Restriction map for pFN21AE5237
 (Circular) (Six-base) MAPSORT of: pFN21AE5237.seq  Check: 5932  from: 1  to: 5458



pFN21AE5237  5458 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:15  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3268     278 
   Size:         53      83     186     936    1732    2468

  Fragments arranged by size: 

                2468    1732     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2801    1937 
   Size:        864    4594
 
Acc65I G'GTAC_C

Cuts at:   2779    2779 
   Size:       5458
 
AclI AA'CG_TT

Cuts at:   1563    3585    3958    1563 
   Size:       2022     373    3063

  Fragments arranged by size: 

                3063    2022     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3521    4905    5173    1632 
   Size:       1889    1384     268    1917

  Fragments arranged by size: 

                1917    1889    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5261
 
AflIII A'CryG_T

Cuts at:   1203    4357    5334    1203 
   Size:       3154     977    1327

  Fragments arranged by size: 

                3154    1327     977
 
AgeI A'CCGG_T

Cuts at:   4289    4289 
   Size:       5458
 
AhdI GACnn_n'nnGTC

Cuts at:   4187    4187 
   Size:       5458
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5458
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5458
 
AlwNI CAG_nnn'CTG

Cuts at:   2207    2365    3201    4773    2207 
   Size:        158     836    1572    2892

  Fragments arranged by size: 

                2892    1572     836     158
 
ApaI G_GGCC'C

Cuts at:   2407    2407 
   Size:       5458
 
ApaLI G'TGCA_C

Cuts at:   3515    4671    3515 
   Size:       1156    4302
 
ApoI r'AATT_y

Cuts at:   1493    2596    2767    2993    3145    5254    1493 
   Size:       1103     171     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1103     226     171     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4012     160 
   Size:        872    2980    1606

  Fragments arranged by size: 

                2980    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5458
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2783    1870 
   Size:         73     840    4545

  Fragments arranged by size: 

                4545     840      73
 
BamHI G'GATC_C

Cuts at:   2788    3155    2788 
   Size:        367    5091
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2779    4234     618 
   Size:        325     217     627     992    1455    1842

  Fragments arranged by size: 

                1842    1455     992     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2407    2777    1391 
   Size:       1016     370    4072

  Fragments arranged by size: 

                4072    1016     370
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4487
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3649    3683    1903 
   Size:         34    1712      34    3678

  Fragments arranged by size: 

                3678    1712      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3350    4560    3350 
   Size:       1210    4248
 
BclI T'GATC_A

Cuts at:   1548    2379    2698    1548 
   Size:        831     319    4308

  Fragments arranged by size: 

                4308     831     319
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4069     136 
   Size:        107     122      71     631     605    2397    1525

  Fragments arranged by size: 

                2397    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5453    5453 
   Size:       5458
 
BlpI GC'TnA_GC

Cuts at:   2344    2875    2344 
   Size:        531    4927
 
Bme1580I G_kGCm'C

Cuts at:   1790    2082    2407    2673    3519    4675    1790 
   Size:        292     325     266     846    1156    2573

  Fragments arranged by size: 

                2573    1156     846     325     292     266
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5458
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2631    2737    3189 
   Size:        416      53      83     399      24    1214     106     452

Cuts at:   3189    4147     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1214     958     452     416     399     106      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    2682    1056 
   Size:       1626    3832
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4118    1239 
   Size:       2879    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3583    4448    4746    3583 
   Size:        865     298    4295

  Fragments arranged by size: 

                4295     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2745    4121     882 
   Size:        617    1246    1376    2219

  Fragments arranged by size: 

                2219    1376    1246     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3249     493 
   Size:        711    2045    2702

  Fragments arranged by size: 

                2702    2045     711
 
BsaBI GATnn'nnATC

Cuts at:   2327    5452    2327 
   Size:       3125    2333
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3265    3647    4282     275 
   Size:         53      83     186     936    1732     382     635    1451

  Fragments arranged by size: 

                1732    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3891    4289    4563    4710    5121    3891 
   Size:        398     274     147     411    4228

  Fragments arranged by size: 

                4228     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2496    2526    2742    2772    1178 
   Size:         30     165      30    1093      30     216      30    3864

  Fragments arranged by size: 

                3864    1093     216     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2300    2661    2664    1186 
   Size:       1114     361       3    3980

  Fragments arranged by size: 

                3980    1114     361       3
 
BseYI C'CCAG_C

Cuts at:   2406    4661    5157    2406 
   Size:       2255     496    2707

  Fragments arranged by size: 

                2707    2255     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4885
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2908    3669    4697    1540 
   Size:        275     186     907     761    1028    2301

  Fragments arranged by size: 

                2301    1028     907     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3519    3604    4675    3519 
   Size:         85    1071    4302

  Fragments arranged by size: 

                4302    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2969    3062    2969 
   Size:         93    5365
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2082    2407    2673    2777    3519    3604    4675 
   Size:        399     292     325     266     104     742      85    1071

Cuts at:   4675    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     399     325     292     266     104
                  85
 
BspEI T'CCGG_A

Cuts at:   5121    5121 
   Size:       5458
 
BspHI T'CATG_A

Cuts at:   3347    5077    3347 
   Size:       1730    3728
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2813     844 
   Size:       1969    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3345    1394 
   Size:        429    1522    3507

  Fragments arranged by size: 

                3507    1522     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2355    3953    4127      65 
   Size:       1183    1107    1598     174    1396

  Fragments arranged by size: 

                1598    1396    1183    1107     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4102    4289    1700 
   Size:        110    2292     187    2869

  Fragments arranged by size: 

                2869    2292     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5458
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5458
 
BssSI C'ACGA_G

Cuts at:   3518    4530    3518 
   Size:       1012    4446
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5458
 
BstBI TT'CG_AA

Cuts at:   4262    4262 
   Size:       5458
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5458
 
BstYI r'GATC_y

Cuts at:   1972    2788    3155    3540    3557    5009    5095    5107    5453 
   Size:        816     367     385      17    1452      86      12     346

Cuts at:   5453    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     816     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4906
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4719

  Fragments arranged by size: 

                4719     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2172    2974    3768    3788    2172 
   Size:        802     794      20    3842

  Fragments arranged by size: 

                3842     802     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5458
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5423
 
DraI TTT'AAA

Cuts at:   2762    2762 
   Size:       5458
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4465     809 
   Size:        507    3149    1802

  Fragments arranged by size: 

                3149    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2905    3794    5423 
   Size:         54    1425      50     275      18    1075     889    1629

Cuts at:   5423       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1075     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2905    1537 
   Size:        275    1093    4090

  Fragments arranged by size: 

                4090    1093     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2126    3388    1353 
   Size:        773    1262    3423

  Fragments arranged by size: 

                3423    1262     773
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2724    4045    4419    4565     387 
   Size:       1064     138    1135    1321     374     146    1280

  Fragments arranged by size: 

                1321    1280    1135    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3521    4118    4905    5173    1239 
   Size:        393    1889     597     787     268    1524

  Fragments arranged by size: 

                1889    1524     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2767    3145    2767 
   Size:        378    5080
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2159    2159 
   Size:       5458
 
FspI TGC'GCA

Cuts at:   3964    3964 
   Size:       5458
 
HaeII r_GCGC'y

Cuts at:   1678    4605    1678 
   Size:       2927    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2359    2391    4099    4131    4173 
   Size:         32     135      32     787      32    1708      32      42

Cuts at:   4173    4205    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1708     787     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2802    3048     669 
   Size:        988     281     864     246    3079

  Fragments arranged by size: 

                3079     988     864     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5458
 
HpaI GTT'AAC

Cuts at:   3048    3048 
   Size:       5458
 
KpnI G_GTAC'C

Cuts at:   2783    2783 
   Size:       5458
 
MfeI C'AATT_G

Cuts at:   3057    3057 
   Size:       5458
 
MluI A'CGCG_T

Cuts at:   5334    5334 
   Size:       5458
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    1989    2521    4572    4756    1305 
   Size:        684     532    2051     184    2007

  Fragments arranged by size: 

                2051    2007     684     532     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3636

  Fragments arranged by size: 

                3636    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3416    3775    3934     518 
   Size:        648    2250     359     159    2042

  Fragments arranged by size: 

                2250    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2410    2628    2874    3551    4699    4944    1606 
   Size:        804     218     246     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     804     677     246     245     218
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4906
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5458
 
NheI G'CTAG_C

Cuts at:   1052    2678    1052 
   Size:       1626    3832
 
NotI GC'GGCC_GC

Cuts at:   2905    2905 
   Size:       5458
 
NspI r_CATG'y

Cuts at:   1132    1229    2816    4361    1132 
   Size:         97    1587    1545    2229

  Fragments arranged by size: 

                2229    1587    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2472    1348 
   Size:       1124    4334
 
PciI A'CATG_T

Cuts at:   4357    4357 
   Size:       5458
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2207    1108 
   Size:       1099    4359
 
PfoI T'CCnGG_A

Cuts at:   2200    2200 
   Size:       5458
 
PmeI GTTT'AAAC

Cuts at:   2762    2762 
   Size:       5458
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3527    3559    5040    5072    3527 
   Size:         32    1481      32    3913

  Fragments arranged by size: 

                3913    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5458
 
PsiI TTA'TAA

Cuts at:   3028    3028 
   Size:       5458
 
PspOMI G'GGCC_C

Cuts at:   2403    2403 
   Size:       5458
 
PstI C_TGCA'G

Cuts at:    830    2810     830 
   Size:       1980    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5458
 
PvuII CAG'CTG

Cuts at:   2410    2410 
   Size:       5458
 
SalI G'TCGA_C

Cuts at:   1936    2800    1936 
   Size:        864    4594
 
SbfI CC_TGCA'GG

Cuts at:   2810    2810 
   Size:       5458
 
ScaI AGT'ACT

Cuts at:   2391    3706    5418    2391 
   Size:       1315    1712    2431

  Fragments arranged by size: 

                2431    1712    1315
 
SexAI A'CCwGG_T

Cuts at:   2754    2754 
   Size:       5458
 
SfcI C'TryA_G

Cuts at:    826    1045    2806    3941    4622    4813     826 
   Size:        219    1761    1135     681     191    1471

  Fragments arranged by size: 

                1761    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5458
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5458
 
SmaI CCC'GGG

Cuts at:   1872    2785    1872 
   Size:        913    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3562    4463    4725     820 
   Size:        197     926    1619     901     262    1553

  Fragments arranged by size: 

                1619    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3249     493 
   Size:       2756    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5458
 
SphI G_CATG'C

Cuts at:   1132    2816    1132 
   Size:       1684    3774
 
SspI AAT'ATT

Cuts at:      5      52    3382       5 
   Size:         47    3330    2081

  Fragments arranged by size: 

                3330    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2459    1517 
   Size:        942    4516
 
StyI C'CwwG_G

Cuts at:    513    1065    2082    2482    2897     513 
   Size:        552    1017     400     415    3074

  Fragments arranged by size: 

                3074    1017     552     415     400
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3485    3502    3655    3840    4285     325 
   Size:       1255    1905      17     153     185     445    1498

  Fragments arranged by size: 

                1905    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2389    3704    5416 
   Size:        274      80      33      51    1443     412    1315    1712

Cuts at:   5416      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1315     412     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2652    2684    5418    5450    2652 
   Size:         32    2734      32    2660

  Fragments arranged by size: 

                2734    2660      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5458
 
XbaI T'CTAG_A

Cuts at:   2794    2794 
   Size:       5458
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5458
 
XmaI C'CCGG_G

Cuts at:   1870    2783    1870 
   Size:        913    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3587    3587 
   Size:       5458
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3266     276 
   Size:         53      83     186     936    1732    2468

  Fragments arranged by size: 

                2468    1732     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII
     SalI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI
    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI     SfiI
     SfoI     SrfI     SwaI     XcmI
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