Restriction map for
pFN21AE2452
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# NO CUT ENZYMES ( 130 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwNI
Aor51HI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BbeI BbrPI BcgI
BciVI BclI BfrI BlnI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AccII 221 CGCG CG^CG
AocI 1337 CCTCAGG CC^T[ACGT]AGG
AscI 219 GGCGCGCC GG^CGCGCC
BamHI 422 GGATCC G^GATCC
BbvCI 1351 GCTGAGG CCTCAGC(-5/-2)
BfaI 1573 CTAG C^TAG
BglI 1122 GCCCCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 749 AGATCT A^GATCT
BsaBI 417 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 113 GAGACC GGTCTC(1/5)
BspDI 286 ATCGAT AT^CGAT
BssHII 220 GCGCGC G^CGCGC
BstUI 221 CGCG CG^CG
BstXI 959 CCACCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1337 CCTCAGG CC^T[ACGT]AGG
ClaI 286 ATCGAT AT^CGAT
Eco81I 1337 CCTCAGG CC^T[ACGT]AGG
HaeII 89 AGCGCC [GA]GCGC^[CT]
HinfI 1456 GACTC G^A[ACGT]TC
MaeI 1573 CTAG C^TAG
MamI 417 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 213 CCGCTG C[AC]G^C[GT]G
MvnI 221 CGCG CG^CG
NcoI 1490 CCATGG C^CATGG
PleI 1456 GACTC GAGTC(4/5)
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# TWO CUT ENZYMES ( 23 enzymes) #
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AatII 556 GACGTC GACGT^C
760 GACGTC
AcyI 556 GACGTC G[GA]^CG[CT]C
760 GACGTC
AfaI 732 GTAC GT^AC
990 GTAC
ApaI 3 GGGCCC GGGCC^C
1103 GGGCCC
BsaHI 556 GACGTC G[GA]^CG[CT]C
760 GACGTC
BsgI 1324 CTGCAC GTGCAG(16/14)
1274 GTGCAG
BsmAI 113 GAGAC GTCTC(1/5)
169 GAGAC
BsrDI 143 CATTGC GCAATG(2/0)
1425 CATTGC
BsrFI 179 ACCGGC [GA]^CCGG[CT]
1142 ACCGGC
BstAPI 513 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1301 GCACCCTCTGC
Cfr10I 179 ACCGGC [GA]^CCGG[CT]
1142 ACCGGC
EaeI 527 CGGCCA [CT]^GGCC[GA]
1317 CGGCCA
FauI 975 CCCGC CCCGC(4/6)
1098 GCGGG
Hin1I 556 GACGTC G[GA]^CG[CT]C
760 GACGTC
MslI 285 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
900 CATTTCGGTG
NspI 1270 GCATGT [GA]CATG^[CT]
1394 ACATGC
PflMI 67 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
320 CCAACGTCTGG
PpuMI 401 GGGTCCC [GA]G^G[AT]CC[CT]
1341 AGGTCCC
RsaI 732 GTAC GT^AC
990 GTAC
SacI 540 GAGCTC GAGCT^C
1162 GAGCTC
SapI 183 GCTCTTC GCTCTTC(1/4)
795 GCTCTTC
Van91I 67 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
320 CCAACGTCTGG
XcmI 626 CCAAGATCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1320 CCACCTGCACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AluI Alw44I AlwI ApaLI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII