################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AlwI 518 GATCC GGATC(4/5) Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 37 GTGCTC G[AT]GC[AT]^C BfaI 313 CTAG C^TAG BlpI 39 GCTCAGC GC^T[ACGT]AGC BmyI 37 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC BsaI 304 GAGACC GGTCTC(1/5) BseRI 132 GAGGAG GAGGAG(10/8) BsiHKAI 37 GTGCTC G[AT]GC[AT]^C Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C BspHI 512 TCATGA T^CATGA BsrDI 5 CATTGC GCAATG(2/0) BsrI 207 CCAGT ACTGG(1/-1) BstYI 517 AGATCC [GA]^GATC[CT] CelII 39 GCTCAGC GC^T[ACGT]AGC Cfr13I 27 GGCCC G^G[ACGT]CC DdeI 40 CTCAG C^T[ACGT]AG DpnII 518 GATC ^GATC HaeIII 27 GGCC GG^CC MaeI 313 CTAG C^TAG MboI 518 GATC ^GATC MflI 517 AGATCC [GA]^GATC[CT] MspA1I 42 CAGCTG C[AC]G^C[GT]G NdeII 518 GATC ^GATC PvuII 42 CAGCTG CAG^CTG RcaI 512 TCATGA T^CATGA Sau3AI 518 GATC ^GATC Sau96I 27 GGCCC G^G[ACGT]CC SmlI 221 CTCAAG C^T[CT][GA]AG XhoII 517 AGATCC [GA]^GATC[CT] XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BstF5I 417 CATCC GGATG(2/0) 300 GGATG EarI 268 CTCTTC CTCTTC(1/4) 388 GAAGAG FokI 417 CATCC GGATG(9/13) 300 GGATG Ksp632I 268 CTCTTC CTCTTC(1/4) 388 GAAGAG MseI 19 TTAA T^TAA 553 TTAA MwoI 47 GCAAGTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 59 GCTCTCTGGGC PleI 393 GACTC GAGTC(4/5) 412 GACTC SfaNI 114 GATGC GCATC(5/9) 249 GATGC TfiI 137 GAATC G^A[AT]TC 446 GAATC Tru9I 19 TTAA T^TAA 553 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AcsI AluI ApoI BbvI BsaJI BsmAI BssKI BstNI EcoRII Fnu4HI HinfI ItaI MboII MnlI MvaI NlaIII ScrFI TseI Tsp509I