################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BcgI BclI BfrI BglII BsaBI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MluI MroI MslI NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 340 GTAGAC GT^[AC][GT]AC AccII 124 CGCG CG^CG AcyI 168 GGCGCC G[GA]^CG[CT]C AfaI 723 GTAC GT^AC Asp700I 742 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 1240 CCTAGG C^CTAGG BanI 168 GGCGCC G^G[CT][GA]CC BbeI 168 GGCGCC GGCGC^C BbrPI 680 CACGTG CAC^GTG BbsI 373 GTCTTC GAAGAC(2/6) BciVI 1167 GGATAC GTATCC(6/5) BfaI 1241 CTAG C^TAG BlnI 1240 CCTAGG C^CTAGG BpuAI 373 GTCTTC GAAGAC(2/6) BsaAI 680 CACGTG [CT]AC^GT[GA] BsaHI 168 GGCGCC G[GA]^CG[CT]C BsaWI 929 ACCGGA [AT]^CCGG[AT] BspMI 290 ACCTGC ACCTGC(4/8) BsrBI 13 GAGCGG CCGCTC(-3/-3) BssSI 1039 CTCGTG CACGAG(-5/-1) BstAPI 285 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 124 CGCG CG^CG DraIII 523 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 621 GAAGAG CTCTTC(1/4) EcoNI 1158 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 168 GGCGCC [GA]GCGC^[CT] Hin1I 168 GGCGCC G[GA]^CG[CT]C HphI 129 GGTGA GGTGA(8/7) KasI 168 GGCGCC G^GCGCC Ksp632I 621 GAAGAG CTCTTC(1/4) MaeI 1241 CTAG C^TAG MfeI 1333 CAATTG C^AATTG MseI 965 TTAA T^TAA MunI 1333 CAATTG C^AATTG MvnI 124 CGCG CG^CG NarI 168 GGCGCC GG^CGCC NruI 123 TCGCGA TCG^CGA PaeR7I 637 CTCGAG C^TCGAG PmaCI 680 CACGTG CAC^GTG PmlI 680 CACGTG CAC^GTG RsaI 723 GTAC GT^AC SfoI 168 GGCGCC GGC^GCC Tru9I 965 TTAA T^TAA XcmI 1175 CCAAAGAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 637 CTCGAG C^TCGAG XmnI 742 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BalI 272 TGGCCA TGG^CCA 332 TGGCCA BanII 544 GAGCTC G[GA]GC[CT]^C 1057 GAGCTC BbvCI 436 GCTGAGG CCTCAGC(-5/-2) 478 GCTGAGG BglI 805 GCCCGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 970 GCCTACCAGGC BlpI 180 GCTGAGC GC^T[ACGT]AGC 546 GCTCAGC BpmI 695 CTCCAG CTGGAG(16/14) 1138 CTGGAG Bpu1102I 180 GCTGAGC GC^T[ACGT]AGC 546 GCTCAGC CelII 180 GCTGAGC GC^T[ACGT]AGC 546 GCTCAGC DsaI 275 CCACGG C^C[GA][CT]GG 527 CCGTGG EaeI 272 TGGCCA [CT]^GGCC[GA] 332 TGGCCA EcoT14I 686 CCAAGG C^C[AT][AT]GG 1240 CCTAGG MaeII 681 ACGT A^CGT 707 ACGT MluNI 272 TGGCCA TGG^CCA 332 TGGCCA MscI 272 TGGCCA TGG^CCA 332 TGGCCA PflMI 198 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 522 CCACCCCGTGG SacI 544 GAGCTC GAGCT^C 1057 GAGCTC SphI 814 GCATGC GCATG^C 894 GCATGC StuI 236 AGGCCT AGG^CCT 887 AGGCCT StyI 686 CCAAGG C^C[AT][AT]GG 1240 CCTAGG Tsp509I 1148 AATT ^AATT 1334 AATT Van91I 198 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 522 CCACCCCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaI AvaII BbvI BcnI BmyI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp45I TspRI TthHB8I XhoII