(Circular) (Six-base) MAPSORT of: pFN21AE2132.seq Check: 7920 from: 1 to: 7294 pFN21AE2132 7294 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5104 278 Size: 53 83 186 936 3568 2468 Fragments arranged by size: 3568 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2021 3237 4149 4637 1937 Size: 84 1216 912 488 4594 Fragments arranged by size: 4594 1216 912 488 84 Acc65I G'GTAC_C Cuts at: 3542 4615 3542 Size: 1073 6221 AclI AA'CG_TT Cuts at: 1563 5421 5794 1563 Size: 3858 373 3063 Fragments arranged by size: 3858 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2220 2498 3050 3378 3434 4331 4431 5357 Size: 588 278 552 328 56 897 100 926 Cuts at: 5357 6741 7009 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 926 897 588 552 328 278 268 100 56 AflII C'TTAA_G Cuts at: 820 1017 3350 820 Size: 197 2333 4764 Fragments arranged by size: 4764 2333 197 AflIII A'CryG_T Cuts at: 1203 3337 3570 3600 6193 7170 1203 Size: 2134 233 30 2593 977 1327 Fragments arranged by size: 2593 2134 1327 977 233 30 AgeI A'CCGG_T Cuts at: 6125 6125 Size: 7294 AhdI GACnn_n'nnGTC Cuts at: 6023 6023 Size: 7294 AleI CACnn'nnGTG Cuts at: 1166 2336 1166 Size: 1170 6124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2205 3702 4115 1184 Size: 1021 1497 413 4363 Fragments arranged by size: 4363 1497 1021 413 AlwNI CAG_nnn'CTG Cuts at: 3562 3790 4571 5037 6609 3562 Size: 228 781 466 1572 4247 Fragments arranged by size: 4247 1572 781 466 228 ApaLI G'TGCA_C Cuts at: 5351 6507 5351 Size: 1156 6138 ApoI r'AATT_y Cuts at: 1493 2665 3441 3500 4603 4829 4981 7090 1493 Size: 1172 776 59 1103 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 1103 776 226 152 59 AseI AT'TA_AT Cuts at: 160 1032 2604 4214 5848 160 Size: 872 1572 1610 1634 1606 Fragments arranged by size: 1634 1610 1606 1572 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7294 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2295 2894 2943 4619 1870 Size: 73 83 269 599 49 1676 4545 Fragments arranged by size: 4545 1676 599 269 83 73 49 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3526 3559 3526 Size: 33 7261 BamHI G'GATC_C Cuts at: 4624 4991 4624 Size: 367 6927 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3542 4615 6070 618 Size: 325 217 627 1755 1073 1455 1842 Fragments arranged by size: 1842 1755 1455 1073 627 325 217 BanII G_rGCy'C Cuts at: 1391 2189 2363 2979 3617 4047 4613 1391 Size: 798 174 616 638 430 566 4072 Fragments arranged by size: 4072 798 638 616 566 430 174 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2810 2841 3087 4583 928 Size: 971 911 31 246 1496 3639 Fragments arranged by size: 3639 1496 971 911 246 31 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5485 5519 1903 Size: 34 3548 34 3678 Fragments arranged by size: 3678 3548 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5186 6396 5186 Size: 1210 6084 BclI T'GATC_A Cuts at: 1548 2871 3057 1548 Size: 1323 186 5785 Fragments arranged by size: 5785 1323 186 BfrBI ATG'CAT Cuts at: 2230 2230 Size: 7294 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 3094 4127 Size: 107 122 71 631 605 365 1057 1033 Cuts at: 4127 5905 136 Size: 1778 1525 Fragments arranged by size: 1778 1525 1057 1033 631 605 365 122 107 71 BglII A'GATC_T Cuts at: 7289 7289 Size: 7294 BlpI GC'TnA_GC Cuts at: 4222 4711 4222 Size: 489 6805 Bme1580I G_kGCm'C Cuts at: 1790 2333 2935 4543 5355 6511 1790 Size: 543 602 1608 812 1156 2573 Fragments arranged by size: 2573 1608 1156 812 602 543 BmgBI CAC'GTC Cuts at: 1318 2203 3603 1318 Size: 885 1400 5009 Fragments arranged by size: 5009 1400 885 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2973 4581 5025 Size: 416 53 83 399 24 1556 1608 444 Cuts at: 5025 5983 442 Size: 958 1753 Fragments arranged by size: 1753 1608 1556 958 444 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7294 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2190 3601 5954 1239 Size: 951 1411 2353 2579 Fragments arranged by size: 2579 2353 1411 951 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5419 6284 6582 5419 Size: 865 298 6131 Fragments arranged by size: 6131 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5957 882 Size: 617 4458 2219 Fragments arranged by size: 4458 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3571 3643 5085 493 Size: 711 2367 72 1442 2702 Fragments arranged by size: 2702 2367 1442 711 72 BsaBI GATnn'nnATC Cuts at: 3518 7288 3518 Size: 3770 3524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2072 3985 5101 Size: 53 83 186 936 485 54 1913 1116 Cuts at: 5101 5483 6118 275 Size: 382 635 1451 Fragments arranged by size: 1913 1451 1116 936 635 485 382 186 83 54 53 BsaWI w'CCGG_w Cuts at: 3076 5727 6125 6399 6546 6957 3076 Size: 2651 398 274 147 411 3413 Fragments arranged by size: 3413 2651 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3017 3047 1178 Size: 30 165 30 1614 30 5425 Fragments arranged by size: 5425 1614 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4040 4052 4055 1186 Size: 2854 12 3 4425 Fragments arranged by size: 4425 2854 12 3 BseYI C'CCAG_C Cuts at: 2288 2883 3555 3806 6497 6993 2288 Size: 595 672 251 2691 496 2589 Fragments arranged by size: 2691 2589 672 595 496 251 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2349 3642 1163 Size: 573 613 1293 4815 Fragments arranged by size: 4815 1293 613 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2965 4744 5505 6533 1540 Size: 275 186 964 1779 761 1028 2301 Fragments arranged by size: 2301 1779 1028 964 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3691 4047 5355 5440 6511 3691 Size: 356 1308 85 1071 4474 Fragments arranged by size: 4474 1308 1071 356 85 BsiWI C'GTAC_G Cuts at: 3639 3639 Size: 7294 BsmI GAATG_Cn' Cuts at: 2230 4805 4898 2230 Size: 2575 93 4626 Fragments arranged by size: 4626 2575 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2104 2104 Size: 7294 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2189 2333 2363 2935 2979 3617 3691 Size: 399 399 144 30 572 44 638 74 Cuts at: 3691 4047 4543 4613 5355 5440 6511 1391 Size: 356 496 70 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 638 572 496 399 399 356 144 85 74 70 44 30 BspEI T'CCGG_A Cuts at: 3076 6957 3076 Size: 3881 3413 BspHI T'CATG_A Cuts at: 5183 6913 5183 Size: 1730 5564 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2187 4649 844 Size: 1343 2462 3489 Fragments arranged by size: 3489 2462 1343 BsrBI CCG'CTC Cuts at: 1394 1823 3033 5181 1394 Size: 429 1210 2148 3507 Fragments arranged by size: 3507 2148 1210 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2967 3795 4074 5789 5963 65 Size: 1183 1719 828 279 1715 174 1396 Fragments arranged by size: 1719 1715 1396 1183 828 279 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2043 5938 6125 1700 Size: 110 233 3895 187 2869 Fragments arranged by size: 3895 2869 233 187 110 BsrGI T'GTAC_A Cuts at: 96 2670 3573 96 Size: 2574 903 3817 Fragments arranged by size: 3817 2574 903 BssHII G'CGCG_C Cuts at: 1925 3830 1925 Size: 1905 5389 BssSI C'ACGA_G Cuts at: 5354 6366 5354 Size: 1012 6282 BstAPI GCAn_nnn'nTGC Cuts at: 794 2155 2203 4538 794 Size: 1361 48 2335 3550 Fragments arranged by size: 3550 2335 1361 48 BstBI TT'CG_AA Cuts at: 6098 6098 Size: 7294 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7294 BstXI CCAn_nnnn'nTGG Cuts at: 4276 4276 Size: 7294 BstYI r'GATC_y Cuts at: 1972 2386 4624 4991 5376 5393 6845 6931 6943 Size: 414 2238 367 385 17 1452 86 12 Cuts at: 6943 7289 1972 Size: 346 1977 Fragments arranged by size: 2238 1977 1452 414 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3963 3963 Size: 7294 BtgI C'CryG_G Cuts at: 513 1065 2035 2136 2959 4103 513 Size: 552 970 101 823 1144 3704 Fragments arranged by size: 3704 1144 970 823 552 101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6555 Fragments arranged by size: 6555 683 56 BtsI GCAGTG_nn' Cuts at: 3633 4532 4810 5604 5624 3633 Size: 899 278 794 20 5303 Fragments arranged by size: 5303 899 794 278 20 ClaI AT'CG_AT Cuts at: 1554 3723 1554 Size: 2169 5125 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7259 DraI TTT'AAA Cuts at: 2628 4598 2628 Size: 1970 5324 DraIII CAC_nnn'GTG Cuts at: 4538 4538 Size: 7294 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2723 6301 809 Size: 507 1407 3578 1802 Fragments arranged by size: 3578 1802 1407 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2962 4741 5630 Size: 54 1425 50 275 18 1132 1779 889 Cuts at: 5630 7259 8 Size: 1629 43 Fragments arranged by size: 1779 1629 1425 1132 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 4741 1537 Size: 275 1150 1779 4090 Fragments arranged by size: 4090 1779 1150 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3044 4275 5224 1353 Size: 1691 1231 949 3423 Fragments arranged by size: 3423 1691 1231 949 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5881 6255 6401 387 Size: 1064 138 4292 374 146 1280 Fragments arranged by size: 4292 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4045 4045 Size: 7294 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2220 2498 3050 3378 3434 3601 Size: 393 558 30 278 552 328 56 167 Cuts at: 3601 4331 4431 5357 5954 6741 7009 1239 Size: 730 100 926 597 787 268 1524 Fragments arranged by size: 1524 926 787 730 597 558 552 393 328 278 268 167 100 56 30 EcoNI CCTnn'n_nnAGG Cuts at: 3979 3979 Size: 7294 EcoO109I rG'GnC_Cy Cuts at: 2374 2374 Size: 7294 EcoRI G'AATT_C Cuts at: 4603 4981 4603 Size: 378 6916 FspI TGC'GCA Cuts at: 2196 5800 2196 Size: 3604 3690 HaeII r_GCGC'y Cuts at: 1678 2995 6441 1678 Size: 1317 3446 2531 Fragments arranged by size: 3446 2531 1317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2117 2149 2345 Size: 32 135 32 506 32 7 32 196 Cuts at: 2345 2377 2378 2410 2667 2699 3673 3705 3802 Size: 32 1 32 257 32 974 32 97 Cuts at: 3802 3834 5935 5967 6009 6041 1373 Size: 32 2101 32 42 32 2626 Fragments arranged by size: 2626 2101 974 506 257 196 135 97 42 32 32 32 32 32 32 32 32 32 32 32 7 1 HincII GTy'rAC Cuts at: 669 1657 1938 2022 3142 4638 4884 669 Size: 988 281 84 1120 1496 246 3079 Fragments arranged by size: 3079 1496 1120 988 281 246 84 HindIII A'AGCT_T Cuts at: 748 3062 4424 748 Size: 2314 1362 3618 Fragments arranged by size: 3618 2314 1362 HpaI GTT'AAC Cuts at: 4884 4884 Size: 7294 KpnI G_GTAC'C Cuts at: 3546 4619 3546 Size: 1073 6221 MfeI C'AATT_G Cuts at: 4893 4893 Size: 7294 MluI A'CGCG_T Cuts at: 7170 7170 Size: 7294 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2732 6408 6592 1305 Size: 1427 3676 184 2007 Fragments arranged by size: 3676 2007 1427 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5472 Fragments arranged by size: 5472 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2336 3252 4058 4097 4274 5252 5611 Size: 648 1170 916 806 39 177 978 359 Cuts at: 5611 5770 518 Size: 159 2042 Fragments arranged by size: 2042 1170 978 916 806 648 359 177 159 39 MspA1I CmG'CkG Cuts at: 1606 2037 2862 2887 2961 3559 3670 3910 4105 Size: 431 825 25 74 598 111 240 195 Cuts at: 4105 4710 5387 6535 6780 1606 Size: 605 677 1148 245 2120 Fragments arranged by size: 2120 1148 825 677 605 598 431 245 240 195 111 74 25 NaeI GCC'GGC Cuts at: 2045 2045 Size: 7294 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6742 NdeI CA'TA_TG Cuts at: 387 387 Size: 7294 NgoMIV G'CCGG_C Cuts at: 2043 2043 Size: 7294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7294 NotI GC'GGCC_GC Cuts at: 2962 4741 2962 Size: 1779 5515 NsiI A_TGCA'T Cuts at: 2232 2232 Size: 7294 NspI r_CATG'y Cuts at: 1132 1229 2490 2587 3088 4652 6197 1132 Size: 97 1261 97 501 1564 1545 2229 Fragments arranged by size: 2229 1564 1545 1261 501 97 97 PasI CC'CwG_GG Cuts at: 1348 3588 1348 Size: 2240 5054 PciI A'CATG_T Cuts at: 6193 6193 Size: 7294 PflMI CCAn_nnn'nTGG Cuts at: 1108 2318 3013 1108 Size: 1210 695 5389 Fragments arranged by size: 5389 1210 695 PfoI T'CCnGG_A Cuts at: 3362 4047 3362 Size: 685 6609 PmeI GTTT'AAAC Cuts at: 4598 4598 Size: 7294 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5363 5395 6876 6908 5363 Size: 32 1481 32 5749 Fragments arranged by size: 5749 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3984 1613 Size: 2371 4923 PsiI TTA'TAA Cuts at: 4864 4864 Size: 7294 PstI C_TGCA'G Cuts at: 830 2184 2394 3094 3567 3681 4646 830 Size: 1354 210 700 473 114 965 3478 Fragments arranged by size: 3478 1354 965 700 473 210 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7294 PvuII CAG'CTG Cuts at: 2887 3559 3670 3910 2887 Size: 672 111 240 6271 Fragments arranged by size: 6271 672 240 111 SacI G_AGCT'C Cuts at: 4047 4047 Size: 7294 SacII CC_GC'GG Cuts at: 2038 2962 4106 2038 Size: 924 1144 5226 Fragments arranged by size: 5226 1144 924 SalI G'TCGA_C Cuts at: 1936 2020 4636 1936 Size: 84 2616 4594 Fragments arranged by size: 4594 2616 84 SbfI CC_TGCA'GG Cuts at: 3094 4646 3094 Size: 1552 5742 ScaI AGT'ACT Cuts at: 5542 7254 5542 Size: 1712 5582 SexAI A'CCwGG_T Cuts at: 3999 3999 Size: 7294 SfcI C'TryA_G Cuts at: 826 1045 2180 2390 3090 3563 3677 4642 5777 Size: 219 1135 210 700 473 114 965 1135 Cuts at: 5777 6458 6649 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1135 965 700 681 473 219 210 191 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7294 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7294 SmaI CCC'GGG Cuts at: 1872 2945 4621 1872 Size: 1073 1676 4545 Fragments arranged by size: 4545 1676 1073 SmlI C'TyrA_G Cuts at: 820 1017 1943 3350 5398 6299 6561 820 Size: 197 926 1407 2048 901 262 1553 Fragments arranged by size: 2048 1553 1407 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3643 5085 493 Size: 3150 1442 2702 Fragments arranged by size: 3150 2702 1442 SpeI A'CTAG_T Cuts at: 152 152 Size: 7294 SphI G_CATG'C Cuts at: 1132 2490 4652 1132 Size: 1358 2162 3774 Fragments arranged by size: 3774 2162 1358 SspI AAT'ATT Cuts at: 5 52 5218 5 Size: 47 5166 2081 Fragments arranged by size: 5166 2081 47 StuI AGG'CCT Cuts at: 1517 2350 2909 1517 Size: 833 559 5902 Fragments arranged by size: 5902 833 559 StyI C'CwwG_G Cuts at: 513 1065 3399 4733 513 Size: 552 2334 1334 3074 Fragments arranged by size: 3074 2334 1334 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3020 5321 5338 5491 5676 6121 325 Size: 1255 1440 2301 17 153 185 445 1498 Fragments arranged by size: 2301 1498 1440 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2670 3573 3597 Size: 274 80 33 51 1443 693 903 24 Cuts at: 3597 3749 4011 5540 7252 96 Size: 152 262 1529 1712 138 Fragments arranged by size: 1712 1529 1443 903 693 274 262 152 138 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3017 3049 7254 7286 3017 Size: 32 4205 32 3025 Fragments arranged by size: 4205 3025 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3146 3790 1316 Size: 1830 644 4820 Fragments arranged by size: 4820 1830 644 XbaI T'CTAG_A Cuts at: 4630 4630 Size: 7294 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2863 3010 3602 2863 Size: 147 592 6555 Fragments arranged by size: 6555 592 147 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7294 XmaI C'CCGG_G Cuts at: 1870 2943 4619 1870 Size: 1073 1676 4545 Fragments arranged by size: 4545 1676 1073 XmnI GAAnn'nnTTC Cuts at: 2499 2703 4183 4482 5423 2499 Size: 204 1480 299 941 4370 Fragments arranged by size: 4370 1480 941 299 204 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5102 276 Size: 53 83 186 936 3568 2468 Fragments arranged by size: 3568 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI