################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvaI AviII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AciI 526 GCGG CCGC(-3/-1) AflIII 546 ACATGT A^C[GA][CT]GT AhdI 755 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 755 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 403 GGACC G^G[AT]CC AvrII 27 CCTAGG C^CTAGG BbsI 49 GTCTTC GAAGAC(2/6) BlnI 27 CCTAGG C^CTAGG BpuAI 49 GTCTTC GAAGAC(2/6) BsmFI 244 GGGAC GGGAC(10/14) BspDI 787 ATCGAT AT^CGAT BspLU11I 546 ACATGT A^CATGT BsrDI 660 CATTGC GCAATG(2/0) BstBI 436 TTCGAA TT^CGAA Cfr13I 403 GGACC G^G[ACGT]CC ClaI 787 ATCGAT AT^CGAT Eam1105I 755 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 327 GAAGAG CTCTTC(1/4) EcoNI 27 CCTAGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 27 CCTAGG C^C[AT][AT]GG Ksp632I 327 GAAGAG CTCTTC(1/4) MaeII 16 ACGT A^CGT MseI 784 TTAA T^TAA NspV 436 TTCGAA TT^CGAA Sau96I 403 GGACC G^G[ACGT]CC SfaNI 160 GCATC GCATC(5/9) SfuI 436 TTCGAA TT^CGAA StuI 344 AGGCCT AGG^CCT StyI 27 CCTAGG C^C[AT][AT]GG Tru9I 784 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 118 GAATTT [GA]^AATT[CT] 565 GAATTT ApoI 118 GAATTT [GA]^AATT[CT] 565 GAATTT Asp700I 498 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 795 GAAGAACTTC BsgI 538 CTGCAC GTGCAG(16/14) 806 GTGCAG BsrI 732 ACTGG ACTGG(1/-1) 840 ACTGG BssKI 510 CCTGG ^CC[ACGT]GG 757 CCAGG BstF5I 611 CATCC GGATG(2/0) 45 GGATG BstNI 510 CCTGG CC^[AT]GG 757 CCAGG EcoRII 510 CCTGG ^CC[AT]GG 757 CCAGG FokI 611 CATCC GGATG(9/13) 45 GGATG HaeIII 345 GGCC GG^CC 432 GGCC HinfI 291 GACTC G^A[ACGT]TC 761 GAGTC HphI 591 GGTGA GGTGA(8/7) 212 TCACC MslI 541 CACACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 543 CACACATGTG MspA1I 535 CAGCTG C[AC]G^C[GT]G 632 CAGCTG MvaI 510 CCTGG CC^[AT]GG 757 CCAGG MwoI 424 GCTGTGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 625 GCTTGGACAGC NspI 546 ACATGT [GA]CATG^[CT] 674 ACATGC PleI 291 GACTC GAGTC(4/5) 761 GAGTC PvuII 535 CAGCTG CAG^CTG 632 CAGCTG ScrFI 510 CCTGG CC^[ACGT]GG 757 CCAGG XmnI 498 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 795 GAAGAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AfaI AluI BbvI BfaI BsaJI BsiYI BslI Cac8I DdeI Fnu4HI ItaI MaeI MaeIII MboII MnlI NlaIII RsaI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I