(Circular) (Six-base) MAPSORT of: pFN21AB7693.seq Check: 1625 from: 1 to: 5755 pFN21AB7693 5755 bp With 182 enzymes: SgfI * March 28, 2012 12:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2176 2777 2912 3818 5202 5470 1632 Size: 544 601 135 906 1384 268 1917 Fragments arranged by size: 1917 1384 906 601 544 268 135 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AlwNI CAG_nnn'CTG Cuts at: 2459 3498 5070 2459 Size: 1039 1572 3144 Fragments arranged by size: 3144 1572 1039 ApaI G_GGCC'C Cuts at: 2965 2965 Size: 5755 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 3064 3290 3442 5551 1493 Size: 1571 226 152 2109 1697 Fragments arranged by size: 2109 1697 1571 226 152 AscI GG'CGCG_CC Cuts at: 2334 2334 Size: 5755 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 2314 2371 3080 1870 Size: 73 371 57 709 4545 Fragments arranged by size: 4545 709 371 73 57 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2023 3040 3076 4531 618 Size: 325 217 627 236 1017 36 1455 1842 Fragments arranged by size: 1842 1455 1017 627 325 236 217 36 BanII G_rGCy'C Cuts at: 1391 2772 2965 3007 3074 1391 Size: 1381 193 42 67 4072 Fragments arranged by size: 4072 1381 193 67 42 BbeI G_GCGC'C Cuts at: 2027 3044 2027 Size: 1017 4738 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2648 928 Size: 971 749 4035 Fragments arranged by size: 4035 971 749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2074 2108 3946 3980 1903 Size: 34 137 34 1838 34 3678 Fragments arranged by size: 3678 1838 137 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 2896 1548 Size: 1348 4407 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2267 2279 2354 Size: 107 122 71 631 605 595 12 75 Cuts at: 2354 4366 136 Size: 2012 1525 Fragments arranged by size: 2012 1525 631 605 595 122 107 75 71 12 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 2249 3172 2249 Size: 923 4832 Bme1580I G_kGCm'C Cuts at: 1790 2965 3816 4972 1790 Size: 1175 851 1156 2573 Fragments arranged by size: 2573 1175 1156 851 BmgBI CAC'GTC Cuts at: 1318 2438 1318 Size: 1120 4635 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3045 3486 4444 Size: 416 53 83 399 24 1628 441 958 Cuts at: 4444 442 Size: 1753 Fragments arranged by size: 1753 1628 958 441 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2534 4415 1239 Size: 1295 1881 2579 Fragments arranged by size: 2579 1881 1295 Bpu10I CC'TnA_GC Cuts at: 2702 2702 Size: 5755 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2405 3880 4745 5043 2405 Size: 1475 865 298 3117 Fragments arranged by size: 3117 1475 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2024 3041 3562 3944 Size: 53 83 186 936 491 1017 521 382 Cuts at: 3944 4579 275 Size: 635 1451 Fragments arranged by size: 1451 1017 936 635 521 491 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2663 4188 4586 4860 5007 5418 2663 Size: 1525 398 274 147 411 3000 Fragments arranged by size: 3000 1525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5530 Fragments arranged by size: 5530 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2402 2780 1186 Size: 1216 378 4161 Fragments arranged by size: 4161 1216 378 BseYI C'CCAG_C Cuts at: 2220 2964 4958 5454 2220 Size: 744 1994 496 2521 Fragments arranged by size: 2521 1994 744 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2283 2820 3205 3966 4994 1540 Size: 275 186 282 537 385 761 1028 2301 Fragments arranged by size: 2301 1028 761 537 385 282 275 186 BsiHKAI G_wGCw'C Cuts at: 2313 3816 3901 4972 2313 Size: 1503 85 1071 3096 Fragments arranged by size: 3096 1503 1071 85 BsmI GAATG_Cn' Cuts at: 2488 3266 3359 2488 Size: 778 93 4884 Fragments arranged by size: 4884 778 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2313 2772 2965 3007 3074 3816 3901 Size: 399 523 459 193 42 67 742 85 Cuts at: 3901 4972 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 523 459 399 193 85 67 42 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3110 844 Size: 2266 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2170 3642 1394 Size: 429 347 1472 3507 Fragments arranged by size: 3507 1472 429 347 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2970 4399 4586 1700 Size: 110 1160 1429 187 2869 Fragments arranged by size: 2869 1429 1160 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 2334 1925 Size: 409 5346 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstYI r'GATC_y Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750 Size: 1113 367 385 17 1452 86 12 346 Cuts at: 5750 1972 Size: 1977 Fragments arranged by size: 1977 1452 1113 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2028 2213 2262 2534 2772 2814 513 Size: 552 963 185 49 272 238 42 3454 Fragments arranged by size: 3454 963 552 272 238 185 49 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 3271 4065 4085 3271 Size: 794 20 4941 Fragments arranged by size: 4941 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DraIII CAC_nnn'GTG Cuts at: 2242 2242 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2326 2413 Size: 54 1425 50 275 18 450 46 87 Cuts at: 2413 2817 3202 4091 5720 8 Size: 404 385 889 1629 43 Fragments arranged by size: 1629 1425 889 450 404 385 275 87 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2280 2817 3202 1537 Size: 275 468 537 385 4090 Fragments arranged by size: 4090 537 468 385 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2753 2934 2948 3685 1353 Size: 1400 181 14 737 3423 Fragments arranged by size: 3423 1400 737 181 14 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2048 2350 4342 4716 4862 387 Size: 1064 138 459 302 1992 374 146 1280 Fragments arranged by size: 1992 1280 1064 459 374 302 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2534 2777 2912 3818 4415 5202 Size: 393 544 358 243 135 906 597 787 Cuts at: 5202 5470 1239 Size: 268 1524 Fragments arranged by size: 1524 906 787 597 544 393 358 268 243 135 EcoNI CCTnn'n_nnAGG Cuts at: 2749 2749 Size: 5755 EcoO109I rG'GnC_Cy Cuts at: 2583 2911 2961 2583 Size: 328 50 5377 Fragments arranged by size: 5377 328 50 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 2027 2221 2971 2995 3044 4902 1678 Size: 349 194 750 24 49 1858 2531 Fragments arranged by size: 2531 1858 750 349 194 49 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 2572 2604 4396 Size: 32 135 32 905 32 63 32 1792 Cuts at: 4396 4428 4470 4502 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1792 905 135 63 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KasI G'GCGC_C Cuts at: 2023 3040 2023 Size: 1017 4738 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2458 3073 4869 5053 1305 Size: 1153 615 1796 184 2007 Fragments arranged by size: 2007 1796 1153 615 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3933 Fragments arranged by size: 3933 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2574 3713 4072 4231 518 Size: 648 1408 1139 359 159 2042 Fragments arranged by size: 2042 1408 1139 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2264 2412 2816 2986 3171 3848 4996 5241 Size: 658 148 404 170 185 677 1148 245 Cuts at: 5241 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 658 404 245 185 170 148 NaeI GCC'GGC Cuts at: 2972 2972 Size: 5755 NarI GG'CG_CC Cuts at: 2024 3041 2024 Size: 1017 4738 NcoI C'CATG_G Cuts at: 513 1065 2028 513 Size: 552 963 4240 Fragments arranged by size: 4240 963 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NgoMIV G'CCGG_C Cuts at: 2970 2970 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 3113 4658 1132 Size: 97 1884 1545 2229 Fragments arranged by size: 2229 1884 1545 97 PasI CC'CwG_GG Cuts at: 1348 2255 1348 Size: 907 4848 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 2609 1108 Size: 1501 4254 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2911 2911 Size: 5755 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PspOMI G'GGCC_C Cuts at: 2961 2961 Size: 5755 PstI C_TGCA'G Cuts at: 830 2296 2979 3107 830 Size: 1466 683 128 3478 Fragments arranged by size: 3478 1466 683 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 SacII CC_GC'GG Cuts at: 2265 2817 2265 Size: 552 5203 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2292 2975 3103 4238 4919 5110 826 Size: 219 1247 683 128 1135 681 191 1471 Fragments arranged by size: 1471 1247 1135 683 681 219 191 128 SfiI GGCCn_nnn'nGGCC Cuts at: 2267 2267 Size: 5755 SfoI GGC'GCC Cuts at: 2025 3042 2025 Size: 1017 4738 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2420 3859 4760 5022 820 Size: 197 926 477 1439 901 262 1553 Fragments arranged by size: 1553 1439 926 901 477 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2028 3194 513 Size: 552 963 1166 3074 Fragments arranged by size: 3074 1166 963 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2092 2729 3782 3799 3952 4137 4582 Size: 1255 512 637 1053 17 153 185 445 Cuts at: 4582 325 Size: 1498 Fragments arranged by size: 1498 1255 1053 637 512 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5715 5747 5715 Size: 32 5723 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2423 2423 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 2925 3884 2925 Size: 959 4796 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI SwaI