################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsgI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstZ17I Bsu36I CelII ClaI DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I MaeII MaeIII MamI MfeI MluI MroI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI SalI SapI SbfI SexAI SfaNI SfiI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcyI 108 GGCGCC G[GA]^CG[CT]C AfaI 213 GTAC GT^AC AlwI 483 GGATC GGATC(4/5) AspHI 98 GAGCTC G[AT]GC[AT]^C AvaI 93 CCCGGG C^[CT]CG[GA]G BalI 521 TGGCCA TGG^CCA BbeI 108 GGCGCC GGCGC^C BbvCI 121 CCTCAGC CCTCAGC(-5/-2) BfaI 410 CTAG C^TAG BsaHI 108 GGCGCC G[GA]^CG[CT]C BsaWI 441 ACCGGA [AT]^CCGG[AT] BsiHKAI 98 GAGCTC G[AT]GC[AT]^C BsmFI 91 GTCCC GGGAC(10/14) BsmI 414 GAATGC GAATGC(1/-1) BsoBI 93 CCCGGG C^[CT]CG[GA]G BsrBI 251 CCGCTC CCGCTC(-3/-3) BstXI 473 CCATCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 483 GGATCT [GA]^GATC[CT] CpoI 457 CGGTCCG CG^G[AT]CCG DpnII 484 GATC ^GATC EcoT14I 128 CCTTGG C^C[AT][AT]GG Hin1I 108 GGCGCC G[GA]^CG[CT]C HinfI 29 GAGTC G^A[ACGT]TC HphI 488 TCACC GGTGA(8/7) KasI 108 GGCGCC G^GCGCC MaeI 410 CTAG C^TAG MboI 484 GATC ^GATC MboII 137 TCTTC GAAGA(8/7) MflI 483 GGATCT [GA]^GATC[CT] MluNI 521 TGGCCA TGG^CCA MscI 521 TGGCCA TGG^CCA NaeI 283 GCCGGC GCC^GGC NarI 108 GGCGCC GG^CGCC NdeII 484 GATC ^GATC NgoMIV 283 GCCGGC G^CCGGC PleI 29 GAGTC GAGTC(4/5) PstI 346 CTGCAG CTGCA^G PvuII 352 CAGCTG CAG^CTG RsaI 213 GTAC GT^AC RsrII 457 CGGTCCG CG^G[AT]CCG SacI 98 GAGCTC GAGCT^C Sau3AI 484 GATC ^GATC ScaI 212 AGTACT AGT^ACT SfcI 346 CTGCAG C^T[GA][CT]AG SfoI 108 GGCGCC GGC^GCC SgrAI 282 CGCCGGCG C[GA]^CCGG[CT]G SmaI 93 CCCGGG CCC^GGG StyI 128 CCTTGG C^C[AT][AT]GG TaqI 377 TCGA T^CGA TthHB8I 377 TCGA T^CGA XhoII 483 GGATCT [GA]^GATC[CT] XmaCI 93 CCCGGG C^CCGGG XmaI 93 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AlwNI 349 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 352 CAGCTGCTG BanI 108 GGCGCC G^G[CT][GA]CC 438 GGCACC BanII 98 GAGCTC G[GA]GC[CT]^C 243 GAGCCC BmyI 98 GAGCTC G[AGT]GC[ACT]^C 243 GAGCCC Bsp1286I 98 GAGCTC G[AGT]GC[ACT]^C 243 GAGCCC BstAPI 348 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 351 GCAGCTGCTGC DdeI 76 CTGAG C^T[ACGT]AG 122 CTCAG EagI 184 CGGCCG C^GGCCG 248 CGGCCG EclXI 184 CGGCCG C^GGCCG 248 CGGCCG Eco52I 184 CGGCCG C^GGCCG 248 CGGCCG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AccII AciI AluI AvaII BbvI BcnI BsaJI BseRI BsiEI BsiYI BslI BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DsaI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI KspI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV SacII Sau96I ScrFI TseI