(Linear) (Six-base) MAPSORT of: KIEE2452.seq Check: 9694 from: 1 to: 1587 KIEE2452.orf With 182 enzymes: SgfI * February 5, 2013 10:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 61 1331 1445 1587 Size: 61 1270 114 142 Fragments arranged by size: 1270 142 114 61 AatII G_ACGT'C Cuts at: 0 560 764 1587 Size: 560 204 823 Fragments arranged by size: 823 560 204 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 224 685 1204 1587 Size: 224 461 519 383 Fragments arranged by size: 519 461 383 224 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 922 1442 1587 Size: 922 520 145 Fragments arranged by size: 922 520 145 ApaI G_GGCC'C Cuts at: 0 7 1107 1587 Size: 7 1100 480 Fragments arranged by size: 1100 480 7 ApaLI G'TGCA_C Cuts at: 0 511 1054 1417 1587 Size: 511 543 363 170 Fragments arranged by size: 543 511 363 170 AscI GG'CGCG_CC Cuts at: 0 220 1587 Size: 220 1367 BamHI G'GATC_C Cuts at: 0 422 1587 Size: 422 1165 BanI G'GyrC_C Cuts at: 0 228 519 712 1300 1587 Size: 228 291 193 588 287 Fragments arranged by size: 588 291 287 228 193 BanII G_rGCy'C Cuts at: 0 7 108 544 1107 1166 1587 Size: 7 101 436 563 59 421 Fragments arranged by size: 563 436 421 101 59 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 849 918 1032 1587 Size: 849 69 114 555 Fragments arranged by size: 849 555 114 69 BbvCI CC'TCA_GC Cuts at: 0 1352 1587 Size: 1352 235 BglI GCCn_nnn'nGGC Cuts at: 0 1128 1587 Size: 1128 459 BglII A'GATC_T Cuts at: 0 749 1587 Size: 749 838 BlpI GC'TnA_GC Cuts at: 0 40 86 278 1189 1587 Size: 40 46 192 911 398 Fragments arranged by size: 911 398 192 46 40 Bme1580I G_kGCm'C Cuts at: 0 7 233 508 515 524 715 1058 1107 Size: 7 226 275 7 9 191 343 49 Cuts at: 1107 1124 1303 1421 1587 Size: 17 179 118 166 Fragments arranged by size: 343 275 226 191 179 166 118 49 17 9 7 7 BmgBI CAC'GTC Cuts at: 0 246 1587 Size: 246 1341 BmrI ACTGGGnnnn_n' Cuts at: 0 125 525 684 1101 1587 Size: 125 400 159 417 486 Fragments arranged by size: 486 417 400 159 125 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 136 1587 Size: 136 1451 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 131 658 1231 1587 Size: 131 527 573 356 Fragments arranged by size: 573 527 356 131 Bpu10I CC'TnA_GC Cuts at: 0 1352 1587 Size: 1352 235 BsaI GGTCTCn'nnnn_ Cuts at: 0 107 1587 Size: 107 1480 BsaBI GATnn'nnATC Cuts at: 0 421 1587 Size: 421 1166 BsaHI Gr'CG_yC Cuts at: 0 557 761 1587 Size: 557 204 826 Fragments arranged by size: 826 557 204 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 106 136 481 511 526 556 853 883 Size: 106 30 345 30 15 30 297 30 Cuts at: 883 1048 1078 1587 Size: 165 30 509 Fragments arranged by size: 509 345 297 165 106 30 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 114 1246 1267 1270 1587 Size: 114 1132 21 3 317 Fragments arranged by size: 1132 317 114 21 3 BseYI C'CCAG_C Cuts at: 0 66 215 1069 1587 Size: 66 149 854 518 Fragments arranged by size: 854 518 149 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1295 1309 1587 Size: 1295 14 278 Fragments arranged by size: 1295 278 14 BsiHKAI G_wGCw'C Cuts at: 0 515 544 1049 1058 1166 1421 1587 Size: 515 29 505 9 108 255 166 Fragments arranged by size: 515 505 255 166 108 29 9 Bsp1286I G_dGCh'C Cuts at: 0 7 108 233 508 515 524 544 715 Size: 7 101 125 275 7 9 20 171 Cuts at: 715 1049 1058 1107 1124 1166 1303 1421 1587 Size: 334 9 49 17 42 137 118 166 Fragments arranged by size: 334 275 171 166 137 125 118 101 49 42 20 17 9 9 7 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 61 872 1001 1267 1331 1445 1587 Size: 61 811 129 266 64 114 142 Fragments arranged by size: 811 266 142 129 114 64 61 BsrBI CCG'CTC Cuts at: 0 683 795 978 1064 1587 Size: 683 112 183 86 523 Fragments arranged by size: 683 523 183 112 86 BsrDI GCAATG_nn' Cuts at: 0 142 1424 1587 Size: 142 1282 163 Fragments arranged by size: 1282 163 142 BsrFI r'CCGG_y Cuts at: 0 179 1142 1587 Size: 179 963 445 Fragments arranged by size: 963 445 179 BssHII G'CGCG_C Cuts at: 0 220 1587 Size: 220 1367 BstAPI GCAn_nnn'nTGC Cuts at: 0 519 1307 1587 Size: 519 788 280 Fragments arranged by size: 788 519 280 BstXI CCAn_nnnn'nTGG Cuts at: 0 966 1587 Size: 966 621 BstYI r'GATC_y Cuts at: 0 422 749 1505 1587 Size: 422 327 756 82 Fragments arranged by size: 756 422 327 82 Bsu36I CC'TnA_GG Cuts at: 0 1338 1587 Size: 1338 249 BtgI C'CryG_G Cuts at: 0 137 500 707 1157 1314 1490 1587 Size: 137 363 207 450 157 176 97 Fragments arranged by size: 450 363 207 176 157 137 97 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 258 1587 Size: 258 1329 BtsI GCAGTG_nn' Cuts at: 0 473 1587 Size: 473 1114 ClaI AT'CG_AT Cuts at: 0 287 1587 Size: 287 1300 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 360 395 1587 Size: 360 35 1192 Fragments arranged by size: 1192 360 35 DraIII CAC_nnn'GTG Cuts at: 0 519 896 1095 1587 Size: 519 377 199 492 Fragments arranged by size: 519 492 377 199 EaeI y'GGCC_r Cuts at: 0 527 1317 1587 Size: 527 790 270 Fragments arranged by size: 790 527 270 EarI CTCTTCn'nnn_ Cuts at: 0 190 202 499 802 1464 1587 Size: 190 12 297 303 662 123 Fragments arranged by size: 662 303 297 190 123 12 EcoICRI GAG'CTC Cuts at: 0 542 1164 1587 Size: 542 622 423 Fragments arranged by size: 622 542 423 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 131 224 658 685 1204 1231 1587 Size: 131 93 434 27 519 27 356 Fragments arranged by size: 519 434 356 131 93 27 27 EcoO109I rG'GnC_Cy Cuts at: 0 4 402 1103 1104 1334 1342 1587 Size: 4 398 701 1 230 8 245 Fragments arranged by size: 701 398 245 230 8 4 1 HaeII r_GCGC'y Cuts at: 0 93 1587 Size: 93 1494 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 106 138 257 289 1441 1473 1587 Size: 106 32 119 32 1152 32 114 Fragments arranged by size: 1152 119 114 106 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 896 1587 Size: 896 691 MslI CAynn'nnrTG Cuts at: 0 289 904 1587 Size: 289 615 683 Fragments arranged by size: 683 615 289 MspA1I CmG'CkG Cuts at: 0 215 1587 Size: 215 1372 NcoI C'CATG_G Cuts at: 0 1490 1587 Size: 1490 97 NspI r_CATG'y Cuts at: 0 1274 1398 1587 Size: 1274 124 189 Fragments arranged by size: 1274 189 124 PasI CC'CwG_GG Cuts at: 0 101 1587 Size: 101 1486 PflMI CCAn_nnn'nTGG Cuts at: 0 73 326 1587 Size: 73 253 1261 Fragments arranged by size: 1261 253 73 PfoI T'CCnGG_A Cuts at: 0 108 607 1587 Size: 108 499 980 Fragments arranged by size: 980 499 108 PpuMI rG'GwC_Cy Cuts at: 0 402 1342 1587 Size: 402 940 245 Fragments arranged by size: 940 402 245 PspOMI G'GGCC_C Cuts at: 0 3 1103 1587 Size: 3 1100 484 Fragments arranged by size: 1100 484 3 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 982 1014 1587 Size: 982 32 573 Fragments arranged by size: 982 573 32 PstI C_TGCA'G Cuts at: 0 550 1312 1367 1587 Size: 550 762 55 220 Fragments arranged by size: 762 550 220 55 SacI G_AGCT'C Cuts at: 0 544 1166 1587 Size: 544 622 421 Fragments arranged by size: 622 544 421 SanDI GG'GwC_CC Cuts at: 0 402 1587 Size: 402 1185 SapI GCTCTTCn'nnn_ Cuts at: 0 190 802 1587 Size: 190 612 785 Fragments arranged by size: 785 612 190 SfcI C'TryA_G Cuts at: 0 546 1308 1363 1587 Size: 546 762 55 224 Fragments arranged by size: 762 546 224 55 StyI C'CwwG_G Cuts at: 0 482 1346 1490 1587 Size: 482 864 144 97 Fragments arranged by size: 864 482 144 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 507 1083 1090 1587 Size: 507 576 7 497 Fragments arranged by size: 576 507 497 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 481 513 1048 1080 1587 Size: 481 32 535 32 507 Fragments arranged by size: 535 507 481 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 633 1327 1587 Size: 633 694 260 Fragments arranged by size: 694 633 260 ZraI GAC'GTC Cuts at: 0 558 762 1587 Size: 558 204 825 Fragments arranged by size: 825 558 204 Enzymes that do cut: AarI AatII AcuI AlfI ApaI ApaLI AscI BamHI BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I MmeI MslI MspA1I NcoI NspI PasI PflMI PfoI PpuMI PspOMI PsrI PstI SacI SanDI SapI SfcI StyI TaqII TstI XcmI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApoI AseI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BmtI BpuEI BsaAI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstBI BstEII BstZ17I DraI DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI XmnI