Restriction map for pFN21AE5332
 (Linear) (Six-base) MAPSORT of: KIEE5332.seq  Check: 6997  from: 1  to: 870



KIEE5332.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     280     870 
   Size:        241      39     590

  Fragments arranged by size: 

                 590     241      39
 
AflIII A'CryG_T

Cuts at:      0     460     870 
   Size:        460     410
 
AleI CACnn'nnGTG

Cuts at:      0     307     870 
   Size:        307     563
 
ApaI G_GGCC'C

Cuts at:      0     255     870 
   Size:        255     615
 
ApoI r'AATT_y

Cuts at:      0     121     419     870 
   Size:        121     298     451

  Fragments arranged by size: 

                 451     298     121
 
BamHI G'GATC_C

Cuts at:      0     432     870 
   Size:        432     438
 
BanI G'GyrC_C

Cuts at:      0     358     870 
   Size:        358     512
 
BanII G_rGCy'C

Cuts at:      0     255     870 
   Size:        255     615
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     221     870 
   Size:        221     649
 
BbvCI CC'TCA_GC

Cuts at:      0     470     870 
   Size:        470     400
 
BclI T'GATC_A

Cuts at:      0     344     870 
   Size:        344     526
 
Bme1580I G_kGCm'C

Cuts at:      0     255     870 
   Size:        255     615
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     121     870 
   Size:        121     749
 
Bpu10I CC'TnA_GC

Cuts at:      0     470     870 
   Size:        470     400
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     259     289     870 
   Size:        259      30     581

  Fragments arranged by size: 

                 581     259      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     574     870 
   Size:        574     296
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     275     609     870 
   Size:        137     138     334     261

  Fragments arranged by size: 

                 334     261     138     137
 
BsmI GAATG_Cn'

Cuts at:      0     121     870 
   Size:        121     749
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     859     870 
   Size:        859      11
 
Bsp1286I G_dGCh'C

Cuts at:      0     255     870 
   Size:        255     615
 
BspHI T'CATG_A

Cuts at:      0      26     870 
   Size:         26     844
 
BstYI r'GATC_y

Cuts at:      0     432     642     870 
   Size:        432     210     228

  Fragments arranged by size: 

                 432     228     210
 
Bsu36I CC'TnA_GG

Cuts at:      0     647     870 
   Size:        647     223
 
BtsI GCAGTG_nn'

Cuts at:      0     513     870 
   Size:        513     357
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     517     870 
   Size:        517     353
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     280     870 
   Size:        241      39     590

  Fragments arranged by size: 

                 590     241      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75     251     870 
   Size:         75     176     619

  Fragments arranged by size: 

                 619     176      75
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     259     291     627     659     817     849     870 
   Size:        259      32     336      32     158      32      21

  Fragments arranged by size: 

                 336     259     158      32      32      32      21
 
HindIII A'AGCT_T

Cuts at:      0     565     870 
   Size:        565     305
 
MfeI C'AATT_G

Cuts at:      0     110     870 
   Size:        110     760
 
MslI CAynn'nnrTG

Cuts at:      0     307     870 
   Size:        307     563
 
MspA1I CmG'CkG

Cuts at:      0     338     664     870 
   Size:        338     326     206

  Fragments arranged by size: 

                 338     326     206
 
NspI r_CATG'y

Cuts at:      0     464     870 
   Size:        464     406
 
PasI CC'CwG_GG

Cuts at:      0     279     870 
   Size:        279     591
 
PciI A'CATG_T

Cuts at:      0     460     870 
   Size:        460     410
 
PpuMI rG'GwC_Cy

Cuts at:      0      75     870 
   Size:         75     795
 
PspOMI G'GGCC_C

Cuts at:      0     251     870 
   Size:        251     619
 
PvuII CAG'CTG

Cuts at:      0     664     870 
   Size:        664     206
 
SfcI C'TryA_G

Cuts at:      0     240     870 
   Size:        240     630
 
StyI C'CwwG_G

Cuts at:      0      71     482     750     870 
   Size:         71     411     268     120

  Fragments arranged by size: 

                 411     268     120      71
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     492     524     870 
   Size:        492      32     346

  Fragments arranged by size: 

                 492     346      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     568     870 
   Size:        568     302
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApaI     ApoI    BamHI     BanI    BanII
     BbsI    BbvCI     BclI Bme1580I     BmrI   Bpu10I    BsaXI    BseRI
     BsgI     BsmI    BsmBI Bsp1286I    BspHI    BstYI   Bsu36I     BtsI
     DrdI  Eco57MI EcoO109I    Hin4I  HindIII     MfeI     MslI   MspA1I
     NspI     PasI     PciI    PpuMI   PspOMI    PvuII     SfcI     StyI
     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BbeI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com