################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp509I TspRI Tth111I TthHB8I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AfaI 474 GTAC GT^AC AlwI 518 GATCC GGATC(4/5) AlwNI 340 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 37 GTGCTC G[AT]GC[AT]^C AvaII 107 GGACC G^G[AT]CC BfaI 313 CTAG C^TAG BlpI 39 GCTCAGC GC^T[ACGT]AGC BmyI 37 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC BsiHKAI 37 GTGCTC G[AT]GC[AT]^C BsmFI 391 GGGAC GGGAC(10/14) Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C BspHI 512 TCATGA T^CATGA BsrI 207 CCAGT ACTGG(1/-1) BstYI 517 AGATCC [GA]^GATC[CT] CelII 39 GCTCAGC GC^T[ACGT]AGC EcoNI 237 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeIII 27 GGCC GG^CC HphI 246 GGTGA GGTGA(8/7) MaeI 313 CTAG C^TAG MaeIII 98 GTCAC ^GT[ACGT]AC MflI 517 AGATCC [GA]^GATC[CT] MseI 553 TTAA T^TAA MwoI 47 GCAAGTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 512 TCATGA T^CATGA RsaI 474 GTAC GT^AC Tru9I 553 TTAA T^TAA Tsp45I 98 GTCAC ^GT[GC]AC Van91I 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 312 TCTAGA T^CTAGA XcmI 110 CCATGATGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 517 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BseRI 132 GAGGAG GAGGAG(10/8) 190 GAGGAG BsiYI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 237 CCTCCATGAGG BslI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 237 CCTCCATGAGG BstF5I 417 CATCC GGATG(2/0) 300 GGATG Cfr13I 27 GGCCC G^G[ACGT]CC 107 GGACC DdeI 40 CTCAG C^T[ACGT]AG 80 CTCAG DpnII 73 GATC ^GATC 518 GATC EarI 268 CTCTTC CTCTTC(1/4) 525 CTCTTC FokI 417 CATCC GGATG(9/13) 300 GGATG Ksp632I 268 CTCTTC CTCTTC(1/4) 525 CTCTTC MboI 73 GATC ^GATC 518 GATC MboII 269 TCTTC GAAGA(8/7) 526 TCTTC MspA1I 42 CAGCTG C[AC]G^C[GT]G 343 CAGCTG NdeII 73 GATC ^GATC 518 GATC PvuII 42 CAGCTG CAG^CTG 343 CAGCTG Sau3AI 73 GATC ^GATC 518 GATC Sau96I 27 GGCCC G^G[ACGT]CC 107 GGACC SfaNI 114 GATGC GCATC(5/9) 369 GATGC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AluI BbvI BsaJI BssKI BstNI EcoRII Fnu4HI HinfI ItaI MnlI MvaI NlaIII PleI ScrFI TfiI TseI