(Circular) (Six-base) MAPSORT of: pFN21AE1683.seq Check: 7957 from: 1 to: 5293 pFN21AE1683 5293 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3103 278 Size: 53 83 186 936 1567 2468 Fragments arranged by size: 2468 1567 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2636 1937 Size: 699 4594 Acc65I G'GTAC_C Cuts at: 2614 2614 Size: 5293 AclI AA'CG_TT Cuts at: 1563 3420 3793 1563 Size: 1857 373 3063 Fragments arranged by size: 3063 1857 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3356 4740 5008 1632 Size: 1724 1384 268 1917 Fragments arranged by size: 1917 1724 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5096 AflIII A'CryG_T Cuts at: 1203 4192 5169 1203 Size: 2989 977 1327 Fragments arranged by size: 2989 1327 977 AgeI A'CCGG_T Cuts at: 4124 4124 Size: 5293 AhdI GACnn_n'nnGTC Cuts at: 4022 4022 Size: 5293 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5293 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5293 AlwNI CAG_nnn'CTG Cuts at: 2359 2362 3036 4608 2359 Size: 3 674 1572 3044 Fragments arranged by size: 3044 1572 674 3 ApaLI G'TGCA_C Cuts at: 3350 4506 3350 Size: 1156 4137 ApoI r'AATT_y Cuts at: 1493 2602 2828 2980 5089 1493 Size: 1109 226 152 2109 1697 Fragments arranged by size: 2109 1697 1109 226 152 AseI AT'TA_AT Cuts at: 160 1032 3847 160 Size: 872 2815 1606 Fragments arranged by size: 2815 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5293 AvaI C'yCGr_G Cuts at: 1870 1943 2098 2618 1870 Size: 73 155 520 4545 Fragments arranged by size: 4545 520 155 73 BamHI G'GATC_C Cuts at: 2623 2990 2623 Size: 367 4926 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2113 2443 2614 4069 618 Size: 325 217 627 326 330 171 1455 1842 Fragments arranged by size: 1842 1455 627 330 326 325 217 171 BanII G_rGCy'C Cuts at: 1391 2107 2252 2612 1391 Size: 716 145 360 4072 Fragments arranged by size: 4072 716 360 145 BbeI G_GCGC'C Cuts at: 2117 2117 Size: 5293 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4322 BbvCI CC'TCA_GC Cuts at: 2127 2127 Size: 5293 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3484 3518 1903 Size: 34 50 34 1463 34 3678 Fragments arranged by size: 3678 1463 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3185 4395 3185 Size: 1210 4083 BclI T'GATC_A Cuts at: 1548 1548 Size: 5293 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3904 136 Size: 107 122 71 631 605 2232 1525 Fragments arranged by size: 2232 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5288 5288 Size: 5293 BlpI GC'TnA_GC Cuts at: 2710 2710 Size: 5293 Bme1580I G_kGCm'C Cuts at: 1790 3354 4510 1790 Size: 1564 1156 2573 Fragments arranged by size: 2573 1564 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5293 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3024 3982 442 Size: 416 53 83 399 24 1607 958 1753 Fragments arranged by size: 1753 1607 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5293 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3953 1239 Size: 2714 2579 Bpu10I CC'TnA_GC Cuts at: 2081 2127 2081 Size: 46 5247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3418 4283 4581 3418 Size: 865 298 4130 Fragments arranged by size: 4130 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3956 882 Size: 617 2457 2219 Fragments arranged by size: 2457 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3084 493 Size: 711 1880 2702 Fragments arranged by size: 2702 1880 711 BsaBI GATnn'nnATC Cuts at: 5287 5287 Size: 5293 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2114 3100 3482 4117 Size: 53 83 186 936 581 986 382 635 Cuts at: 4117 275 Size: 1451 Fragments arranged by size: 1451 986 936 635 581 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2446 3726 4124 4398 4545 4956 2446 Size: 1280 398 274 147 411 2783 Fragments arranged by size: 2783 1280 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5068 Fragments arranged by size: 5068 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 2266 2273 2483 2495 1186 Size: 1042 38 7 210 12 3984 Fragments arranged by size: 3984 1042 210 38 12 7 BseYI C'CCAG_C Cuts at: 2172 4496 4992 2172 Size: 2324 496 2473 Fragments arranged by size: 2473 2324 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4720 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2192 2256 2328 2743 3504 4532 Size: 275 186 191 64 72 415 761 1028 Cuts at: 4532 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 415 275 191 186 72 64 BsiHKAI G_wGCw'C Cuts at: 2107 3354 3439 4510 2107 Size: 1247 85 1071 2890 Fragments arranged by size: 2890 1247 1071 85 BsmI GAATG_Cn' Cuts at: 2425 2804 2897 2425 Size: 379 93 4821 Fragments arranged by size: 4821 379 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 2252 2612 3354 3439 4510 1391 Size: 399 317 145 360 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 360 317 145 85 BspEI T'CCGG_A Cuts at: 4956 4956 Size: 5293 BspHI T'CATG_A Cuts at: 3182 4912 3182 Size: 1730 3563 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2648 844 Size: 1804 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2258 3180 1394 Size: 429 435 922 3507 Fragments arranged by size: 3507 922 435 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3788 3962 65 Size: 1183 2540 174 1396 Fragments arranged by size: 2540 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 2191 2288 3937 4124 1700 Size: 110 282 99 97 1649 187 2869 Fragments arranged by size: 2869 1649 282 187 110 99 97 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5293 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5293 BssSI C'ACGA_G Cuts at: 3353 4365 3353 Size: 1012 4281 BstAPI GCAn_nnn'nTGC Cuts at: 794 2359 2362 794 Size: 1565 3 3725 Fragments arranged by size: 3725 1565 3 BstBI TT'CG_AA Cuts at: 4097 4097 Size: 5293 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5293 BstXI CCAn_nnnn'nTGG Cuts at: 2485 2485 Size: 5293 BstYI r'GATC_y Cuts at: 1972 2488 2623 2990 3375 3392 4844 4930 4942 Size: 516 135 367 385 17 1452 86 12 Cuts at: 4942 5288 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 516 385 367 346 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2028 2086 2391 513 Size: 552 963 58 305 3415 Fragments arranged by size: 3415 963 552 305 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4554 Fragments arranged by size: 4554 683 56 BtsI GCAGTG_nn' Cuts at: 2809 3603 3623 2809 Size: 794 20 4479 Fragments arranged by size: 4479 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5293 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5258 DraI TTT'AAA Cuts at: 2597 2597 Size: 5293 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4300 809 Size: 507 2984 1802 Fragments arranged by size: 2984 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2189 2253 2431 Size: 54 1425 50 275 18 359 64 178 Cuts at: 2431 2526 2740 3629 5258 8 Size: 95 214 889 1629 43 Fragments arranged by size: 1629 1425 889 359 275 214 178 95 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2189 2253 2740 1537 Size: 275 377 64 487 4090 Fragments arranged by size: 4090 487 377 275 64 EarI CTCTTCn'nnn_ Cuts at: 1353 3223 1353 Size: 1870 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2216 3880 4254 4400 387 Size: 1064 138 627 1664 374 146 1280 Fragments arranged by size: 1664 1280 1064 627 374 146 138 EcoICRI GAG'CTC Cuts at: 2105 2105 Size: 5293 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3356 3953 4740 5008 1239 Size: 393 1724 597 787 268 1524 Fragments arranged by size: 1724 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2602 2980 2602 Size: 378 4915 FspI TGC'GCA Cuts at: 3799 3799 Size: 5293 HaeII r_GCGC'y Cuts at: 1678 2078 2117 2377 2415 4440 1678 Size: 400 39 260 38 2025 2531 Fragments arranged by size: 2531 2025 400 260 39 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3934 3966 4008 4040 1373 Size: 32 135 32 2362 32 42 32 2626 Fragments arranged by size: 2626 2362 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2637 2883 669 Size: 988 281 699 246 3079 Fragments arranged by size: 3079 988 699 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5293 HpaI GTT'AAC Cuts at: 2883 2883 Size: 5293 KasI G'GCGC_C Cuts at: 2113 2113 Size: 5293 KpnI G_GTAC'C Cuts at: 2618 2618 Size: 5293 MfeI C'AATT_G Cuts at: 2892 2892 Size: 5293 MluI A'CGCG_T Cuts at: 5169 5169 Size: 5293 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4407 4591 1305 Size: 3102 184 2007 Fragments arranged by size: 3102 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2528 10 Size: 54 1425 343 696 2775 Fragments arranged by size: 2775 1425 696 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3251 3610 3769 518 Size: 648 2085 359 159 2042 Fragments arranged by size: 2085 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2030 2088 2298 2359 2393 2709 3386 Size: 411 13 58 210 61 34 316 677 Cuts at: 3386 4534 4779 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 411 316 245 210 61 58 34 13 NaeI GCC'GGC Cuts at: 2290 2290 Size: 5293 NarI GG'CG_CC Cuts at: 2114 2114 Size: 5293 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4741 NdeI CA'TA_TG Cuts at: 387 387 Size: 5293 NgoMIV G'CCGG_C Cuts at: 2288 2288 Size: 5293 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5293 NotI GC'GGCC_GC Cuts at: 2740 2740 Size: 5293 NspI r_CATG'y Cuts at: 1132 1229 2651 4196 1132 Size: 97 1422 1545 2229 Fragments arranged by size: 2229 1545 1422 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5293 PciI A'CATG_T Cuts at: 4192 4192 Size: 5293 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5293 PfoI T'CCnGG_A Cuts at: 2508 2508 Size: 5293 PmeI GTTT'AAAC Cuts at: 2597 2597 Size: 5293 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3362 3394 4875 4907 3362 Size: 32 1481 32 3748 Fragments arranged by size: 3748 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5293 PsiI TTA'TAA Cuts at: 2863 2863 Size: 5293 PstI C_TGCA'G Cuts at: 830 2355 2645 830 Size: 1525 290 3478 Fragments arranged by size: 3478 1525 290 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5293 PvuII CAG'CTG Cuts at: 2359 2359 Size: 5293 RsrII CG'GwC_CG Cuts at: 2463 2463 Size: 5293 SacI G_AGCT'C Cuts at: 2107 2107 Size: 5293 SacII CC_GC'GG Cuts at: 2031 2089 2394 2031 Size: 58 305 4930 Fragments arranged by size: 4930 305 58 SalI G'TCGA_C Cuts at: 1936 2635 1936 Size: 699 4594 SbfI CC_TGCA'GG Cuts at: 2645 2645 Size: 5293 ScaI AGT'ACT Cuts at: 2219 3541 5253 2219 Size: 1322 1712 2259 Fragments arranged by size: 2259 1712 1322 SfcI C'TryA_G Cuts at: 826 1045 2351 2641 3776 4457 4648 826 Size: 219 1306 290 1135 681 191 1471 Fragments arranged by size: 1471 1306 1135 681 290 219 191 SfoI GGC'GCC Cuts at: 2115 2115 Size: 5293 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5293 SgrAI Cr'CCGG_yG Cuts at: 1700 2288 1700 Size: 588 4705 SmaI CCC'GGG Cuts at: 1872 2100 2620 1872 Size: 228 520 4545 Fragments arranged by size: 4545 520 228 SmlI C'TyrA_G Cuts at: 820 1017 1943 3397 4298 4560 820 Size: 197 926 1454 901 262 1553 Fragments arranged by size: 1553 1454 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3084 493 Size: 2591 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5293 SphI G_CATG'C Cuts at: 1132 2651 1132 Size: 1519 3774 SspI AAT'ATT Cuts at: 5 52 3217 5 Size: 47 3165 2081 Fragments arranged by size: 3165 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5293 StyI C'CwwG_G Cuts at: 513 1065 2133 2732 513 Size: 552 1068 599 3074 Fragments arranged by size: 3074 1068 599 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3320 3337 3490 3675 4120 325 Size: 1255 1740 17 153 185 445 1498 Fragments arranged by size: 1740 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2217 3539 5251 Size: 274 80 33 51 1443 240 1322 1712 Cuts at: 5251 96 Size: 138 Fragments arranged by size: 1712 1443 1322 274 240 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5253 5285 5253 Size: 32 5261 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5293 XbaI T'CTAG_A Cuts at: 2629 2629 Size: 5293 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5293 XmaI C'CCGG_G Cuts at: 1870 2098 2618 1870 Size: 228 520 4545 Fragments arranged by size: 4545 520 228 XmnI GAAnn'nnTTC Cuts at: 3422 3422 Size: 5293 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3101 276 Size: 53 83 186 936 1567 2468 Fragments arranged by size: 2468 1567 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI SanDI SapI SexAI SfiI SrfI SwaI XcmI