(Circular) (Six-base) MAPSORT of: pFN21AE6473.seq Check: 8366 from: 1 to: 6064 pFN21AE6473 6064 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2346 3407 1937 Size: 409 1061 4594 Fragments arranged by size: 4594 1061 409 Acc65I G'GTAC_C Cuts at: 3385 3385 Size: 6064 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2408 3152 4127 5511 5779 1632 Size: 776 744 975 1384 268 1917 Fragments arranged by size: 1917 1384 975 776 744 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 4963 5940 1203 Size: 3760 977 1327 Fragments arranged by size: 3760 1327 977 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 4793 4793 Size: 6064 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AlwNI CAG_nnn'CTG Cuts at: 2296 2355 3097 3260 3807 5379 2296 Size: 59 742 163 547 1572 2981 Fragments arranged by size: 2981 1572 742 547 163 59 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 3373 3599 3751 5860 1493 Size: 1880 226 152 2109 1697 Fragments arranged by size: 2109 1880 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4618 160 Size: 872 3586 1606 Fragments arranged by size: 3586 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2642 2676 3389 1870 Size: 73 126 573 34 713 4545 Fragments arranged by size: 4545 713 573 126 73 34 AvrII C'CTAG_G Cuts at: 3245 3245 Size: 6064 BamHI G'GATC_C Cuts at: 3394 3761 3394 Size: 367 5697 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2173 3385 4840 618 Size: 325 217 627 386 1212 1455 1842 Fragments arranged by size: 1842 1455 1212 627 386 325 217 BanII G_rGCy'C Cuts at: 1391 2553 3066 3383 1391 Size: 1162 513 317 4072 Fragments arranged by size: 4072 1162 513 317 BbeI G_GCGC'C Cuts at: 2177 2177 Size: 6064 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2371 928 Size: 971 472 4621 Fragments arranged by size: 4621 971 472 BbvCI CC'TCA_GC Cuts at: 2442 2484 2442 Size: 42 6022 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4255 4289 1903 Size: 34 2318 34 3678 Fragments arranged by size: 3678 2318 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3166 3956 5166 3166 Size: 790 1210 4064 Fragments arranged by size: 4064 1210 790 BclI T'GATC_A Cuts at: 1548 1548 Size: 6064 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2816 2981 4675 Size: 107 122 71 631 605 1144 165 1694 Cuts at: 4675 136 Size: 1525 Fragments arranged by size: 1694 1525 1144 631 605 165 122 107 71 BglII A'GATC_T Cuts at: 6059 6059 Size: 6064 BlpI GC'TnA_GC Cuts at: 2186 2552 3481 2186 Size: 366 929 4769 Fragments arranged by size: 4769 929 366 Bme1580I G_kGCm'C Cuts at: 1790 4125 5281 1790 Size: 2335 1156 2573 Fragments arranged by size: 2573 2335 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2158 3795 4753 Size: 416 53 83 399 24 741 1637 958 Cuts at: 4753 442 Size: 1753 Fragments arranged by size: 1753 1637 958 741 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2685 3164 4724 1239 Size: 1446 479 1560 2579 Fragments arranged by size: 2579 1560 1446 479 Bpu10I CC'TnA_GC Cuts at: 2442 2484 2442 Size: 42 6022 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2381 2843 3299 4189 5054 5352 2381 Size: 462 456 890 865 298 3093 Fragments arranged by size: 3093 890 865 462 456 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2040 2701 2859 4727 882 Size: 617 541 661 158 1868 2219 Fragments arranged by size: 2219 1868 661 617 541 158 BsaAI yAC'GTr Cuts at: 493 1204 2687 3855 493 Size: 711 1483 1168 2702 Fragments arranged by size: 2702 1483 1168 711 BsaBI GATnn'nnATC Cuts at: 6058 6058 Size: 6064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2174 3871 4253 4888 Size: 53 83 186 936 641 1697 382 635 Cuts at: 4888 275 Size: 1451 Fragments arranged by size: 1697 1451 936 641 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2934 4497 4895 5169 5316 5727 2934 Size: 1563 398 274 147 411 3271 Fragments arranged by size: 3271 1563 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 3294 3324 1178 Size: 30 165 30 678 30 1183 30 3918 Fragments arranged by size: 3918 1183 678 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2102 2137 2459 2501 2512 1186 Size: 907 9 35 322 42 11 4738 Fragments arranged by size: 4738 907 322 42 35 11 9 BseYI C'CCAG_C Cuts at: 2035 2269 2419 2984 5267 5763 2035 Size: 234 150 565 2283 496 2336 Fragments arranged by size: 2336 2283 565 496 234 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5491 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2553 2998 3066 4125 4210 5281 2553 Size: 445 68 1059 85 1071 3336 Fragments arranged by size: 3336 1071 1059 445 85 68 BsmI GAATG_Cn' Cuts at: 3575 3668 3575 Size: 93 5971 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2553 2998 3066 3383 4125 4210 5281 Size: 399 763 445 68 317 742 85 1071 Cuts at: 5281 1391 Size: 2174 Fragments arranged by size: 2174 1071 763 742 445 399 317 85 68 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 3953 5683 3953 Size: 1730 4334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2304 3419 844 Size: 1460 1115 3489 Fragments arranged by size: 3489 1460 1115 BsrBI CCG'CTC Cuts at: 1394 1823 2020 3951 1394 Size: 429 197 1931 3507 Fragments arranged by size: 3507 1931 429 197 BsrDI GCAATG_nn' Cuts at: 65 1248 4559 4733 65 Size: 1183 3311 174 1396 Fragments arranged by size: 3311 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4708 4895 1700 Size: 110 2898 187 2869 Fragments arranged by size: 2898 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6064 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6064 BssSI C'ACGA_G Cuts at: 3044 4124 5136 3044 Size: 1080 1012 3972 Fragments arranged by size: 3972 1080 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2296 794 Size: 1502 4562 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6064 BstYI r'GATC_y Cuts at: 1972 2358 2937 3054 3193 3249 3394 3761 4146 Size: 386 579 117 139 56 145 367 385 Cuts at: 4146 4163 5615 5701 5713 6059 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 579 386 385 367 346 145 139 117 86 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 2280 2532 513 Size: 552 1215 252 4045 Fragments arranged by size: 4045 1215 552 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5325 Fragments arranged by size: 5325 683 56 BtsI GCAGTG_nn' Cuts at: 3580 4374 4394 3580 Size: 794 20 5250 Fragments arranged by size: 5250 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6064 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DraIII CAC_nnn'GTG Cuts at: 2533 2533 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 2337 3511 Size: 54 1425 50 275 18 447 60 1174 Cuts at: 3511 4400 6029 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1174 889 447 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2621 3994 1353 Size: 1268 1373 3423 Fragments arranged by size: 3423 1373 1268 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4651 5025 5171 387 Size: 1064 138 3062 374 146 1280 Fragments arranged by size: 3062 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2551 3064 2551 Size: 513 5551 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 2685 3152 3164 4127 4724 5511 Size: 393 776 277 467 12 963 597 787 Cuts at: 5511 5779 1239 Size: 268 1524 Fragments arranged by size: 1524 963 787 776 597 467 393 277 268 12 EcoNI CCTnn'n_nnAGG Cuts at: 3167 3167 Size: 6064 EcoO109I rG'GnC_Cy Cuts at: 2032 2293 2449 2589 2032 Size: 261 156 140 5507 Fragments arranged by size: 5507 261 156 140 EcoRI G'AATT_C Cuts at: 3373 3751 3373 Size: 378 5686 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2182 3293 2182 Size: 1111 4953 FspI TGC'GCA Cuts at: 4570 4570 Size: 6064 HaeII r_GCGC'y Cuts at: 1678 2177 5211 1678 Size: 499 3034 2531 Fragments arranged by size: 3034 2531 499 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2762 2794 3272 Size: 32 135 32 509 32 649 32 478 Cuts at: 3272 3294 3304 3326 4705 4737 4779 4811 1373 Size: 22 10 22 1379 32 42 32 2626 Fragments arranged by size: 2626 1379 649 509 478 135 42 32 32 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 3408 3654 669 Size: 988 281 1470 246 3079 Fragments arranged by size: 3079 1470 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 3654 3654 Size: 6064 KasI G'GCGC_C Cuts at: 2173 2173 Size: 6064 KpnI G_GTAC'C Cuts at: 3389 3389 Size: 6064 MfeI C'AATT_G Cuts at: 3338 3663 3338 Size: 325 5739 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5178 5362 1305 Size: 3873 184 2007 Fragments arranged by size: 3873 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2279 2339 10 Size: 54 1425 343 447 60 3735 Fragments arranged by size: 3735 1425 447 343 60 54 MslI CAynn'nnrTG Cuts at: 518 1166 4022 4381 4540 518 Size: 648 2856 359 159 2042 Fragments arranged by size: 2856 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2269 2320 2355 2441 2614 2734 2842 3480 Size: 663 51 35 86 173 120 108 638 Cuts at: 3480 4157 5305 5550 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 663 638 245 173 120 108 86 51 35 NarI GG'CG_CC Cuts at: 2174 2174 Size: 6064 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5512 NdeI CA'TA_TG Cuts at: 387 387 Size: 6064 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NruI TCG'CGA Cuts at: 2130 2130 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2098 2419 2823 2903 3422 4967 1132 Size: 97 869 321 404 80 519 1545 2229 Fragments arranged by size: 2229 1545 869 519 404 321 97 80 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 4963 4963 Size: 6064 PflMI CCAn_nnn'nTGG Cuts at: 1108 2209 2533 1108 Size: 1101 324 4639 Fragments arranged by size: 4639 1101 324 PfoI T'CCnGG_A Cuts at: 2928 2928 Size: 6064 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PmlI CAC'GTG Cuts at: 2687 2687 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2739 2771 4133 4165 5646 5678 2739 Size: 32 1362 32 1481 32 3125 Fragments arranged by size: 3125 1481 1362 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2449 2589 2293 Size: 156 140 5768 Fragments arranged by size: 5768 156 140 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6064 PsiI TTA'TAA Cuts at: 3634 3634 Size: 6064 PspXI vC'TCGA_Gb Cuts at: 2642 2642 Size: 6064 PstI C_TGCA'G Cuts at: 830 2292 2319 2613 2619 3416 830 Size: 1462 27 294 6 797 3478 Fragments arranged by size: 3478 1462 797 294 27 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 PvuII CAG'CTG Cuts at: 2355 2441 2614 2734 2842 2355 Size: 86 173 120 108 5577 Fragments arranged by size: 5577 173 120 108 86 SacI G_AGCT'C Cuts at: 2553 3066 2553 Size: 513 5551 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 4312 6024 4312 Size: 1712 4352 SfcI C'TryA_G Cuts at: 826 1045 2288 2315 2609 2615 3412 4547 5228 Size: 219 1243 27 294 6 797 1135 681 Cuts at: 5228 5419 826 Size: 191 1471 Fragments arranged by size: 1471 1243 1135 797 681 294 219 191 27 6 SfoI GGC'GCC Cuts at: 2175 2175 Size: 6064 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 3391 1872 Size: 1519 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2396 2642 2858 3278 4168 5069 Size: 197 926 453 246 216 420 890 901 Cuts at: 5069 5331 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 890 453 420 262 246 216 197 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 2823 2903 3422 1132 Size: 1691 80 519 3774 Fragments arranged by size: 3774 1691 519 80 SspI AAT'ATT Cuts at: 5 52 3988 5 Size: 47 3936 2081 Fragments arranged by size: 3936 2081 47 StuI AGG'CCT Cuts at: 1517 2243 2894 1517 Size: 726 651 4687 Fragments arranged by size: 4687 726 651 StyI C'CwwG_G Cuts at: 513 1065 2691 3245 3503 513 Size: 552 1626 554 258 3074 Fragments arranged by size: 3074 1626 554 552 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3089 4091 4108 4261 4446 4891 325 Size: 1255 1509 1002 17 153 185 445 1498 Fragments arranged by size: 1509 1498 1255 1002 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4310 6022 96 Size: 274 80 33 51 1443 2333 1712 138 Fragments arranged by size: 2333 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6024 6056 6024 Size: 32 6032 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6064 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3187 3187 Size: 6064 XhoI C'TCGA_G Cuts at: 1943 2642 1943 Size: 699 5365 XmaI C'CCGG_G Cuts at: 1870 3389 1870 Size: 1519 4545 XmnI GAAnn'nnTTC Cuts at: 2751 4193 2751 Size: 1442 4622 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI