(Linear) (Six-base) MAPSORT of: KIEE2132.seq Check: 3592 from: 1 to: 2589 KIEE2132.orf With 182 enzymes: SgfI * July 15, 2010 18:55 .. AccI GT'mk_AC Cuts at: 0 16 1232 2144 2589 Size: 16 1216 912 445 Fragments arranged by size: 1216 912 445 16 Acc65I G'GTAC_C Cuts at: 0 1537 2589 Size: 1537 1052 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 215 493 1045 1373 1429 2326 2426 2589 Size: 215 278 552 328 56 897 100 163 Fragments arranged by size: 897 552 328 278 215 163 100 56 AflII C'TTAA_G Cuts at: 0 1345 2589 Size: 1345 1244 AflIII A'CryG_T Cuts at: 0 1332 1565 1595 2589 Size: 1332 233 30 994 Fragments arranged by size: 1332 994 233 30 AleI CACnn'nnGTG Cuts at: 0 331 2589 Size: 331 2258 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 200 1697 2110 2589 Size: 200 1497 413 479 Fragments arranged by size: 1497 479 413 200 AlwNI CAG_nnn'CTG Cuts at: 0 1557 1785 2566 2589 Size: 1557 228 781 23 Fragments arranged by size: 1557 781 228 23 ApoI r'AATT_y Cuts at: 0 660 1436 1495 2589 Size: 660 776 59 1094 Fragments arranged by size: 1094 776 660 59 AseI AT'TA_AT Cuts at: 0 599 2209 2589 Size: 599 1610 380 Fragments arranged by size: 1610 599 380 AvaI C'yCGr_G Cuts at: 0 21 290 889 938 2589 Size: 21 269 599 49 1651 Fragments arranged by size: 1651 599 269 49 21 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1521 1554 2589 Size: 1521 33 1035 Fragments arranged by size: 1521 1035 33 BanI G'GyrC_C Cuts at: 0 1537 2589 Size: 1537 1052 BanII G_rGCy'C Cuts at: 0 184 358 974 1612 2042 2589 Size: 184 174 616 638 430 547 Fragments arranged by size: 638 616 547 430 184 174 BbsI GAAGACnn'nnnn_ Cuts at: 0 805 836 1082 2578 2589 Size: 805 31 246 1496 11 Fragments arranged by size: 1496 805 246 31 11 BclI T'GATC_A Cuts at: 0 866 1052 2589 Size: 866 186 1537 Fragments arranged by size: 1537 866 186 BfrBI ATG'CAT Cuts at: 0 225 2589 Size: 225 2364 BglI GCCn_nnn'nGGC Cuts at: 0 32 1089 2122 2589 Size: 32 1057 1033 467 Fragments arranged by size: 1057 1033 467 32 BlpI GC'TnA_GC Cuts at: 0 2217 2589 Size: 2217 372 Bme1580I G_kGCm'C Cuts at: 0 328 930 2538 2589 Size: 328 602 1608 51 Fragments arranged by size: 1608 602 328 51 BmgBI CAC'GTC Cuts at: 0 198 1598 2589 Size: 198 1400 991 Fragments arranged by size: 1400 991 198 BmrI ACTGGGnnnn_n' Cuts at: 0 968 2576 2589 Size: 968 1608 13 Fragments arranged by size: 1608 968 13 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 185 1596 2589 Size: 185 1411 993 Fragments arranged by size: 1411 993 185 BsaAI yAC'GTr Cuts at: 0 1566 1638 2589 Size: 1566 72 951 Fragments arranged by size: 1566 951 72 BsaBI GATnn'nnATC Cuts at: 0 1513 2589 Size: 1513 1076 BsaHI Gr'CG_yC Cuts at: 0 13 67 1980 2589 Size: 13 54 1913 609 Fragments arranged by size: 1913 609 54 13 BsaWI w'CCGG_w Cuts at: 0 1071 2589 Size: 1071 1518 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1012 1042 2589 Size: 1012 30 1547 Fragments arranged by size: 1547 1012 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2035 2047 2050 2589 Size: 2035 12 3 539 Fragments arranged by size: 2035 539 12 3 BseYI C'CCAG_C Cuts at: 0 283 878 1550 1801 2589 Size: 283 595 672 251 788 Fragments arranged by size: 788 672 595 283 251 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 1637 2589 Size: 344 1293 952 Fragments arranged by size: 1293 952 344 BsiEI CG_ry'CG Cuts at: 0 960 2589 Size: 960 1629 BsiHKAI G_wGCw'C Cuts at: 0 1686 2042 2589 Size: 1686 356 547 Fragments arranged by size: 1686 547 356 BsiWI C'GTAC_G Cuts at: 0 1634 2589 Size: 1634 955 BsmI GAATG_Cn' Cuts at: 0 225 2589 Size: 225 2364 BsmBI CGTCTCn'nnnn_ Cuts at: 0 99 2589 Size: 99 2490 Bsp1286I G_dGCh'C Cuts at: 0 184 328 358 930 974 1612 1686 2042 Size: 184 144 30 572 44 638 74 356 Cuts at: 2042 2538 2589 Size: 496 51 Fragments arranged by size: 638 572 496 356 184 144 74 51 44 30 BspEI T'CCGG_A Cuts at: 0 1071 2589 Size: 1071 1518 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 182 2589 Size: 182 2407 BsrBI CCG'CTC Cuts at: 0 1028 2589 Size: 1028 1561 BsrDI GCAATG_nn' Cuts at: 0 962 1790 2069 2589 Size: 962 828 279 520 Fragments arranged by size: 962 828 520 279 BsrFI r'CCGG_y Cuts at: 0 38 2589 Size: 38 2551 BsrGI T'GTAC_A Cuts at: 0 665 1568 2589 Size: 665 903 1021 Fragments arranged by size: 1021 903 665 BssHII G'CGCG_C Cuts at: 0 1825 2589 Size: 1825 764 BstAPI GCAn_nnn'nTGC Cuts at: 0 150 198 2533 2589 Size: 150 48 2335 56 Fragments arranged by size: 2335 150 56 48 BstXI CCAn_nnnn'nTGG Cuts at: 0 2271 2589 Size: 2271 318 BstYI r'GATC_y Cuts at: 0 381 2589 Size: 381 2208 Bsu36I CC'TnA_GG Cuts at: 0 1958 2589 Size: 1958 631 BtgI C'CryG_G Cuts at: 0 30 131 954 2098 2589 Size: 30 101 823 1144 491 Fragments arranged by size: 1144 823 491 101 30 BtsI GCAGTG_nn' Cuts at: 0 1628 2527 2589 Size: 1628 899 62 Fragments arranged by size: 1628 899 62 ClaI AT'CG_AT Cuts at: 0 1718 2589 Size: 1718 871 DraI TTT'AAA Cuts at: 0 623 2589 Size: 623 1966 DraIII CAC_nnn'GTG Cuts at: 0 2533 2589 Size: 2533 56 DrdI GACnn_nn'nnGTC Cuts at: 0 718 2589 Size: 718 1871 EaeI y'GGCC_r Cuts at: 0 957 2589 Size: 957 1632 EagI C'GGCC_G Cuts at: 0 957 2589 Size: 957 1632 EarI CTCTTCn'nnn_ Cuts at: 0 1039 2270 2589 Size: 1039 1231 319 Fragments arranged by size: 1231 1039 319 EcoICRI GAG'CTC Cuts at: 0 2040 2589 Size: 2040 549 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 185 215 493 1045 1373 1429 1596 2326 Size: 185 30 278 552 328 56 167 730 Cuts at: 2326 2426 2589 Size: 100 163 Fragments arranged by size: 730 552 328 278 185 167 163 100 56 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 1974 2589 Size: 1974 615 EcoO109I rG'GnC_Cy Cuts at: 0 369 2589 Size: 369 2220 FspI TGC'GCA Cuts at: 0 191 2589 Size: 191 2398 HaeII r_GCGC'y Cuts at: 0 990 2589 Size: 990 1599 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 73 105 112 144 340 372 373 405 Size: 73 32 7 32 196 32 1 32 Cuts at: 405 662 694 1668 1700 1797 1829 2589 Size: 257 32 974 32 97 32 760 Fragments arranged by size: 974 760 257 196 97 73 32 32 32 32 32 32 32 7 1 HincII GTy'rAC Cuts at: 0 17 1137 2589 Size: 17 1120 1452 Fragments arranged by size: 1452 1120 17 HindIII A'AGCT_T Cuts at: 0 1057 2419 2589 Size: 1057 1362 170 Fragments arranged by size: 1362 1057 170 KpnI G_GTAC'C Cuts at: 0 1541 2589 Size: 1541 1048 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 727 2589 Size: 727 1862 MslI CAynn'nnrTG Cuts at: 0 331 1247 2053 2092 2269 2589 Size: 331 916 806 39 177 320 Fragments arranged by size: 916 806 331 320 177 39 MspA1I CmG'CkG Cuts at: 0 32 857 882 956 1554 1665 1905 2100 Size: 32 825 25 74 598 111 240 195 Cuts at: 2100 2589 Size: 489 Fragments arranged by size: 825 598 489 240 195 111 74 32 25 NaeI GCC'GGC Cuts at: 0 40 2589 Size: 40 2549 NgoMIV G'CCGG_C Cuts at: 0 38 2589 Size: 38 2551 NotI GC'GGCC_GC Cuts at: 0 957 2589 Size: 957 1632 NsiI A_TGCA'T Cuts at: 0 227 2589 Size: 227 2362 NspI r_CATG'y Cuts at: 0 485 582 1083 2589 Size: 485 97 501 1506 Fragments arranged by size: 1506 501 485 97 PasI CC'CwG_GG Cuts at: 0 1583 2589 Size: 1583 1006 PflMI CCAn_nnn'nTGG Cuts at: 0 313 1008 2589 Size: 313 695 1581 Fragments arranged by size: 1581 695 313 PfoI T'CCnGG_A Cuts at: 0 1357 2042 2589 Size: 1357 685 547 Fragments arranged by size: 1357 685 547 PshAI GACnn'nnGTC Cuts at: 0 1979 2589 Size: 1979 610 PstI C_TGCA'G Cuts at: 0 179 389 1089 1562 1676 2589 Size: 179 210 700 473 114 913 Fragments arranged by size: 913 700 473 210 179 114 PvuII CAG'CTG Cuts at: 0 882 1554 1665 1905 2589 Size: 882 672 111 240 684 Fragments arranged by size: 882 684 672 240 111 SacI G_AGCT'C Cuts at: 0 2042 2589 Size: 2042 547 SacII CC_GC'GG Cuts at: 0 33 957 2101 2589 Size: 33 924 1144 488 Fragments arranged by size: 1144 924 488 33 SalI G'TCGA_C Cuts at: 0 15 2589 Size: 15 2574 SbfI CC_TGCA'GG Cuts at: 0 1089 2589 Size: 1089 1500 SexAI A'CCwGG_T Cuts at: 0 1994 2589 Size: 1994 595 SfcI C'TryA_G Cuts at: 0 175 385 1085 1558 1672 2589 Size: 175 210 700 473 114 917 Fragments arranged by size: 917 700 473 210 175 114 SmaI CCC'GGG Cuts at: 0 940 2589 Size: 940 1649 SmlI C'TyrA_G Cuts at: 0 1345 2589 Size: 1345 1244 SnaBI TAC'GTA Cuts at: 0 1638 2589 Size: 1638 951 SphI G_CATG'C Cuts at: 0 485 2589 Size: 485 2104 StuI AGG'CCT Cuts at: 0 345 904 2589 Size: 345 559 1685 Fragments arranged by size: 1685 559 345 StyI C'CwwG_G Cuts at: 0 1394 2589 Size: 1394 1195 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1015 2589 Size: 1015 1574 TatI w'GTAC_w Cuts at: 0 665 1568 1592 1744 2006 2589 Size: 665 903 24 152 262 583 Fragments arranged by size: 903 665 583 262 152 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1012 1044 2589 Size: 1012 32 1545 Fragments arranged by size: 1545 1012 32 Tth111I GACn'n_nGTC Cuts at: 0 1141 1785 2589 Size: 1141 644 804 Fragments arranged by size: 1141 804 644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 858 1005 1597 2589 Size: 858 147 592 992 Fragments arranged by size: 992 858 592 147 XmaI C'CCGG_G Cuts at: 0 938 2589 Size: 938 1651 XmnI GAAnn'nnTTC Cuts at: 0 494 698 2178 2477 2589 Size: 494 204 1480 299 112 Fragments arranged by size: 1480 494 299 204 112 Enzymes that do cut: AccI Acc65I AcuI AflII AflIII AleI AlfI AlwNI ApoI AseI AvaI BaeI BanI BanII BbsI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BpmI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII HindIII KpnI MmeI MslI MspA1I NaeI NgoMIV NotI NsiI NspI PasI PflMI PfoI PshAI PstI PvuII SacI SacII SalI SbfI SexAI SfcI SmaI SmlI SnaBI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AgeI AhdI AloI ApaI ApaLI AscI AsiSI AvrII BamHI BbeI BbvCI BcgI BciVI BglII BmtI BplI Bpu10I BpuEI BsaI BspHI BssSI BstBI BstEII BstZ17I BtgZI CspCI EciI EcoRI EcoRV FalI FseI FspAI HpaI KasI MfeI MluI MscI NarI NcoI NdeI NheI NruI PacI PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SanDI SapI ScaI SfiI SfoI SgfI SgrAI SpeI SrfI SspI SwaI XbaI XhoI ZraI