################################## # NO CUT ENZYMES ( 190 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AlwNI 340 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 37 GTGCTC G[AT]GC[AT]^C BbvI 342 GCAGC GCAGC(8/12) BfaI 313 CTAG C^TAG BlpI 39 GCTCAGC GC^T[ACGT]AGC BmyI 37 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC BseRI 190 GAGGAG GAGGAG(10/8) BsiHKAI 37 GTGCTC G[AT]GC[AT]^C BsmAI 391 GAGAC GTCTC(1/5) Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C BspHI 512 TCATGA T^CATGA BsrI 207 CCAGT ACTGG(1/-1) BstF5I 417 CATCC GGATG(2/0) BstYI 517 AGATCC [GA]^GATC[CT] Cac8I 361 GCCTGC GC[ACGT]^[ACGT]GC CelII 39 GCTCAGC GC^T[ACGT]AGC DraII 106 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 26 TGGCCG [CT]^GGCC[GA] EcoO109I 106 AGGGCCT [GA]G^G[ACGT]CC[CT] Fnu4HI 342 GCAGC GC^[ACGT]GC FokI 417 CATCC GGATG(9/13) ItaI 342 GCAGC GC^[ACGT]GC MaeI 313 CTAG C^TAG MflI 517 AGATCC [GA]^GATC[CT] MspA1I 343 CAGCTG C[AC]G^C[GT]G PflMI 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 343 CAGCTG CAG^CTG RcaI 512 TCATGA T^CATGA SmlI 221 CTCAAG C^T[CT][GA]AG TseI 342 GCAGC G^C[AT]GC TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 53 CCATCTGTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 517 AGATCC [GA]^GATC[CT] XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcsI 319 AAATTT [GA]^AATT[CT] 536 AAATTT AluI 43 AGCT AG^CT 344 AGCT AlwI 249 GATCC GGATC(4/5) 518 GATCC ApoI 319 AAATTT [GA]^AATT[CT] 536 AAATTT BsiYI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 183 CCCCCAGGAGG BslI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 183 CCCCCAGGAGG Cfr13I 3 GGCCC G^G[ACGT]CC 107 GGGCC DdeI 40 CTCAG C^T[ACGT]AG 80 CTCAG EarI 268 CTCTTC CTCTTC(1/4) 388 GAAGAG Ksp632I 268 CTCTTC CTCTTC(1/4) 388 GAAGAG MseI 335 TTAA T^TAA 553 TTAA Sau96I 3 GGCCC G^G[ACGT]CC 107 GGGCC Tru9I 335 TTAA T^TAA 553 TTAA Tsp509I 320 AATT ^AATT 537 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### BsaJI BssKI BstNI DpnII EcoRII HaeIII HinfI MboI MboII MnlI MvaI NdeII NlaIII PleI Sau3AI ScrFI TfiI