################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 319 AAATTT [GA]^AATT[CT] AluI 43 AGCT AG^CT AlwI 249 GATCC GGATC(4/5) AlwNI 340 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 319 AAATTT [GA]^AATT[CT] Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 37 GTGCTC G[AT]GC[AT]^C BbsI 216 GAAGAC GAAGAC(2/6) BbvI 292 GCTGC GCAGC(8/12) BglII 517 AGATCT A^GATCT BlpI 39 GCTCAGC GC^T[ACGT]AGC BmyI 37 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC BpuAI 216 GAAGAC GAAGAC(2/6) BseRI 190 GAGGAG GAGGAG(10/8) BsiHKAI 37 GTGCTC G[AT]GC[AT]^C BsiYI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 391 GAGAC GTCTC(1/5) Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C BspHI 512 TCATGA T^CATGA BsrI 207 CCAGT ACTGG(1/-1) BstYI 517 AGATCT [GA]^GATC[CT] CelII 39 GCTCAGC GC^T[ACGT]AGC DraII 106 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 26 TGGCCG [CT]^GGCC[GA] EcoO109I 106 AGGGCCT [GA]G^G[ACGT]CC[CT] Fnu4HI 292 GCTGC GC^[ACGT]GC ItaI 292 GCTGC GC^[ACGT]GC MflI 517 AGATCT [GA]^GATC[CT] MseI 553 TTAA T^TAA NspI 361 GCATGT [GA]CATG^[CT] PflMI 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 512 TCATGA T^CATGA SmlI 221 CTCAAG C^T[CT][GA]AG SspI 549 AATATT AAT^ATT Tru9I 553 TTAA T^TAA TseI 292 GCTGC G^C[AT]GC Tsp509I 320 AATT ^AATT TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 517 AGATCT [GA]^GATC[CT] XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BfaI 64 CTAG C^TAG 313 CTAG BstF5I 417 CATCC GGATG(2/0) 383 GGATG Cfr13I 3 GGCCC G^G[ACGT]CC 107 GGGCC DdeI 40 CTCAG C^T[ACGT]AG 80 CTCAG EarI 268 CTCTTC CTCTTC(1/4) 388 GAAGAG FokI 417 CATCC GGATG(9/13) 383 GGATG Ksp632I 268 CTCTTC CTCTTC(1/4) 388 GAAGAG MaeI 64 CTAG C^TAG 313 CTAG Sau96I 3 GGCCC G^G[ACGT]CC 107 GGGCC TfiI 137 GAATC G^A[AT]TC 446 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### BsaJI BssKI BstNI DpnII EcoRII HaeIII HinfI MboI MboII MnlI MvaI NdeII NlaIII PleI Sau3AI ScrFI