################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII AccI AclI AflII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII BbrPI BbvCI BcgI BclI BfrI BglI BsiEI BsiWI BsoBI BspDI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI FbaI FseI FspI KspI MfeI MluI MslI MunI NdeI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 986 GGTACC G^GTACC AccIII 1013 TCCGGA T^CCGGA AcsI 1563 AAATTT [GA]^AATT[CT] AflIII 2097 ACACGT A^C[GA][CT]GT AlwNI 596 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 2192 AGCGCT AGC^GCT ApaI 2542 GGGCCC GGGCC^C ApoI 1563 AAATTT [GA]^AATT[CT] Asp700I 2755 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 986 GGTACC G^GTACC AvrII 2383 CCTAGG C^CTAGG BamHI 1010 GGATCC G^GATCC BbeI 632 GGCGCC GGCGC^C BciVI 931 GGATAC GTATCC(6/5) BlnI 2383 CCTAGG C^CTAGG BlpI 3031 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3031 GCTCAGC GC^T[ACGT]AGC BsaAI 2104 CACGTA [CT]AC^GT[GA] BsaBI 2152 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1013 TCCGGA [AT]^CCGG[AT] BseAI 1013 TCCGGA T^CCGGA BsmI 1469 GCATTC GAATGC(1/-1) BspEI 1013 TCCGGA T^CCGGA BsrFI 920 GCCGGC [GA]^CCGG[CT] BstAPI 2653 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2828 CCACAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 3031 GCTCAGC GC^T[ACGT]AGC Cfr10I 920 GCCGGC [GA]^CCGG[CT] DraIII 421 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2192 AGCGCT AGC^GCT EcoNI 2869 CCTAAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 940 ATGCAT ATGCA^T HindIII 1960 AAGCTT A^AGCTT HpaI 1996 GTTAAC GTT^AAC KasI 632 GGCGCC G^GCGCC KpnI 986 GGTACC GGTAC^C MamI 2152 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1013 TCCGGA T^CCGGA NaeI 920 GCCGGC GCC^GGC NarI 632 GGCGCC GG^CGCC NcoI 1259 CCATGG C^CATGG NgoMIV 920 GCCGGC G^CCGGC NheI 2994 GCTAGC G^CTAGC NsiI 940 ATGCAT ATGCA^T PflMI 2800 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 3253 GAGCTC GAGCT^C SfoI 632 GGCGCC GGC^GCC SphI 938 GCATGC GCATG^C StuI 2476 AGGCCT AGG^CCT Van91I 2800 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 189 TCTAGA T^CTAGA XmnI 2755 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 143 CGCG CG^CG 348 CGCG BalI 1517 TGGCCA TGG^CCA 3318 TGGCCA BanII 2542 GGGCCC G[GA]GC[CT]^C 3253 GAGCTC BcnI 2393 CCGGG CC^[GC]GG 2494 CCCGG BsmBI 3098 GAGACG CGTCTC(1/5) 3157 GAGACG BstUI 143 CGCG CG^CG 348 CGCG EaeI 1517 TGGCCA [CT]^GGCC[GA] 3318 TGGCCA EcoRV 1161 GATATC GAT^ATC 1221 GATATC FauI 2748 CCCGC CCCGC(4/6) 1285 GCGGG HaeII 632 GGCGCC [GA]GCGC^[CT] 2192 AGCGCT HgaI 3100 GACGC GACGC(5/10) 3159 GACGC HincII 1858 GTTGAC GT[CT]^[GA]AC 1996 GTTAAC HindII 1858 GTTGAC GT[CT]^[GA]AC 1996 GTTAAC MluNI 1517 TGGCCA TGG^CCA 3318 TGGCCA MscI 1517 TGGCCA TGG^CCA 3318 TGGCCA MvnI 143 CGCG CG^CG 348 CGCG NciI 2393 CCGGG CC^[GC]GG 2494 CCCGG NspI 938 GCATGC [GA]CATG^[CT] 1071 ACATGC PpuMI 2906 GGGACCT [GA]G^G[AT]CC[CT] 2960 AGGACCT SmlI 484 CTCAAG C^T[CT][GA]AG 1702 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AspHI AvaII BanI BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII