(Linear) (Six-base) MAPSORT of: KIEE5321.seq Check: 2519 from: 1 to: 849 KIEE5321.orf With 182 enzymes: SgfI * January 29, 2014 11:55 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 786 849 Size: 296 490 63 Fragments arranged by size: 490 296 63 AflIII A'CryG_T Cuts at: 0 546 849 Size: 546 303 AhdI GACnn_n'nnGTC Cuts at: 0 760 849 Size: 760 89 AleI CACnn'nnGTG Cuts at: 0 547 849 Size: 547 302 ApoI r'AATT_y Cuts at: 0 118 565 849 Size: 118 447 284 Fragments arranged by size: 447 284 118 AvrII C'CTAG_G Cuts at: 0 27 849 Size: 27 822 BbsI GAAGACnn'nnnn_ Cuts at: 0 42 849 Size: 42 807 BmrI ACTGGGnnnn_n' Cuts at: 0 742 849 Size: 742 107 Bpu10I CC'TnA_GC Cuts at: 0 41 849 Size: 41 808 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 253 283 849 Size: 253 30 566 Fragments arranged by size: 566 253 30 BseYI C'CCAG_C Cuts at: 0 419 634 849 Size: 419 215 215 Fragments arranged by size: 419 215 215 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 827 849 Size: 523 304 22 Fragments arranged by size: 523 304 22 BsrDI GCAATG_nn' Cuts at: 0 659 849 Size: 659 190 BstBI TT'CG_AA Cuts at: 0 437 849 Size: 437 412 ClaI AT'CG_AT Cuts at: 0 788 849 Size: 788 61 EarI CTCTTCn'nnn_ Cuts at: 0 322 849 Size: 322 527 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 786 849 Size: 296 490 63 Fragments arranged by size: 490 296 63 EcoNI CCTnn'n_nnAGG Cuts at: 0 31 849 Size: 31 818 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 66 463 843 849 Size: 66 397 380 6 Fragments arranged by size: 397 380 66 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 395 427 563 595 746 778 849 Size: 395 32 136 32 151 32 71 Fragments arranged by size: 395 151 136 71 32 32 32 MslI CAynn'nnrTG Cuts at: 0 545 547 849 Size: 545 2 302 Fragments arranged by size: 545 302 2 MspA1I CmG'CkG Cuts at: 0 537 634 849 Size: 537 97 215 Fragments arranged by size: 537 215 97 NspI r_CATG'y Cuts at: 0 550 678 849 Size: 550 128 171 Fragments arranged by size: 550 171 128 PasI CC'CwG_GG Cuts at: 0 510 849 Size: 510 339 PciI A'CATG_T Cuts at: 0 546 849 Size: 546 303 PvuII CAG'CTG Cuts at: 0 537 634 849 Size: 537 97 215 Fragments arranged by size: 537 215 97 StuI AGG'CCT Cuts at: 0 346 849 Size: 346 503 StyI C'CwwG_G Cuts at: 0 27 849 Size: 27 822 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 229 849 Size: 229 620 TatI w'GTAC_w Cuts at: 0 671 849 Size: 671 178 XmnI GAAnn'nnTTC Cuts at: 0 502 799 849 Size: 502 297 50 Fragments arranged by size: 502 297 50 Enzymes that do cut: AcuI AflIII AhdI AleI ApoI AvrII BbsI BmrI Bpu10I BsaXI BseYI BsgI BsrDI BstBI ClaI EarI Eco57MI EcoNI FalI Hin4I MslI MspA1I NspI PasI PciI PvuII StuI StyI TaqII TatI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI