Restriction map for pFN21AE5321
 (Linear) (Six-base) MAPSORT of: KIEE5321.seq  Check: 2519  from: 1  to: 849



KIEE5321.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     786     849 
   Size:        296     490      63

  Fragments arranged by size: 

                 490     296      63
 
AflIII A'CryG_T

Cuts at:      0     546     849 
   Size:        546     303
 
AhdI GACnn_n'nnGTC

Cuts at:      0     760     849 
   Size:        760      89
 
AleI CACnn'nnGTG

Cuts at:      0     547     849 
   Size:        547     302
 
ApoI r'AATT_y

Cuts at:      0     118     565     849 
   Size:        118     447     284

  Fragments arranged by size: 

                 447     284     118
 
AvrII C'CTAG_G

Cuts at:      0      27     849 
   Size:         27     822
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      42     849 
   Size:         42     807
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     742     849 
   Size:        742     107
 
Bpu10I CC'TnA_GC

Cuts at:      0      41     849 
   Size:         41     808
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     253     283     849 
   Size:        253      30     566

  Fragments arranged by size: 

                 566     253      30
 
BseYI C'CCAG_C

Cuts at:      0     419     634     849 
   Size:        419     215     215

  Fragments arranged by size: 

                 419     215     215
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     827     849 
   Size:        523     304      22

  Fragments arranged by size: 

                 523     304      22
 
BsrDI GCAATG_nn'

Cuts at:      0     659     849 
   Size:        659     190
 
BstBI TT'CG_AA

Cuts at:      0     437     849 
   Size:        437     412
 
ClaI AT'CG_AT

Cuts at:      0     788     849 
   Size:        788      61
 
EarI CTCTTCn'nnn_

Cuts at:      0     322     849 
   Size:        322     527
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     786     849 
   Size:        296     490      63

  Fragments arranged by size: 

                 490     296      63
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      31     849 
   Size:         31     818
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      66     463     843     849 
   Size:         66     397     380       6

  Fragments arranged by size: 

                 397     380      66       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     395     427     563     595     746     778     849 
   Size:        395      32     136      32     151      32      71

  Fragments arranged by size: 

                 395     151     136      71      32      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     545     547     849 
   Size:        545       2     302

  Fragments arranged by size: 

                 545     302       2
 
MspA1I CmG'CkG

Cuts at:      0     537     634     849 
   Size:        537      97     215

  Fragments arranged by size: 

                 537     215      97
 
NspI r_CATG'y

Cuts at:      0     550     678     849 
   Size:        550     128     171

  Fragments arranged by size: 

                 550     171     128
 
PasI CC'CwG_GG

Cuts at:      0     510     849 
   Size:        510     339
 
PciI A'CATG_T

Cuts at:      0     546     849 
   Size:        546     303
 
PvuII CAG'CTG

Cuts at:      0     537     634     849 
   Size:        537      97     215

  Fragments arranged by size: 

                 537     215      97
 
StuI AGG'CCT

Cuts at:      0     346     849 
   Size:        346     503
 
StyI C'CwwG_G

Cuts at:      0      27     849 
   Size:         27     822
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     229     849 
   Size:        229     620
 
TatI w'GTAC_w

Cuts at:      0     671     849 
   Size:        671     178
 
XmnI GAAnn'nnTTC

Cuts at:      0     502     799     849 
   Size:        502     297      50

  Fragments arranged by size: 

                 502     297      50
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AleI     ApoI    AvrII     BbsI     BmrI
   Bpu10I    BsaXI    BseYI     BsgI    BsrDI    BstBI     ClaI     EarI
  Eco57MI    EcoNI     FalI    Hin4I     MslI   MspA1I     NspI     PasI
     PciI    PvuII     StuI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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