(Circular) (Six-base) MAPSORT of: pFN21AE4367.seq Check: 8310 from: 1 to: 5863 pFN21AE4367 5863 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3206 1937 Size: 1269 4594 Acc65I G'GTAC_C Cuts at: 3184 3184 Size: 5863 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2994 3926 5310 5578 1632 Size: 1362 932 1384 268 1917 Fragments arranged by size: 1917 1384 1362 932 268 AfeI AGC'GCT Cuts at: 2044 2044 Size: 5863 AflII C'TTAA_G Cuts at: 820 1017 2117 820 Size: 197 1100 4566 Fragments arranged by size: 4566 1100 197 AflIII A'CryG_T Cuts at: 1203 2474 2761 4762 5739 1203 Size: 1271 287 2001 977 1327 Fragments arranged by size: 2001 1327 1271 977 287 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 2024 4592 2024 Size: 2568 3295 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5863 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5863 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2473 2505 2473 Size: 32 5831 AlwNI CAG_nnn'CTG Cuts at: 2902 2953 2972 3146 3606 5178 2902 Size: 51 19 174 460 1572 3587 Fragments arranged by size: 3587 1572 460 174 51 19 ApaLI G'TGCA_C Cuts at: 2299 2765 3920 5076 2299 Size: 466 1155 1156 3086 Fragments arranged by size: 3086 1156 1155 466 ApoI r'AATT_y Cuts at: 1493 2708 3172 3398 3550 5659 1493 Size: 1215 464 226 152 2109 1697 Fragments arranged by size: 2109 1697 1215 464 226 152 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 2038 2046 3188 1870 Size: 73 95 8 1142 4545 Fragments arranged by size: 4545 1142 95 73 8 BamHI G'GATC_C Cuts at: 3193 3560 3193 Size: 367 5496 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3184 4639 618 Size: 325 217 627 1397 1455 1842 Fragments arranged by size: 1842 1455 1397 627 325 217 BanII G_rGCy'C Cuts at: 1391 3110 3182 1391 Size: 1719 72 4072 Fragments arranged by size: 4072 1719 72 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2122 928 Size: 971 223 4669 Fragments arranged by size: 4669 971 223 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4054 4088 1903 Size: 34 2117 34 3678 Fragments arranged by size: 3678 2117 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3755 4965 3755 Size: 1210 4653 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4474 136 Size: 107 122 71 631 605 2802 1525 Fragments arranged by size: 2802 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5858 5858 Size: 5863 BlpI GC'TnA_GC Cuts at: 3280 3280 Size: 5863 Bme1580I G_kGCm'C Cuts at: 1790 2303 2769 3924 5080 1790 Size: 513 466 1155 1156 2573 Fragments arranged by size: 2573 1156 1155 513 466 BmgBI CAC'GTC Cuts at: 1318 2475 1318 Size: 1157 4706 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2580 3594 4552 Size: 416 53 83 399 24 1163 1014 958 Cuts at: 4552 442 Size: 1753 Fragments arranged by size: 1753 1163 1014 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3160 3160 Size: 5863 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2304 4523 1239 Size: 1065 2219 2579 Fragments arranged by size: 2579 2219 1065 Bpu10I CC'TnA_GC Cuts at: 2737 2737 Size: 5863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2222 3988 4853 5151 2222 Size: 1766 865 298 2934 Fragments arranged by size: 2934 1766 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2831 2878 4526 882 Size: 617 1332 47 1648 2219 Fragments arranged by size: 2219 1648 1332 617 47 BsaAI yAC'GTr Cuts at: 493 1204 3654 493 Size: 711 2450 2702 Fragments arranged by size: 2702 2450 711 BsaBI GATnn'nnATC Cuts at: 5857 5857 Size: 5863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3670 4052 4687 275 Size: 53 83 186 936 2137 382 635 1451 Fragments arranged by size: 2137 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4296 4694 4968 5115 5526 4296 Size: 398 274 147 411 4633 Fragments arranged by size: 4633 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2082 2112 2234 2264 3005 Size: 30 165 30 679 30 122 30 741 Cuts at: 3005 3035 1178 Size: 30 4006 Fragments arranged by size: 4006 741 679 165 122 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 2261 2606 2763 2954 3001 3004 1186 Size: 1072 3 345 157 191 47 3 4045 Fragments arranged by size: 4045 1072 345 191 157 47 3 3 BseYI C'CCAG_C Cuts at: 2199 2581 5066 5562 2199 Size: 382 2485 496 2500 Fragments arranged by size: 2500 2485 496 382 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 3313 4074 5102 1540 Size: 275 186 33 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 2303 2769 3924 4009 5080 2303 Size: 466 1155 85 1071 3086 Fragments arranged by size: 3086 1155 1071 466 85 BsmI GAATG_Cn' Cuts at: 3374 3467 3374 Size: 93 5770 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2303 2769 3110 3182 3924 4009 5080 Size: 399 513 466 341 72 742 85 1071 Cuts at: 5080 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 513 466 399 341 85 72 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 3752 5482 3752 Size: 1730 4133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3218 844 Size: 2374 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3750 1394 Size: 429 1927 3507 Fragments arranged by size: 3507 1927 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2322 4358 4532 65 Size: 1183 1074 2036 174 1396 Fragments arranged by size: 2036 1396 1183 1074 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2152 4507 4694 1700 Size: 110 342 2355 187 2869 Fragments arranged by size: 2869 2355 342 187 110 BsrGI T'GTAC_A Cuts at: 96 2357 96 Size: 2261 3602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 2802 3923 4935 2802 Size: 1121 1012 3730 Fragments arranged by size: 3730 1121 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3146 794 Size: 2352 3511 BstBI TT'CG_AA Cuts at: 4667 4667 Size: 5863 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5863 BstXI CCAn_nnnn'nTGG Cuts at: 2500 2611 2500 Size: 111 5752 BstYI r'GATC_y Cuts at: 1972 2112 2261 3193 3560 3945 3962 5414 5500 Size: 140 149 932 367 385 17 1452 86 Cuts at: 5500 5512 5858 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 932 385 367 346 149 140 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2093 513 Size: 552 939 89 4283 Fragments arranged by size: 4283 939 552 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5124 Fragments arranged by size: 5124 683 56 BtsI GCAGTG_nn' Cuts at: 3379 4173 4193 3379 Size: 794 20 5049 Fragments arranged by size: 5049 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 3167 3167 Size: 5863 DraIII CAC_nnn'GTG Cuts at: 2418 2418 Size: 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2929 4870 809 Size: 507 1613 1941 1802 Fragments arranged by size: 1941 1802 1613 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3310 4199 5828 Size: 54 1425 50 275 18 1480 889 1629 Cuts at: 5828 8 Size: 43 Fragments arranged by size: 1629 1480 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3310 1537 Size: 275 1498 4090 Fragments arranged by size: 4090 1498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3793 1353 Size: 2440 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2831 4450 4824 4970 387 Size: 1064 138 1242 1619 374 146 1280 Fragments arranged by size: 1619 1280 1242 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2304 2994 3926 4523 5310 5578 1239 Size: 393 672 690 932 597 787 268 1524 Fragments arranged by size: 1524 932 787 690 672 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2240 2240 Size: 5863 EcoO109I rG'GnC_Cy Cuts at: 2753 2753 Size: 5863 EcoRI G'AATT_C Cuts at: 3172 3550 3172 Size: 378 5485 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 2046 2059 5010 1678 Size: 368 13 2951 2531 Fragments arranged by size: 2951 2531 368 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2010 2042 2082 2114 2438 Size: 32 135 32 438 32 40 32 324 Cuts at: 2438 2470 2487 2519 3005 3037 4504 4536 4578 Size: 32 17 32 486 32 1467 32 42 Cuts at: 4578 4610 1373 Size: 32 2626 Fragments arranged by size: 2626 1467 486 438 324 135 42 40 32 32 32 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 2164 3207 3453 669 Size: 988 281 226 1043 246 3079 Fragments arranged by size: 3079 1043 988 281 246 226 HindIII A'AGCT_T Cuts at: 748 2437 748 Size: 1689 4174 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KpnI G_GTAC'C Cuts at: 3188 3188 Size: 5863 MfeI C'AATT_G Cuts at: 3462 3462 Size: 5863 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2355 2681 4977 5161 1305 Size: 1050 326 2296 184 2007 Fragments arranged by size: 2296 2007 1050 326 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4041 Fragments arranged by size: 4041 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2651 3821 4180 4339 518 Size: 648 1485 1170 359 159 2042 Fragments arranged by size: 2042 1485 1170 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2558 2588 3026 3279 3956 5104 5349 1606 Size: 952 30 438 253 677 1148 245 2120 Fragments arranged by size: 2120 1148 952 677 438 253 245 30 NcoI C'CATG_G Cuts at: 513 1065 2004 2093 513 Size: 552 939 89 4283 Fragments arranged by size: 4283 939 552 89 NdeI CA'TA_TG Cuts at: 387 387 Size: 5863 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 3077 3221 4766 1132 Size: 97 1848 144 1545 2229 Fragments arranged by size: 2229 1848 1545 144 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5863 PciI A'CATG_T Cuts at: 4762 4762 Size: 5863 PflMI CCAn_nnn'nTGG Cuts at: 1108 2094 3103 1108 Size: 986 1009 3868 Fragments arranged by size: 3868 1009 986 PfoI T'CCnGG_A Cuts at: 2062 2100 2442 2062 Size: 38 342 5483 Fragments arranged by size: 5483 342 38 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3964 5445 5477 3932 Size: 32 1481 32 4318 Fragments arranged by size: 4318 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2753 2753 Size: 5863 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5863 PsiI TTA'TAA Cuts at: 3433 3433 Size: 5863 PspXI vC'TCGA_Gb Cuts at: 2038 2046 2038 Size: 8 5855 PstI C_TGCA'G Cuts at: 830 3215 830 Size: 2385 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 PvuII CAG'CTG Cuts at: 2558 2558 Size: 5863 RsrII CG'GwC_CG Cuts at: 2155 2155 Size: 5863 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 4111 5823 4111 Size: 1712 4151 SfcI C'TryA_G Cuts at: 826 1045 2925 3211 4346 5027 5218 826 Size: 219 1880 286 1135 681 191 1471 Fragments arranged by size: 1880 1471 1135 681 286 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 3190 1872 Size: 1318 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2038 2046 2117 2237 3967 4868 Size: 197 926 95 8 71 120 1730 901 Cuts at: 4868 5130 820 Size: 262 1553 Fragments arranged by size: 1730 1553 926 901 262 197 120 95 71 8 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 3221 1132 Size: 2089 3774 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5863 StyI C'CwwG_G Cuts at: 513 1065 2004 2093 2890 3302 513 Size: 552 939 89 797 412 3074 Fragments arranged by size: 3074 939 797 552 412 89 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2882 3890 3907 4060 4245 4690 325 Size: 1255 1302 1008 17 153 185 445 1498 Fragments arranged by size: 1498 1302 1255 1008 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2357 4109 5821 Size: 274 80 33 51 1443 380 1752 1712 Cuts at: 5821 96 Size: 138 Fragments arranged by size: 1752 1712 1443 380 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2234 2266 5823 5855 2234 Size: 32 3557 32 2242 Fragments arranged by size: 3557 2242 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2077 1316 Size: 761 5102 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XhoI C'TCGA_G Cuts at: 1943 2038 2046 1943 Size: 95 8 5760 Fragments arranged by size: 5760 95 8 XmaI C'CCGG_G Cuts at: 1870 3188 1870 Size: 1318 4545 XmnI GAAnn'nnTTC Cuts at: 2708 3087 3992 2708 Size: 379 905 4579 Fragments arranged by size: 4579 905 379 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI