(Circular) (Six-base) MAPSORT of: pFN21AE5329.seq Check: 1107 from: 1 to: 5524 pFN21AE5329 5524 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2438 2438 Size: 5524 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3334 278 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2867 1937 Size: 930 4594 Acc65I G'GTAC_C Cuts at: 2845 2845 Size: 5524 AclI AA'CG_TT Cuts at: 1563 3651 4024 1563 Size: 2088 373 3063 Fragments arranged by size: 3063 2088 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2535 2557 3587 4971 5239 1632 Size: 903 22 1030 1384 268 1917 Fragments arranged by size: 1917 1384 1030 903 268 22 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5327 AflIII A'CryG_T Cuts at: 1203 4423 5400 1203 Size: 3220 977 1327 Fragments arranged by size: 3220 1327 977 AgeI A'CCGG_T Cuts at: 4355 4355 Size: 5524 AhdI GACnn_n'nnGTC Cuts at: 4253 4253 Size: 5524 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5524 AlwNI CAG_nnn'CTG Cuts at: 3267 4839 3267 Size: 1572 3952 ApaLI G'TGCA_C Cuts at: 3581 4737 3581 Size: 1156 4368 ApoI r'AATT_y Cuts at: 1493 2833 3059 3211 5320 1493 Size: 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 226 152 AseI AT'TA_AT Cuts at: 160 1032 4078 160 Size: 872 3046 1606 Fragments arranged by size: 3046 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 AvaI C'yCGr_G Cuts at: 1870 1943 2849 1870 Size: 73 906 4545 Fragments arranged by size: 4545 906 73 AvrII C'CTAG_G Cuts at: 2248 2248 Size: 5524 BamHI G'GATC_C Cuts at: 2854 3221 2854 Size: 367 5157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2845 4300 618 Size: 325 217 627 1058 1455 1842 Fragments arranged by size: 1842 1455 1058 627 325 217 BanII G_rGCy'C Cuts at: 1391 2427 2843 1391 Size: 1036 416 4072 Fragments arranged by size: 4072 1036 416 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4553 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3715 3749 1903 Size: 34 1778 34 3678 Fragments arranged by size: 3678 1778 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2473 3416 4626 2473 Size: 943 1210 3371 Fragments arranged by size: 3371 1210 943 BclI T'GATC_A Cuts at: 1548 1548 Size: 5524 BfrBI ATG'CAT Cuts at: 2310 2310 Size: 5524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4135 136 Size: 107 122 71 631 605 2463 1525 Fragments arranged by size: 2463 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5519 5519 Size: 5524 BlpI GC'TnA_GC Cuts at: 2941 2941 Size: 5524 Bme1580I G_kGCm'C Cuts at: 1790 3585 4741 1790 Size: 1795 1156 2573 Fragments arranged by size: 2573 1795 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3255 4213 442 Size: 416 53 83 399 24 1838 958 1753 Fragments arranged by size: 1838 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2488 4184 1239 Size: 1013 236 1696 2579 Fragments arranged by size: 2579 1696 1013 236 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2259 3649 4514 4812 2259 Size: 1390 865 298 2971 Fragments arranged by size: 2971 1390 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4187 882 Size: 617 2688 2219 Fragments arranged by size: 2688 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2599 3315 493 Size: 711 1395 716 2702 Fragments arranged by size: 2702 1395 716 711 BsaBI GATnn'nnATC Cuts at: 5518 5518 Size: 5524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3331 3713 4348 275 Size: 53 83 186 936 1798 382 635 1451 Fragments arranged by size: 1798 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3957 4355 4629 4776 5187 3957 Size: 398 274 147 411 4294 Fragments arranged by size: 4294 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5299 Fragments arranged by size: 5299 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 1186 Size: 1063 4461 BseYI C'CCAG_C Cuts at: 4727 5223 4727 Size: 496 5028 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4951 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2974 3735 4763 1540 Size: 275 186 973 761 1028 2301 Fragments arranged by size: 2301 1028 973 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2427 3585 3670 4741 2427 Size: 1158 85 1071 3210 Fragments arranged by size: 3210 1158 1071 85 BsmI GAATG_Cn' Cuts at: 2132 3035 3128 2132 Size: 903 93 4528 Fragments arranged by size: 4528 903 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2427 2843 3585 3670 4741 1391 Size: 399 637 416 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 637 416 399 85 BspEI T'CCGG_A Cuts at: 5187 5187 Size: 5524 BspHI T'CATG_A Cuts at: 3413 5143 3413 Size: 1730 3794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2438 2879 844 Size: 1594 441 3489 Fragments arranged by size: 3489 1594 441 BsrBI CCG'CTC Cuts at: 1394 1823 3411 1394 Size: 429 1588 3507 Fragments arranged by size: 3507 1588 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2119 2697 4019 4193 65 Size: 1183 871 578 1322 174 1396 Fragments arranged by size: 1396 1322 1183 871 578 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4168 4355 1700 Size: 110 2358 187 2869 Fragments arranged by size: 2869 2358 187 110 BsrGI T'GTAC_A Cuts at: 96 2091 96 Size: 1995 3529 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5524 BssSI C'ACGA_G Cuts at: 3584 4596 3584 Size: 1012 4512 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5524 BstBI TT'CG_AA Cuts at: 4328 4328 Size: 5524 BstEII G'GTnAC_C Cuts at: 1184 2530 1184 Size: 1346 4178 BstXI CCAn_nnnn'nTGG Cuts at: 2230 2230 Size: 5524 BstYI r'GATC_y Cuts at: 1972 2854 3221 3606 3623 5075 5161 5173 5519 Size: 882 367 385 17 1452 86 12 346 Cuts at: 5519 1972 Size: 1977 Fragments arranged by size: 1977 1452 882 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2082 2082 Size: 5524 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4972 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4785 Fragments arranged by size: 4785 683 56 BtsI GCAGTG_nn' Cuts at: 3040 3834 3854 3040 Size: 794 20 4710 Fragments arranged by size: 4710 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5489 DraI TTT'AAA Cuts at: 2828 2828 Size: 5524 DraIII CAC_nnn'GTG Cuts at: 2213 2213 Size: 5524 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4531 809 Size: 507 3215 1802 Fragments arranged by size: 3215 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2468 2616 2971 Size: 54 1425 50 275 18 638 148 355 Cuts at: 2971 3860 5489 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 638 355 275 148 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2971 1537 Size: 275 1159 4090 Fragments arranged by size: 4090 1159 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3454 1353 Size: 2101 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4111 4485 4631 387 Size: 1064 138 2522 374 146 1280 Fragments arranged by size: 2522 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2425 2425 Size: 5524 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2488 2535 2557 3587 4184 4971 Size: 393 620 236 47 22 1030 597 787 Cuts at: 4971 5239 1239 Size: 268 1524 Fragments arranged by size: 1524 1030 787 620 597 393 268 236 47 22 EcoNI CCTnn'n_nnAGG Cuts at: 2252 2525 2252 Size: 273 5251 EcoO109I rG'GnC_Cy Cuts at: 2508 2652 2508 Size: 144 5380 EcoRI G'AATT_C Cuts at: 2833 3211 2833 Size: 378 5146 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2241 2241 Size: 5524 FspI TGC'GCA Cuts at: 4030 4030 Size: 5524 HaeII r_GCGC'y Cuts at: 1678 4671 1678 Size: 2993 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373 Size: 32 135 32 2593 32 42 32 2626 Fragments arranged by size: 2626 2593 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2868 3114 669 Size: 988 281 930 246 3079 Fragments arranged by size: 3079 988 930 281 246 HindIII A'AGCT_T Cuts at: 748 2364 748 Size: 1616 3908 HpaI GTT'AAC Cuts at: 3114 3114 Size: 5524 KpnI G_GTAC'C Cuts at: 2849 2849 Size: 5524 MfeI C'AATT_G Cuts at: 3123 3123 Size: 5524 MluI A'CGCG_T Cuts at: 5400 5400 Size: 5524 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2684 4638 4822 1305 Size: 1379 1954 184 2007 Fragments arranged by size: 2007 1954 1379 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2470 2618 10 Size: 54 1425 343 638 148 2916 Fragments arranged by size: 2916 1425 638 343 148 54 MslI CAynn'nnrTG Cuts at: 518 1166 3482 3841 4000 518 Size: 648 2316 359 159 2042 Fragments arranged by size: 2316 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2242 2577 2940 3617 4765 5010 1606 Size: 636 335 363 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 636 363 335 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4972 NdeI CA'TA_TG Cuts at: 387 387 Size: 5524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5524 NotI GC'GGCC_GC Cuts at: 2971 2971 Size: 5524 NsiI A_TGCA'T Cuts at: 2312 2312 Size: 5524 NspI r_CATG'y Cuts at: 1132 1229 2882 4427 1132 Size: 97 1653 1545 2229 Fragments arranged by size: 2229 1653 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5524 PciI A'CATG_T Cuts at: 4423 4423 Size: 5524 PflMI CCAn_nnn'nTGG Cuts at: 1108 2561 1108 Size: 1453 4071 PfoI T'CCnGG_A Cuts at: 2504 2504 Size: 5524 PmeI GTTT'AAAC Cuts at: 2828 2828 Size: 5524 PmlI CAC'GTG Cuts at: 2599 2599 Size: 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3625 5106 5138 3593 Size: 32 1481 32 3979 Fragments arranged by size: 3979 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2508 2652 2508 Size: 144 5380 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5524 PsiI TTA'TAA Cuts at: 3094 3094 Size: 5524 PstI C_TGCA'G Cuts at: 830 2876 830 Size: 2046 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5524 PvuII CAG'CTG Cuts at: 2242 2577 2242 Size: 335 5189 SacI G_AGCT'C Cuts at: 2427 2427 Size: 5524 SalI G'TCGA_C Cuts at: 1936 2866 1936 Size: 930 4594 SbfI CC_TGCA'GG Cuts at: 2876 2876 Size: 5524 ScaI AGT'ACT Cuts at: 3772 5484 3772 Size: 1712 3812 SexAI A'CCwGG_T Cuts at: 2526 2526 Size: 5524 SfcI C'TryA_G Cuts at: 826 1045 2371 2439 2872 4007 4688 4879 826 Size: 219 1326 68 433 1135 681 191 1471 Fragments arranged by size: 1471 1326 1135 681 433 219 191 68 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5524 SmaI CCC'GGG Cuts at: 1872 2851 1872 Size: 979 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2274 3628 4529 4791 820 Size: 197 926 331 1354 901 262 1553 Fragments arranged by size: 1553 1354 926 901 331 262 197 SnaBI TAC'GTA Cuts at: 493 3315 493 Size: 2822 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5524 SphI G_CATG'C Cuts at: 1132 2882 1132 Size: 1750 3774 SspI AAT'ATT Cuts at: 5 52 3448 5 Size: 47 3396 2081 Fragments arranged by size: 3396 2081 47 StuI AGG'CCT Cuts at: 1517 2258 2520 1517 Size: 741 262 4521 Fragments arranged by size: 4521 741 262 StyI C'CwwG_G Cuts at: 513 1065 2158 2248 2963 513 Size: 552 1093 90 715 3074 Fragments arranged by size: 3074 1093 715 552 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3551 3568 3721 3906 4351 325 Size: 1255 1971 17 153 185 445 1498 Fragments arranged by size: 1971 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2091 3770 5482 Size: 274 80 33 51 1443 114 1679 1712 Cuts at: 5482 96 Size: 138 Fragments arranged by size: 1712 1679 1443 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2136 2168 5484 5516 2136 Size: 32 3316 32 2144 Fragments arranged by size: 3316 2144 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5524 XbaI T'CTAG_A Cuts at: 2860 2860 Size: 5524 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5524 XmaI C'CCGG_G Cuts at: 1870 2849 1870 Size: 979 4545 XmnI GAAnn'nnTTC Cuts at: 3653 3653 Size: 5524 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3332 276 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI