################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I TspRI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AfaI 405 GTAC GT^AC AhdI 352 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 340 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 352 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 37 GTGCTC G[AT]GC[AT]^C BlpI 39 GCTCAGC GC^T[ACGT]AGC BmyI 37 GTGCTC G[AGT]GC[ACT]^C BpmI 355 CTGGAG CTGGAG(16/14) Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC BsaI 304 GAGACC GGTCTC(1/5) BseRI 190 GAGGAG GAGGAG(10/8) BsiHKAI 37 GTGCTC G[AT]GC[AT]^C BsiYI 183 CCCCCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 183 CCCCCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C BspHI 512 TCATGA T^CATGA BsrI 21 ACTGG ACTGG(1/-1) BstYI 517 AGATCC [GA]^GATC[CT] CelII 39 GCTCAGC GC^T[ACGT]AGC DraII 106 AGGGCCT [GA]G^G[ACGT]CC[CT] Eam1105I 352 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 106 AGGGCCT [GA]G^G[ACGT]CC[CT] HphI 384 GGTGA GGTGA(8/7) MaeIII 98 GTAAC ^GT[ACGT]AC MflI 517 AGATCC [GA]^GATC[CT] MspA1I 343 CAGCTG C[AC]G^C[GT]G MwoI 59 GCTCTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PvuII 343 CAGCTG CAG^CTG RcaI 512 TCATGA T^CATGA RsaI 405 GTAC GT^AC SfaNI 369 GATGC GCATC(5/9) SmlI 221 CTCAAG C^T[CT][GA]AG TaqI 329 TCGA T^CGA TthHB8I 329 TCGA T^CGA XbaI 312 TCTAGA T^CTAGA XhoII 517 AGATCC [GA]^GATC[CT] XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 178 GAATTC [GA]^AATT[CT] 319 GAATTC AlwI 249 GATCC GGATC(4/5) 518 GATCC ApoI 178 GAATTC [GA]^AATT[CT] 319 GAATTC BbvI 342 GCAGC GCAGC(8/12) 292 GCTGC BfaI 31 CTAG C^TAG 313 CTAG BsmAI 304 GAGAC GTCTC(1/5) 393 GTCTC BstF5I 417 CATCC GGATG(2/0) 300 GGATG Cfr13I 27 GGCCC G^G[ACGT]CC 107 GGGCC DdeI 40 CTCAG C^T[ACGT]AG 80 CTCAG EarI 268 CTCTTC CTCTTC(1/4) 555 GAAGAG EcoRI 178 GAATTC G^AATTC 319 GAATTC Fnu4HI 292 GCTGC GC^[ACGT]GC 342 GCAGC FokI 417 CATCC GGATG(9/13) 300 GGATG ItaI 292 GCTGC GC^[ACGT]GC 342 GCAGC Ksp632I 268 CTCTTC CTCTTC(1/4) 555 GAAGAG MaeI 31 CTAG C^TAG 313 CTAG Sau96I 27 GGCCC G^G[ACGT]CC 107 GGGCC TfiI 137 GAATC G^A[AT]TC 446 GAATC TseI 292 GCTGC G^C[AT]GC 342 GCAGC Tsp509I 179 AATT ^AATT 320 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI BsaJI BssKI BstNI DpnII EcoRII HaeIII HinfI MboI MboII MnlI MvaI NdeII NlaIII PleI Sau3AI ScrFI