Restriction map for
pFN21AE4367
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AocI ApaI AscI
AseI AsnI Asp718I AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI DraI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 135 CGCG CG^CG
AcsI 703 AAATTC [GA]^AATT[CT]
AflII 112 CTTAAG C^TTAAG
AhdI 14 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 37 AGCGCT AGC^GCT
ApoI 703 AAATTC [GA]^AATT[CT]
AspEI 14 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 69 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1101 GGGCTC G[GA]GC[CT]^C
BbsI 124 GTCTTC GAAGAC(2/6)
BfrI 112 CTTAAG C^TTAAG
BpmI 278 CTGGAG CTGGAG(16/14)
BpuAI 124 GTCTTC GAAGAC(2/6)
BsiEI 26 CGACCG CG[GA][CT]^CG
Bsp1407I 352 TGTACA T^GTACA
BsrDI 318 CATTGC GCAATG(2/0)
BsrFI 147 GCCGGT [GA]^CCGG[CT]
BsrGI 352 TGTACA T^GTACA
BssSI 797 CACGAG CACGAG(-5/-1)
BstAPI 1135 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 135 CGCG CG^CG
Cfr10I 147 GCCGGT [GA]^CCGG[CT]
CpoI 149 CGGTCCG CG^G[AT]CCG
DraII 747 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 408 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 918 GACTGTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 88 CCATGG C^C[GA][CT]GG
Eam1105I 14 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 37 AGCGCT AGC^GCT
EcoNI 231 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 747 AGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 824 CCCGC CCCGC(4/6)
HaeIII 207 GGCC GG^CC
HincII 157 GTTGAC GT[CT]^[GA]AC
HindII 157 GTTGAC GT[CT]^[GA]AC
HindIII 432 AAGCTT A^AGCTT
MseI 113 TTAA T^TAA
MslI 642 CATCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 135 CGCG CG^CG
NcoI 88 CCATGG C^CATGG
NspI 1068 ACATGC [GA]CATG^[CT]
PflFI 69 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 747 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 551 CAGCTG CAG^CTG
RsrII 149 CGGTCCG CG^G[AT]CCG
SfcI 920 CTGTAG C^T[GA][CT]AG
SspBI 352 TGTACA T^GTACA
TfiI 547 GAATC G^A[AT]TC
Tru9I 113 TTAA T^TAA
Tth111I 69 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 469 ACGTGT A^C[GA][CT]GT
756 ACGTGT
Alw44I 294 GTGCAC G^TGCAC
760 GTGCAC
AlwI 108 GATCC GGATC(4/5)
256 GGATC
ApaLI 294 GTGCAC G^TGCAC
760 GTGCAC
Asp700I 699 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1078 GAAACAATTC
AspHI 294 GTGCAC G[AT]GC[AT]^C
760 GTGCAC
AvaI 33 CTCGAG C^[CT]CG[GA]G
41 CTCGAG
BsaI 832 GAGACC GGTCTC(1/5)
867 GGTCTC
BsiHKAI 294 GTGCAC G[AT]GC[AT]^C
760 GTGCAC
BsoBI 33 CTCGAG C^[CT]CG[GA]G
41 CTCGAG
BstXI 488 CCAGAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
599 CCACCATCCTGG
BstYI 107 AGATCC [GA]^GATC[CT]
256 GGATCT
EcoT14I 88 CCATGG C^C[AT][AT]GG
885 CCAAGG
HaeII 37 AGCGCT [GA]GCGC^[CT]
50 GGCGCT
MflI 107 AGATCC [GA]^GATC[CT]
256 GGATCT
PaeR7I 33 CTCGAG C^TCGAG
41 CTCGAG
PflMI 83 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1092 CCAACTGGTGG
StyI 88 CCATGG C^C[AT][AT]GG
885 CCAAGG
TaqI 34 TCGA T^CGA
42 TCGA
TthHB8I 34 TCGA T^CGA
42 TCGA
Van91I 83 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1092 CCAACTGGTGG
XhoI 33 CTCGAG C^TCGAG
41 CTCGAG
XhoII 107 AGATCC [GA]^GATC[CT]
256 GGATCT
XmnI 699 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1078 GAAACAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TseI
Tsp45I Tsp509I TspRI