(Circular) (Six-base) MAPSORT of: pFN21AE5332.seq Check: 4514 from: 1 to: 5575 pFN21AE5332 5575 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3385 278 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2918 1937 Size: 981 4594 Acc65I G'GTAC_C Cuts at: 2896 2896 Size: 5575 AclI AA'CG_TT Cuts at: 1563 3702 4075 1563 Size: 2139 373 3063 Fragments arranged by size: 3063 2139 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2246 2285 3638 5022 5290 1632 Size: 614 39 1353 1384 268 1917 Fragments arranged by size: 1917 1384 1353 614 268 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5378 AflIII A'CryG_T Cuts at: 1203 2465 4474 5451 1203 Size: 1262 2009 977 1327 Fragments arranged by size: 2009 1327 1262 977 AgeI A'CCGG_T Cuts at: 4406 4406 Size: 5575 AhdI GACnn_n'nnGTC Cuts at: 4304 4304 Size: 5575 AleI CACnn'nnGTG Cuts at: 1166 2312 1166 Size: 1146 4429 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5575 AlwNI CAG_nnn'CTG Cuts at: 3318 4890 3318 Size: 1572 4003 ApaI G_GGCC'C Cuts at: 2260 2260 Size: 5575 ApaLI G'TGCA_C Cuts at: 3632 4788 3632 Size: 1156 4419 ApoI r'AATT_y Cuts at: 1493 2126 2424 2884 3110 3262 5371 1493 Size: 633 298 460 226 152 2109 1697 Fragments arranged by size: 2109 1697 633 460 298 226 152 AseI AT'TA_AT Cuts at: 160 1032 4129 160 Size: 872 3097 1606 Fragments arranged by size: 3097 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 AvaI C'yCGr_G Cuts at: 1870 1943 2900 1870 Size: 73 957 4545 Fragments arranged by size: 4545 957 73 BamHI G'GATC_C Cuts at: 2437 2905 3272 2437 Size: 468 367 4740 Fragments arranged by size: 4740 468 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2363 2896 4351 618 Size: 325 217 627 576 533 1455 1842 Fragments arranged by size: 1842 1455 627 576 533 325 217 BanII G_rGCy'C Cuts at: 1391 2260 2894 1391 Size: 869 634 4072 Fragments arranged by size: 4072 869 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2226 928 Size: 971 327 4277 Fragments arranged by size: 4277 971 327 BbvCI CC'TCA_GC Cuts at: 2475 2475 Size: 5575 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3766 3800 1903 Size: 34 1829 34 3678 Fragments arranged by size: 3678 1829 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3467 4677 3467 Size: 1210 4365 BclI T'GATC_A Cuts at: 1548 2349 1548 Size: 801 4774 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4186 136 Size: 107 122 71 631 605 2514 1525 Fragments arranged by size: 2514 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5570 5570 Size: 5575 BlpI GC'TnA_GC Cuts at: 2992 2992 Size: 5575 Bme1580I G_kGCm'C Cuts at: 1790 2260 3636 4792 1790 Size: 470 1376 1156 2573 Fragments arranged by size: 2573 1376 1156 470 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5575 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2126 3306 4264 Size: 416 53 83 399 24 709 1180 958 Cuts at: 4264 442 Size: 1753 Fragments arranged by size: 1753 1180 958 709 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5575 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4235 1239 Size: 2996 2579 Bpu10I CC'TnA_GC Cuts at: 2475 2475 Size: 5575 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3700 4565 4863 3700 Size: 865 298 4412 Fragments arranged by size: 4412 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4238 882 Size: 617 2739 2219 Fragments arranged by size: 2739 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3366 493 Size: 711 2162 2702 Fragments arranged by size: 2702 2162 711 BsaBI GATnn'nnATC Cuts at: 5569 5569 Size: 5575 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3382 3764 4399 275 Size: 53 83 186 936 1849 382 635 1451 Fragments arranged by size: 1849 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4008 4406 4680 4827 5238 4008 Size: 398 274 147 411 4345 Fragments arranged by size: 4345 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2294 1178 Size: 30 165 30 861 30 4459 Fragments arranged by size: 4459 861 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2579 2882 1186 Size: 1393 303 3879 Fragments arranged by size: 3879 1393 303 BseYI C'CCAG_C Cuts at: 4778 5274 4778 Size: 496 5079 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2142 2280 2614 1163 Size: 573 406 138 334 4124 Fragments arranged by size: 4124 573 406 334 138 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3025 3786 4814 1540 Size: 275 186 1024 761 1028 2301 Fragments arranged by size: 2301 1028 1024 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3636 3721 4792 3636 Size: 85 1071 4419 Fragments arranged by size: 4419 1071 85 BsmI GAATG_Cn' Cuts at: 2126 3086 3179 2126 Size: 960 93 4522 Fragments arranged by size: 4522 960 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2864 2864 Size: 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2260 2894 3636 3721 4792 1391 Size: 399 470 634 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 634 470 399 85 BspEI T'CCGG_A Cuts at: 5238 5238 Size: 5575 BspHI T'CATG_A Cuts at: 2031 3464 5194 2031 Size: 1433 1730 2412 Fragments arranged by size: 2412 1730 1433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 844 Size: 2086 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3462 1394 Size: 429 1639 3507 Fragments arranged by size: 3507 1639 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4070 4244 65 Size: 1183 2822 174 1396 Fragments arranged by size: 2822 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4219 4406 1700 Size: 110 2409 187 2869 Fragments arranged by size: 2869 2409 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5575 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5575 BssSI C'ACGA_G Cuts at: 3635 4647 3635 Size: 1012 4563 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5575 BstBI TT'CG_AA Cuts at: 4379 4379 Size: 5575 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5575 BstYI r'GATC_y Cuts at: 1972 2437 2647 2905 3272 3657 3674 5126 5212 Size: 465 210 258 367 385 17 1452 86 Cuts at: 5212 5224 5570 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 465 385 367 346 258 210 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2652 2652 Size: 5575 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5023 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4836 Fragments arranged by size: 4836 683 56 BtsI GCAGTG_nn' Cuts at: 2518 3091 3885 3905 2518 Size: 573 794 20 4188 Fragments arranged by size: 4188 794 573 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5575 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5540 DraI TTT'AAA Cuts at: 2879 2879 Size: 5575 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2522 4582 809 Size: 507 1206 2060 1802 Fragments arranged by size: 2060 1802 1206 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3022 3911 5540 Size: 54 1425 50 275 18 1192 889 1629 Cuts at: 5540 8 Size: 43 Fragments arranged by size: 1629 1425 1192 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3022 1537 Size: 275 1210 4090 Fragments arranged by size: 4090 1210 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3505 1353 Size: 2152 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4162 4536 4682 387 Size: 1064 138 2573 374 146 1280 Fragments arranged by size: 2573 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2285 3638 4235 5022 5290 1239 Size: 393 614 39 1353 597 787 268 1524 Fragments arranged by size: 1524 1353 787 614 597 393 268 39 EcoO109I rG'GnC_Cy Cuts at: 2080 2256 2080 Size: 176 5399 EcoRI G'AATT_C Cuts at: 2884 3262 2884 Size: 378 5197 FspI TGC'GCA Cuts at: 4081 4081 Size: 5575 HaeII r_GCGC'y Cuts at: 1678 4722 1678 Size: 3044 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 2632 2664 2822 Size: 32 135 32 692 32 336 32 158 Cuts at: 2822 2854 4216 4248 4290 4322 1373 Size: 32 1362 32 42 32 2626 Fragments arranged by size: 2626 1362 692 336 158 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2919 3165 669 Size: 988 281 981 246 3079 Fragments arranged by size: 3079 988 981 281 246 HindIII A'AGCT_T Cuts at: 748 2570 748 Size: 1822 3753 HpaI GTT'AAC Cuts at: 3165 3165 Size: 5575 KpnI G_GTAC'C Cuts at: 2900 2900 Size: 5575 MfeI C'AATT_G Cuts at: 2115 3174 2115 Size: 1059 4516 MluI A'CGCG_T Cuts at: 5451 5451 Size: 5575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4689 4873 1305 Size: 3384 184 2007 Fragments arranged by size: 3384 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3753 Fragments arranged by size: 3753 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2312 3533 3892 4051 518 Size: 648 1146 1221 359 159 2042 Fragments arranged by size: 2042 1221 1146 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2343 2669 2991 3668 4816 5061 1606 Size: 737 326 322 677 1148 245 2120 Fragments arranged by size: 2120 1148 737 677 326 322 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5023 NdeI CA'TA_TG Cuts at: 387 387 Size: 5575 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5575 NotI GC'GGCC_GC Cuts at: 3022 3022 Size: 5575 NspI r_CATG'y Cuts at: 1132 1229 2469 2933 4478 1132 Size: 97 1240 464 1545 2229 Fragments arranged by size: 2229 1545 1240 464 97 PasI CC'CwG_GG Cuts at: 1348 2284 1348 Size: 936 4639 PciI A'CATG_T Cuts at: 2465 4474 2465 Size: 2009 3566 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5575 PmeI GTTT'AAAC Cuts at: 2879 2879 Size: 5575 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3644 3676 5157 5189 3644 Size: 32 1481 32 4030 Fragments arranged by size: 4030 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 2080 Size: 5575 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5575 PsiI TTA'TAA Cuts at: 3145 3145 Size: 5575 PspOMI G'GGCC_C Cuts at: 2256 2256 Size: 5575 PstI C_TGCA'G Cuts at: 830 2927 830 Size: 2097 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5575 PvuII CAG'CTG Cuts at: 2669 2669 Size: 5575 SalI G'TCGA_C Cuts at: 1936 2917 1936 Size: 981 4594 SbfI CC_TGCA'GG Cuts at: 2927 2927 Size: 5575 ScaI AGT'ACT Cuts at: 3823 5535 3823 Size: 1712 3863 SfcI C'TryA_G Cuts at: 826 1045 2245 2923 4058 4739 4930 826 Size: 219 1200 678 1135 681 191 1471 Fragments arranged by size: 1471 1200 1135 681 678 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5575 SmaI CCC'GGG Cuts at: 1872 2902 1872 Size: 1030 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3679 4580 4842 820 Size: 197 926 1736 901 262 1553 Fragments arranged by size: 1736 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3366 493 Size: 2873 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5575 SphI G_CATG'C Cuts at: 1132 2933 1132 Size: 1801 3774 SspI AAT'ATT Cuts at: 5 52 3499 5 Size: 47 3447 2081 Fragments arranged by size: 3447 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5575 StyI C'CwwG_G Cuts at: 513 1065 2076 2487 2755 3014 513 Size: 552 1011 411 268 259 3074 Fragments arranged by size: 3074 1011 552 411 268 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3602 3619 3772 3957 4402 325 Size: 1255 2022 17 153 185 445 1498 Fragments arranged by size: 2022 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3821 5533 96 Size: 274 80 33 51 1443 1844 1712 138 Fragments arranged by size: 1844 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2497 2529 5535 5567 2497 Size: 32 3006 32 2505 Fragments arranged by size: 3006 2505 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5575 XbaI T'CTAG_A Cuts at: 2911 2911 Size: 5575 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5575 XmaI C'CCGG_G Cuts at: 1870 2900 1870 Size: 1030 4545 XmnI GAAnn'nnTTC Cuts at: 2573 3704 2573 Size: 1131 4444 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3383 276 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI