################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeII MaeIII MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AcsI 591 AAATTT [GA]^AATT[CT] ApaI 398 GGGCCC GGGCC^C ApoI 591 AAATTT [GA]^AATT[CT] AvaII 193 GGTCC G^G[AT]CC BanII 398 GGGCCC G[GA]GC[CT]^C BfaI 674 CTAG C^TAG BlpI 338 GCTTAGC GC^T[ACGT]AGC Bpu1102I 338 GCTTAGC GC^T[ACGT]AGC BsaBI 318 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 746 GAGACC GGTCTC(1/5) BsrDI 343 GCAATG GCAATG(2/0) BstF5I 30 CATCC GGATG(2/0) CelII 338 GCTTAGC GC^T[ACGT]AGC CfoI 45 GCGC GCG^C EarI 115 CTCTTC CTCTTC(1/4) FauI 710 GCGGG CCCGC(4/6) FokI 30 CATCC GGATG(9/13) HhaI 45 GCGC GCG^C HinP1I 45 GCGC G^CGC Ksp632I 115 CTCTTC CTCTTC(1/4) MaeI 674 CTAG C^TAG MamI 318 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NheI 673 GCTAGC G^CTAGC PflMI 196 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 403 CAGCTG CAG^CTG ScaI 384 AGTACT AGT^ACT StuI 452 AGGCCT AGG^CCT TaqI 738 TCGA T^CGA TspRI 168 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 738 TCGA T^CGA Van91I 196 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 44 CGCG CG^CG 251 CGCG AfaI 385 GTAC GT^AC 618 GTAC AlwNI 197 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 355 CAGTTGCTG BbvI 627 GCTGC GCAGC(8/12) 688 GCTGC BclI 374 TGATCA T^GATCA 693 TGATCA BcnI 33 CCCGG CC^[GC]GG 105 CCCGG BsiYI 76 CCCTTGGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 196 CCAGGAACTGG BslI 76 CCCTTGGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 196 CCAGGAACTGG BstUI 44 CGCG CG^CG 251 CGCG DdeI 339 CTTAG C^T[ACGT]AG 608 CTCAG EcoT14I 77 CCTTGG C^C[AT][AT]GG 477 CCTTGG FbaI 374 TGATCA T^GATCA 693 TGATCA HapII 34 CCGG C^CGG 106 CCGG HinfI 366 GATTC G^A[ACGT]TC 482 GATTC HpaII 34 CCGG C^CGG 106 CCGG MboII 587 GAAGA GAAGA(8/7) 116 TCTTC MseI 311 TTAA T^TAA 449 TTAA MspA1I 403 CAGCTG C[AC]G^C[GT]G 621 CCGCTG MspI 34 CCGG C^CGG 106 CCGG MvnI 44 CGCG CG^CG 251 CGCG NciI 33 CCCGG CC^[GC]GG 105 CCCGG RsaI 385 GTAC GT^AC 618 GTAC SfaNI 29 GCATC GCATC(5/9) 323 GCATC StyI 77 CCTTGG C^C[AT][AT]GG 477 CCTTGG TfiI 366 GATTC G^A[AT]TC 482 GATTC Tru9I 311 TTAA T^TAA 449 TTAA TseI 627 GCTGC G^C[AT]GC 688 GCTGC Tsp509I 223 AATT ^AATT 592 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AluI BmyI BsaJI BseRI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI HaeIII ItaI MboI MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI