Restriction map for pFN21AE3050
 (Circular) (Six-base) MAPSORT of: pFN21AE3050.seq  Check: 8376  from: 1  to: 5272



pFN21AE3050  5272 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 12:59  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3082     278 
   Size:         53      83     186     936    1546    2468

  Fragments arranged by size: 

                2468    1546     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2615    1937 
   Size:        678    4594
 
Acc65I G'GTAC_C

Cuts at:   2593    2593 
   Size:       5272
 
AclI AA'CG_TT

Cuts at:   1563    3399    3772    1563 
   Size:       1836     373    3063

  Fragments arranged by size: 

                3063    1836     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2183    2260    3335    4719    4987    1632 
   Size:        551      77    1075    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1075     551     268      77
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5075
 
AflIII A'CryG_T

Cuts at:   1203    4171    5148    1203 
   Size:       2968     977    1327

  Fragments arranged by size: 

                2968    1327     977
 
AgeI A'CCGG_T

Cuts at:   4103    4103 
   Size:       5272
 
AhdI GACnn_n'nnGTC

Cuts at:   4001    4001 
   Size:       5272
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5272
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5272
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2298    2330    2298 
   Size:         32    5240
 
AlwNI CAG_nnn'CTG

Cuts at:   2350    3015    4587    2350 
   Size:        665    1572    3035

  Fragments arranged by size: 

                3035    1572     665
 
ApaLI G'TGCA_C

Cuts at:   3329    4485    3329 
   Size:       1156    4116
 
ApoI r'AATT_y

Cuts at:   1493    2324    2581    2807    2959    5068    1493 
   Size:        831     257     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     831     257     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3826     160 
   Size:        872    2794    1606

  Fragments arranged by size: 

                2794    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5272
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2597    1870 
   Size:         73     654    4545

  Fragments arranged by size: 

                4545     654      73
 
BamHI G'GATC_C

Cuts at:   2602    2969    2602 
   Size:        367    4905
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2593    4048     618 
   Size:        325     217     627     806    1455    1842

  Fragments arranged by size: 

                1842    1455     806     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2591    1391 
   Size:       1200    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2228     928 
   Size:        971     329    3972

  Fragments arranged by size: 

                3972     971     329
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3463    3497    1903 
   Size:         34    1526      34    3678

  Fragments arranged by size: 

                3678    1526      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3164    4374    3164 
   Size:       1210    4062
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5272
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3883     136 
   Size:        107     122      71     631     605    2211    1525

  Fragments arranged by size: 

                2211    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2522    5267    2522 
   Size:       2745    2527
 
BlpI GC'TnA_GC

Cuts at:   2045    2689    2045 
   Size:        644    4628
 
Bme1580I G_kGCm'C

Cuts at:   1790    3333    4489    1790 
   Size:       1543    1156    2573

  Fragments arranged by size: 

                2573    1543    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5272
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3003    3961     442 
   Size:        416      53      83     399      24    1586     958    1753

  Fragments arranged by size: 

                1753    1586     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5272
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3932    1239 
   Size:       2693    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2211    3397    4262    4560    2211 
   Size:       1186     865     298    2923

  Fragments arranged by size: 

                2923    1186     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3935     882 
   Size:        617    2436    2219

  Fragments arranged by size: 

                2436    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3063     493 
   Size:        711    1859    2702

  Fragments arranged by size: 

                2702    1859     711
 
BsaBI GATnn'nnATC

Cuts at:   5266    5266 
   Size:       5272
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3079    3461    4096     275 
   Size:         53      83     186     936    1546     382     635    1451

  Fragments arranged by size: 

                1546    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3705    4103    4377    4524    4935    3705 
   Size:        398     274     147     411    4042

  Fragments arranged by size: 

                4042     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2298    2328    1178 
   Size:         30     165      30     895      30    4122

  Fragments arranged by size: 

                4122     895     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2210    1186 
   Size:       1024    4248
 
BseYI C'CCAG_C

Cuts at:   4475    4971    4475 
   Size:        496    4776
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4699
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2722    3483    4511    1540 
   Size:        275     186     721     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     721     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2046    3333    3418    4489    2046 
   Size:       1287      85    1071    2829

  Fragments arranged by size: 

                2829    1287    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2783    2876    2783 
   Size:         93    5179
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2046    2591    3333    3418    4489    1391 
   Size:        399     256     545     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     545     399     256      85
 
BspEI T'CCGG_A

Cuts at:   4935    4935 
   Size:       5272
 
BspHI T'CATG_A

Cuts at:   2517    3161    4891    2517 
   Size:        644    1730    2898

  Fragments arranged by size: 

                2898    1730     644
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2627     844 
   Size:       1783    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3159    1394 
   Size:        429    1336    3507

  Fragments arranged by size: 

                3507    1336     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3767    3941      65 
   Size:       1183    2519     174    1396

  Fragments arranged by size: 

                2519    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3916    4103    1700 
   Size:        110    2106     187    2869

  Fragments arranged by size: 

                2869    2106     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5272
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5272
 
BssSI C'ACGA_G

Cuts at:   3332    4344    3332 
   Size:       1012    4260
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5272
 
BstBI TT'CG_AA

Cuts at:   4076    4076 
   Size:       5272
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5272
 
BstYI r'GATC_y

Cuts at:   1972    2522    2602    2969    3354    3371    4823    4909    4921 
   Size:        550      80     367     385      17    1452      86      12

Cuts at:   4921    5267    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     550     385     367     346      86      80
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3781

  Fragments arranged by size: 

                3781     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4533

  Fragments arranged by size: 

                4533     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2788    3582    3602    2788 
   Size:        794      20    4458

  Fragments arranged by size: 

                4458     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5272
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5237
 
DraI TTT'AAA

Cuts at:   2576    2576 
   Size:       5272
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4279     809 
   Size:        507    2963    1802

  Fragments arranged by size: 

                2963    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2031    2719    3608 
   Size:         54    1425      50     275      18     201     688     889

Cuts at:   3608    5237       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     688     275     201      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2719    1537 
   Size:        275     907    4090

  Fragments arranged by size: 

                4090     907     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2279    2388    3202    1353 
   Size:        926     109     814    3423

  Fragments arranged by size: 

                3423     926     814     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3859    4233    4379     387 
   Size:       1064     138    2270     374     146    1280

  Fragments arranged by size: 

                2270    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2183    2260    3335    3932    4719    4987    1239 
   Size:        393     551      77    1075     597     787     268    1524

  Fragments arranged by size: 

                1524    1075     787     597     551     393     268      77
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2112    2112 
   Size:       5272
 
EcoRI G'AATT_C

Cuts at:   2581    2959    2581 
   Size:        378    4894
 
FspI TGC'GCA

Cuts at:   3778    3778 
   Size:       5272
 
HaeII r_GCGC'y

Cuts at:   1678    4419    1678 
   Size:       2741    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2254    2276    2286    2308    2499 
   Size:         32     135      32     682      22      10      22     191

Cuts at:   2499    2531    3913    3945    3987    4019    1373 
   Size:         32    1382      32      42      32    2626

  Fragments arranged by size: 

                2626    1382     682     191     135      42      32      32
                  32      32      32      22      22      10
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2616    2862     669 
   Size:        988     281     678     246    3079

  Fragments arranged by size: 

                3079     988     678     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5272
 
HpaI GTT'AAC

Cuts at:   2862    2862 
   Size:       5272
 
KpnI G_GTAC'C

Cuts at:   2597    2597 
   Size:       5272
 
MfeI C'AATT_G

Cuts at:   2871    2871 
   Size:       5272
 
MluI A'CGCG_T

Cuts at:   5148    5148 
   Size:       5272
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4386    4570    1305 
   Size:       3081     184    2007

  Fragments arranged by size: 

                3081    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3450

  Fragments arranged by size: 

                3450    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3230    3589    3748     518 
   Size:        648    2064     359     159    2042

  Fragments arranged by size: 

                2064    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2688    3365    4513    4758    1606 
   Size:       1082     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1082     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3781

  Fragments arranged by size: 

                3781     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5272
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5272
 
NotI GC'GGCC_GC

Cuts at:   2719    2719 
   Size:       5272
 
NspI r_CATG'y

Cuts at:   1132    1229    2370    2630    4175    1132 
   Size:         97    1141     260    1545    2229

  Fragments arranged by size: 

                2229    1545    1141     260      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5272
 
PciI A'CATG_T

Cuts at:   4171    4171 
   Size:       5272
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2098    1108 
   Size:        990    4282
 
PmeI GTTT'AAAC

Cuts at:   2576    2576 
   Size:       5272
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2298    2330    3341    3373    4854    4886    2298 
   Size:         32    1011      32    1481      32    2684

  Fragments arranged by size: 

                2684    1481    1011      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5272
 
PsiI TTA'TAA

Cuts at:   2842    2842 
   Size:       5272
 
PstI C_TGCA'G

Cuts at:    830    2624     830 
   Size:       1794    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5272
 
SalI G'TCGA_C

Cuts at:   1936    2614    1936 
   Size:        678    4594
 
SbfI CC_TGCA'GG

Cuts at:   2624    2624 
   Size:       5272
 
ScaI AGT'ACT

Cuts at:   3520    5232    3520 
   Size:       1712    3560
 
SfcI C'TryA_G

Cuts at:    826    1045    2620    3755    4436    4627     826 
   Size:        219    1575    1135     681     191    1471

  Fragments arranged by size: 

                1575    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5272
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5272
 
SmaI CCC'GGG

Cuts at:   1872    2599    1872 
   Size:        727    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2226    3376    4277    4539     820 
   Size:        197     926     283    1150     901     262    1553

  Fragments arranged by size: 

                1553    1150     926     901     283     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3063     493 
   Size:       2570    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5272
 
SphI G_CATG'C

Cuts at:   1132    2630    1132 
   Size:       1498    3774
 
SspI AAT'ATT

Cuts at:      5      52    2556    3196       5 
   Size:         47    2504     640    2081

  Fragments arranged by size: 

                2504    2081     640      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5272
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2711     513 
   Size:        552     939     707    3074

  Fragments arranged by size: 

                3074     939     707     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3299    3316    3469    3654    4099     325 
   Size:       1255    1719      17     153     185     445    1498

  Fragments arranged by size: 

                1719    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3518    5230      96 
   Size:        274      80      33      51    1443    1541    1712     138

  Fragments arranged by size: 

                1712    1541    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5232    5264    5232 
   Size:         32    5240
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5272
 
XbaI T'CTAG_A

Cuts at:   2608    2608 
   Size:       5272
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5272
 
XmaI C'CCGG_G

Cuts at:   1870    2597    1870 
   Size:        727    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2441    3401    2441 
   Size:        960    4312
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3080     276 
   Size:         53      83     186     936    1546    2468

  Fragments arranged by size: 

                2468    1546     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI     PstI     PvuI     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI
    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com