1071 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4091 5301 4091 Size: 1210 4989 BclI T'GATC_A Cuts at: 1548 2377 1548 Size: 829 5370 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4810 136 Size: 107 122 71 631 605 3138 1525 Fragments arranged by size: 3138 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3434 6194 3434 Size: 2760 3439 BlpI GC'TnA_GC Cuts at: 3616 3616 Size: 6199 Bme1580I G_kGCm'C Cuts at: 1790 2972 4260 5416 1790 Size: 1182 1288 1156 2573 Fragments arranged by size: 2573 1288 1182 1156 BmgBI CAC'GTC Cuts at: 1318 2146 1318 Size: 828 5371 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3930 4888 442 Size: 416 53 83 399 24 2513 958 1753 Fragments arranged by size: 2513 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4859 1239 Size: 3620 2579 Bpu10I CC'TnA_GC Cuts at: 2922 3069 2922 Size: 147 6052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4324 5189 5487 4324 Size: 865 298 5036 Fragments arranged by size: 5036 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3014 4862 882 Size: 617 1515 1848 2219 Fragments arranged by size: 2219 1848 1515 617 BsaAI yAC'GTr Cuts at: 493 1204 3990 493 Size: 711 2786 2702 Fragments arranged by size: 2786 2702 711 BsaBI GATnn'nnATC Cuts at: 6193 6193 Size: 6199 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4006 4388 5023 275 Size: 53 83 186 936 2473 382 635 1451 Fragments arranged by size: 2473 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4632 5030 5304 5451 5862 4632 Size: 398 274 147 411 4969 Fragments arranged by size: 4969 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5974 Fragments arranged by size: 5974 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6199 BseYI C'CCAG_C Cuts at: 2034 5402 5898 2034 Size: 3368 496 2335 Fragments arranged by size: 3368 2335 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3429 1163 Size: 573 1693 3933 Fragments arranged by size: 3933 1693 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2030 2167 3649 4410 5438 1540 Size: 275 186 29 137 1482 761 1028 2301 Fragments arranged by size: 2301 1482 1028 761 275 186 137 29 BsiHKAI G_wGCw'C Cuts at: 4260 4345 5416 4260 Size: 85 1071 5043 Fragments arranged by size: 5043 1071 85 BsmI GAATG_Cn' Cuts at: 3710 3803 3710 Size: 93 6106 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2972 3518 4260 4345 5416 1391 Size: 399 1182 546 742 85 1071 2174 Fragments arranged by size: 2174 1182 1071 742 546 399 85 BspEI T'CCGG_A Cuts at: 5862 5862 Size: 6199 BspHI T'CATG_A Cuts at: 2991 4088 5818 2991 Size: 1097 1730 3372 Fragments arranged by size: 3372 1730 1097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3554 844 Size: 2710 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2023 4086 1394 Size: 429 200 2063 3507 Fragments arranged by size: 3507 2063 429 200 BsrDI GCAATG_nn' Cuts at: 65 1248 2295 2577 2931 2941 3024 4694 4868 Size: 1183 1047 282 354 10 83 1670 174 Cuts at: 4868 65 Size: 1396 Fragments arranged by size: 1670 1396 1183 1047 354 282 174 83 10 BsrFI r'CCGG_y Cuts at: 1700 1810 4843 5030 1700 Size: 110 3033 187 2869 Fragments arranged by size: 3033 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2215 96 Size: 2119 4080 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6199 BssSI C'ACGA_G Cuts at: 4259 5271 4259 Size: 1012 5187 BstAPI GCAn_nnn'nTGC Cuts at: 794 2399 794 Size: 1605 4594 BstBI TT'CG_AA Cuts at: 5003 5003 Size: 6199 BstEII G'GTnAC_C Cuts at: 1184 2720 1184 Size: 1536 4663 BstYI r'GATC_y Cuts at: 1972 2094 3039 3434 3529 3896 4281 4298 5750 Size: 122 945 395 95 367 385 17 1452 Cuts at: 5750 5836 5848 6194 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 945 395 385 367 346 122 95 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5647 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5460 Fragments arranged by size: 5460 683 56 BtsI GCAGTG_nn' Cuts at: 3412 3460 3715 4509 4529 3412 Size: 48 255 794 20 5082 Fragments arranged by size: 5082 794 255 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6199 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6164 DraI TTT'AAA Cuts at: 2652 3503 2652 Size: 851 5348 DraIII CAC_nnn'GTG Cuts at: 3287 3287 Size: 6199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5206 809 Size: 507 3890 1802 Fragments arranged by size: 3890 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3646 4535 6164 Size: 54 1425 50 275 18 1816 889 1629 Cuts at: 6164 8 Size: 43 Fragments arranged by size: 1816 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3646 1537 Size: 275 1834 4090 Fragments arranged by size: 4090 1834 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4129 1353 Size: 2776 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4786 5160 5306 387 Size: 1064 138 3197 374 146 1280 Fragments arranged by size: 3197 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4262 4859 5646 5914 1239 Size: 393 2630 597 787 268 1524 Fragments arranged by size: 2630 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2131 2131 Size: 6199 EcoRI G'AATT_C Cuts at: 2897 3508 3886 2897 Size: 611 378 5210 Fragments arranged by size: 5210 611 378 EcoRV GAT'ATC Cuts at: 2908 2908 Size: 6199 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2972 2972 Size: 6199 FspI TGC'GCA Cuts at: 4705 4705 Size: 6199 HaeII r_GCGC'y Cuts at: 1678 2065 5346 1678 Size: 387 3281 2531 Fragments arranged by size: 3281 2531 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 3052 3084 4840 Size: 32 135 32 440 32 1008 32 1756 Cuts at: 4840 4872 4914 4946 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1756 1008 440 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2073 3543 3789 669 Size: 988 281 135 1470 246 3079 Fragments arranged by size: 3079 1470 988 281 246 135 HindIII A'AGCT_T Cuts at: 748 748 Size: 6199 HpaI GTT'AAC Cuts at: 3789 3789 Size: 6199 KpnI G_GTAC'C Cuts at: 3524 3524 Size: 6199 MfeI C'AATT_G Cuts at: 3798 3798 Size: 6199 MluI A'CGCG_T Cuts at: 6075 6075 Size: 6199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2392 5313 5497 1305 Size: 1087 2921 184 2007 Fragments arranged by size: 2921 2007 1087 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4377 Fragments arranged by size: 4377 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2339 4157 4516 4675 518 Size: 648 1173 1818 359 159 2042 Fragments arranged by size: 2042 1818 1173 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3615 4292 5440 5685 1606 Size: 2009 677 1148 245 2120 Fragments arranged by size: 2120 2009 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5647 NdeI CA'TA_TG Cuts at: 387 387 Size: 6199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6199 NotI GC'GGCC_GC Cuts at: 3646 3646 Size: 6199 NspI r_CATG'y Cuts at: 1132 1229 3557 5102 1132 Size: 97 2328 1545 2229 Fragments arranged by size: 2328 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6199 PciI A'CATG_T Cuts at: 5098 5098 Size: 6199 PflMI CCAn_nnn'nTGG Cuts at: 1108 3030 3105 1108 Size: 1922 75 4202 Fragments arranged by size: 4202 1922 75 PmeI GTTT'AAAC Cuts at: 3503 3503 Size: 6199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4268 4300 5781 5813 4268 Size: 32 1481 32 4654 Fragments arranged by size: 4654 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2131 2131 Size: 6199 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6199 PsiI TTA'TAA Cuts at: 3769 3769 Size: 6199 PstI C_TGCA'G Cuts at: 830 3047 3551 830 Size: 2217 504 3478 Fragments arranged by size: 3478 2217 504 PvuI CG_AT'CG Cuts at: 2001 2167 2001 Size: 166 6033 SalI G'TCGA_C Cuts at: 1936 2071 3541 1936 Size: 135 1470 4594 Fragments arranged by size: 4594 1470 135 SanDI GG'GwC_CC Cuts at: 2131 2131 Size: 6199 SbfI CC_TGCA'GG Cuts at: 3551 3551 Size: 6199 ScaI AGT'ACT Cuts at: 4447 6159 4447 Size: 1712 4487 SfcI C'TryA_G Cuts at: 826 1045 2188 3043 3547 4682 5363 5554 826 Size: 219 1143 855 504 1135 681 191 1471 Fragments arranged by size: 1471 1143 1135 855 681 504 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6199 SmaI CCC'GGG Cuts at: 1872 3526 1872 Size: 1654 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4303 5204 5466 820 Size: 197 926 2360 901 262 1553 Fragments arranged by size: 2360 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3990 493 Size: 3497 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6199 SphI G_CATG'C Cuts at: 1132 3557 1132 Size: 2425 3774 SspI AAT'ATT Cuts at: 5 52 2466 2756 4123 5 Size: 47 2414 290 1367 2081 Fragments arranged by size: 2414 2081 1367 290 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6199 StyI C'CwwG_G Cuts at: 513 1065 2956 3638 513 Size: 552 1891 682 3074 Fragments arranged by size: 3074 1891 682 552 SwaI ATTT'AAAT Cuts at: 2652 2652 Size: 6199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2685 4226 4243 4396 4581 5026 325 Size: 1255 1105 1541 17 153 185 445 1498 Fragments arranged by size: 1541 1498 1255 1105 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2215 2938 4445 Size: 274 80 33 51 1443 238 723 1507 Cuts at: 4445 6157 96 Size: 1712 138 Fragments arranged by size: 1712 1507 1443 723 274 238 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3439 3471 6159 6191 3439 Size: 32 2688 32 3447 Fragments arranged by size: 3447 2688 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6199 XbaI T'CTAG_A Cuts at: 3535 3535 Size: 6199 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3106 3106 Size: 6199 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6199 XmaI C'CCGG_G Cuts at: 1870 3524 1870 Size: 1654 4545 XmnI GAAnn'nnTTC Cuts at: 4328 4328 Size: 6199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4007 276 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI