Restriction map for
pFN21AE1794
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AfaI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbsI BbvCI BclI
BcnI BfaI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaHI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MluI MluNI MroI MscI MvnI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
PstI PvuI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 259 GTAGAC GT^[AC][GT]AC
AciI 84 GCGG CCGC(-3/-1)
AclI 421 AACGTT AA^CGTT
AcsI 110 AAATTT [GA]^AATT[CT]
Alw44I 782 GTGCAC G^TGCAC
ApaI 576 GGGCCC GGGCC^C
ApaLI 782 GTGCAC G^TGCAC
ApoI 110 AAATTT [GA]^AATT[CT]
AspHI 782 GTGCAC G[AT]GC[AT]^C
AvaII 30 GGACC G^G[AT]CC
BbrPI 638 CACGTG CAC^GTG
BcgI 135 GCAAGAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 441 AGATCT A^GATCT
BsaAI 638 CACGTG [CT]AC^GT[GA]
BsaI 697 GGTCTC GGTCTC(1/5)
BsaWI 225 ACCGGA [AT]^CCGG[AT]
BsiHKAI 782 GTGCAC G[AT]GC[AT]^C
BsmI 414 GAATGC GAATGC(1/-1)
BssSI 713 CTCGTG CACGAG(-5/-1)
BstEII 768 GGTTACC G^GT[ACGT]ACC
BstPI 768 GGTTACC G^GT[ACGT]ACC
Cac8I 646 GCTGGC GC[ACGT]^[ACGT]GC
DraI 843 TTTAAA TTT^AAA
DraII 575 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 31 GACCAGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 498 CCATGG C^C[GA][CT]GG
EarI 293 CTCTTC CTCTTC(1/4)
EcoO109I 575 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 768 GGTTACC G^GT[ACGT]ACC
HaeII 873 AGCGCC [GA]GCGC^[CT]
HapII 226 CCGG C^CGG
HgaI 87 GACGC GACGC(5/10)
HpaII 226 CCGG C^CGG
Ksp632I 293 CTCTTC CTCTTC(1/4)
MfeI 744 CAATTG C^AATTG
MslI 688 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 226 CCGG C^CGG
MunI 744 CAATTG C^AATTG
NcoI 498 CCATGG C^CATGG
PflMI 419 CCAACGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 291 GACTC GAGTC(4/5)
PmaCI 638 CACGTG CAC^GTG
PmlI 638 CACGTG CAC^GTG
Psp1406I 421 AACGTT AA^CGTT
TaqI 41 TCGA T^CGA
TfiI 124 GAATC G^A[AT]TC
TthHB8I 41 TCGA T^CGA
Van91I 419 CCAACGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 518 CCATAGACACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BanII 542 GAGCCC G[GA]GC[CT]^C
576 GGGCCC
BciVI 228 GGATAC GTATCC(6/5)
739 GGATAC
BsmAI 283 GAGAC GTCTC(1/5)
698 GTCTC
BspHI 186 TCATGA T^CATGA
753 TCATGA
BssKI 546 CCTGG ^CC[ACGT]GG
813 CCAGG
BstF5I 515 CATCC GGATG(2/0)
622 GGATG
BstNI 546 CCTGG CC^[AT]GG
813 CCAGG
BstYI 441 AGATCT [GA]^GATC[CT]
603 AGATCC
CfoI 874 GCGC GCG^C
906 GCGC
DraIII 635 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
638 CACGTGGTG
EcoRII 546 CCTGG ^CC[AT]GG
813 CCAGG
EcoT14I 20 CCAAGG C^C[AT][AT]GG
498 CCATGG
FokI 515 CATCC GGATG(9/13)
622 GGATG
HaeIII 505 GGCC GG^CC
577 GGCC
HhaI 874 GCGC GCG^C
906 GCGC
HinP1I 874 GCGC G^CGC
906 GCGC
HinfI 124 GAATC G^A[ACGT]TC
291 GACTC
MaeII 422 ACGT A^CGT
639 ACGT
MboII 294 TCTTC GAAGA(8/7)
456 TCTTC
MflI 441 AGATCT [GA]^GATC[CT]
603 AGATCC
MspA1I 46 CAGCTG C[AC]G^C[GT]G
64 CAGCTG
MvaI 546 CCTGG CC^[AT]GG
813 CCAGG
NlaIV 576 GGGCCC GG[ACGT]^[ACGT]CC
809 GGAACC
PvuII 46 CAGCTG CAG^CTG
64 CAGCTG
RcaI 186 TCATGA T^CATGA
753 TCATGA
ScrFI 546 CCTGG CC^[ACGT]GG
813 CCAGG
StyI 20 CCAAGG C^C[AT][AT]GG
498 CCATGG
Tsp45I 251 GTGAC ^GT[GC]AC
289 GTGAC
XhoII 441 AGATCT [GA]^GATC[CT]
603 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI AlwI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BstXI
Cfr13I DpnII Fnu4HI HphI ItaI
MaeIII MboI MnlI MseI NdeII
NlaIII Sau3AI Sau96I SspI Tru9I
TseI Tsp509I TspRI