################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BciVI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BseAI BsgI BsiWI BsmAI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MflI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AfaI 204 GTAC GT^AC AlwI 3 GATCC GGATC(4/5) AlwNI 449 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 956 GGGCCC GGGCC^C AscI 328 GGCGCGCC GG^CGCGCC Asp700I 916 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 304 GAGCAC G[AT]GC[AT]^C AvaII 906 GGACC G^G[AT]CC BbsI 650 GTCTTC GAAGAC(2/6) BcgI 80 CGAAGCGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 891 TGATCA T^GATCA BlpI 243 GCTCAGC GC^T[ACGT]AGC BpmI 544 CTCCAG CTGGAG(16/14) Bpu1102I 243 GCTCAGC GC^T[ACGT]AGC BpuAI 650 GTCTTC GAAGAC(2/6) BsaWI 658 ACCGGA [AT]^CCGG[AT] BsiHKAI 304 GAGCAC G[AT]GC[AT]^C BsmI 483 GCATTC GAATGC(1/-1) BsrBI 163 CCGCTC CCGCTC(-3/-3) BsrFI 965 GCCGGC [GA]^CCGG[CT] BsrI 1030 ACTGG ACTGG(1/-1) BssHII 329 GCGCGC G^CGCGC CelII 243 GCTCAGC GC^T[ACGT]AGC Cfr10I 965 GCCGGC [GA]^CCGG[CT] DraIII 232 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 740 CCTACGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 23 CCATGG C^C[AT][AT]GG FbaI 891 TGATCA T^GATCA MseI 466 TTAA T^TAA MslI 565 CATACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 965 GCCGGC GCC^GGC NcoI 23 CCATGG C^CATGG NgoMIV 965 GCCGGC G^CCGGC PflMI 598 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 905 AGGACCC [GA]G^G[AT]CC[CT] RsaI 204 GTAC GT^AC SfiI 255 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 415 CTCAAG C^T[CT][GA]AG StyI 23 CCATGG C^C[AT][AT]GG TaqI 14 TCGA T^CGA Tru9I 466 TTAA T^TAA TspRI 724 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 14 TCGA T^CGA Van91I 598 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 411 CCACCTCAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 916 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 18 GGCGCC G[GA]^CG[CT]C 1035 GGCGCC AvaI 309 CTCGGG C^[CT]CG[GA]G 366 CTCGGG BanI 18 GGCGCC G^G[CT][GA]CC 1035 GGCGCC BbeI 18 GGCGCC GGCGC^C 1035 GGCGCC BsaHI 18 GGCGCC G[GA]^CG[CT]C 1035 GGCGCC BseRI 382 GAGGAG GAGGAG(10/8) 760 GAGGAG BsiEI 275 CGGCCG CG[GA][CT]^CG 812 CGGCCG BsoBI 309 CTCGGG C^[CT]CG[GA]G 366 CTCGGG EagI 275 CGGCCG C^GGCCG 812 CGGCCG EclXI 275 CGGCCG C^GGCCG 812 CGGCCG Eco52I 275 CGGCCG C^GGCCG 812 CGGCCG HgaI 240 GACGC GACGC(5/10) 648 GCGTC Hin1I 18 GGCGCC G[GA]^CG[CT]C 1035 GGCGCC KasI 18 GGCGCC G^GCGCC 1035 GGCGCC KspI 257 CCGCGG CCGC^GG 809 CCGCGG NarI 18 GGCGCC GG^CGCC 1035 GGCGCC PleI 73 GACTC GAGTC(4/5) 561 GACTC PstI 287 CTGCAG CTGCA^G 970 CTGCAG SacII 257 CCGCGG CCGC^GG 809 CCGCGG SfcI 287 CTGCAG C^T[GA][CT]AG 970 CTGCAG SfoI 18 GGCGCC GGC^GCC 1035 GGCGCC Tsp45I 238 GTGAC ^GT[GC]AC 590 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AluI BanII BbvI BcnI BglI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TfiI TseI