Restriction map for pFN21AE1670
 (Circular) (Six-base) MAPSORT of: pFN21AE1670.seq  Check: 9321  from: 1  to: 5269



pFN21AE1670  5269 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:48  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3079     278 
   Size:         53      83     186     936    1543    2468

  Fragments arranged by size: 

                2468    1543     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2612    1937 
   Size:        675    4594
 
Acc65I G'GTAC_C

Cuts at:   2590    2590 
   Size:       5269
 
AclI AA'CG_TT

Cuts at:   1563    3396    3769    1563 
   Size:       1833     373    3063

  Fragments arranged by size: 

                3063    1833     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2257    3332    4716    4984    1632 
   Size:        625    1075    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1075     625     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5072
 
AflIII A'CryG_T

Cuts at:   1203    4168    5145    1203 
   Size:       2965     977    1327

  Fragments arranged by size: 

                2965    1327     977
 
AgeI A'CCGG_T

Cuts at:   4100    4100 
   Size:       5269
 
AhdI GACnn_n'nnGTC

Cuts at:   3998    3998 
   Size:       5269
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5269
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5269
 
AlwNI CAG_nnn'CTG

Cuts at:   2347    3012    4584    2347 
   Size:        665    1572    3032

  Fragments arranged by size: 

                3032    1572     665
 
ApaLI G'TGCA_C

Cuts at:   3326    4482    3326 
   Size:       1156    4113
 
ApoI r'AATT_y

Cuts at:   1493    2321    2578    2804    2956    5065    1493 
   Size:        828     257     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     828     257     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3823     160 
   Size:        872    2791    1606

  Fragments arranged by size: 

                2791    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5269
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2594    1870 
   Size:         73     651    4545

  Fragments arranged by size: 

                4545     651      73
 
BamHI G'GATC_C

Cuts at:   2599    2966    2599 
   Size:        367    4902
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2590    4045     618 
   Size:        325     217     627     803    1455    1842

  Fragments arranged by size: 

                1842    1455     803     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2588    1391 
   Size:       1197    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4298
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3460    3494    1903 
   Size:         34    1523      34    3678

  Fragments arranged by size: 

                3678    1523      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3161    4371    3161 
   Size:       1210    4059
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5269
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3880     136 
   Size:        107     122      71     631     605    2208    1525

  Fragments arranged by size: 

                2208    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2259    2519    5264    2259 
   Size:        260    2745    2264

  Fragments arranged by size: 

                2745    2264     260
 
BlpI GC'TnA_GC

Cuts at:   2045    2686    2045 
   Size:        641    4628
 
Bme1580I G_kGCm'C

Cuts at:   1790    3330    4486    1790 
   Size:       1540    1156    2573

  Fragments arranged by size: 

                2573    1540    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5269
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3000    3958     442 
   Size:        416      53      83     399      24    1583     958    1753

  Fragments arranged by size: 

                1753    1583     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5269
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2163    2163 
   Size:       5269
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3929    1239 
   Size:       2690    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3394    4259    4557    3394 
   Size:        865     298    4106

  Fragments arranged by size: 

                4106     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2300    3932     882 
   Size:        617     801    1632    2219

  Fragments arranged by size: 

                2219    1632     801     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3060     493 
   Size:        711    1856    2702

  Fragments arranged by size: 

                2702    1856     711
 
BsaBI GATnn'nnATC

Cuts at:   5263    5263 
   Size:       5269
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3076    3458    4093     275 
   Size:         53      83     186     936    1543     382     635    1451

  Fragments arranged by size: 

                1543    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3702    4100    4374    4521    4932    3702 
   Size:        398     274     147     411    4039

  Fragments arranged by size: 

                4039     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2380    2410    1178 
   Size:         30     165      30     977      30    4037

  Fragments arranged by size: 

                4037     977     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2152    2210    1186 
   Size:        966      58    4245

  Fragments arranged by size: 

                4245     966      58
 
BseYI C'CCAG_C

Cuts at:   4472    4968    4472 
   Size:        496    4773
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4696
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2719    3480    4508    1540 
   Size:        275     186     718     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     718     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2046    3330    3415    4486    2046 
   Size:       1284      85    1071    2829

  Fragments arranged by size: 

                2829    1284    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2780    2873    2780 
   Size:         93    5176
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2046    2588    3330    3415    4486    1391 
   Size:        399     256     542     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     542     399     256      85
 
BspEI T'CCGG_A

Cuts at:   4932    4932 
   Size:       5269
 
BspHI T'CATG_A

Cuts at:   2514    3158    4888    2514 
   Size:        644    1730    2895

  Fragments arranged by size: 

                2895    1730     644
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2624     844 
   Size:       1780    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3156    1394 
   Size:        429    1333    3507

  Fragments arranged by size: 

                3507    1333     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3764    3938      65 
   Size:       1183    2516     174    1396

  Fragments arranged by size: 

                2516    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3913    4100    1700 
   Size:        110    2103     187    2869

  Fragments arranged by size: 

                2869    2103     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5269
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5269
 
BssSI C'ACGA_G

Cuts at:   3329    4341    3329 
   Size:       1012    4257
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5269
 
BstBI TT'CG_AA

Cuts at:   4073    4073 
   Size:       5269
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5269
 
BstYI r'GATC_y

Cuts at:   1972    2259    2519    2599    2966    3351    3368    4820    4906 
   Size:        287     260      80     367     385      17    1452      86

Cuts at:   4906    4918    5264    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     287     260      86
                  80      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3778

  Fragments arranged by size: 

                3778     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4530

  Fragments arranged by size: 

                4530     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2785    3579    3599    2785 
   Size:        794      20    4455

  Fragments arranged by size: 

                4455     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5269
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5234
 
DraI TTT'AAA

Cuts at:   2573    2573 
   Size:       5269
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4276     809 
   Size:        507    2960    1802

  Fragments arranged by size: 

                2960    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2716    3605    5234 
   Size:         54    1425      50     275      18     886     889    1629

Cuts at:   5234       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     886     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2716    1537 
   Size:        275     904    4090

  Fragments arranged by size: 

                4090     904     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2276    3199    1353 
   Size:        923     923    3423

  Fragments arranged by size: 

                3423     923     923
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3856    4230    4376     387 
   Size:       1064     138    2267     374     146    1280

  Fragments arranged by size: 

                2267    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2257    3332    3929    4716    4984    1239 
   Size:        393     625    1075     597     787     268    1524

  Fragments arranged by size: 

                1524    1075     787     625     597     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2405    2405 
   Size:       5269
 
EcoO109I rG'GnC_Cy

Cuts at:   2112    2112 
   Size:       5269
 
EcoRI G'AATT_C

Cuts at:   2578    2956    2578 
   Size:        378    4891
 
FspI TGC'GCA

Cuts at:   3775    3775 
   Size:       5269
 
HaeII r_GCGC'y

Cuts at:   1678    4416    1678 
   Size:       2738    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2251    2283    2380    2412    2496 
   Size:         32     135      32     679      32      97      32      84

Cuts at:   2496    2528    3910    3942    3984    4016    1373 
   Size:         32    1382      32      42      32    2626

  Fragments arranged by size: 

                2626    1382     679     135      97      84      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2535    2613    2859     669 
   Size:        988     281     597      78     246    3079

  Fragments arranged by size: 

                3079     988     597     281     246      78
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5269
 
HpaI GTT'AAC

Cuts at:   2859    2859 
   Size:       5269
 
KpnI G_GTAC'C

Cuts at:   2594    2594 
   Size:       5269
 
MfeI C'AATT_G

Cuts at:   2868    2868 
   Size:       5269
 
MluI A'CGCG_T

Cuts at:   5145    5145 
   Size:       5269
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4383    4567    1305 
   Size:       3078     184    2007

  Fragments arranged by size: 

                3078    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3447

  Fragments arranged by size: 

                3447    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3227    3586    3745     518 
   Size:        648    2061     359     159    2042

  Fragments arranged by size: 

                2061    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2049    2347    2685    3362    4510    4755    1606 
   Size:        443     298     338     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     443     338     298     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3778

  Fragments arranged by size: 

                3778     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5269
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5269
 
NotI GC'GGCC_GC

Cuts at:   2716    2716 
   Size:       5269
 
NspI r_CATG'y

Cuts at:   1132    1229    2627    4172    1132 
   Size:         97    1398    1545    2229

  Fragments arranged by size: 

                2229    1545    1398      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5269
 
PciI A'CATG_T

Cuts at:   4168    4168 
   Size:       5269
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2098    1108 
   Size:        990    4279
 
PmeI GTTT'AAAC

Cuts at:   2573    2573 
   Size:       5269
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3338    3370    4851    4883    3338 
   Size:         32    1481      32    3724

  Fragments arranged by size: 

                3724    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2112    2112 
   Size:       5269
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5269
 
PsiI TTA'TAA

Cuts at:   2839    2839 
   Size:       5269
 
PstI C_TGCA'G

Cuts at:    830    2621     830 
   Size:       1791    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5269
 
PvuII CAG'CTG

Cuts at:   2049    2347    2049 
   Size:        298    4971
 
SalI G'TCGA_C

Cuts at:   1936    2611    1936 
   Size:        675    4594
 
SbfI CC_TGCA'GG

Cuts at:   2621    2621 
   Size:       5269
 
ScaI AGT'ACT

Cuts at:   3517    5229    3517 
   Size:       1712    3557
 
SfcI C'TryA_G

Cuts at:    826    1045    2617    3752    4433    4624     826 
   Size:        219    1572    1135     681     191    1471

  Fragments arranged by size: 

                1572    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5269
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5269
 
SmaI CCC'GGG

Cuts at:   1872    2596    1872 
   Size:        724    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3373    4274    4536     820 
   Size:        197     926    1430     901     262    1553

  Fragments arranged by size: 

                1553    1430     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3060     493 
   Size:       2567    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5269
 
SphI G_CATG'C

Cuts at:   1132    2627    1132 
   Size:       1495    3774
 
SspI AAT'ATT

Cuts at:      5      52    3193       5 
   Size:         47    3141    2081

  Fragments arranged by size: 

                3141    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5269
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2708     513 
   Size:        552     939     704    3074

  Fragments arranged by size: 

                3074     939     704     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3296    3313    3466    3651    4096     325 
   Size:       1255    1716      17     153     185     445    1498

  Fragments arranged by size: 

                1716    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3515    5227      96 
   Size:        274      80      33      51    1443    1538    1712     138

  Fragments arranged by size: 

                1712    1538    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5229    5261    5229 
   Size:         32    5237
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5269
 
XbaI T'CTAG_A

Cuts at:   2605    2605 
   Size:       5269
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2198    2198 
   Size:       5269
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5269
 
XmaI C'CCGG_G

Cuts at:   1870    2594    1870 
   Size:        724    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2438    3398    2438 
   Size:        960    4309
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3077     276 
   Size:         53      83     186     936    1543    2468

  Fragments arranged by size: 

                2468    1543     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI
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